RESUMEN
Energy and structural parameters of monochromanes and trimethylphenols derivatives have been chracterized through the employment of semiempirical methods of quantum chemistry. The correlation has been proved between antiradical, hepatoprotective properties of these compounds and energy and structural of their molecules that confirms the presence of relation between structure activity and electron-donor properties of the investigated monochromanes and trimethylphenols.
Asunto(s)
Antioxidantes/farmacología , Cromanos/farmacología , Hígado/efectos de los fármacos , Modelos Químicos , Fenoles/farmacología , Teoría Cuántica , Animales , Antioxidantes/química , Fenómenos Químicos , Cromanos/química , Transporte de Electrón , Retículo Endoplásmico/efectos de los fármacos , Retículo Endoplásmico/metabolismo , Membranas Intracelulares/efectos de los fármacos , Membranas Intracelulares/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Hígado/metabolismo , Fenoles/química , Ratas , Relación Estructura-ActividadRESUMEN
Using biochemical and physicochemical methods of investigation in vivo, the effect of the substance NC-224, N-, S-chinasolone-derivative, on the lipoperoxidation activity in rat liver endoplasmatic reticulum membranes and nuclear chromatin fractions under tetrachloromethane intoxication have been studied. It was shown that NC-224 has pronounced antioxidant activity which is the biochemical basis of the substance membrane- and genome-protective effects and its ability to restore physicochemical properties of the surface and hydrophobic zones of hepatocyte membranes and structural parameter nuclear chromatin fractions in the conditions of chemical liver injury.
Asunto(s)
Cromatina/efectos de los fármacos , Retículo Endoplásmico/efectos de los fármacos , Lípidos/análisis , Malondialdehído/análisis , Oxiquinolina , Animales , Antioxidantes/farmacología , Hidroxitolueno Butilado/farmacología , Tetracloruro de Carbono/toxicidad , Enfermedad Hepática Inducida por Sustancias y Drogas/tratamiento farmacológico , Enfermedad Hepática Inducida por Sustancias y Drogas/patología , Cromatina/metabolismo , Retículo Endoplásmico/metabolismo , Hepatocitos/efectos de los fármacos , Hepatocitos/patología , Peroxidación de Lípido/efectos de los fármacos , Lípidos/química , Hígado/citología , Hígado/efectos de los fármacos , Hígado/patología , Oxiquinolina/análogos & derivados , Oxiquinolina/farmacología , Ratas , Ratas WistarAsunto(s)
Enfermedad Coronaria/complicaciones , Ecocardiografía Doppler , Contracción Miocárdica/fisiología , Disfunción Ventricular Izquierda/diagnóstico por imagen , Diagnóstico Diferencial , Diástole , Humanos , Reproducibilidad de los Resultados , Disfunción Ventricular Izquierda/complicaciones , Disfunción Ventricular Izquierda/fisiopatologíaRESUMEN
The biochemical mechanisms of genomoprotective action of the new derivatives of pyridinecarbonic acids--PV-3, PV-4 and PV-6 in the conditions of tetrachloromethane chemical cell damage have been studied. This effect is related to their antioxidant influence on LPO processes in transcriptionally active and repressed chromatin fractions of rat liver cells and partial restoration of these fractions structure which has been destroyed during intoxication. PV-4 possesses the most genoprotective activity among the substances studied.
Asunto(s)
Antimutagênicos/farmacología , Tetracloruro de Carbono/farmacología , Ácido Carbónico/farmacología , Genoma , Hígado/efectos de los fármacos , Animales , Ácido Carbónico/química , Masculino , Piridinas/química , Ratas , Ratas WistarRESUMEN
Embikhin causes activation of LPO processes in endoplasmic reticulum and in nuclear chromatine fractions of rat liver cells. The latter is accompanied by the impairment of repressive and active nuclear chromatine fractions structure. Derivate of vitamin E in these conditions renders correcting action on parameters of lipid peroxidation in the investigated subcellular structures, testifying its positive influence on the cell heredity apparatus state. The normalizing action of tocopherol derivative on cytochromes P450 and b5 levels is shown.
Asunto(s)
Cromatina/química , Peroxidación de Lípido/efectos de los fármacos , Hígado/efectos de los fármacos , Mecloretamina/farmacología , Vitamina E/análogos & derivados , Animales , Membrana Celular/efectos de los fármacos , Hígado/metabolismo , Masculino , Conformación Proteica , Ratas , Vitamina E/farmacologíaRESUMEN
The actions of the amizon on the antioxidant activity and free radicaloformation level in the nuclear chromatin fractions of rat liver methan tetrachlorid intoxication was studied using the Fe(+2)-induction biochemiluminescence and microcalorimetry methods. The amizon genomoprotective effect on the chromatin transcriptionally active fraction was shown.