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OBJECTIVE: To analyze the factors associated with the indication of echocardiography for the investigation of congenital heart disease among newborns. METHOD: Retrospective sectional study through the collection of 848 medical records of patients admitted to maternity hospitals in Rio de Janeiro-Brazil, respecting the time frame from September to December 2022. RESULTS: The average age of mothers was 26.5±6.3 years; 52.7% were classified as brown. The average age of the newborns was 3.5±5.6 days. Maternal variables: gestational age (OR=6.93, CI:3.76-12.80), number of gestational risk factors (1.90: 1.47-2.45) and number of medications (1. 97: 1.40-2.77); and neonatal variables: age (1.07: 1.03-1.02), prematurity (10.55: 5.29-21.03) and number of risk factors (2.62: 2.03-3 .38) were significantly associated with the indication for echocardiography (p<0.001). CONCLUSION: It is concluded that the different maternal and neonatal variables, gestational age, number of gestational risk factors, number of medications, age, prematurity and number of risk factors, respectively, showed a significant association for the indication of echocardiography. Therefore, the identification of these factors will enable the investigation of congenital heart disease at an opportune time among newborns.

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Rhenium complexes show great promise as anticancer drug candidates. Specifically, compounds with a Re(CO)3(NN)(py)+ core in their architecture have shown cytotoxicity equal to or greater than that of well-established anticancer drugs based on platinum or organic molecules. This study aimed to evaluate how the strength of the interaction between rhenium(I) tricarbonyl complexes fac-[Re(CO)3(NN)(py)]+, NN = 1,10-phenanthroline (phen), dipyrido[3,2-f:2',3'-h]quinoxaline (dpq) or dipyrido[3,2-a:2'3'-c]phenazine (dppz) and biomolecules (protein, lipid and DNA) impacted the corresponding cytotoxic effect in cells. Results showed that fac-[Re(CO)3(dppz)(py)]+ has higher Log Po/w and binding constant (Kb) with biomolecules (protein, lipid and DNA) compared to complexes of fac-[Re(CO)3(phen)(py)]+ and fac-[Re(CO)3(dpq)(py)]+. As consequence, fac-[Re(CO)3(dppz)(py)]+ exhibited the highest cytotoxicity (IC50 = 8.5 µM for HeLa cells) for fac-[Re(CO)3(dppz)(py)]+ among the studied compounds (IC50 > 15 µM). This highest cytotoxicity of fac-[Re(CO)3(dppz)(py)]+ are probably related to its lipophilicity, higher permeation of the lipid bilayers of cells, and a more potent interaction of the dppz ligand with biomolecules (protein and DNA). Our findings open novel avenues for rational drug design and highlight the importance of considering the chemical structures of rhenium complexes that strongly interact with biomolecules (proteins, lipids, and DNA).

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RESUMO Este estudo objetivou analisar os fatores contextuais do desempenho do atendimento odontológico para gestantes na Atenção Básica entre municípios baianos. A série temporal utilizou dados secundários dos 417 municípios, entre 2018 e 2022. O desfecho foi a meta do indicador de proporção de gestantes com atendimento odontológico por município: insuficiente (<60,0%) ou suficiente (≥60,0%). As variáveis contextuais foram: fatores sociodemográficos (Porte Populacional, Índice de Desenvolvimento Humano Municipal, Produto Interno Bruto municipal per capita - PIB, Índice de Gini da renda domiciliar per capita, Índice de Vulnerabilidade Social e Núcleos Regionais de Saúde) e serviço de saúde (Cobertura de Estratégia Saúde da Família - ESF e de Saúde Bucal). Realizou-se regressão de Poisson uni e multivariada (p<0,05). No último quadrimestre de cada ano: 2,9% (2018), 2,4% (2019), 5,3% (2020), 37,2% (2021) e 71,2% (2022) dos municípios atingiram a meta. No terceiro quadrimestre de 2021, os municípios com menor PIB, e em 2021 e 2022, com maior cobertura de ESF, apresentaram maior chance de atingir a meta. Conclui-se que houve aumento da proporção de atendimento odontológico para gestante ao longo dos anos entre os municípios baianos e atingir a meta foi associado a fatores sociodemográficos e de serviço de saúde.

