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BACKGROUND: Translating knowledge to improve paediatric rehabilitation has become a research area of interest. This study describes the development and evaluation of an online conference that brought together perspectives of individuals with cerebral palsy (CP), families, health care professionals, and researchers to discuss the daily living of individuals with CP. METHODS: We anchored the development and implementation of the online conference in the action cycle of the Knowledge to Action Framework. To develop the meeting, we included representatives from each stakeholder group in the programme committee. The conference programme was designed having the lifespan perspective of individuals with CP, from birth to adulthood, as its central core, with themes related to daily living (e.g., self-care, mobility, and continuing education). Participants' satisfaction with the conference was assessed using an anonymized online survey sent to all participants. RESULTS: The conference had 1656 attendees, of whom 675 answered the online satisfaction survey. Most participants rated the structure of the conference (i.e., quality of the technical support, audio and video, and online platform) and discussed topics (i.e., relevance, content, discussion, speakers, and available time) positively. CONCLUSION: Collaborative conferences that include stakeholders throughout the planning and implementation are a viable, effective knowledge translation strategy that allows for sharing experiences and disseminating knowledge among families and individuals with CP, health care professionals, and researchers.
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Parálisis Cerebral , Niño , Humanos , Parálisis Cerebral/rehabilitación , Personal de Salud , Autocuidado , Educación ContinuaRESUMEN
Small slaughterhouses generate biowaste, which for economic reasons, is generally destined for composting. Inoculating appropriate microorganisms can improve biodegradation efficiency and mitigate odor generation during the composting process and can give rise to composts with neutral or pleasant odors. Therefore, the aim of this study was to compare the odor intensity reduction of compost generated with and without a formulated inoculum (Saccharomyces cerevisiae, Bacillus subtilis, and Rhodopseudomonas palustris). A set of experimental data was collected and analyzed according to the German "Verein Deutscher Ingenieure" odor protocol. The results showed that adding microorganisms was effective in reducing unpleasant odors in all three composts generated from swine, cattle, and poultry slaughterhouse by-products during both summer and winter seasons. Additionally, soil odor was predominant in composts that were inoculated in the two tested seasons (i.e., summer and winter). On the other hand, composts without inoculation had odors similar to peat for swine compost, ammonia for cattle compost, and manure for poultry compost, regardless of the season tested. Overall, composting process with appropriate inoculum can help in the correct disposal of slaughterhouse wastes by transforming organic matter into composts, which can have economic and environmental value as a soil conditioner and/or fertilizer.
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Compostaje , Animales , Bovinos , Porcinos , Mataderos , Odorantes/prevención & control , Suelo , Biodegradación Ambiental , EstiércolRESUMEN
BACKGROUND: Individuals with unilateral spastic cerebral palsy (USCP) often show difficulties using their hands during activities of daily living. OBJECTIVE: To investigate the factors that interfere with hand use during bimanual activities in children and adolescents with USCP. METHODS: We conducted a cross-sectional study with 102 children and adolescents with USCP, aged 6 to 18 years. We collected information with the caregivers about the classification of the child's manual ability, according to the Manual Ability Classification System (MACS); child's age; side of the involvement; Children's Hand-Use Experience Questionnaire- CHEQ2.0. Cluster analysis identified groups of children and adolescents who performed CHEQ activities with or without assistance. Multiple linear regression analyses identified the contribution of the factors: age, sex, MACS level, side of hemiparesis, and clusters of assistance, on the outcomes of efficacy, time, and feeling bothered. RESULTS: MACS and clusters of assistance explained the variance in efficacy (p<0.05; R2=0.31) and time (p<0.05; R2=0.37). MACS explained 22% of the variance in feeling bothered. Children and adolescents with increased difficulty to perform activities that involve hand use (i.e., MACS III) and who receive assistance during most bimanual activities showed less efficacy of use, were slower in their performance, and presented greater feeling of being bothered. CONCLUSION: Assistance in bimanual activities and MACS level contributed to explain the efficacy of use, time, and feeling bothered in performing bimanual activities. Intervention strategies aimed at promoting the performance of bimanual activities in the daily routine of children with USCP should consider these outcomes.
