RESUMEN

In bovine, single in vitro embryo culture in protein-free medium from Day-6 to Day-7 leads to expanded blastocyst (XB) with improved pregnancy and birth rates after cryopreservation. Under these conditions, early blastocysts (EB) progress to the XB stage at higher rates than morulae (M). However, embryo production with BSA in culture prior to Day-6 leads to low EB rates. We investigated whether a very low FCS concentration (0.1%) in culture from Day-1 to Day-6 would improve EB rates and, subsequently, increase XB rates on Day-7 after single culture in protein-free medium. The quality of embryos produced was evaluated in terms of survival to cryopreservation, apoptosis percentage, lipid accumulation and transfer to recipients. On Day-6, EB rates from embryos cultured with FCS were higher than with BSA (P=0.022). On Day-7, XB rates were higher in embryos from Day-6 EB than from Day-6M, both with and without FCS (P<0.005). After vitrification/warming of Day-7 XB, 100% embryos survived at 24h in all treatments, and total cell number and apoptosis percentage were not affected by the presence of FCS or embryonic stage on Day-6. Cryopreserved and fresh embryos produced with FCS until Day-6, and then deprived of protein and cultured individually, led to pregnancies after ET. In conclusion, minute FCS concentration improves EB rates on Day-6 leading, after one-day single culture without protein, to more XBs. The quality of XB produced with FCS compares well with XB produced with BSA in terms of apoptosis, lipid accumulation and pregnancy.

Asunto(s)

RESUMEN

OBJECTIVES: Occasionally, the Otolaryngologist needs to accede quickly to medical information in order to retrieve data or to solve doubts. In this way, it seems to be of interest to design a clinical guide that could be consulted as a PDA program (Pocket PC and Palm versions), in a personal computer or Internet. MATERIAL AND METHODS: Clinical contents of protocols, guides, and articles, preferably based on the evidence and the clinical experience of 102 authors. Implementation of a program that allows the guide to be used in a PDA, to update it or to add notes using tools that could be downloaded from a web page. RESULTS: A clinical practice guide SEORL-PDA version 1.1, with thirteen ENT's topics, calculation algorithms, internal links and possibility of update the content and to add notes. DISCUSSION: SEORL-PDA is a clinical guide in PDA format, with enough information and possibilities. Its downloading and installation is easily done following the instructions of the web created with these purposes.

Asunto(s)

RESUMEN

Objetivos: En ocasiones el otorrinolaringólogo precisa consultar la información médica de forma rápida para tomar decisiones, aclarar dudas o instaurar tratamientos. Por ello, es de interés diseñar un programa basado en una guía clínica que pueda ser consultada en un ordenador personal, a través de Internet o instalable en una PDA (versiones Pocket PC y Palm). Material y métodos: Contenidos clínicos de protocolos, guías, vías y artículos, preferentemente basados en la evidencia y en la experiencia de 102 autores. Implementación informática que permita usar la guía en una PDA y actualizar o añadir notas a los contenidos a través de herramientas que se descargan desde una página web. Resultados: Guía de práctica clínica SEORL-PDA® versión 1.1 con trece temas de ORL, algoritmos de cálculo, enlaces internos y posibilidad de actualizaciones y notas. Discusión: SEORL-PDA® es una guía clínica en PDA con gran cantidad de datos y posibilidades. Su descarga e instalación se realiza fácilmente siguiendo las instrucciones de la web creada a tal efecto

Objectives: Occasionally, the Otolaryngologist needs to accede quickly to medical information in order to retrieve data or to solve doubts. In this way, it seems to be of interest to design a clinical guide that could be consulted as a PDA program (Pocket PC and Palm versions), in a personal computer or Internet. Material and methods: Clinical contents of protocols, guides, and articles, preferably based on the evidence and the clinical experience of 102 authors. Implementation of a program that allows the guide to be used in a PDA, to update it or to add notes using tools that could be downloaded from a web page. Results: A clinical practice guide SEORLPDA ® version 1.1, with thirteen ENT's topics, calculation algorithms, internal links and possibility of update the content and to add notes. Discussion: SEORL-PDA® is a clinical guide in PDA format, with enough information and possibilities. Its downloading and installation is easily done following the instructions of the web created with these purposes