ABSTRACT This study aimed to analyze the contextual factors of the performance of dental care for pregnant women in Primary Care among municipalities in Bahia. The time series used secondary data from 417 municipalities between 2018-2022. The outcome was the target indicator for the proportion of pregnant women with dental care per municipality: insufficient (<60.0%) or sufficient (≥60.0%). The contextual variables were: sociodemographic factors (Population Size, Municipal Human Development Index, municipal Gross Domestic Product per capita - GDP, Gini Index of household income per capita, Social Vulnerability Index and Regional Health Centers) and health service (Coverage of Family Health Strategy - ESF and Oral Health). Poisson regression was performed (p<0.05). In the last four months of each year: 2.9% (2018), 2.4% (2019), 5.3% (2020), 37.2% (2021) and 71.2% (2022) of the municipalities reached the goal. In the third four months of 2021, municipalities with lower GDP, and in 2021 and 2022, with greater ESF coverage, had a greater chance of reaching the target. It is concluded that there was an increase in the proportion of dental care for pregnant women over the years among the municipalities in Bahia, and achieving the goal was associated with sociodemographic and health service factors.

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ABSTRACT Objective: To analyze the factors associated with the indication of echocardiography for the investigation of congenital heart disease among newborns. Method: Retrospective sectional study through the collection of 848 medical records of patients admitted to maternity hospitals in Rio de Janeiro-Brazil, respecting the time frame from September to December 2022. Results: The average age of mothers was 26.5±6.3 years; 52.7% were classified as brown. The average age of the newborns was 3.5±5.6 days. Maternal variables: gestational age (OR=6.93, CI:3.76-12.80), number of gestational risk factors (1.90: 1.47-2.45) and number of medications (1. 97: 1.40-2.77); and neonatal variables: age (1.07: 1.03-1.02), prematurity (10.55: 5.29-21.03) and number of risk factors (2.62: 2.03-3 .38) were significantly associated with the indication for echocardiography (p<0.001). Conclusion: It is concluded that the different maternal and neonatal variables, gestational age, number of gestational risk factors, number of medications, age, prematurity and number of risk factors, respectively, showed a significant association for the indication of echocardiography. Therefore, the identification of these factors will enable the investigation of congenital heart disease at an opportune time among newborns.

RESUMEN Objetivo: Analizar los factores asociados a la indicación de la ecocardiografía para la investigación de cardiopatías congénitas en recién nacidos. Método: Estudio seccional retrospectivo a través de la recolección de 848 historias clínicas de pacientes ingresadas en maternidades de Río de Janeiro-Brasil, respetando el período de septiembre a diciembre de 2022. Cálculos del odds ratio, intervalo de confianza del 95% en el nivel de significancia de 5%. Resultados: La edad promedio de las madres fue de 26,5±6,3 años; El 52,7% fueron clasificados como pardos. La edad promedio de los recién nacidos fue de 3,5±5,6 días. Variables maternas: edad gestacional (OR=6,93, IC:3,76-12,80), número de factores de riesgo gestacional (1,90: 1,47-2,45) y número de medicamentos (1,97: 1,40-2,77); y variables neonatales: edad (1,07: 1,03-1,02), prematuridad (10,55: 5,29-21,03) y número de factores de riesgo (2,62: 2,03-3,38) se asociaron significativamente con la indicación de ecocardiografía (p<0,001). Conclusión: Se concluye que las diferentes variables maternas y neonatales, edad gestacional, número de factores de riesgo gestacional, número de medicamentos, edad, prematuridad y número de factores de riesgo, respectivamente mostraron asociación significativa para la indicación de ecocardiografía. Por lo tanto, la identificación de estos factores permitirá investigar las cardiopatías congénitas en un momento oportuno entre los recién nacidos.

RESUMO Objetivo: Analisar os fatores associados à indicação de ecocardiografia para a investigação de cardiopatia congênita entre recém-nascidos. Método: Estudo seccional retrospectivo por meio da coleta em 848 prontuários de pacientes internados em maternidades no Rio de Janeiro-Brasil, respeitando o recorte temporal de setembro a dezembro de 2022. Realizados cálculos da razão de chance, intervalo de confiança de 95% em nível de significância de 5%. Resultados: A média de idade das mães foi de 26,5±6,3 anos; 52,7% foram classificadas como pardas. A idade média dos recém-nascidos foi de 3,5±5,6 dias.As variáveis maternas: idade gestacional (RC=6,93, IC:3,76-12,80), número de fatores de risco gestacional (1,90: 1,47-2,45) e número de medicamentos (1,97: 1,40-2,77); e as variáveis neonatais: idade (1,07: 1,03-1,02), prematuridade (10,55: 5,29-21,03) e número de fatores de risco (2,62: 2,03-3,38) se associaram significativamente à indicação de ecocardiografia (p<0,001). Conclusão: Conclui-se que as distintas variáveis materna e neonatal,idade gestacional, número de fatores de risco gestacional número de medicamentos, idade, prematuridade e números de fatores de risco, respectivamente apresentaram associação significativa para a indicação da ecocardiografia. Logo, a identificação desses fatores possibilitará a investigação de cardiopatia congênita em momento oportuno entre os recém-nascidos.

