RESUMEN
PURPOSE: Angiogenesis appears to be a prominent feature of many hematological disorders, particularly in multiple myeloma (MM). Progression in MM also involves secretion of the metaloproteinases (MMPs). In this study, the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and its receptor, in bone marrow trephine biopsy (TB) of thirty six MM patients before and after the treatment or during progression was examined. The MMP-2 secretion was assessed from the same patients. MATERIAL/METHODS: Immunohistochemical staining of bone marrow specimens for angiogenic factors and microvessel density (MVD) and bone marrow aspirates for Western blot analysis of MMP-2 expression was performed. RESULTS: In active, untreated MM patients, we found statistically significant differences in the expression of angiogenic factors according to the patients after the anti-angiogenic treatment. We found statistical differences of the expression of angiogenic factors between the group of patients with a response after the treatment and the patients who had progression during the treatment. The data showed statistically significant decreased MVD after the treatment. The results showed statistically significant differences between initial secretion of MMP-2 in active, untreated MM patients and patients with a response after the treatment and patients with progression during the treatment. CONCLUSIONS: We showed that not only decreased expression of angiogenic cytokines is present after the anti-angiogenic treatment but also activity of MMP-2 in MM patients who responded to the treatment. Combination therapy with the inhibition of the activity of MMPs could represent an interesting therapeutical approach in MM.
Asunto(s)
Médula Ósea/metabolismo , Médula Ósea/patología , Metaloproteinasa 2 de la Matriz/metabolismo , Mieloma Múltiple/tratamiento farmacológico , Mieloma Múltiple/metabolismo , Neovascularización Patológica , Adulto , Anciano , Inhibidores de la Angiogénesis/uso terapéutico , Biopsia , Células de la Médula Ósea/citología , Citocinas/metabolismo , Progresión de la Enfermedad , Femenino , Factor 2 de Crecimiento de Fibroblastos/metabolismo , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Resultado del Tratamiento , Factor A de Crecimiento Endotelial Vascular/metabolismoRESUMEN
Colorectal cancer (CRC) is one of the most common cancers of the gastrointestinal tract and the fourth cause of cancers death in the world. Soluble adhesion molecules (CAMs) are thought to have an important role in host defense against carcinogenesis. They are biomarkers of inflammation and indicators of the immune response to tumors. The study included 40 CRC patients without remote metastases and 24 control subjects. Serum concentrations of sE-selectin, sICAM and sVCAM in patients with CRC were investigated by ELISA method. The level of the sCAMs decreased significantly after radical tumor resection. Preoperative serum concentrations of sICAM and sVCAM in CRC patients were significantly higher compared to the control group, whereas there were no differences regarding serum sE-selectin. Serum levels of sE-selectin, sICAM and sVCAM correlated significantly with each other. There was a significant correlation of serum levels of sICAM-1 and sVCAM-1, but not sE-selectin, with TNM stage and lymph node involvement. No significant relationship was found between serum concentrations of sICAM-1, sVCAM-1 and sE-selectin in CRC patients and patients' age or gender. Our findings suggest that an improved understanding of the mechanisms of membrane shedding of sICAM, sVCAM and sE-selectin is required to delineate their role in tumor progression.