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Parálisis Cerebral , Niño , Humanos , Adolescente , Actividades Cotidianas , Estudios Transversales , Mano , Extremidad SuperiorRESUMEN
OBJECTIVE: Explore in depth the experiences of women giving birth during the COVID-19 pandemic in Indonesia. METHODS: Qualitative research with a descriptive phenomenological. A total of 16 participants did not suffer from COVID-19 and gave birth during the COVID-19 pandemic in the community setting area. RESULTS: There are five themes: 1: Feelings of anxiety and fear of giving birth in hospitals are experienced by women giving birth during the COVID-19 pandemic, 2. Screening for COVID and health protocols applied in hospitals during the COVID-19 pandemic, 3. Support from husbands, families, and health workers is needed when giving birth during the COVID-19 pandemic, 4. Lack of family visits hours is an obstacle during childbirth, particularly during the COVID-19 pandemic, and 5: Bonding attachment between mother and baby while at the hospital. CONCLUSION: Optimal maternity nursing care and supporting health facilities and public policies will help mothers give birth during the COVID-19 pandemic in Indonesia.
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COVID-19 , Pandemias , Embarazo , Femenino , Humanos , Indonesia/epidemiología , COVID-19/epidemiología , Parto Obstétrico/métodos , Madres , Investigación CualitativaRESUMEN
ABSTRACT Objective: Explore in depth the experiences of women giving birth during the COVID-19 pandemic in Indonesia. Methods: Qualitative research with a descriptive phenomenological. A total of 16 participants did not suffer from COVID-19 and gave birth during the COVID-19 pandemic in the community setting area. Results: There are five themes: 1: Feelings of anxiety and fear of giving birth in hospitals are experienced by women giving birth during the COVID-19 pandemic, 2. Screening for COVID and health protocols applied in hospitals during the COVID-19 pandemic, 3. Support from husbands, families, and health workers is needed when giving birth during the COVID-19 pandemic, 4. Lack of family visits hours is an obstacle during childbirth, particularly during the COVID-19 pandemic, and 5: Bonding attachment between mother and baby while at the hospital. Conclusion: Optimal maternity nursing care and supporting health facilities and public policies will help mothers give birth during the COVID-19 pandemic in Indonesia.
RESUMO Objetivo: Explorar em profundidade as experiências de mulheres que deram à luz durante a pandemia de COVID-19 na Indonésia. Métodos: Pesquisa qualitativa com abordagem fenomenológica descritiva. Um total de 16 participantes que não sofriam de COVID-19 deram à luz durante a pandemia de COVID-19 no ambiente comunitário. Resultados: Há cinco temas: 1: Sentimentos de ansiedade e medo de dar à luz em hospitais são vivenciados por mulheres que dão à luz durante a pandemia de COVID-19, 2. Triagem para COVID e protocolos de saúde aplicados em hospitais durante a pandemia de COVID-19, 3. O apoio de maridos, famílias e profissionais de saúde é necessário durante o parto na pandemia de COVID-19, 4. A falta de horário para visitas familiares é um obstáculo durante o parto, principalmente durante a pandemia de COVID-19, e 5: Vínculo entre mãe e bebê no hospital. Conclusão: Os cuidados ideais de enfermagem na maternidade e o apoio das unidades de saúde e políticas públicas ajudarão as mães a dar à luz durante a pandemia de COVID-19 na Indonésia.
