RESUMEN
ization and unification of diseases of the oral mucosa in patients who underwent Covid-19, in order to increase the effectiveness of qualified dental care for this group of people in a pandemic and create conditions for continuous professional development of dentists. 42 patients were attended, who came through Covid-19, aged 36 to 70 years, who applied independently or were referred by doctors of private clinics in the city of Ternopil. The gender distribution of patients was 19 males and 23 females. Clinical examination of patients with acute respiratory viral infection Сovid-19, at the stage of subjective methods, revealed the following complaints: loss of taste was found in 38 people (90%) patients, salivation disorders (dry mouth or hypersalivation) - in 40 people (95%) patients, burning sensation, discomfort and «creeping ants¼ in the tongue or other anatomical components of the oral mucosa in 29 people (69%) patients. Complaints about the integrity of the oral mucosa and the associated pain were associated with pain in 13 people (31%). Patients diagnosed with Covid-19 were diagnosed with glossodynia 7 individuals, (17%), candidal stomatitis 12 individuals, (28%), herpetic stomatitis 5 individuals, (12%), and chronic recurrent aphthous stomatitis 8 individuals, (19%), desquamative glossitis in 10 people (24%). In some clinical cases, 10 people (24%) observed a combination of nosological units. The obtained results substantiate the development of the necessary algorithm of dental examination of dental data of patients in a pandemic.
Asunto(s)
COVID-19 , Mucosa Bucal , Odontólogos , Femenino , Humanos , Masculino , Pandemias , SARS-CoV-2 , Organización Mundial de la SaludRESUMEN
This corrects the article DOI: 10.1103/PhysRevLett.117.182502.
RESUMEN
Different allelic variants of genes that encode ATP-sensitive potassium (KATP ) channels' subunits may contribute to the development of heart failure. The purpose of the work to investigate SNPs in genes that encode KATP channels in relation to echocardiographic parameters in chronic heart failure (CHF) patients. Ninety-nine people with CHF of ischemic origin with left ventricular systolic dysfunction were examined. The control group is represented by 108 clinically healthy subjects. KCNJ11 polymorphisms Ile337Val and Glu23Lys, and ABCC8 polymorphism Ser1369Ala were genotyped using polymerase chain reaction. In CHF patients, the frequency of the Ile337Val genotype was: Ile/Ile, 40.4%; Ile/Val, 45.5%; and Val/Val, 14.1%. The patients with the Val/Val genotype had left ventricular (LV) mass that was 334.15 g, which was 27.3% (P < 0.05) lower versus Ile/Val patients (425.48 g). The index of this parameter was also significantly lower (28.4%, P < 0.05). In CHF patients, the frequency of Glu23Lys and Ser1369Ala was: Glu/Glu and Ser/Ser, 43.4%; heterozygote, 44.4%; Lys/Lys and Ala/Ala, 12.2%. The patients with the Lys/Lys and Ala/Ala genotypes had a significantly lower LV mass index and LV end-diastolic volume (22.9% and 26.8%, P < 0.05) versus heterozygotes. Thus, the greatest LV mass and LV end-diastolic volume values are associated with heterozygotes, while the smallest are associated with minor homozygotes.
Asunto(s)
Ecocardiografía , Insuficiencia Cardíaca/genética , Canales de Potasio de Rectificación Interna/genética , Receptores de Sulfonilureas/genética , Anciano , Alelos , Estudios de Casos y Controles , Femenino , Frecuencia de los Genes , Genotipo , Insuficiencia Cardíaca/diagnóstico , Humanos , Masculino , Persona de Mediana Edad , Ucrania , Disfunción Ventricular Izquierda/genéticaRESUMEN
We utilize various ab initio approaches to search for a low-lying resonance in the four-neutron (4n) system using the JISP16 realistic NN interaction. Our most accurate prediction is obtained using a J-matrix extension of the no-core shell model and suggests a 4n resonant state at an energy near E_{r}=0.8 MeV with a width of approximately Γ=1.4 MeV.
RESUMEN
Andreeva Bay in northwest Russia hosts one of the former coastal technical bases of the Northern Fleet. Currently, this base is designated as the Andreeva Bay branch of Northwest Center for Radioactive Waste Management (SevRAO) and is a site of temporary storage (STS) for spent nuclear fuel (SNF) and other radiological waste generated during the operation and decommissioning of nuclear submarines and ships. According to an integrated expert evaluation, this site is the most dangerous nuclear facility in northwest Russia. Environmental rehabilitation of the site is currently in progress and is supported by strong international collaboration. This paper describes how the optimization principle (ALARA) has been adopted during the planning of remediation work at the Andreeva Bay STS and how Russian-Norwegian collaboration greatly contributed to ensuring the development and maintenance of a high level safety culture during this process. More specifically, this paper describes how integration of a system, specifically designed for improving the radiological safety of workers during the remediation work at Andreeva Bay, was developed in Russia. It also outlines the 3D radiological simulation and virtual reality based systems developed in Norway that have greatly facilitated effective implementation of the ALARA principle, through supporting radiological characterisation, work planning and optimization, decision making, communication between teams and with the authorities and training of field operators.