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A new species of Myxobolus parasitizing the arterial bulb and cardiac musculature of the freshwater fish Pimelodus ornatus Kner, 1858, from the Arari river in the municipality of Cachoeira do Arari, island of Marajó, Pará, Brazil, was described. In the present study, the observed prevalence of myxozoan parasites in the heart tissue of the hosts was 20% (6/30). The myxozoans observed had mature biconvex spores, slightly rounded, an anterior end with two pyriform polar capsules and a posterior end with very evident sporoplasm, measuring 8 ± 0.2 µmin length. The spore width was 5.8 ± 0.4 µm, with a thickness of 3.4 ± 0.2µm. The length of the polar capsules was 3.6 ± 0.3 µm and the width was 1.2 ± 0.2µm, with 6 to 7 turns of the polar filament. The divergences observed, regarding the morphometric and genetic structure of SSU rDNA, in relation to other Myxobolidae already described in the literature, confirm the description of the new species Myxobolus rangeli n. sp.

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Introdução:A Atenção Primária à Saúde é caracterizada como a porta de entrada preferencial do Sistema Único de Saúde, ocupando lugar central no serviço. Todavia, a pandemia da COVID-19 instigou a necessidade de mudanças naorganização do trabalho das equipes de saúde da atenção básica.Objetivo:Neste cenário, torna-se fundamental conhecer a atuação docirurgião-dentista da Atenção Primária à Saúdeno que se refere à oferta dos serviços odontológicos, alterações no processode trabalho e capacidade técnica de ação, frente às condições estabelecidas pela pandemiadaCOVID-19.Metodologia:Trata-se de um estudo de caráter transversal descritivo e abordagem quantitativa, desenvolvido em um município do interior baiano, com 29 cirurgiões-dentistas vinculados àatenção básica. Esses profissionais foram convidados a responder um questionário on-lineque abordou questões relativas ao desempenho dos cirurgiões-dentistas e a condução das ações de saúde bucal frente às medidas restritivas impostas pela pandemiada COVID-19.Resultados:Foi possível identificar que o processo de trabalho e acesso da população aos serviços de odontologia sofreram interferências, expressas através da limitação do acesso da população à saúde bucal, alterações nas técnicas terapêuticas e interrupção das ações em saúde de abrangência coletiva.Conclusões:O presente estudo apontou limitaçõesno processo de trabalho do cirurgião-dentista da Atenção Primária à Saúdedurante a pandemia da COVID-19, como a dificuldade para atuar em equipe interprofissional e atender as proposições estabelecidas pelaPolítica Nacional da Atenção Básica (AU).

Introduction:Primary Health Care is the preferred gateway to the Brazilian Unified Health System, occupying a central place in the service. However, the COVID-19 pandemic instigated the need for changes in the organization of work of primary care health teams.Objective:In this scenario, it is essential to know the performance of dental surgeons in terms ofdental care offer, changes in the work process and of their technical capacity for action, in the face of the conditions established by the pandemic.Methodology:This is a descriptive cross-sectional study with a quantitative approach developed in a city in the countryside of Bahia with 29 dental surgeons linked to primary care. These professionals were invited to answer an online questionnaire that addressed issues related to the performance of dental surgeons and the management of oral health actions in the face of the restrictive measures imposed by the COVID-19 pandemic.Results:It was possible to identify that the pandemic interfered with the work process and the population's access to dental services, expressed through the limitation of the population's access to oral health, changes in therapeutic techniques and interruption of collective health actions.Conclusions:The present study pointed out limitations in the work process of the primary care dentist during the COVID-19 pandemic, such as the difficulty in working in an interprofessional team and meeting the propositions established by the Brazilian National Primary Care Policy in Health (AU).

Introducción: La Atención Primaria de Salud se caracteriza por ser la puerta de entrada preferente al Sistema Único de SaludBrasileño, ocupando un lugar central en el servicio. Sin embargo, la pandemia de COVID-19 instigó la necesidad de cambios en la organización del trabajo de los equipos de salud de atención primaria.Objetivo: En este escenario, es fundamental conocer el desempeño del odontólogo en cuanto, oferta de cuidado dental, cambios enel procesode trabajo, ya su capacidad técnica de actuación, ante las condiciones establecidas por la pandemiaMetodología: Se trata de un estudio descriptivo transversal con enfoque cuantitativo, desarrollado en una ciudad del interior de Bahía, con 29 odontólogos vinculados a la atención primaria. Estos profesionales fueron invitados a responder un cuestionario en línea que abordaba cuestiones relacionadas con la actuación de los odontólogos y la realización de acciones de salud bucal frente a las medidas restrictivas impuestas por la pandemia de la COVID-19.Resultados: Con los resultados fue posible identificar que el proceso de trabajo y el acceso de la población a los servicios odontológicos sufrieron interferencias, expresadas a través de la limitación del acceso de la población a la salud bucal, cambios en las técnicas terapéuticas e interrupción de las acciones de salud colectiva. Conclusiones: El presente estudio señaló limitaciones en el proceso de trabajo del cirujano dentista de la atención en lasaluddurante la pandemia de COVID-19, como la dificultad para trabajar enequipo interprofesional y cumplir con las proposiciones establecidas por la Política Brasileña de Atención Primaria en Salud (AU).