RESUMEN Objetivo: Explorar en profundidad las experiencias de las mujeres que dieron a luz durante la pandemia de COVID-19 en Indonesia. Métodos: Investigación cualitativa con enfoque fenomenológico descriptivo. Un total de 16 participantes que no sufrieron de COVID-19 dieron a luz durante la pandemia de COVID-19 en el entorno comunitario. Resultados: Hay cinco temas: 1: Los sentimientos de ansiedad y miedo de dar a luz en los hospitales son experimentados por las mujeres que dan a luz durante la pandemia de COVID-19, 2. Detección de COVID y protocolos de salud aplicados en los hospitales durante la pandemia de COVID-19, 3. Se necesita el apoyo de esposos, familias y trabajadores de la salud cuando se da a luz durante la pandemia de COVID-19, 4. La falta de horarios de visitas familiares es un obstáculo durante el parto, particularmente durante la pandemia de COVID-19, y 5: Vínculo entre madre y bebé mientras están en el hospital. Conclusión: la atención de enfermería de maternidad óptima y el apoyo de los centros de salud y las políticas públicas ayudarán a las madres a dar a luz durante la pandemia de COVID-19 en Indonesia.
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In this article, the environmental sustainability of a circular economy concept applied to the management of biowaste was studied. To achieve this goal, the composting performance, compost-amended soil health, and phytotoxicity were assessed in the case of management of solid waste from a small swine slaughterhouse. Microorganisms present in a similar composting process were used as inoculums to improve the efficiency of composting. Addition of the inoculum promoted a faster and more efficient composting process than composting without the inoculum. The physical, chemical, and microbiological characteristics of soil were considered to be improved after compost application. Phytotoxicity tests in soils with and without compost amendment showed that a soil-compost mixture (90:10 and 70:30 mass ratios) was not phytotoxic to the plant species Sorghum saccharatum and Lepidium sativum, and that soil with compost showed higher plant biomass growth than that without compost amendment. The triple bottom line methodology used in this study can help in the assessment of circular economy activity in relation to the environmentally sustainable management of solid waste generated in small swine slaughterhouses.
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The composting process is an option for acceptable environmental management of cattle slaughterhouse by-products. The goals of this article were (i) to make a low-cost inoculum using popular supermarket ingredients and microorganisms that are already present in the composting environment, and (ii) to compare the efficiency of the composting process with and without the application of formulated inoculum. Initially, a consortium of microorganisms already present in the composting environment (Saccharomyces cerevisiae, Bacillus subtilis, and Rhodopseudomonas palustris) was prepared in a low-cost culture medium for use as an inoculum for the composting process. The composting process with the addition of the inoculum was more efficient than the composting process without the inoculum, in terms of both the chemical composition and the process efficiency, but mainly in relation to the time required for composting, with the mean times for decay of 50% of the windrows' temperature (taking in to account the difference between internal and external windrow temperatures) being 96 days without inoculum and 65 days with inoculum. Thus, inoculum made with low-cost supermarket products reduced the composting time and yielded compost of better quality.
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Compostaje , Mataderos , Animales , Bovinos , Suelo/química , TemperaturaRESUMEN
ABSTRACT: Cognitive functional therapy (CFT) is a physiotherapy-led intervention that has evolved from an integration of foundational behavioral psychology and neuroscience within the physiotherapist practice directed at the multidimensional nature of chronic low back pain (CLBP). The current evidence about the comparative effectiveness of CFT for CLBP is still scarce. We aimed to investigate whether CFT is more effective than core training exercise and manual therapy (CORE-MT) in pain and disability in patients with CLBP. A total of 148 adults with CLBP were randomly assigned to receive 5 one-hour individualized sessions of either CFT (n = 74) or CORE-MT (n = 74) within a period of 8 weeks. Primary outcomes were pain intensity (numeric pain rating scale, 0-10) and disability (Oswestry Disability Index, 0-100) at 8 weeks. Patients were assessed preintervention, at 8 weeks and 6 and 12 months after the first treatment session. Altogether, 97.3% (n = 72) of patients in each intervention group completed the 8 weeks of the trial. Cognitive functional therapy was more effective than CORE-MT in disability at 8 weeks (MD = -4.75; 95% CI -8.38 to -1.11; P = 0.011, effect size= 0.55) but not in pain intensity (MD = -0.04; 95% CI -0.79 to 0.71; P = 0.916). Treatment with CFT reduced disability, but the difference was not clinically important compared with CORE-MT postintervention (short term) in patients with CLBP. There was no difference in pain intensity between interventions, and the treatment effect was not maintained in the mid-term and long-term follow-ups.