Asunto(s)
Neoplasias Colorrectales/sangre , Selectina E/sangre , Molécula 1 de Adhesión Intercelular/sangre , Molécula 1 de Adhesión Celular Vascular/sangre , Adulto , Anciano , Biomarcadores de Tumor/sangre , Neoplasias Colorrectales/patología , Neoplasias Colorrectales/cirugía , Femenino , Humanos , Metástasis Linfática , Masculino , Persona de Mediana EdadRESUMEN
PURPOSE: The aim of the present study was to asses the effect of tumor advancement, differentiation grade and surgery treatment on PDGF- AB level and platelet (PLT) count depending on the site of blood collection. MATERIAL AND METHODS: The study included 38 patients submitted to surgical treatment due to diagnosis of colorectal cancer (CRC) without remote metastasis: G2- 20 patients and group G3- 18 patients. The control group consisted of 24 healthy subjects. In CRC patients the blood samples was collected three times: 1) before surgery, 2) intrasurgically and 3) 90 days after surgery. Serum PDGF- AB concentration was determined by ELISA- Kit reagents. RESULTS: PDGF concentration in all the patients was several times higher than in the control group, irrespective of tumor differentiation grade and the site of blood collection. However the level of PDGF- AB in intraoperatively collected arterial blood and venous blood in group G3 (arterial blood- 379.9+/-12.1 ng/ml; venous blood- 398.4+/-13.2 ng/ml) was significantly higher than in group G2 (arterial blood- 169.4+/-88.6 ng/ml; venous blood- 194.2+/-84.0 ng/ml). No significant differences were observed between venous and arterial blood. No correlation was found between the PLT count and PDGF- AB concentration. CONCLUSION: High blood PDGF- AB concentration in CRC patients but no significant positive correlation observed between the PLT count and PDGF-AB suggest its neoplastic origin beside PLT. Determination of this factor in blood serum may have an important implication in early diagnosis of CRC, which is the second most common malignant neoplasm with high recurrence rates.
Asunto(s)
Adenocarcinoma/sangre , Biomarcadores de Tumor/sangre , Recolección de Muestras de Sangre/métodos , Neoplasias Colorrectales/sangre , Factor de Crecimiento Derivado de Plaquetas/metabolismo , Adenocarcinoma/patología , Adenocarcinoma/cirugía , Adulto , Anciano , Arterias , Neoplasias Colorrectales/patología , Neoplasias Colorrectales/cirugía , Femenino , Humanos , Masculino , Persona de Mediana Edad , VenasRESUMEN
The CD62P receptor and its soluble form sP-selectin is a marker of platelet (PLT) activation, and constitutes a ligand for CD24 antigen of neoplastic cells and tumor stroma components. The aim of the study was to evaluate the relationship dynamics of the percentage of CD62P+ platelets, the level of the receptor expression and the concentration of soluble form of sP-selectin in renal cancer. Examinations were performed before and after nephrectomy in patients with renal cancer (group A - 25, T2N0M0; group B - 27, T2N1-2M0) and in control group (C - 24 subjects). The two groups A and B showed an increased subpopulation of CD62P+ platelets (p<0.01) and elevated sP-selectin concentration (p<0.001) before and after nephrectomy. Although following nephrectomy sP-selectin concentration decreased markedly, it was still higher 3 months after the procedure compared to control group (p<0.05). Following nephrectomy, however, no statistically significant differences were found in the % of CD62P+ platelets and the receptor expression. Greater dynamics of changes before and after nephrectomy in the percentage of CD62P+ platelets (B1:B2 p<0.05) and the receptor expression (B1:B3 p<0.001) was observed in patients with local lymph node involvement (group B) while sP-selectin concentration was similar in both groups. Nephrectomy did not normalize intravascular activation of PLT and TNM had no significant effect on the expression of CD62P and concentration of sP-selectin.