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A new species of Myxobolus parasitizing the arterial bulb and cardiac musculature of the freshwater fish Pimelodus ornatus Kner, 1858, from the Arari river in the municipality of Cachoeira do Arari, island of Marajó, Pará, Brazil, was described. In the present study, the observed prevalence of myxozoan parasites in the heart tissue of the hosts was 20% (6/30). The myxozoans observed had mature biconvex spores, slightly rounded, an anterior end with two pyriform polar capsules and a posterior end with very evident sporoplasm, measuring 8 ± 0.2 μmin length. The spore width was 5.8 ± 0.4 μm, with a thickness of 3.4 ± 0.2μm. The length of the polar capsules was 3.6 ± 0.3 μm and the width was 1.2 ± 0.2μm, with 6 to 7 turns of the polar filament. The divergences observed, regarding the morphometric and genetic structure of SSU rDNA, in relation to other Myxobolidae already described in the literature, confirm the description of the new species Myxobolus rangeli n. sp.(AU)

Descrição de uma nova espécie de Myxobolus que parasita o bulbo arterial e a musculatura cardíaca do peixe de água doce Pimelodus ornatus Kner, 1858, do rio Arari, no município de Cachoeira do Arari, ilha de Marajó, Pará, Brasil. No presente estudo, a prevalência observada de parasitas mixozoários no tecido cardíaco dos hospedeiros foi de 20% (6/30). Os mixozoários observados apresentavam esporos maduros biconvexos, levemente arredondados, extremidade anterior com duas cápsulas polares piriformes e extremidade posterior com esporoplasma bem evidente, medindo 8 ± 0,2 μm de comprimento. A largura do esporo foi de 5,8 ± 0,4 μm, com espessura de 3,4 ± 0,2 μm. O comprimento das cápsulas polares foi de 3,6 ± 0,3 μm e a largura foi de 1,2 ± 0,2 μm, com 6 a 7 voltas do filamento polar. As divergências observadas, quanto à estrutura morfométrica e genética de SSU rDNA, em relação a outros Myxobolidae já descritos na literatura, confirmam a descrição da nova espécie Myxobolus rangeli n. sp.(AU)

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Light-based therapies and diagnoses including photodynamic therapy (PDT) have been used in many fields of medicine, including the treatment of non-oncological diseases and many types of cancer. PDT require a light source and a light-sensitive compound, called photosensitizer (PS), to detect and destroy cancer cells. After absorption of the photon, PS molecule gets excited from its singlet ground state to a higher electronically excited state which, among several photophysical processes, can emit light (fluorescence) and/or generate reactive oxygen species (ROS). Moreover, the biological responses are activated only in specific areas of the tissue that have been submitted to exposure to light. The success of the PDT depends on many parameters, such as deep light penetration on tissue, higher PS uptake by undesired cells as well as its photophysical and photochemical characteristics. One of the challenges of PDT is the depth of penetration of light into biological tissues. Because photon absorption and scattering occur simultaneously, these processes depend directly on the light wavelength. Using PS that absorbs photons on "optical transparency windows" of biological tissues promises deeper penetration and less attenuation during the irradiation process. The traditional PS normally is excited by a higher energy photon (UV-Vis light) which has become the Achilles' heel in photodiagnosis and phototreatment of deep-seated tumors below the skin. Thus, the need to have an effective upconverter sensitizer agent is the property in which it absorbs light in the near-infrared (NIR) region and emits in the visible and NIR spectral regions. The red emission can contribute to the therapy and the green and NIR emission to obtain the image, for example. The absorption of NIR light by the material is very interesting because it allows greater penetration depth for in vivo bioimaging and can efficiently suppress autofluorescence and light scattering. Consequently, the penetration of NIR radiation is greater, activating the biophotoluminescent material within the cell. Thus, materials containing Rare Earth (RE) elements have a great advantage for these applications due to their attractive optical and physicochemical properties, such as several possibilities of excitation wavelengths - from UV to NIR, strong photoluminescence emissions, relatively long luminescence decay lifetimes (µs to ms), and high sensitivity and easy preparation. In resume, the relentless search for new systems continues. The contribution and understanding of the mechanisms of the various physicochemical properties presented by this system is critical to finding a suitable system for cancer treatment via PDT.