Asunto(s)
Sitios de Residuos Peligrosos , Imagenología Tridimensional/métodos , Modelos Organizacionales , Cultura Organizacional , Protección Radiológica/métodos , Residuos Radiactivos/prevención & control , Administración de la Seguridad/organización & administración , Descontaminación/métodos , Noruega , Monitoreo de Radiación/métodos , Federación de RusiaRESUMEN
The supramolecular compound calix[4]arene C-90 (5,11,17,23-tetra(trifluoro)methyl(phenylsulfonylimino)-methylamino-25,26,27,28-tetrapropoxycalix[4]arene) is shown to efficiently inhibit the ATP hydrolase activity of Ca2+,Mg2+-ATPase in the myometrium cell plasma membrane fraction and also in a preparation of the purified enzyme solubilized from this subcellular fraction. The inhibition coefficient I0.5 values were 20.2 ± 0.5 and 58.5 ± 6.4 µM for the membrane fraction and the solubilized enzyme, respectively. The inhibitory effect of calix[4]arene C-90 was selective comparatively to other ATPases localized in the plasma membrane: calix[4]arene C-90 did not influence the activities of Na+,K+-ATPase and "basal" Mg2+-ATPase. The inhibitory effect of calix[4]arene C-90 on the Ca2+,Mg2+-ATPase activity was associated with the cooperative action of four trifluoromethylphenylsulfonylimine (sulfonylamidine) groups oriented similarly on the upper rim of the calix[4]arene macrocycle (the calix[4]arene "bowl"). The experimental findings seem to be of importance for studies, using calix[4]arene C-90, of membrane mechanisms of regulation of calcium homeostasis in smooth muscle cells and also for investigation of the participation of the plasma membrane Ca2+-pump in control of electro- and pharmacomechanical coupling in myocytes.
Asunto(s)
ATPasa de Ca(2+) y Mg(2+)/antagonistas & inhibidores , Calixarenos/química , Membrana Celular/enzimología , Miometrio/metabolismo , Fenoles/química , Animales , ATPasa de Ca(2+) y Mg(2+)/metabolismo , Calixarenos/síntesis química , Calixarenos/metabolismo , Membrana Celular/metabolismo , Femenino , Cinética , Miocitos del Músculo Liso/enzimología , Miocitos del Músculo Liso/metabolismo , Miometrio/citología , Fenoles/síntesis química , Fenoles/metabolismo , Unión Proteica , PorcinosRESUMEN
Chronic (15 days) single daily intraperitoneal insertion of the new preparation MT (5 mg/kg) and metoprolol (10 mg/kg) into SHR rats leads to the same decrease (18%) in arterial pressure. In addition, MT exhibits a cardioprotective effect because of NO-mimetic properties, increasing NO formation in myocardium via increasing general NOS activity and eNOS expression. MT normalizes iNOS expression in myocardium mitochondria and decreases nitrotyrosine (nitrosation stress marker) formation. At the same time, the reference preparation metoprolol did not exhibit NO-mimetic properties in myocardium of SHR rats.
Asunto(s)
Antiarrítmicos/farmacología , Cardiotónicos/farmacología , Metoprolol/farmacología , Óxido Nítrico/metabolismo , Triazoles/farmacología , Animales , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Miocardio/enzimología , Óxido Nítrico Sintasa de Tipo III/biosíntesis , Ratas , Ratas Endogámicas SHRRESUMEN
The article presents data regarding links of I/D angiotensin-converting enzyme (ACE) gene polymorphism and structural changes in the myocardium and predictive value in chronic heart failure (CHF). We did not find association of I/D ACE gene polymorphism and structural changes in the myocardium and predictive value in patients with CHF, receiving treatment inhibitor ACE.