Asunto(s)
Carcinoma de Células Renales/sangre , Neoplasias Renales/sangre , Nefrectomía , Selectina-P/sangre , Adulto , Anciano , Biomarcadores de Tumor/sangre , Plaquetas/metabolismo , Carcinoma de Células Renales/secundario , Carcinoma de Células Renales/cirugía , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Neoplasias Renales/patología , Neoplasias Renales/cirugía , Masculino , Persona de Mediana Edad , Activación Plaquetaria , Recuento de PlaquetasRESUMEN
PURPOSE: Previous in vitro studies have demonstrated that a potent antihypertensive agent ebelactone B inhibits cathepsin A/deamidase activity. The aim of our studies was to assess the effects of this inhibitor on cathepsin A activity in intact platelets and on platelet activation events. MATERIAL AND METHODS: PRP or washed human platelets from healthy volunteers were pre-incubated with different concentrations of ebelactone B (1-10 microM) for 10-60 min. Cathepsin A activity in platelets was assayed colorimetrically using Cbz-Phe-Ala at pH 5.5. Expression of platelet activation markers GpIIb/IIIa and P-selectin on non-activated or agonist-activated platelets (ADP, TRAP) was measured by flow cytometry. RESULTS: Pre-treatment of platelets for up to 60 minutes with 10 mumol/l ebelactone B, that effectively inhibits cathepsin A activity in platelet lysate, did not affect this activity in intact platelets. Exposure of PRP to 10 mumol/l ebelactone B alone, or before platelet activation with ADP or TRAP caused only a small but non-significant increase in P-selectin and GpIIb/IIIa expression on the platelet surface, as demonstrated by flow cytometry analysis. CONCLUSIONS: The lack of cathepsin A inhibition by ebelactone B in intact platelets indicates that this inhibitor does not enter cells. Therefore, a potential antihypertensive significance of this compound may be through the inhibition of cathepsin A/deamidase released from activated or damaged cells. In vitro ebelactone B seems to exert no effect on platelet activation. Further studies are underway to determine whether ebelactone B administration affects platelet activation events in experimental model of hypertension in rats.
Asunto(s)
Antihipertensivos/farmacología , Plaquetas/metabolismo , Catepsina A/metabolismo , Lactonas/farmacología , Activación Plaquetaria/fisiología , HumanosRESUMEN
We studied changes in the expression of P-selectin on the blood platelet plasma membrane. The aim of the study was to determine the influence of renal carcinoma on P-selectin expression associated with changes in platelet morphology. Venous blood was collected from 30 patients with renal carcinoma and from 24 control subjects for cytometric analysis and to evaluate platelet morphology. P-selectin being the CD62P receptor on blood platelets was marked by anti-CD61/62P MoAb, and the results were presented as the percentage of CD62P-positive cells. Changes in the expression of the CD62P on the platelet plasma membrane during activation were investigated by flow cytometry in a comparative study of in vivo activation and in vitro platelet reactivity. Platelet activation reflected by P-selectin expression was higher in the group of patients (4.45 +/-1.96), compared to control (2.48 +/-1.66) (p < 0.05). However, adenosine diphosphate [ADP] -stimulated platelet reactivity in renal cancer patients increased only by 0.24% (p > 0.05), while following activation by thrombin by 0.54% (p < 0.05). Moreover, a higher (4.72 +/-2.02), statistically significant percentage of platelets with P-selectin expression was found in patients with disseminated neoplastic changes in renal parenchyma, compared to patients with a single localized neoplastic lesion (4.17 +/-1.89) (p < 0.05). A statistically significant difference was noted in the platelet count and anisocytosis in renal cancer patients. Renal cancer enhances P-selectin expression. It is due to the presence of intensified thrombinogenesis and other platelet agonists in the blood.
Asunto(s)
Plaquetas/metabolismo , Carcinoma de Células Renales/metabolismo , Neoplasias Renales/metabolismo , Selectina-P/metabolismo , Adulto , Anciano , Plaquetas/patología , Carcinoma de Células Renales/diagnóstico , Carcinoma de Células Renales/patología , Femenino , Humanos , Neoplasias Renales/diagnóstico , Neoplasias Renales/patología , Masculino , Persona de Mediana Edad , Activación Plaquetaria , Recuento de PlaquetasRESUMEN
Many studies report on the increased activity of platelets in patients with heart disease, especially with acute myocardial infarction (MI). The aim of the present study was to evaluate dynamics of beta-thromboglobulin (beta-TG) concentration in patients with MI according to the disease duration and type of treatment. We investigated 29 patients, who were divided into two groups according to the type of treatment. beta-TG concentration was determined using the immunoenzymatic method (ELISA). Blood was taken 1st, 3rd, 5th, 8th and 11th day of infarction. Our results indicate that in the course of myocardial infarction there is a change in platelet activation. The treatment applied affects beta-TG concentration, and the thrombolytic therapy inhibits platelet activation.
