RESUMEN
Lupus miliaris disseminatus faciei (LMDF) is a chronic inflammatory dermatosis of unknown aetiology, most often seen in young adults. Although many treatments for LMDF exist, treatment guidelines have not been developed, and response to therapy is generally unpredictable. We present the results of transcriptomic analysis of LMDF lesional skin, which revealed a variety of differentially expressed genes linking LMDF to alterations in innate and adaptive T helper 1 immunity. Immunohistochemical analysis was also performed, identifying similar changes in T-cell immune responses. Given evidence for increased tumour necrosis factor (TNF) pathway activity, our patient, who had previously been refractory to multiple treatments, was initiated on TNF inhibitor therapy with excellent response. This characterization of the LMDF immune response may lead to improved treatment of this condition.
Asunto(s)
Dermatosis Facial/inmunología , Granuloma/tratamiento farmacológico , Infliximab/uso terapéutico , Rosácea/tratamiento farmacológico , Inhibidores del Factor de Necrosis Tumoral/uso terapéutico , Administración Intravenosa , Enfermedad Crónica , Quimioterapia Combinada/métodos , Dermatosis Facial/genética , Dermatosis Facial/patología , Perfilación de la Expresión Génica/métodos , Granuloma/diagnóstico , Granuloma/inmunología , Humanos , Inmunidad Celular/inmunología , Inmunohistoquímica/métodos , Inmunosupresores/administración & dosificación , Inmunosupresores/uso terapéutico , Infliximab/administración & dosificación , Masculino , Metotrexato/administración & dosificación , Metotrexato/uso terapéutico , Rosácea/diagnóstico , Rosácea/inmunología , Linfocitos T/inmunología , Células TH1/inmunología , Resultado del Tratamiento , Inhibidores del Factor de Necrosis Tumoral/administración & dosificación , Adulto JovenRESUMEN
Using a previously developed primer system, nifH gene fragments 450 nucleotides long were amplified, cloned, and sequenced for representatives of nitrogen-fixing methanotrophic bacteria of the genera Methylococcus, Methylocystis and Methylosinus. Fragments of nifH genes were also detected and sequenced in representatives of the genera Methylomonas and Methylobacter, which were not considered diazotrophs until recently. Phylogenetic analysis revealed remoteness of nifH genes sequences of methanotroph types I and II. At the same time, close relationship was found between nifH of type I methanotrophs and representatives of gamma-proteobacteria and between nifH genes of type II methanotrophs and representatives of alpha-proteobacteria. The results obtained in this study are in good accordance with the data of phylogenetic analysis based on 16S rRNA sequence comparison with the only exception of Methylococcus capsulatus strains, whose nifH genes proved to be closely related to nifH genes of Methylocystis and Methylosinus representatives. Our findings extend the database of primary sequences of nifH genes and allow the contribution of methanotrophs to the process of nitrogen fixation to be estimated.
Asunto(s)
Euryarchaeota/genética , Genes Bacterianos , Fijación del Nitrógeno/genética , Oxidorreductasas/genética , Secuencia de Bases , Clonación Molecular , Cartilla de ADN , ADN Bacteriano , Euryarchaeota/clasificación , FilogeniaRESUMEN
Based on the analysis of the nifH gene nucleotide sequences from GenBank, a system of primers was developed that makes it possible to obtain 370- and 470-bp PCR fragments of the nifH gene of nitrogen-fixing bacteria and archaea. The effectiveness of the proposed system for revealing the presence of nifH genes was demonstrated by PCR on the DNA isolated from nitrogen-fixing prokaryotes for which the primary structure of these genes is known and which belong to different taxonomic groups. nifH sequences of nitrogen-fixing prokaryotes of the genera Xanthobacter, Beijerinckia, and Methanosarcina, for which the capacity for nitrogen fixation was demonstrated earlier, but no data existed on the nucleotide composition of these genes, were determined and deposited in GenBank.