RESUMEN
Bacteria usually produce, release and detect quorum sensing (QS)-based signal molecules, and successively orchestrate gene expression in respond to environmental changes. Pseudoalteromonas are typical marine bacteria, but knowledge on their QS systems is extremely fragmentary. In this study, genome sequencing of Pseudoalteromonas sp. R3 was performed. Accordingly, a QS working model including three sets of hierarchically organized QS systems was proposed in strain R3. Among them, the typical LuxI/R-type QS system using acyl-homoserine lactones (AHLs) as signal molecules was characterized. Sequence similarity analysis indicated luxI encoding AHLs synthase is novel. The luxR encoding AHLs receptor is directly adjacent to luxI downstream. Notably, mutagenesis demonstrated LuxI and LuxR affect each other at transcriptional level, and both control the AHLs formation. Interestingly, it was found that LuxI/R-type QS system positively involves resistance to streptomycin. Thin-layer chromatography analysis showed strain R3 can produce 3-OH-C6-HSL and C8-HSL, which was supported by heterologous expression of LuxI in Escherichia coli. Sequence alignment analysis indicated that the N-terminal region of LuxI is more conservative than the C-terminal region, revealing the importance of N-terminal region in AHLs synthesis. The obtained findings enrich our knowledge on LuxI/R-type QS system in Pseudoalteromonas and its regulation on adaptation to marine environments.
Asunto(s)
Acil-Butirolactonas/metabolismo , Proteínas Bacterianas/genética , Farmacorresistencia Bacteriana/genética , Pseudoalteromonas/genética , Percepción de Quorum/genética , Proteínas Represoras/genética , Transactivadores/genética , Factores de Transcripción/genética , Secuencia de Aminoácidos , Antibacterianos/farmacología , Secuencia de Bases , Regulación Bacteriana de la Expresión Génica/genética , Genoma Bacteriano/genética , Pseudoalteromonas/efectos de los fármacos , Pseudoalteromonas/fisiología , Alineación de Secuencia , Transducción de Señal/genética , Estreptomicina/farmacología , Secuenciación Completa del GenomaRESUMEN
In a practical light emitted diodes (LEDs)-based visible light communication (VLC) system, high-speed transmission is generally limited by the LED bandwidth. To address the bandwidth limitation, a hybrid digital linear and decision-feedback equalization (DFE) is investigated to improve the transmission performance (or spectral efficiency) in the carrier-less amplitude phase modulation (CAP)-based VLC systems. A real-time CAP-VLC transceiver with the hybrid digital equalization is designed, based on which 200 Mb/s transmission is successfully demonstrated over a 15 m VLC link with the commercial red LEDs (bandwidth: 6.5 MHz). In the real-time CAP-VLC system, the baseline wander (BLW) is observed, due to the removal of the low-frequency components with a direct current (DC) block. The BLW effect can be mitigated by increasing the roll-off factor. However, this roll-off factor affects the equalization performance, due to an increased loss in the signal spectrum beyond the system bandwidth. Optimization of the roll-off factor and filter length is required. Experimental results show that, with the optimized real-time transceiver design, the hybrid Wiener/recursive least squares (RLS) and DFE significantly improves the error vector magnitude (EVM) performance compared with the DFE. In addition, the digital signal processing (DSP) complexity is discussed.