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Dolor Crónico , Terapia Cognitivo-Conductual , Dolor de la Región Lumbar , Manipulaciones Musculoesqueléticas , Adulto , Humanos , Dolor de la Región Lumbar/terapia , Dolor de la Región Lumbar/psicología , Terapia Cognitivo-Conductual/métodos , Modalidades de Fisioterapia , Cognición , Terapia por Ejercicio/métodos , Dolor Crónico/terapia , Dolor Crónico/psicologíaRESUMEN
A chondral injury is a limiting disease that can affect the quality of life and be an economic burden due to the cost of immediate treatment and loss in work productivity. If left untreated, such an injury may progress to osteoarthritis, a degenerative and debilitating joint disease characterized by pain and functional impairment. Mesenchymal stromal cells (MSCs), which have immune-modulatory properties and the ability to differentiate into chondroblasts and osteoblasts, are a predictable source for the treatment of cartilage injuries. This article presents tools to evaluate cartilage restoration by tissue engineering and cell therapy treatment in a translational and preclinical large animal model. In this controlled experimental study with 14 miniature pigs, a scaffold-free tissue engineering construct (TEC) derived from dental pulp and synovial MSCs for cartilage therapy was tested. Total thickness cartilage defects were performed in both posterior knees. The defect was left empty in one of the knees, and the other received the TEC. The tissue repair was morphologically assessed by magnetic resonance imaging (MRI) using the three-dimensional double echo steady-state (3D-DESS) sequence, and compositional assessment was carried out based on the T2 mapping technique. The osteochondral specimens were fixed for histopathology, decalcified, subjected to standard histological processing, sectioned, and stained with hematoxylin and eosin. The sections stained for immunohistochemical detection of collagen types were digested with pepsin and chondroitinase and incubated with antibodies against them. The mechanical evaluation involved analysis of Young's modulus of the cartilage samples based on the indentation and maximum compression test. In addition, a finite element model was used to simulate and characterize properties of the osteochondral block. At 6 months after surgery, there were no complications with the animals and the MRI, histological, immunohistochemical, and biomechanical evaluations proved to be effective and qualified to differentiate good quality chondral repair from inadequate repair tissue. The proposed methods were feasible and capable to properly evaluate the defect filled with TEC containing stromal cells after 6 months of follow-up in a large animal model for articular cartilage restoration. Impact Statement Articular chondral injuries are prevalent and represent an economic burden due to the cost of treatment. The engineering of cartilage tissue can promote the repair of chondral injuries and is dependent on selecting appropriate cells and biocompatible frameworks. In this article, methods for evaluation of a scaffold-free cell delivery system made from mesenchymal stromal cells were present in a translational study that allows further clinical safety and efficacy trials.
