RESUMEN
Homologous recombination (HR) requires nuclease activities at multiple steps, but the contribution of individual nucleases to the processing of double-strand DNA ends at different stages of HR has not been clearly defined. We used chicken DT40 cells to investigate the role of flap endonuclease 1 (Fen-1) in HR. FEN-1-deficient cells exhibited a significant decrease in the efficiency of immunoglobulin gene conversion while being proficient in recombination between sister chromatids, suggesting that Fen-1 may play a role in HR between sequences of considerable divergence. To clarify whether sequence divergence at DNA ends is truly the reason for the observed HR defect in FEN-1(-/-) cells we inserted a unique I-SceI restriction site in the genome and tested various donor and recipient HR substrates. We found that the efficiency of HR-mediated DNA repair was indeed greatly diminished when divergent sequences were present at the DNA break site. We conclude that Fen-1 eliminates heterologous sequences at DNA damage site and facilitates DNA repair by HR.
Asunto(s)
Daño del ADN , Endonucleasas de ADN Solapado/fisiología , Recombinación Genética , Animales , Secuencia de Bases , Ciclo Celular , Pollos , ADN/química , Reparación del ADN , ADN Complementario/metabolismo , Desoxirribonucleasas de Localización Especificada Tipo II/farmacología , Endonucleasas de ADN Solapado/metabolismo , Citometría de Flujo , Rayos gamma , Cinética , Modelos Genéticos , Datos de Secuencia Molecular , Mutación , Plásmidos/metabolismo , Proteínas de Saccharomyces cerevisiae , Homología de Secuencia de Ácido Nucleico , Intercambio de Cromátides Hermanas , Factores de Tiempo , TransfecciónRESUMEN
Flap endonuclease 1 (FEN-1) is a nuclear enzyme involved in DNA metabolism, such as replication, repair, and recombination. Here, we report the comparative genomic organization of the chicken, mouse, and human FEN1 genes as well as the comparative organization of a small gene (C11orf10) located immediately upstream of the FEN1 gene in reverse orientation. Immunostaining revealed that the C11orf10 protein, unlike FEN-1, is located in the cytoplasm, suggesting that these two proteins do not form a physical complex. Importantly, in the human genome, the two mRNAs are overlapping (14 bp) in their 5' ends. Thus, the FEN1/C11orf10 locus is a new example of two overlapping, divergent transcription units in the human genome.
Asunto(s)
Endodesoxirribonucleasas/genética , Genoma Humano , Transcripción Genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Línea Celular , Núcleo Celular/enzimología , Pollos , Clonación Molecular , ADN Complementario/metabolismo , Células Epiteliales/citología , Endonucleasas de ADN Solapado , Humanos , Ratones , Datos de Secuencia Molecular , ARN Mensajero/metabolismo , Homología de Secuencia de AminoácidoRESUMEN
The structure-specific FEN-1 endonuclease has been implicated in various cellular processes, including DNA replication, repair and recombination. In vertebrate cells, however, no in vivo evidence has been provided so far. Here, we knocked out the FEN-1 gene (FEN1) in the chicken DT40 cell line. Surprisingly, homozygous mutant (FEN1-/-) cells were viable, indicating that FEN-1 is not essential for cell proliferation and thus for Okazaki fragment processing during DNA replication. However, compared with wild-type cells, FEN1-/- cells exhibited a slow growth phenotype, probably due to a high rate of cell death. The mutant cells were hypersensitive to methylmethane sulfonate, N-methyl-N'-nitro-N-nitrosoguanidine and H2O2, but not to UV light, X-rays and etoposide, suggesting that FEN-1 functions in base excision repair in vertebrate cells.