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Kudoa ocellatus n. sp. was found in the musculature of Astronotus ocelattus (Agassiz, 1831) from the Arari River on Marajó Island in Pará, Brazil. The new species forms pseudocysts in the epaxial and hypaxial musculature composed of various spores that are pseudoquadrate in the apical view. In the lateral view, the spores were triangular or pyramidal. In the lateral view, the spores were 46 ± 0.11 µm (4.5-4.8) in length and 6.6 ± 0.3 µm (6.2-7.2) in width, with four pyriform polar capsules of equal size that measured 2.0 ± 0.16 µm (1.8-2.2) in length and 1.5 ± 0.18 µm (1.3-1.8) in width. Based on the partial (1418 bps) sequence of the SSU rDNA gene, Kudoa ocellatus n. sp. was distinct from all the other Kudoa species deposited in GenBank. The phylogenetic Bayesian Inference and P distance placed the new species together with the other Kudoa species that parasitize freshwater Amazonian fish. The morphological evidence, together with the SSU rDNA gene sequence, supported the description of Kudoa ocellatus n. sp., a distinct new species of the genus, which parasitizes a freshwater Amazonian cichlid.

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O objetivo desse estudofoi verificar a contaminação microbiológica deresinas compostas utilizadas em uma clínica-escola de Odontologia. Trata-se de uma pesquisa experimental/laboratorial, na qual foramcoletadas amostras de 10 bisnagas de resina composta, sendo uma delas o controle negativo. Porções de resina composta contidas no interior das bisnagas foram coletadas e mergulhadas em tubos de ensaio contendo caldo nutriente eposterior semeadura em placas e coloraçãopara caracterizar as colônias e observar bactérias e fungos. Todas as amostras apresentaram contaminação, inclusive o tubo contendo meio de cultura utilizado como controle de manuseio do experimento. Essas contaminações podem ter relação com as falhas dos meios de biossegurança empregados na clínica-escola e com os métodos de transporte e armazenamento das bisnagas de resina composta. Portanto, existe a necessidade de conscientização dos estudantese docentespara a adoção de medidas de biossegurança específicas para o manuseio das resinas compostas (AU).

The aim of this study was to verify the microbiological contamination in composite resins used at one Dental school clinic. This is an experimental/laboratory study, in which samples of 10 tubes of composite resin were collected, one of which was the negative control. Portions of composite resin inside the tubes were collected and dipped in test tubes containing nutrient broth for subsequent seeding on to plates and staining for the characterization of bacterial and fungal colonies. All samples revealed contamination, including the tube containing culture medium used as control for handling the experiment. These contaminations may be related to failures in biosafety measures employed in theDental school clinic and to the transport and storage methods for the tubes of composite resin. Therefore, it is necessary to raise awareness among students and teachers to adopt specific biosafety measures for the handling of composite resins (AU).

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A total of 30 specimens of the Amazonian electric knifefish, Brachyhypopomus beebei Schultz, 1944 (Gymnotiformes: Hypopomidae), were collected from the Peixe-Boi River in the state of Pará, Brazil (1°06'59" S; 47°18'26" W). Fragments of the brain tissue were extracted for analysis via optical microscopy, and 18 specimens (60%) presented microparasites of the genus Myxobolus, with unequal capsules. The spores were 18.6 µm (17.7-19.8 µm) long and 8.6 µm (8.4-9.0 µm) wide; the largest polar capsule was 13.0 µm (12.4-13.4 µm) long and 5.6 µm (5.3-6.0 µm) wide, and the smallest capsule was 5.0 µm (4.5-5.3 µm) long and 2.5 µm (2.3-2.6 µm) wide. Infected brain fragments were extracted for histological processing and staining with hematoxylin-eosin and Ziehl-Neelsen. Some fragments were conserved in ethanol for molecular genetics analysis. A partial sequence of the 18S DNA gene was obtained from the spores, which did not correspond to any other sequences deposited in GenBank, although it did form a clade with other Myxobolus parasites of the nervous system. The morphological data, together with molecular phylogeny, supported the designation of a new species Myxobolus freitasi n. sp.