Asunto(s)
Insuficiencia Cardíaca/genética , Hipertensión/genética , Peptidil-Dipeptidasa A/genética , Polimorfismo Genético , Sistema Renina-Angiotensina/efectos de los fármacos , Anciano , Sustitución de Aminoácidos , Inhibidores de la Enzima Convertidora de Angiotensina/farmacología , Inhibidores de la Enzima Convertidora de Angiotensina/uso terapéutico , Antihipertensivos/farmacología , Antihipertensivos/uso terapéutico , Ácido Aspártico/genética , Femenino , Genotipo , Insuficiencia Cardíaca/tratamiento farmacológico , Insuficiencia Cardíaca/mortalidad , Insuficiencia Cardíaca/patología , Humanos , Hipertensión/tratamiento farmacológico , Hipertensión/mortalidad , Hipertensión/patología , Isoleucina/genética , Masculino , Persona de Mediana Edad , Miocardio/patología , Reacción en Cadena de la Polimerasa , Valor Predictivo de las Pruebas , Pronóstico , Sistema Renina-Angiotensina/genética , Análisis de SupervivenciaRESUMEN
Apoptosis, a physiologically critical process, is characterized by a destruction of the cell after sequential degradation of key cellular components. Here, we set out to explore the fate of the physiologically indispensable nuclear envelope (NE) in this process. The NE mediates the critical nucleocytoplasmic transport through nuclear pore complexes (NPCs). In addition, the NE is involved in gene expression and contributes significantly to the overall structure and mechanical stability of the cell nucleus through the nuclear lamina, which underlies the entire nucleoplasmic face of the NE and thereby interconnects the NPCs, the NE, and the genomic material. Using the nano-imaging and mechanical probing approach atomic force microscopy (AFM) and biochemical methods, we unveiled the fate of the NE during apoptosis. The doomed NE sustains a degradation of both the mediators of the critical selective nucleocytoplasmic transport, namely NPC cytoplasmic filaments and basket, and the nuclear lamina. These observations are paralleled by marked softening and destabilization of the NE and the detection of vesicle-like nuclear fragments. We conclude that destruction of the cell nucleus during apoptosis proceeds in a strategic fashion. Degradation of NPC cytoplasmic filaments and basket shuts down the critical selective nucleocytoplasmic cross-talk. Degradation of the nuclear lamina disrupts the pivotal connection between the NE and the chromatin, breaks up the overall nuclear architecture, and softens the NE, thereby enabling the formation of nuclear fragments at later stages of apoptosis.
Asunto(s)
Apoptosis/fisiología , Cromatina/metabolismo , Lámina Nuclear/metabolismo , Poro Nuclear/metabolismo , Transporte Activo de Núcleo Celular/fisiología , Animales , Células Cultivadas , Microscopía de Fuerza Atómica/métodos , Lámina Nuclear/ultraestructura , Poro Nuclear/ultraestructura , Xenopus laevisRESUMEN
The SPASIBA force field has been applied to the determination of the structure and dynamical properties of various disaccharides. It has been shown that the experimental properties (structure, dipole moment, conformational relative energies) are satisfactorily predicted. The anomeric and exo-anomeric effects are confidently reproduced without specific terms for the alpha and beta anomers and the type of glycosidic linkages.
Asunto(s)
Algoritmos , Conformación de Carbohidratos , Disacáridos/química , Glicósidos/química , Programas Informáticos , Electricidad Estática , Estereoisomerismo , Relación Estructura-Actividad , Compuestos de Sulfhidrilo/química , Termodinámica , Trehalosa/químicaRESUMEN
The changes in protein-protein interactions mediated by SH3 domain of the regulatory p85 alpha subunit of phosphatidylinositol 3-kinase (Pl 3-kinase) in the course of herbimycin A-induced erythroid differentiation of the human erythroleukemia cell line K562 have been analyzed. Binding assay was performed in vitro using a recombinant form of SH3 domain of p85 alpha, conjugated with glutathione-S-transferase. pTyr-containing 210, 116, 52 and 46 kDa proteins, binding of which are modulated in differentiated cells, were identified and binding dynamics was analysed. The obtained data on binding the specific pTyr-containing proteins with regulatory subunit of Pl 3-kinase testify about the coordinated control of Pl 3-kinase signalling pathway in the course of herbimycin A-induced K562 cells differentiation.
Asunto(s)
Antibacterianos/farmacología , Diferenciación Celular/efectos de los fármacos , Eritrocitos/efectos de los fármacos , Fosfatidilinositol 3-Quinasas/metabolismo , Quinonas/farmacología , Transducción de Señal , Benzoquinonas , Eritrocitos/citología , Glutatión Transferasa/metabolismo , Humanos , Células K562 , Lactamas Macrocíclicas , Unión Proteica , Proteínas Recombinantes de Fusión/metabolismo , Rifabutina/análogos & derivados , Dominios Homologos srcRESUMEN
The neuroleptic, analgesic, antiinflammatory, antihypoxic, diuretic, bacteriostatic, and fungistatic activity of bicyclic noncondensed thiazolidons-2 with alkylene and aryl bridges was studied. Certain regularities in the relations between the structure of the compounds under study and their biological activity were found.