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Cartílago Articular , Ingeniería de Tejidos , Animales , Cartílago Articular/diagnóstico por imagen , Cartílago Articular/patología , Tratamiento Basado en Trasplante de Células y Tejidos , Calidad de Vida , Porcinos , Ingeniería de Tejidos/métodos , Andamios del TejidoRESUMEN
OBJECTIVE: A burn injury has two defined areas: central necrosis and an adjacent area of ischaemia, which may or may not progress to necrosis. The concentration of nitric oxide (NO) increases after burn injury and may originate from potent oxidising agents. Methylene blue (MB) may act as an antioxidant and is supposed to reduce burn progression. This investigation was carried out to evaluate the effects of intradermal MB on necrosis progression in burns. METHODS: Full-thickness burn injuries were performed by applying a heated metal comb on the shaved back of male Wistar rats. The animals were divided into three groups: Control (C, n=7); MB (2mg/kg) one hour after burn injury (MB1h, n=11); and MB (2mg/kg) six hours after burn injury (MB6h, n=8). After seven days the lesions were photographed for visual assessment of burn necrosis; full-thickness cuts of lesions were dyed with Masson and Giemsa for microscopic histopathology; and tissue fragments of unburned interspaces were processed for chemiluminescence with nitrite/nitrate (NOX) and malondialdehyde (MDA) as oxidative stress markers. RESULTS: No statistically significant differences between groups were observed during visual analysis and NOX dosage. However, in microscopic analysis, the MB1h and MB6h groups showed smaller areas of necrosis, less inflammatory infiltration, and a more significant extension of interspaces. Furthermore, the dosage of MDA revealed that the MB1h group showed lower values when compared with the control group (p=0.001). CONCLUSIONS: The study provided good evidence that MB intradermal injection can reduce necrosis progression in ischaemic perilesional areas and suggests an alternative to treating burns.
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Quemaduras , Azul de Metileno , Animales , Quemaduras/tratamiento farmacológico , Modelos Animales de Enfermedad , Masculino , Azul de Metileno/farmacología , Azul de Metileno/uso terapéutico , Necrosis , Ratas , Ratas WistarRESUMEN
This work aimed to evaluate the impact of veterinary antibiotics on biomass phytoproductivity and soil enzyme activity. The soil was sampled in the city of Camboriú (state of SC, Brazil). The soil enzyme activity was assessed through hydrolysis of fluorescein diacetate (FDA), while phytotoxicity was tested using Lactuca sativa (lettuce). Results showed that the most appropriate exposure time to assess the impact of antibiotics on soil microbiology was 24 h, while the incubation time of 3 h was the most appropriate for FDA hydrolysis. Ampicillin and Amoxicillin at the tested concentrations did not interfere with the enzyme activity of the soil microbiota, while Oxytetracycline and Neomycin showed a significant reduction in soil enzyme activity. For the dry and wet biomass of lettuce, 2% Colistin and 1% Ampicillin were the treatments that reduced lettuce biomass. Hence, the use of excessive antibiotics in animal production may lead to environmental impacts and, in the future, to public health problems.
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Contaminantes del Suelo , Suelo , Animales , Antibacterianos/toxicidad , Brasil , Granjas , Lactuca , Microbiología del Suelo , Contaminantes del Suelo/toxicidadRESUMEN
Knowledge translation (KT) is gaining attention in the pediatric rehabilitation field. Nossa Casa Institute is the first organization in Brazil aiming to foster cerebral palsy (CP) awareness and empower families by discussing reliable information. This study aims to build a network where individuals with CP and their families, researchers, health care professionals, and services can communicate and share experiences. In this article, we describe the experience of planning and conducting an educational and interactive online workshop to foster principles of family-centered service (FCS). We used the action cycle from the Knowledge to Action (KTA) framework to describe and ground the proposed activities. In Module 1, "Challenges and barriers to incorporate family-centered principles," we discussed the historical perspective, main principles, and challenges related to FCS implementation. Module 2, "What is my contribution to the family-centered service?" was aimed to foster strategies to improve the implementation of principles of FCS in the care of children with disabilities. In Module 3, "What can we do together?" the groups presented their ideas and suggestions. This interactive and educational workshop was an opportunity for Nossa Casa Institute to disseminate accessible and reliable information regarding FCS and to empower families to participate actively in the rehabilitation process and advocate for the best provision of care for their children. Future actions of Nossa Casa Institute include the coordination of a national conference to connect families, individuals with CP, healthcare and rehabilitation professionals, and researchers. There is also a need, and opportunity, for formal evaluation of these KT activities.
