RESUMEN
Twenty-month-old Swiss mice were allocated into three groups: (A) control; (B) infected group; and (C) infected but treated with 5 mg of the phytocompound MMT. Mice were infected intranasally with 30 microL of 75 HA viral units. MMT markedly blunted the nasal signs of virus infection and the febrile response. Formazan-positive cells, lung and plasma lipoperoxides, and TNF-alpha in lung tissue increased during viral infection, but improvement was seen in the MMT-treated group (P < 0.05). MMT also normalized SOD, catalase activities, and ascorbic acid and determined a significant decrease of lung but not nasal viral titer, although nasal inflammatory infiltrate dropped significantly. MMT has potential clinical applications with and has an excellent safety profile even in old animals.
Asunto(s)
Envejecimiento/metabolismo , Antioxidantes/administración & dosificación , Suplementos Dietéticos , Infecciones por Orthomyxoviridae/dietoterapia , Administración Oral , Animales , Ácido Ascórbico/análisis , Líquido del Lavado Bronquioalveolar/química , Catalasa/análisis , Quimiocina CCL5/análisis , Modelos Animales de Enfermedad , Esquema de Medicación , Pulmón/enzimología , Pulmón/metabolismo , Pulmón/virología , Ratones , Infecciones por Orthomyxoviridae/virología , Distribución Aleatoria , Superóxido Dismutasa/análisis , Resultado del Tratamiento , Factor de Necrosis Tumoral alfa/análisis , Carga ViralRESUMEN
Hepatocytes isolated from 20- and 4-month Wistar rats and cultured with or without alpha-linolenic acid (LNA) were then added with nutraceutical YHK or sylibin before the test with iron or copper. Overall, YHK proved to be more effective than sylibin in Fe/Cu-induced peroxidative damage on normal and LNA-loaded hepatocytes (p < 0.05). YHK exerted a significant protection against DPPH radical-scavenging activity in the "old" group (p versus sylibin) and against lipophilic generators in both age groups (p < 0.05 versus sylibin). Both compounds were ineffective on age-related increase of surface-charge density. These preliminary data suggest that age per se enhances the vulnerability of hepatocytes to xenobiotics, whereas some safe nutraceuticals seem to exert significant protective effects.