RESUMEN
In the present work, 19 Mycobacterium bovis isolates from different cats were typified by spoligotyping. We detected nine spoligotypes. There was only one cluster, which grouped 11 of the isolates (57.9%), showing the main spoligotype from cattle from Argentina. The rest of the spoligotypes presented only one isolate each. Five of them were not found in cattle, and were unique and exclusive of cats. The isolates studied show that tuberculosis of bovine origin in cats constitutes a potential public health problem in Buenos Aires region. The identification of genotypes from non-natural hosts could contribute to understand the spread of bovine tuberculosis. This is the first report showing genetic profiles of M. bovis isolates in felines from Argentina.
En el presente trabajo se tipificaron por spoligotyping 19 aislamientos de M. bovis de diferentes gatos. Se detectaron 9 espoligotipos y un único agrupamiento o cluster integrado por 11 aislamientos (57,9%) y relacionado con el principal espoligotipo de bovinos de Argentina. El resto de los espoligotipos detectados presentaron solamente un aislamiento cada uno; 5 de ellos no se encontraron en bovinos y fueron únicos y exclusivos de gatos. La presencia de estos aislamientos indica que la tuberculosis bovina en los gatos constituye un potencial problema de salud pública en la ciudad de Buenos Aires. La identificación de genotipos de aislamientos de M. bovis de hospedadores no convencionales podría contribuir a la mejor comprensión de la diseminación de la tuberculosis bovina. Este es el primer informe en el que se muestran los perfiles genotípicos de aislamientos de M. bovis obtenidos de felinos de Argentina.
Asunto(s)
Animales , Bovinos , Técnicas de Tipificación Bacteriana/métodos , Enfermedades de los Gatos/microbiología , Gatos/microbiología , ADN Bacteriano/análisis , Mycobacterium bovis/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Tuberculosis/veterinaria , Alimentación Animal/efectos adversos , Alimentación Animal/microbiología , Argentina/epidemiología , Enfermedades de los Gatos/epidemiología , Enfermedades de los Gatos/transmisión , ADN Bacteriano/genética , Reservorios de Enfermedades/microbiología , Reservorios de Enfermedades/veterinaria , Contaminación de Alimentos , Microbiología de Alimentos , Pulmón/microbiología , Mycobacterium bovis/clasificación , Mycobacterium bovis/genética , Tuberculosis Bovina/epidemiología , Tuberculosis Bovina/microbiología , Tuberculosis Bovina/transmisión , Tuberculosis/epidemiología , Tuberculosis/microbiología , Tuberculosis/transmisiónRESUMEN
In the present work, 19 Mycobacterium bovis isolates from different cats were typified by spoligotyping. We detected nine spoligotypes. There was only one cluster, which grouped 11 of the isolates (57.9%), showing the main spoligotype from cattle from Argentina. The rest of the spoligotypes presented only one isolate each. Five of them were not found in cattle, and were unique and exclusive of cats. The isolates studied show that tuberculosis of bovine origin in cats constitutes a potential public health problem in Buenos Aires region. The identification of genotypes from non-natural hosts could contribute to understand the spread of bovine tuberculosis. This is the first report showing genetic profiles of M. bovis isolates in felines from Argentina.
Asunto(s)
Técnicas de Tipificación Bacteriana/métodos , Enfermedades de los Gatos/microbiología , Gatos/microbiología , ADN Bacteriano/análisis , Mycobacterium bovis/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Tuberculosis/veterinaria , Alimentación Animal/efectos adversos , Alimentación Animal/microbiología , Animales , Argentina/epidemiología , Enfermedades de los Gatos/epidemiología , Enfermedades de los Gatos/transmisión , Bovinos , ADN Bacteriano/genética , Reservorios de Enfermedades/microbiología , Reservorios de Enfermedades/veterinaria , Contaminación de Alimentos , Microbiología de Alimentos , Pulmón/microbiología , Mycobacterium bovis/clasificación , Mycobacterium bovis/genética , Tuberculosis/epidemiología , Tuberculosis/microbiología , Tuberculosis/transmisión , Tuberculosis Bovina/epidemiología , Tuberculosis Bovina/microbiología , Tuberculosis Bovina/transmisiónRESUMEN
An indirect enzyme-linked immunosorbent assay (IELISA), a competitive ELISA (CELISA), and a fluorescence polarization assay (FPA) for the presumptive serological diagnosis of swine brucellosis were evaluated using two populations of swine sera: sera from brucellosis-free Canadian herds and sera from Argentina selected based on positive reactions in the buffered antigen plate agglutination test (BPAT) and the 2-mercaptoethanol (2-ME) test. In addition, sera from adult swine from which Brucella suis was isolated at least once for each farm of origin were evaluated. The IELISA, CELISA, and FPA specificity values were 99.9, 99.5, and 98. 3%, respectively, and the IELISA, CELISA, and FPA sensitivity values relative to the BPAT and the 2-ME test were 98.9, 96.6, and 93.8%, respectively. Actual sensitivity was assessed by using 37 sera from individual pigs from which B. suis was cultured, and the values obtained were as follows: BPAT, 86.5%; 2-ME test, 81.1%; IELISA, 86.5%; CELISA, 78.5%; and FPA, 80.0%.