RESUMEN
Western lowland gorillas (Gorilla gorilla gorilla) are Critically Endangered and show continued population decline. Consequently, pressure is mounting to better understand their conservation threats and ecology. Gastrointestinal symbionts, such as bacterial and eukaryotic communities, are believed to play vital roles in the physiological landscape of the host. Gorillas host a broad spectrum of eucaryotes, so called parasites, with strongylid nematodes being particularly prevalent. While these communities are partially consistent, they are also shaped by various ecological factors, such as diet or habitat type. To investigate gastrointestinal symbionts of wild western lowland gorillas, we analysed 215 faecal samples from individuals in five distinct localities across the Congo Basin, using high-throughput sequencing techniques. We describe the gut bacterial microbiome and genetic diversity of strongylid communities, including strain-level identification of amplicon sequence variants (ASVs). We identified strongylid ASVs from eight genera and bacterial ASVs from 20 phyla. We compared these communities across localities, with reference to varying environmental factors among populations, finding differences in alpha diversity and community compositions of both gastrointestinal components. Moreover, we also investigated covariation between strongylid nematodes and the bacterial microbiome, finding correlations between strongylid taxa and Prevotellaceae and Rikenellaceae ASVs that were consistent across multiple localities. Our research highlights the complexity of the bacterial microbiome and strongylid communities in several gorilla populations and emphasizes potential interactions between these two symbiont communities. This study provides a framework for ongoing research into strongylid nematode diversity, and their interactions with the bacterial microbiome, among great apes.
Asunto(s)
Microbioma Gastrointestinal , Microbiota , Animales , Bacterias/genética , Bacteroidetes , Heces/microbiología , Microbioma Gastrointestinal/genética , Gorilla gorilla/genética , HumanosRESUMEN
Accurate measures of animal population densities are essential to evaluate conservation status and implement action plans to ensure species survival. Gorilla numbers were assessed using the recce survey method within Moukalaba-Doudou National Park (MDNP) in Gabon using fresh nest counts of up to 1 week old. We walked 3,592 km within a 23.01-km2 study site totalling a sampling effort of 297 days. Encounter rate was 0.12 fresh nests per kilometre, and gorilla density estimates generated by home range sizes (by minimal convex polygon) ranged between 1.14 and 1.48 gorillas/km2. Gorillas preferred mixed forest for nesting over other habitats (Cheason index value 1.31). Results showed that gorilla density values within the study area concurred with previous studies that used line transect methodologies. We conclude that the choice of sampling design is dependent on environmental conditions characterised by each habitat type and target species.
Asunto(s)
Gorilla gorilla , Densidad de Población , Animales , Ecosistema , Gabón , Fenómenos de Retorno al Lugar Habitual , Comportamiento de NidificaciónRESUMEN
BACKGROUND AND AIM: Gastrointestinal parasites identified in the wild can negatively affect host fitness, lower performance, and growth. On the other side, sympatric mammals that share habitat and resources may also cross-transmit parasites, which are often zoonotic and can contribute to morbidity and mortality. This study aimed to characterize the diversity of gastrointestinal parasites circulating in mammalian hosts in Moukalaba-Doudou National Park. MATERIALS AND METHODS: We screened a total of 25 fecal samples collected from nine wild mammalian species, namely, western gorilla (Gorilla gorilla gorilla), chimpanzee (Pan troglodytes), putty-nosed monkey (Cercopithecus nictitans), African forest elephant (Loxodonta cyclotis), African buffalo (Syncerus caffer), blue duiker (Philantomba monticola), bay duiker (Cephalophus dorsalis), and red river hog (Potamochoerus porcus) as well as people working as trackers (Homo sapiens) using direct microscopic observations following a sedimentation technique to concentrate the fecal material. RESULTS: Of the total 25 fecal samples screened, 15 (60%) were positive for parasitic gastrointestinal infection. Based on the morphology of parasite eggs and cysts, we identified a rich diversity of nematodes, protozoans, trematodes, and cestodes, including unidentified strongyles (73%), Oesophagostomum spp. (53%), Ancylostoma spp. (27%), Trichuris spp. (13%), Ascaris spp. (13%), Mammomonogamus spp. (13%), Strongyloides spp. (47%), Balantidium coli (20%), Entamoeba coli (20%), Endolimax nana (6%), Fasciola hepatica (6%), Paramphistomum spp. (13%), and Taenia spp. (6%). CONCLUSION: All parasites were found at least once in one of the hosts, and most were potentially zoonotic and responsible for several diseases of public health concern. Because of the small sample size, our findings should not be considered conclusive. Nevertheless, they highlight the diversity of gastrointestinal parasites in this area.