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Resumen El artículo aborda la perspectiva de profesionales de oncología y cuidados paliativos de Argentina sobre la planificación anticipada de los cuidados en el final de la vida de pacientes con enfermedades oncológicas avanzadas. A partir de un diseño de investigación cualitativa, basado en entrevistas semi-estructuradas con profesionales de diferentes disciplinas, se analizaron las dificultades para la participación de los y las pacientes en el proceso de toma de decisiones y en los procesos de consentimiento informado en torno a los cuidados en el final de la vida. Pese a valorar la planificación anticipada del cuidado e instrumentos como las directivas anticipadas, se identifican barreras para la implementación de estas prácticas. La falta de herramientas comunicacionales para abordar este tipo de conversaciones con los pacientes, el ocultamiento de la información sobre el diagnóstico y/o el pronóstico de la enfermedad, la falta de articulación entre equipos que realizan el seguimiento de pacientes con enfermedad avanzada, y la resistencia de familiares y pacientes, limitan los márgenes de decisión y consentimiento por parte de los pacientes.
Abstract The article addresses the perspective of oncologist and palliative care professionals of Argentina on the advance end of life care planning for patients with advanced cancer. Based on semi-structured interviews with professionals from different disciplines, the paper explores barriers for the patient participation in the decision-making process in end of life care. Despite the acceptance of advance care planning and instruments such as advance directives, barriers to the implementation of these practices are identified. The lack of communication skills to deal with this type of conversation with patients, the concealment of information on the diagnosis and prognosis of the disease, the lack of coordination between teams that keep track of patients with advanced disease, and the resistance from relatives and patients, limit the margins of decision and consent by patients.
Resumo O artigo aborda a perspectiva dos profissionais de oncologia e cuidados paliativos na Argentina sobre o planejamento antecipado dos cuidados de fim de vida para pacientes com doenças oncológicas avançadas. Partindo de um desenho de pesquisa qualitativa, com base em entrevistas semiestruturadas com profissionais de diferentes disciplinas, o artigo analisa as dificuldades para a participação dos pacientes no processo de tomada de decisão e nos processos de consentimento informado em torno aos cuidados de fim de vida. Apesar de valorizar o planejamento do cuidado antecipado e instrumentos como as diretrizes antecipadas, identificam-se barreiras para a implantação dessas práticas. A falta de instrumentos de comunicação para atender esse tipo de conversa com os pacientes, o encobrimento do diagnóstico e / ou prognóstico da doença, a falta de articulação entre as equipes que acompanham os pacientes com doença avançada e a resistência dos familiares e pacientes, limitam as margens de decisão e consentimento por parte dos pacientes.
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Humanos , Cuidado Terminal , Directivas Anticipadas , Enfermo Terminal , Toma de Decisiones , Consentimiento Informado , Neoplasias , Grupo de Atención al Paciente , Pacientes , Argentina , Cuidados Paliativos al Final de la Vida , Enfermedad Crítica , Investigación CualitativaRESUMEN
In this work, we propose a novel application of ERIC-PCR technique to study DNA damage after ultraviolet radiation (UV) and peracetic acid (PAA) treatment for water disinfection purpose. The efficacy of both treatments on E. coli suspension was evaluated by two approaches: through monitoring of inactivation by conventional culture technique, and by analyzing DNA damage with ERIC-PCR. All the experiments were carried out in a batch reactor, using three intensities of UV-C radiation (10.5, 4.2 and 2.1 mW/cm2) and different PAA concentrations (4 to 16 ppm). Both treatments produced bacterial inactivation in a dose-response fashion. Based on the results of bacterial count we obtained an index of inactivation (INACI). For each sample, DNA extraction was performed and evaluated by ERIC-PCR. Qualitative modifications were observed in ERIC-PCR band patterns for all the UV-C radiation intensities used, but no changes were detected at any of the PAA concentrations. The banding pattern modifications observed are consequence of the interruption of Taq polymerase enzyme amplification-activity, caused by the presence of alterations on the DNA structure (dimer and hydrates formation). Furthermore, an index was proposed to measure DNA damage (DNADI) regarding the changes in the relative optical density values of the amplification products. A linear correlation was obtained with a high correspondence between the inactivation index (INACI) and the DNA damage index (DNADI), that was expressed as DNADI = 0.05881×INACI. This approach proves that ERIC-PCR is a feasible and valuable tool for detecting and quantifying DNA damage and it may provide a useful strategy for bacterial identification, tracking changes in DNA and providing reliable and reproducible data.