Asunto(s)

RESUMEN

Due to multimedia technologies, the medical information has taken an extraordinary growing. Nevertheless, the education at University will turn out to be a more complex pedagogic process. We present a multimedia educational material (MEM), developed as an online course to "Seminars of Otolaryngology", and available free at the University website. MEM was designed and edited by, ENT teachers, pedagogues, and image and computer experts. It has been structured as a series of modules that integrate texts, images, movie clips, video, audio and animations. The schematic presentation stands out with progressive access to the topics through internal and external links. The pedagogic resources give additional support by learning objectives, glossary, references, index and searcher. Finally, the activities and instrumental simulations complete the training in diagnosis and treatment. The MEM is an interactive useful software program for medical training in Otolaryngology, without avoiding the key figure of the teacher.

Asunto(s)

RESUMEN

La información médica ha tenido un empuje extraordinario por las tecnologías multimedia. Sin embargo, la formación universitaria debe ser un proceso pedagógico más complejo. Presentamos un material educativo multimedia (MEM), adaptado como "Seminarios de Otorrinolaringología", al que se accede libremente desde una plataforma en red vinculada a la universidad. En la elaboración del MEM participaron profesores ORL, pedagogos, diseñadores gráficos e informáticos. Está estructurado en módulos de contenidos que integran textos, imágenes, vídeos, audios y animaciones. Destaca la presentación esquemática con acceso progresivo a contenidos a través de enlaces y vínculos. Las imágenes reales marcadas y los vídeos completan este aspecto descriptivo. Los recursos pedagógicos: objetivos, plan trabajo, glosario, referencias, índice y buscador apoyan este material. Las actividades y simulaciones instrumentales completan la formación en el diagnóstico y tratamiento. El MEM es una herramienta útil en la enseñanza universitaria de la Otorrinolaringología, sin desplazar la figura clave del profesor

Due to multimedia technologies, the medical information has taken an extraordinary growing. Nevertheless, the education at University will turn out to be a more complex pedagogic process. We present a multimedia educational material (MEM), developed as an online course to "Seminars of Otolaryngology", and available free at the University website. MEM was designed and edited by, ENT teachers, pedagogues, and image and computer experts. It has been structured as a series of modules that integrate texts, images, movie clips, video, audio and animations. The schematic presentation stands out with progressive access to the topics through internal and external links. The pedagogic resources give additional support by learning objectives, glossary, references, index and searcher. Finally, the activities and instrumental simulations complete the training in diagnosis and treatment. The MEM is an interactive useful software program for medical training in Otolaryngology, without avoiding the key figure of the teacher

Asunto(s)

RESUMEN

OBJECTIVE: To investigate the diagnostic sensitivity and prognosis predicting of DNA image cytometry in colorectal carcinoma. STUDY DESIGN: We studied the ploidy status and other DNA cytometric parameters in 68 patients with colorectal carcinoma. In addition, clinical-histologic and follow-up information was collected for at least five years. RESULTS: DNA histograms were available in all cases, showing a diploid DNA distribution pattern in 6 (8.8%), tetraploid in 21 (30.9%), hyperdiploid in 20 (29.4%) and hypertetraploid in 21 (30.9%). The differences in the correlation study between cytometric parameters and pathologic features were not statistically significant. Ploidy status and DNA malignancy grade were individually related to five-year survival (P < .005 and P < .05). CONCLUSION: The data show that DNA image cytometry can provide valuable prognostic information on colorectal carcinomas and may prove useful in guiding adjuvant therapy in these patients.

Asunto(s)

RESUMEN

The limits of the vestibular nuclei and accessory groups were analyzed in 7 rats. This information was taken using camera lucida drawings of the vestibular nuclei and their neurons in serial sections. The study of the morphometric parameters of the neurons (area and maximum diameter) were accomplished by means of a program for image analysis. The cells of the superior nucleus had an area and maximum diameter of 302.05 microns2 and 24.05 microns, those of the medial nucleus 222.98 microns2 and 20.49 microns, those of the lateral nucleus 609.94 microns2 and 34.21 microns, and the descending nucleus cells 327.55 microns2 and 25.24 microns. The cellular parameters of the associated groups were also studied and the distribution of them in several intervals was established.

Asunto(s)