Asunto(s)
Infarto del Miocardio/sangre , beta-Tromboglobulina/metabolismo , Aspirina/administración & dosificación , Estudios de Casos y Controles , Femenino , Heparina/administración & dosificación , Humanos , Masculino , Persona de Mediana Edad , Infarto del Miocardio/tratamiento farmacológico , Activación Plaquetaria/efectos de los fármacos , Estreptoquinasa/administración & dosificación , Terapia TrombolíticaRESUMEN
Embolization consists in the occlusion of renal artery and its ramifications, which induces acute renal infarction. Renal artery embolization, causing necrosis, stimulates cellular reactions within and around the neoplasm. The interaction of neoplastic cells with blood platelets is of great importance in the development of neoplastic disease. Neoplastic cells exert a stimulatory effect on blood platelets. During activation, platelets change in shape and secrete certain substances. We conducted this study measuring the phagocytic activity of blood platelets. 45 patients suffering from renal cancer were examined (22 women and 23 men) before and after embolization. Diagnosis was established basing on the patients' history, clinical picture, and ultrasonographic, urographic and angiographic examinations. The study demonstrated that embolization led to an increase in platelet count and decrease of phagocytic index.
Asunto(s)
Embolización Terapéutica , Neoplasias Renales/inmunología , Neoplasias Renales/terapia , Fagocitosis , Activación Plaquetaria/inmunología , Adulto , Anciano , Femenino , Humanos , Neoplasias Renales/diagnóstico , Masculino , Persona de Mediana Edad , Recuento de PlaquetasRESUMEN
The urinary alpha1-microglobulin (alpha1-M) as a marker of proximal tubular damage was measured in 86 children, aged 3/12 to 12 years, with a diagnosis of urinary tract infection (UTI) and fever. All patients had normal glomerular filtration rates (GFR). They were divided into 2 groups: A: with UTI and etiological factor E. coli, B: with UTI and etiological factor Proteus sp. Similar measurements of alpha1-M were obtained for a control group of healthy children. An increased mean level serum alpha1-M was observed in patients with UTI and fever compared to control group (p < 0.001). Urinary alpha1-M as the alpha1-microglobulin/creatinine ratio was higher in both tested group of patients with UTI and fever. Those found in group A1 and B1 before treatment were the highest and statistically significantly elevated after treatment (group A2 and B2: p < 0.001). Our results indicate the usefulness of the urinary alpha-microglobulin/creatinine ratio as a marker of proximal kidney tubule damage in children with E. coli and Proteus sp. infections. Additionally, it seems to be associated with the humoral and cellular immune response.
Asunto(s)
alfa-Globulinas/orina , Enfermedades Renales/etiología , Enfermedades Renales/orina , Túbulos Renales Proximales , Inhibidores de Proteasas/orina , Infecciones Urinarias/complicaciones , Biomarcadores/orina , Niño , Preescolar , Femenino , Humanos , Masculino , Estudios ProspectivosRESUMEN
Much attention has been paid to the role of platelets in the pathogenesis of heart diseases, e.g. ischaemic disease and myocardial infarction. During platelet activation changes appear both in the internal structure and in the membrane. The release reaction is associated with the appearance of CD 62P (P-selectin). The aim of the present study was evaluate CD 62P expression in patients with acute myocardial infarction according to the disease duration and the type of treatment. We study 29 patients with acute myocardial infarction (MI). The patients were divided into two groups: group A--15 patients treated with heparin and aspirin and group B--14 patients treated with streptokinase, heparin and aspirin. The control group consisted of 21 healthy subjects. We indicated that P-selectin expression is influenced by infarction duration and type of treatment. Our study demonstrates that thrombolytic therapy inhibits platelet activation. Patients receiving streptokinase showed lower CD 62P expression than those treated only with heparin and aspirin, both before and after platelet activation with thrombin.