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Daño del ADN , Enterobacteriaceae/genética , Purificación del Agua/métodos , Daño del ADN/efectos de los fármacos , Daño del ADN/efectos de la radiación , ADN Bacteriano/química , ADN Bacteriano/metabolismo , Desinfección/métodos , Ácido Peracético/farmacología , Reacción en Cadena de la Polimerasa , Rayos UltravioletaRESUMEN
O presente trabalho visa investigar as dinâmicas psicológicas das relações entre mães e filhas, utilizando como base a perspectiva psicanalítica. Mais especificamente, buscou-se compreender as problemáticas existentes quando há um grau elevado de indiferenciação entre a menina e sua mãe. Para isso, realizou-se uma articulação entre um estudo de caso e conceitos da psicanálise que fundamentam as cenas clínicas apresentadas. O estudo de caso concretizou-se a partir de um atendimento psicológico de uma criança que apresentava importantes sofrimentos oriundos de uma dificuldade de separação da figura materna. Assim, pretende-se fornecer uma melhor compreensão a respeito das vicissitudes de uma relação simbiótica com a mãe e as consequências de tal para a constituição da vida psíquica da mulher.
Este trabajo pretende investigar cómo la dinámica psicológica de las relaciones entre madres e hijas, como base de la perspectiva psicoanalítica. Más específicamente, pretendemos comprender los problemas que pueden surgir en una relación altamente indiferenciada entre madre e hija. Por lo tanto, se pretende proporcionar una mejor comprensión de la relación simbiótica con una madre y sus consecuencias para la constitución psíquica de las niñas. A partir de este estudio de caso, se lleva a cabo una discusión en línea con nuestros conceptos teóricos constructivos que diferentes autores del psicoanálisis han realizado sobre el tema de la transmisión de la feminidad entre madres e hijas.
This work aims to investigate how the psychological dynamics of relations between mothers and daughters, as a basis the psychoanalytic perspective. More specifically, we intend to comprehend the problems that may emerge in a highly undifferentiated relationship between mother and daughter. Thus, it is intended to provide a better understanding of the symbiotic relationship with a mother and its consequences for the the girls psychic constitution. Departing from this case study, a discussion is carried out in line with our constructive theoretical concepts that different authors of psychoanalysis have made on the subject of the transmission of femininity between mothers and daughters.
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Humanos , Femenino , Preescolar , Niño , Psicoterapia , Mujeres , Relaciones Madre-Hijo/psicología , Apego a Objetos , Feminidad , Hostilidad , NarcisismoRESUMEN
PURPOSE: Elbow flexion deficit is a frequent problem in traumatic brachial plexus injuries and reestablishment of this function is the primary treatment goal. When management is delayed, or the initial acute approach fails, free functional transfer of the gracilis muscle for elbow flexion is the treatment of choice. In this report, the authors present the results of a comparison study on different donor nerves (spinal accessory and ulnar) in elbow flexion reconstruction with gracilis flap for traumatic adult brachial plexus injuries. METHODS: Retrospective analysis of patients with both total or partial traumatic brachial plexus injuries was carried out. Of the 38 patients enrolled, 37 were male (97.4%) with a mean age of 28.3 years. The mean follow-up period was 25 months. Postoperative function of the gracilis muscle flap was recorded and patients were divided into two groups according to donor nerve: spinal accessory nerve (SAN) (18 cases), and motor fascicles of the ulnar (ULNAR) (20 cases). RESULTS: Twenty-six cases obtained elbow flexion strength M3 or M4 (68.4%): 0 M0 (0.0%), 4 M1 (10.5%), 8 M2 (21.1%), 9 M3 (23.7%) and 17 M4 (44.7%). The mean interval to first recorded M3 muscular strength was 12.4 months. Functional elbow flexion strength (≥ M3) had the following distribution: SAN 83.3% (15/18) and ULNAR 55.0% (11/20) (p = .086). CONCLUSION: No statistical difference for final muscle strength was found between donor nerve groups.