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Trypanosoma cruzi is the agent of Chagas disease, an infection that affects around 8 million people worldwide. The search for new anti-T. cruzi drugs are relevant, mainly because the treatment of this disease is limited to two drugs. The objective of this study was to investigate the trypanocidal and cytotoxic activity and elucidate the chemical profile of extracts from the roots of the Lonchocarpus cultratus. Roots from L. cultratus were submitted to successive extractions with hexane, dichloromethane, and methanol, resulting in LCH, LCD, and LCM extracts, respectively. Characterization of extracts was done using 1H-RMN, 13C-RMN, CC and TLC. Treatment of T. cruzi forms (epimastigotes, trypomastigotes, and amastigotes) with crescent concentrations of LCH, LCD, and LCM was done for 72, 48, and 48 h, respectively. After this, the percentage of inhibition and IC50/LC50 were calculated. Benznidazole was used as a positive control. Murine macrophages were treated with different concentrations of both extracts for 48 h, and after, the cellular viability was determined by the MTT method and CC50 was calculated. The chalcones derricin and lonchocarpine were identified in the hexane extract, and for the first time in the genus Lonchocarpus, the presence of a dihydrolonchocarpine derivative was observed. Other chalcones such as isocordoin and erioschalcone B were detected in the dichloromethane extract. The dichloromethane extract showed higher activity against all tested forms of T. cruzi than the other two extracts, with IC50 values of 10.98, 2.42, and 0.83 µg/mL, respectively; these values are very close to those of benznidazole. Although the dichloromethane extract presented a cytotoxic effect against mammalian cells, it showed selectivity against amastigotes. The methanolic extract showed the lowest anti-T. cruzi activity but was non-toxic to peritoneal murine macrophages. Thus, the genus Lonchocarpus had demonstrated in the past action against epimastigotes forms of T. cruzi but is the first time that the activity against infective forms is showed, which leading to further studies with in vivo tests.

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A total of 30 specimens of the Amazonian electric knifefish, Brachyhypopomus beebei Schultz, 1944 (Gymnotiformes: Hypopomidae), were collected from the Peixe-Boi River in the state of Pará, Brazil (1°0659 S; 47°1826 W). Fragments of the brain tissue were extracted for analysis via optical microscopy, and 18 specimens (60%) presented microparasites of the genus Myxobolus, with unequal capsules. The spores were 18.6 µm (17.7-19.8 µm) long and 8.6 µm (8.4-9.0 µm) wide; the largest polar capsule was 13.0 µm (12.4-13.4 µm) long and 5.6 µm (5.3-6.0 µm) wide, and the smallest capsule was 5.0 µm (4.5-5.3 µm) long and 2.5 µm (2.3-2.6 µm) wide. Infected brain fragments were extracted for histological processing and staining with hematoxylin-eosin and Ziehl-Neelsen. Some fragments were conserved in ethanol for molecular genetics analysis. A partial sequence of the 18S DNA gene was obtained from the spores, which did not correspond to any other sequences deposited in GenBank, although it did form a clade with other Myxobolus parasites of the nervous system. The morphological data, together with molecular phylogeny, supported the designation of a new species Myxobolus freitasi n. sp.(AU)

Um total de 30 espécimes do peixe-faca elétrico da Amazônia, Brachyhypopomus beebei Schultz, 1944 (Gymnotiformes: Hypopomidae), foram coletados no rio Peixe-Mani, no estado do Pará, Brasil (1 ° 06'59 S; 47 ° 18 ' 26 W). Fragmentos de tecido cerebral foram extraídos para análise em microscopia óptica, sendo que 18 espécimes (60%) apresentavam microparasitos do gênero Myxobolus, com cápsulas desiguais. Os esporos apresentavam 18,6 µm (17,7-19,8 µm) de comprimento e 8,6 µm (8,4-9,0 µm) de largura; a maior cápsula polar tinha 13,0 µm (12,4-13,4 µm) de comprimento e 5,6 µm (5,3-6,0 µm) de largura, e a menor cápsula tinha 5,0 µm (4,5-5,3 µm) de comprimento e 2,5 µm (2,3-2,6 µm) de largura. Fragmentos cerebrais infectados foram extraídos para processamento histológico e coloração com hematoxilina-eosina e Ziehl-Neelsen. Alguns fragmentos foram conservados em etanol para análise genética molecular. Dos esporos, foi obtida uma sequência parcial do gene 18S do DNA, que não correspondeu a nenhuma outra sequência depositada no GenBank, embora tenha formado um clado com outros parasitas do gênero Myxobolus do sistema nervoso. Os dados morfológicos, juntamente com a filogenia molecular, apoiaram a designação de uma nova espécie Myxobolus freitasi n. sp.(AU)