Asunto(s)
Infarto del Miocardio/sangre , Selectina-P/sangre , Activación Plaquetaria , Aspirina/uso terapéutico , Biomarcadores/sangre , Femenino , Citometría de Flujo/métodos , Heparina/uso terapéutico , Humanos , Masculino , Persona de Mediana Edad , Infarto del Miocardio/tratamiento farmacológico , Estreptoquinasa/uso terapéuticoRESUMEN
The aim of the present study was evaluate dynamics of platelet factor 4, as a marker of platelet activation, in patients with acute myocardial infarction according to the disease duration and type of treatment. In the recent years much attention has been paid to the role of platelets in the pathogenesis of ischaemic disease and myocardial infarction. Rupture or splitting of atheroma and increased platelet activation are a direct cause of acute thrombotic process in coronary vessels. During platelet activation alpha granules release proteins, e.g. platelet factor 4 (PF 4). We investigated 29 patients with acute myocardial infarction (MI); the patients were divided into two groups: group A--15 patients treated with heparin and aspirin; group B--14 patients treated with streptokinase, heparin and aspirin. Control group (C) consisted of 21 healthy subjects. PF 4 concentration was determined on the 1st, 3rd, 5th, 8th, 11th day of MI using the immunoenzymatic method. Our results indicate that in the course of myocardial infarction there is a change in the platelet factor 4 and that thrombolytic therapy inhibits platelet activation.
Asunto(s)
Infarto del Miocardio/sangre , Activación Plaquetaria , Factor Plaquetario 4/análisis , Aspirina/uso terapéutico , Factores Biológicos/sangre , Femenino , Heparina/uso terapéutico , Humanos , Masculino , Persona de Mediana Edad , Infarto del Miocardio/tratamiento farmacológico , Estreptoquinasa/uso terapéuticoRESUMEN
Embolization consists in the occlusion of renal artery and its ramifications, which induces acute renal infarction. Renal artery embolization, causing necrosis, stimulates cellular reactions within and around the neoplasm. The interaction of neoplastic cells with blood platelets is of great importance in the development of neoplastic disease. Neoplastic cells exert a stimulatory effect on blood platelets. During activation, platelets change in shape and secrete certain substances. The aim of the present study was to evaluate morphological parameters of blood platelets in renal cancer patients before and after renal artery embolization. 45 patients suffering from renal cancer were examined (22 women and 23 men) before and after embolization. Diagnosis was established basing on the patients' history, clinical picture, and ultrasonographic, urographic and angiographic examinations. Tumour advancement was defined as T2 and T3. The study has demonstrated that embolization leads to an increase in platelet count and in the percentage of "megathrombocytes". The changes observed in the study may indirectly testify to the involvement of the thrombopoietic system in the course of neoplastic disease.
Asunto(s)
Plaquetas/patología , Neoplasias Renales/sangre , Neoplasias Renales/patología , Obstrucción de la Arteria Renal/sangre , Obstrucción de la Arteria Renal/patología , Adulto , Anciano , Femenino , Humanos , Neoplasias Renales/complicaciones , Masculino , Persona de Mediana Edad , Recuento de Plaquetas , Obstrucción de la Arteria Renal/complicacionesRESUMEN
The aim of our present study was to analyse the dynamics of changes in the phagocytic activity of platelets with myocardial infarction according to the treatment applied. Twenty patients with acute myocardial infarction and thirty healthy subjects were examined. Platelet count, phagocytic activity of platelets and the phagocytic index were determined. We observed reduced phagocytic activity of platelets in the first 24 hours of infarction, which may be due to exhaustion of most active platelets in a thrombus and microaggregates. A transitory increase in the phagocytosis activity of platelets observed on the third day of infarction may be caused by the release of large, more active platelets from splenic pool. On the eight day of infarction, the phagocytic activity of platelets became normalized in both experimental groups. Our study indicates that together with platelet activation the phagocytic activity gets reduced being a likely response to the thrombocytopenic factor. This can be confirmed by the increased number of platelets on the 8th day. Our results suggest a slightly attenuated phagocytic ability of platelets, irrespective of the treatment applied. The reduction seems to result from the effect of platelets exhaustion on the procoagulative activity in patients with myocardial infarction.
