RESUMEN

Galectin-3 has been implicated in tumor progression. We demonstrated immunohistochemically that galectin-3 was negative in normal breast tissue, but it was highly increased in breast cancer and in metastatic tissues to brain. Similarly, histochemistry with mucin-specific lectins showed increased recognition in breast tumor and metastasis with Machaerocereus eruca agglutinin (Fualpha 1,2 (GalNAcalpha 1,3) Galss1,4 in complex mucin) but not for Amaranthus leucocarpus (Galss1,3-GalNAc-alpha 1,0-Ser/Thr) and Arachis hypogaea lectins (Galss1,3GalNAc/Galss1,4GlcNAc). Mucin-type glycans and galectin-3 colocalized in breast cancer and metastasis, but not in normal tissue, suggesting upregulated biosynthesis of complex O-glycosidically linked glycans and galectin-3 favor breast cancer progression and brain metastasis.

Asunto(s)

Neoplasias Encefálicas/química , Neoplasias de la Mama/química , Galectina 3/análisis , Mucinas/análisis , Arachis , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/secundario , Neoplasias de la Mama/metabolismo , Neoplasias de la Mama/patología , Femenino , Glicoproteínas , Glicosilación , Histocitoquímica/métodos , Humanos , Inmunohistoquímica , Mucinas/metabolismo , Neuraminidasa/metabolismo , Lectinas de Plantas , Manejo de Especímenes/métodos , Tripsina/metabolismo , Regulación hacia Arriba