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Abstract A total of 30 specimens of the Amazonian electric knifefish, Brachyhypopomus beebei Schultz, 1944 (Gymnotiformes: Hypopomidae), were collected from the Peixe-Boi River in the state of Pará, Brazil (1°06'59" S; 47°18'26" W). Fragments of the brain tissue were extracted for analysis via optical microscopy, and 18 specimens (60%) presented microparasites of the genus Myxobolus, with unequal capsules. The spores were 18.6 µm (17.7-19.8 µm) long and 8.6 µm (8.4-9.0 µm) wide; the largest polar capsule was 13.0 µm (12.4-13.4 µm) long and 5.6 µm (5.3-6.0 µm) wide, and the smallest capsule was 5.0 µm (4.5-5.3 µm) long and 2.5 µm (2.3-2.6 µm) wide. Infected brain fragments were extracted for histological processing and staining with hematoxylin-eosin and Ziehl-Neelsen. Some fragments were conserved in ethanol for molecular genetics analysis. A partial sequence of the 18S DNA gene was obtained from the spores, which did not correspond to any other sequences deposited in GenBank, although it did form a clade with other Myxobolus parasites of the nervous system. The morphological data, together with molecular phylogeny, supported the designation of a new species Myxobolus freitasi n. sp.

Resumo Um total de 30 espécimes do peixe-faca elétrico da Amazônia, Brachyhypopomus beebei Schultz, 1944 (Gymnotiformes: Hypopomidae), foram coletados no rio Peixe-Mani, no estado do Pará, Brasil (1 ° 06'59 "S; 47 ° 18 ' 26 "W). Fragmentos de tecido cerebral foram extraídos para análise em microscopia óptica, sendo que 18 espécimes (60%) apresentavam microparasitos do gênero Myxobolus, com cápsulas desiguais. Os esporos apresentavam 18,6 µm (17,7-19,8 µm) de comprimento e 8,6 µm (8,4-9,0 µm) de largura; a maior cápsula polar tinha 13,0 µm (12,4-13,4 µm) de comprimento e 5,6 µm (5,3-6,0 µm) de largura, e a menor cápsula tinha 5,0 µm (4,5-5,3 µm) de comprimento e 2,5 µm (2,3-2,6 µm) de largura. Fragmentos cerebrais infectados foram extraídos para processamento histológico e coloração com hematoxilina-eosina e Ziehl-Neelsen. Alguns fragmentos foram conservados em etanol para análise genética molecular. Dos esporos, foi obtida uma sequência parcial do gene 18S do DNA, que não correspondeu a nenhuma outra sequência depositada no GenBank, embora tenha formado um clado com outros parasitas do gênero Myxobolus do sistema nervoso. Os dados morfológicos, juntamente com a filogenia molecular, apoiaram a designação de uma nova espécie Myxobolus freitasi n. sp.

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Pesquisa foi motivada a investigar como as mulheres trabalhadoras realizava a manutenção do aleitamento materno ao retornar de seu trabalho, que teve como objetivo descrever se havia dificuldades e estratégias realizadas para a manutenção do aleitamento. O método análise dos dados pelo referencial de Bardin, realizada entre os meses de setembro à outubro de 2017, com vinte mulheres entre 19 a 39 anos de idade cadastradas e atendidas na unidade de estratégia saúde da família do município do Rio de Janeiro, sendo produzidas duas categorias: Paradoxo da rede de apoio; Ausência do Enfermeiro no trabalho multiprofissional. Resultado obteve como desmame precoce havendo a introdução de leite pasteurizado como consequência. Conclui-se que há insegurança para a realização de ações de continuidade de aleitamento materno após o retorno ao trabalho.(AU)

The research was motivated to investigate the hard-working women accomplished the maintenance of the maternal breast feeding when returning of his / her work, that he / she had an objective described if there were difficulties and strategies accomplished for the maintenance of the breast feeding. The methodology analysis of the data for the reference of Bardin, conducted between the months of September to October of 2017, twenty women among 19 to 39 years of age registered and assisted in the unit of strategy. municipal district of Rio de Janeiro, being produced two categories: Paradox of the support net; Absence of the Nurse in the multi-professional work. Result obtained how it weans precocious having the introduction of milk pasteurized as consequence. It is ended that there is insecurity for the accomplishment of actions of continuity of maternal breast feeding after the return to the work.(AU)

La investigación fue motivada a investigar cómo las mujeres trabajadoras realizaban el mantenimiento de la lactancia materna al retornar de su trabajo, que tuvo como objetivo describir si había dificultades y estrategias realizadas para el mantenimiento de la lactancia. La metodología análisis de los datos por el referencial de Bardin, realizada entre los meses de septiembre a octubre de 2017, veinte mujeres entre 19 a 39 años de edad registradas y atendidas en la unidad de estrategia salud de la familia del municipio de Río de Janeiro En enero, se producirá dos categorías: Paradoja de la red de apoyo; Ausencia del enfermero en el trabajo multiprofesional. Resultado obtenido como destete precoz habiendo la introducción de leche pasteurizada como consecuencia. Se concluye que hay inseguridad para la realización de acciones de continuidad de lactancia materna después del retorno al trabajo.(AU)