Asunto(s)
Plaquetas/fisiología , Infarto del Miocardio/fisiopatología , Fagocitosis , Adulto , Humanos , Persona de Mediana Edad , Infarto del Miocardio/tratamiento farmacológico , Factores de TiempoRESUMEN
The aim of the study was to determine the effect of renal tumor embolization on nonspecific immunity by evaluating lysozyme activity and leucocytosis in 45 patients and 40 healthy people. Lysozyme activity was assessed in the non-diluted serum (A1) and in the tenfold diluted serum (B1) prior to embolization and after embolization (A2, B2) and in control group. Prior to embolization, lysozyme activity was lower in the experimental group (A1 and B1), compared to the control groups, the differences being statistically significant (p < 0.05). After embolization, the activity became normalized (A2), reaching the control value and even exceeding it (C) in the diluted serum (B2). Leucocytosis prior to embolization (L1) resembled that of control group, increasing slightly after embolization (L2). The differences observed in the changes in lysozyme activity and leucocytosis were statistically significant (p < 0.05). Our findings indicate an inhibitory effect of the neoplastic process on nonspecific immunity. Embolization causes ischemic necrosis of tumor and products of neoplastic tissue disintegration exert a stimulating effect on granulopoiesis, by increasing the turnover of neutrophilic granulocytes. Granulocytic-monocytic infiltrations in tumor stroma are the source of lysozyme, enhancing not only local but also systemic immunity, which is manifested in the increased lysozyme activity in blood serum.
Asunto(s)
Embolización Terapéutica , Neoplasias Renales/terapia , Muramidasa/sangre , Adulto , Femenino , Humanos , Neoplasias Renales/enzimología , Neoplasias Renales/inmunología , Leucocitosis/inmunología , Masculino , Persona de Mediana EdadRESUMEN
The activity of granulocytic myeloperoxidase (MPO) was investigated in 45 patients with renal cancer before and 14 days after renal artery embolization in comparison with control group of 40 healthy people. The group examined showed lower MPO activity before embolization, as compared to controls. The differences being statistical insignificant (p > 0.05). After embolization the activity MPO was normalized, reaching the mean value of control group. The findings revealed reduced granulocytic MPO activity in patients with renal cancer.
Asunto(s)
Biomarcadores de Tumor/análisis , Embolización Terapéutica , Neoplasias Renales/sangre , Neoplasias Renales/terapia , Neutrófilos/enzimología , Peroxidasa/metabolismo , Adulto , Femenino , Humanos , Neoplasias Renales/patología , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Peroxidasa/análisis , Valores de Referencia , Sensibilidad y EspecificidadRESUMEN
The main role of neutrophilic granulocytes is phagocytosis and destruction of phagocytized bacteria. The nitroblue tetrazolium test (NBT), used to evaluate the bactericidal function of granulocytes, was assessed in 45 patients with renal tumour before and 14 days after renal artery embolization. No statistically significant spontaneous test NBT differences were found, compared with control group both before and 14 days after embolization. Following stimulation granulocytes in vitro by LPS of E. coli the ability of granulocytes to reduce NBT was lowered. The differences, as compared with control group, were statistically significant (p < 0.05). Our results indicates considerable impairment of lower metabolic reserve in patients with renal tumour necessary for maximum stimulation.
Asunto(s)
Adenocarcinoma/diagnóstico , Adenocarcinoma/terapia , Embolización Terapéutica , Granulocitos/química , Indicadores y Reactivos/análisis , Neoplasias Renales/diagnóstico , Neoplasias Renales/terapia , Nitroazul de Tetrazolio/análisis , Adenocarcinoma/sangre , Adulto , Femenino , Humanos , Neoplasias Renales/sangre , Masculino , Persona de Mediana Edad , Valores de Referencia , Arteria Renal , Sensibilidad y EspecificidadRESUMEN
Serum concentration of alpha1-proteinase inhibitor (alpha1-PI) was determined by nephelometric method in forty seven patients with liver cirrhosis (LC) and fifteen patients with hepatocellular carcinoma (HCC). Our data show an increase in the concentration of alpha1-PI in the group with decompensated LC by 17% and in HCC by 29%. The level of alpha1-PI higher than (220 mg%) may be indicative of the disease progression towards decompensated LC or HCC. In the conclusion, an increase in alpha1-PI concentration in patients with LC may be considered as an alarming factor, but is not sufficiently specific to become a diagnostic tool for the detection of HCC development.