RESUMEN
In our most recent study, we found that in Tanzania infection with Treponema pallidum (TP) subsp. pertenue (TPE) is present in four different monkey species. In order to gain information on the diversity and epidemiological spread of the infection in Tanzanian nonhuman primates (NHP), we identified two suitable candidate genes for multi-locus sequence typing (MLST). We demonstrate the functionality of the MLST system in invasively and non-invasively collected samples. While we were not able to demonstrate frequent interspecies transmission of TPE in Tanzanian monkeys, our results show a clustering of TPE strains according to geography and not host species, which is suggestive for rare transmission events between different NHP species. In addition to the geographic stability, we describe the relative temporal stability of the strains infecting NHPs and identified multi-strain infection. Differences between TPE strains of NHP and human origin are highlighted. Our results show that antibiotic resistance does not occur in Tanzanian TPE strains of NHP origin.
Asunto(s)
Cercopithecus/microbiología , Chlorocebus aethiops/microbiología , Especificidad del Huésped , Enfermedades de los Monos/transmisión , Papio anubis/microbiología , Papio cynocephalus/microbiología , Treponema/clasificación , Infecciones por Treponema/veterinaria , Animales , Enfermedades del Simio Antropoideo/epidemiología , Enfermedades del Simio Antropoideo/microbiología , Enfermedades del Simio Antropoideo/transmisión , Congo/epidemiología , Heces/microbiología , Estudios de Asociación Genética , Variación Genética , Gorilla gorilla/microbiología , Enfermedades de los Monos/epidemiología , Enfermedades de los Monos/microbiología , Tipificación de Secuencias Multilocus , Filogenia , Polimorfismo de Nucleótido Simple , Especificidad de la Especie , Tanzanía/epidemiología , Treponema/genética , Treponema/aislamiento & purificación , Infecciones por Treponema/epidemiología , Infecciones por Treponema/microbiología , Infecciones por Treponema/transmisiónRESUMEN
Findings of regional variations in the behavioral patterns of non-human primates have led to the vigorous study of animal traditions (or culture), which contribute to a biological understanding of diversity in human cultures. Although our knowledge of behavioral variations of the bonobo (Pan paniscus) is limited compared with its sister species, the chimpanzee (P. troglodytes), variations in the prey of this species have been reported across study sites. This study describes evidence of mammals consumed by bonobos in the Iyondji site, which was established in 2010. We found evidence that Iyondji bonobos consumed duikers (Cephalophus dorsalis, C. monticola) and diurnal monkeys (Cercopithecus ascanius), which is notable because only anomalures (Anomalurus spp.) are consumed by bonobos in Wamba, a long-term study site established in 1973, located in an area adjacent to Iyondji. Moreover, bonobos do not transfer between the two populations due to the river between the sites. According to our census of duikers and diurnal monkeys, Iyondji bonobos appeared to encounter diurnal monkeys more frequently than did Wamba bonobos. Although humans have apparently had a more pronounced impact on the habitats in Wamba than on those in Iyondji, it remains unclear how such environmental conditions may have contributed to the differences in the prey consumed by bonobos in different sites. Our findings suggest that additional research at various sites could reveal the nature of the variations in the behavior of bonobos.