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O objetivo do presente trabalho foi avaliar a influência da concentração da maltodextrina e da temperatura de entrada de secagem sob as características físico-químicas de polpa de acerola em pó. As acerolas maduras foram selecionadas, lavadas, sanitizadas, enxaguadas e despolpada. As polpas foram divididas em quatro tratamentos, utilizando-se duas concentrações de maltodextrina (14 e 26%) que foram atomizadas em spray dryer em duas temperaturas (128 e 152°C). A polpa em pó e in natura foram analisadas quanto a umidade, SST, ATT, pH e carotenoides. De acordo com os resultados obtidos foi possível verificar que os teores de umidade foram influenciados pela temperatura e concentração de maltodextrina. A acidez da polpa em pó diminui com o aumento da do agente carreador. O teor de carotenoides não apresentou diferença significativa entre os tratamentos estudados. Para o processo de atomização da acerola por spray dryer indica-se o uso de maltodextrina a 26% independente da temperatura.(AU)

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O objetivo do presente trabalho foi obter e caracterizar de polpa de cajá-umbu atomizada por spray-dryer. A cajá-umbu madura passaram pelas etapas de seleção, lavagem, sanitização, enxágue e despolpamento, obtendo-se assim a polpa. Para atomização por spray dryer adicionou-se 15% de maltodextrina e atomizou em três temperaturas de entrada (100, 120 e 160ºC). A polpa de cajá-umbu in natura e atomizadas foram submetidas a análise de umidade, atividade de água (aw), cinzas, sólidos solúveis, acidez, pH, vitamina C e carotenoides. A polpa in natura apresentou maiores teores de carotenoides (16,58 µg·g-1) demonstrando que a atomização influência nos carotenoides, já a polpa atomizada, apresentou menor teor de umidade com o aumento da temperatura de secagem e reduziu a aw. Para obtenção do pó de cajá-umbu indica-se o uso da temperatura de 120°C.(AU)

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O objetivo do presente trabalho foi obter e caracterizar de polpa de cajá-umbu atomizada por spray-dryer. A cajá-umbu madura passaram pelas etapas de seleção, lavagem, sanitização, enxágue e despolpamento, obtendo-se assim a polpa. Para atomização por spray dryer adicionou-se 15% de maltodextrina e atomizou em três temperaturas de entrada (100, 120 e 160ºC). A polpa de cajá-umbu in natura e atomizadas foram submetidas a análise de umidade, atividade de água (aw), cinzas, sólidos solúveis, acidez, pH, vitamina C e carotenoides. A polpa in natura apresentou maiores teores de carotenoides (16,58 µg·g-1) demonstrando que a atomização influência nos carotenoides, já a polpa atomizada, apresentou menor teor de umidade com o aumento da temperatura de secagem e reduziu a aw. Para obtenção do pó de cajá-umbu indica-se o uso da temperatura de 120°C.

Asunto(s)

RESUMEN

O objetivo do presente trabalho foi avaliar a influência da concentração da maltodextrina e da temperatura de entrada de secagem sob as características físico-químicas de polpa de acerola em pó. As acerolas maduras foram selecionadas, lavadas, sanitizadas, enxaguadas e despolpada. As polpas foram divididas em quatro tratamentos, utilizando-se duas concentrações de maltodextrina (14 e 26%) que foram atomizadas em spray dryer em duas temperaturas (128 e 152°C). A polpa em pó e in natura foram analisadas quanto a umidade, SST, ATT, pH e carotenoides. De acordo com os resultados obtidos foi possível verificar que os teores de umidade foram influenciados pela temperatura e concentração de maltodextrina. A acidez da polpa em pó diminui com o aumento da do agente carreador. O teor de carotenoides não apresentou diferença significativa entre os tratamentos estudados. Para o processo de atomização da acerola por spray dryer indica-se o uso de maltodextrina a 26% independente da temperatura.

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Manipulation of implant surface characteristics constitutes a promising strategy for improving cell growth and tissue response on a variety of materials with different surface topographies. Mesenchymal progenitor cells with a capacity to respond to titanium surface stimuli and differentiate into osteoblasts were used to perform comparative tests between two different implant topographies, including their functional interaction with pre-osteoblasts directly seeded onto the implants. Functional analysis of nanostructured implant surfaces was performed by in vitro assay analysis. The machined surface of titanium implants (mach group) was used as a control and compared with a nanoparticle HA activated surface implant (nano group), developed by the deposition of pure crystalline hydroxyapatite. Cell culture on the nano group surface resulted in higher cell adhesion and cultured osteoblast viability compared with the mach group. Scanning electron microscope (SEM) images revealed a stable interaction, indicated by the presence of focal cell adhesion formation. These results together with positive mineralization assays showed the nano group to be an excellent scaffold for bone-implant integration.