Asunto(s)
Carcinoma Hepatocelular/sangre , Cirrosis Hepática/complicaciones , Neoplasias Hepáticas/sangre , Inhibidores de Tripsina/sangre , alfa 1-Antitripsina/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Carcinoma Hepatocelular/etiología , Progresión de la Enfermedad , Femenino , Humanos , Cirrosis Hepática/sangre , Neoplasias Hepáticas/etiología , Masculino , Persona de Mediana Edad , Nefelometría y TurbidimetríaRESUMEN
Ultrastructural evaluation was performed of the MK cytoplasm in the successive phases of experimental haemorrhagic shock in rats. Qualitative abnormalities were revealed in the intracytoplasmic structures of MK. Platelets in the circulating blood were evaluated. A correlation was found between the changes in the intracytoplasmic organelles and the number and biological activity of blood cells. The results obtained indicate significant disturbances in the functioning of the thrombopoietic system in experimental haemorrhagic shock, which are caused by alterations within the intracytoplasmic structures of MK and lead to platelet production from morphologically differentiated but functionally immature MK.
Asunto(s)
Plaquetas/patología , Hematopoyesis , Megacariocitos/ultraestructura , Choque Hemorrágico/patología , Animales , Citoplasma/ultraestructura , Modelos Animales de Enfermedad , Femenino , Microscopía Electrónica , Recuento de Plaquetas , Ratas , Ratas WistarRESUMEN
Immunoturbidimetric method was applied to estimate C-reactive protein concentration in blood serum in 32 women aged 47.8 (+/-6.3) years in whom abdominal hysterectomy had been performed. On the 3rd day after the surgery in 11 (34.4%) patients there occurred inflammatory complications followed by evaluated body temperature (38 degrees C and higher), abdominal wound and/or parametrium inflammatory infiltrations. 21 patients recovered without side effects. In the group of patients in whom on the 3rd day or later infection manifestation appeared, greater difference of CRP concentration was detected in 24th hour after operation. These findings are suggestive of the usefulness of estimating blood serum CRP as en early and preclinical marker of postsurgery inflammation process.
Asunto(s)
Proteína C-Reactiva/análisis , Histerectomía/efectos adversos , Infección de la Herida Quirúrgica/diagnóstico , Adulto , Biomarcadores/sangre , Femenino , Fiebre/etiología , Humanos , Persona de Mediana Edad , Infección de la Herida Quirúrgica/sangre , Infección de la Herida Quirúrgica/etiologíaRESUMEN
Dysmorphic erythrocyturia is known as an attribute of glomerular bleeding. During chronic glomerulonephritis the morphological changes of blood platelets were also observed. The subject of our study was to indicate if the common mechanism leading to blood platelets polymorphology and dysmorphic erythrocytes origination in chronic glomerulonephritis is possible. We estimate the count of blood platelets (PLT), mean platelets volume (MPV), platelet distribution weight (PDW) and platelets crit (PCT) in peripheral blood of 46 patients with chronic glomerulonephritis with dysmorphic erythrocyturia (26 with renal sufficiency and 20 with renal insufficiency) and 32 healthy volunteers. The dysmorphic erythrocyturic erythrocytes were examined in first morning urine. We have not demonstrated the correlation of changes in blood platelets morphology with percent dysmorphic erythrocytes in urine. The results of our investigations do not confirm exactly the common mechanism of the morphological changes which were observed. Minimal changes of the blood platelets parameters may be the results of changes in structure of phospholipids platelets membrane as well as less activation of platelets membrane receptors-GP Ia/IIb and GP Ic/IIa.