RESUMEN
1-->3; 1-->6-beta-D-Glucooligosaccharides with polymerization degree of 5-6 were obtained from laminaran and analyzed by the previously developed method [1] by using them as donors and p-nitrophenyl beta-D-glucoside as the acceptor in the transglycosylation reaction catalysed by endo-1-->3-beta-D-glucanase LIV. The resulting homologous p-nitrophenyl beta-1,3-laminarioligosides with polymerization degree of 2-6 and the corresponding derivatives of mixed beta-1,3; 1,6-glucooligosaccharides with the same polymerization degree were analyzed by HPLC. The latter compounds exhibited higher retention times than the former with the same polymerization degree. Isomeric tetra-, penta-, and hexameric compounds were detected, and some of them were structurally characterized by means of NMR. The suggested method of analysis of oligosaccharide mixtures is simple, informative, and consumes a minimal quantity of sample.
Asunto(s)
Glucano Endo-1,3-beta-D-Glucosidasa/química , Oligosacáridos/química , Secuencia de Carbohidratos , Cromatografía Líquida de Alta Presión , Glucanos , Glicosilación , Hidrólisis , Laminaria/química , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Polímeros , Polisacáridos/químicaRESUMEN
The acidic capsular polysaccharide from the museum strain 2211 of Klebsiella ozaenae serotype K4 is built up of pentasaccharide repeating-units that contain residues of D-glucose, D-mannose, D-glucuronic acid, and 3-deoxy-D-glycero-D-galacto-nonulosonic acid (Kdn) in the ratios 2:1:1:1, as well as an O-acetyl group. The last-named sugar, which is reported in bacterial polysaccharides for the first time, was identified as the methyl (methyl 3-deoxynonulopyranosid)onate obtained by methanolysis of the polysaccharide. On the basis of the results of partial acid hydrolysis, Smith degradation, and computer-assisted 13C-n.m.r. analysis, it was concluded that the capsular polysaccharide has the following structure: (formula see text)
Asunto(s)
Klebsiella/inmunología , Polisacáridos Bacterianos , Azúcares Ácidos/análisis , Conformación de Carbohidratos , Secuencia de Carbohidratos , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Rotación Óptica , Polisacáridos Bacterianos/aislamiento & purificación , SerotipificaciónRESUMEN
3-Deoxy-D-glycero-D-galacto-nonulosonic acid was identified as a component of the Klebsiella ozaenae K4 capsular polysaccharide. On the basis of methylation, complete and partial acid hydrolyses, Smith degradation, and NMR analysis including computer-assisted 13C NMR evaluation, the following structure of the polysaccharide has been established.
Asunto(s)
Klebsiella/inmunología , Polisacáridos Bacterianos/análisis , Azúcares Ácidos/análisis , Secuencia de Carbohidratos , Fenómenos Químicos , Química , Glucuronatos/análisis , Ácido Glucurónico , Hidrólisis , Espectroscopía de Resonancia MagnéticaRESUMEN
Glycosaminoglycans of tegmental cartilage of human caput femoris were separated into the fractions, which were identified as keratosulfate, hyaluronic acid, heparitin sulphate, chondroitin-4-sulphate, chondroitin-6-sulphate, dermatan sulphate and heparin. Content of glycosaminoglycans in articular cartilage of healthy persons and patients with deforming arthrosis was determined. Content of sulphatated polysaccharides (dermatan sulphate, chondroitin-6-sulphate, keratosulfate and heparitin sulphate) was shown to be distinctly decreased in the cartilage of the patients as compared with the amount of the polysaccharides in the same tissue of healthy persons.