Asunto(s)
Tiazoles/farmacología , Analgésicos/farmacología , Analgésicos/toxicidad , Animales , Antiinfecciosos/farmacología , Antiinfecciosos/toxicidad , Antiinflamatorios/farmacología , Antiinflamatorios/toxicidad , Antifúngicos/farmacología , Antifúngicos/toxicidad , Antipsicóticos/farmacología , Antipsicóticos/toxicidad , Diuréticos/farmacología , Diuréticos/toxicidad , Femenino , Masculino , Ratones , Ratas , Relación Estructura-Actividad , Tiazoles/toxicidadRESUMEN
Antioxidant and antiradical activity of 1,2,4-triazole and quinazoline derivatives inhibiting superradical at the initial stages of free-radical oxidation have been studied in vitro by the method of Hara P, Mista, 1972. It is acceptable for the determination of antiradical and antioxidant activity of newly synthesized compounds.
Asunto(s)
Antioxidantes/uso terapéutico , Depuradores de Radicales Libres/uso terapéutico , Ataque Isquémico Transitorio/tratamiento farmacológico , Quinazolinas/uso terapéutico , Triazoles/uso terapéutico , Animales , Estructura Molecular , Ratas , Ratas Wistar , Triazoles/químicaRESUMEN
The new antioxidant Thiotriazoline was used in the treatment of patients with retinal vascular occlusive processes. The agent improved retinal metabolism by inhibiting free radical oxidation. Thiotriazoline is superior to alpha-tocopherol acetate in its activity. Unlike the latter, it has no vasodilating effect.
Asunto(s)
Antioxidantes/uso terapéutico , Ojo/irrigación sanguínea , Isquemia/tratamiento farmacológico , Triazoles/uso terapéutico , Enfermedad Aguda , Evaluación de Medicamentos , Quimioterapia Combinada , Radicales Libres , Humanos , Isquemia/sangre , Nervio Óptico/irrigación sanguínea , Enfermedades del Nervio Óptico/sangre , Enfermedades del Nervio Óptico/tratamiento farmacológico , Oxidación-Reducción/efectos de los fármacos , Enfermedades de la Retina/sangre , Enfermedades de la Retina/tratamiento farmacológico , Vena Retiniana , Trombosis/sangre , Trombosis/tratamiento farmacológicoAsunto(s)
Barrera Hematoencefálica/efectos de los fármacos , Trastornos Cerebrovasculares/tratamiento farmacológico , Trastornos Cerebrovasculares/fisiopatología , Hipertensión/tratamiento farmacológico , Hipertensión/fisiopatología , Animales , Antioxidantes/uso terapéutico , Hemorragia Cerebral/tratamiento farmacológico , Hemorragia Cerebral/etiología , Hemorragia Cerebral/fisiopatología , Trastornos Cerebrovasculares/etiología , Evaluación Preclínica de Medicamentos , Humanos , Hidrazinas/uso terapéutico , Hipertensión/complicaciones , Masculino , Quinazolinas/uso terapéutico , Quinazolinonas , Ratas , Ratas WistarAsunto(s)
Anemia/etiología , Anemia/terapia , Eritropoyetina/uso terapéutico , Síndrome de Inmunodeficiencia Adquirida/complicaciones , Artritis Reumatoide/complicaciones , Enfermedades Autoinmunes/complicaciones , Donantes de Sangre , Enfermedades Hematológicas/complicaciones , Humanos , Fallo Renal Crónico/complicaciones , Neoplasias/complicaciones , Proteínas Recombinantes/uso terapéuticoRESUMEN
It is established that KS-79 compound possesses the high level of antioxidant activity and realizes its antioxidant effect at initial stages of free-radical hydrogen peroxide oxidation of lipids. Due to this ability it differs from the oxidants of direct-type action.
Asunto(s)
Antioxidantes/farmacología , Hidrazinas/farmacología , Hipoxia Encefálica/tratamiento farmacológico , Quinazolinas/farmacología , Animales , Isquemia Encefálica/tratamiento farmacológico , Isquemia Encefálica/metabolismo , Radicales Libres , Hipoxia Encefálica/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Masculino , Quinazolinonas , Ratas , Ratas Wistar , Vitamina E/metabolismoRESUMEN
The effect of quinazopyrine on skin wound healing in rats was studied. Quinazopyrine was found to stimulate synthesis of nucleic acids, maturation of fibroblasts and to increase mechanical strength of the postoperative scar. This effect retains in animals with alloxan-induced diabetes. The wound-healing activity of quinazopyrine was higher than that of methyluracil, dimexide, pentoxyl, sodium nucleinate, potassium orotate and riboxine.