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Neuropatías del Plexo Braquial/cirugía , Plexo Braquial/lesiones , Lesiones de Codo , Músculo Grácil/trasplante , Rango del Movimiento Articular/fisiología , Adolescente , Adulto , Plexo Braquial/cirugía , Neuropatías del Plexo Braquial/etiología , Estudios de Cohortes , Articulación del Codo/cirugía , Femenino , Estudios de Seguimiento , Colgajos Tisulares Libres/inervación , Colgajos Tisulares Libres/trasplante , Músculo Grácil/inervación , Humanos , Masculino , Persona de Mediana Edad , Transferencia de Nervios/métodos , Traumatismos de los Nervios Periféricos/diagnóstico , Traumatismos de los Nervios Periféricos/cirugía , Modelos de Riesgos Proporcionales , Recuperación de la Función/fisiología , Estudios Retrospectivos , Medición de Riesgo , Estadísticas no Paramétricas , Donantes de Tejidos , Resultado del Tratamiento , Adulto JovenRESUMEN
BACKGROUND: Trypsin from fish species is considered as a cold-adapted enzyme that may find potential biotechnological applications. In this work, the recombinant expression, refolding and activation of Trypsin I (TryI) from Monterey sardine (Sardinops sagax caerulea) are reported. METHODS: TryI was overexpressed in Escherichia coli BL21 as a fusion protein of trypsinogen with thioredoxin. Refolding of trypsinogen I was achieved by dialysis of bacterial inclusion bodies with a recovery of 16.32 mg per liter of Luria broth medium. RESULTS: Before activation, the trypsinogen fusion protein did not show trypsin activity. Trypsinogen I was activated by adding 0.002 U of native TryI purified from the sardine pyloric caeca (nonrecombinant). The activated recombinant trypsin showed three times more activity than the nonrecombinant trypsin alone. CONCLUSION: The described protocol allowed obtaining sufficient amounts of recombinant TryI from Monterey sardine fish for further biochemical and biophysical characterization of its coldadaptation parameters.
Asunto(s)
Escherichia coli , Proteínas de Peces , Peces/genética , Cuerpos de Inclusión , Replegamiento Proteico , Tripsina , Animales , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas de Peces/biosíntesis , Proteínas de Peces/química , Proteínas de Peces/genética , Proteínas de Peces/aislamiento & purificación , Cuerpos de Inclusión/química , Cuerpos de Inclusión/genética , Cuerpos de Inclusión/metabolismo , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Tripsina/biosíntesis , Tripsina/química , Tripsina/genética , Tripsina/aislamiento & purificaciónRESUMEN
This study aimed to evaluate the intestinal morphology, performance and financial analysis of the inclusion of additives in the feed during the productive period of 360 Japanese quails distributed in a completely randomized design in a split plot scheme in time with five treatments and eight repetitions of nine birds each. The treatments were: control, antibiotic, prebiotic, probiotic and synbiotic. The variables were: height, width and width/height ratio of the villi, crypt depth and villus/crypt ratio, feed intake, egg production, egg weight average, egg mass, feed conversion per mass and per dozen eggs and viability, internal rate of return, net present value and cost benefit. The additives in the feed increased height and width of the villi, decreased crypt depth and increased villus/ crypt ratio compared to the control. Feed intake was lower after the inclusion of antibiotics and synbiotics in the feed. Egg production and feed conversion improved after the inclusion of additives in the feed compared with the control. The reduction of feed intake was more pronounced with the addition of antibiotic and synbiotic in the final stage of the productive period of the Japanese quails. The inclusion of antibiotics and synbiotic proved to be more financially viable.