RESUMEN
In the present investigation we purpose a simple, rapid and accurate method for the evaluation of sodium nitroprusside. In alkaline medium and on the presence of thiourea, a new complex is formed. The acidification of medium induced a blue solution (lambda m = 600 nm) that could be determined by spectrophotometry.
Asunto(s)
Nitroprusiato/análisis , ColorimetríaRESUMEN
A distribution study was carried out on Cynomolgus monkeys (Macaca fascicularis) dosed orally with 14C-labelled N-N'-dicyclopropyl-methyl-piperazine-dichlor-hydrate 14C-Ino 2628-CZ and sacrificed 2, 8 and 24 h after dosage, using whole-body autoradiography. It is demonstrated that radioactivity is found in all the body; high intakes occur in melanin containing tissues, in blood-forming organs, and in accessory genital glands. Moreover, radioactivity crosses the blood-brain barrier and is mainly localized in hippocampus, caudate, putamen and frontal cortex.
Asunto(s)
Cardiotónicos/farmacocinética , Piperazinas/farmacocinética , Administración Oral , Animales , Autorradiografía , Cardiotónicos/administración & dosificación , Macaca fascicularis , Piperazinas/administración & dosificación , Distribución TisularRESUMEN
The distribution of radioactivity in rats after intravenous or oral administration of 14C-labelled N-N'-dicyclopropyl-methyl-piperazine-dichlorhydrate (14C-Ino 2628-CZ) demonstrated that the labelled compound was widely distributed in the body. An important diffusion of the isotope was found in the central nervous system, the gastric mucosa and several glands. Only small amounts of radioactivity were found in these organs 24 h after dosage.
Asunto(s)
Cardiotónicos/farmacocinética , Piperazinas/farmacocinética , Animales , Autorradiografía , Cardiotónicos/administración & dosificación , Cromatografía en Capa Delgada , Femenino , Piperazinas/administración & dosificación , Ratas , Ratas Sprague-Dawley , Distribución TisularRESUMEN
N-N'-Dicyclopropyl-methyl-piperazine-dichlorhydrate (Ino 2628-CZ) is a novel inotropic compound. The absorption, distribution, excretion and metabolism of radiochemically labelled 14C-Ino 2628-CZ has been studied in male and female rats after intravenous and oral administration. The compound was mainly excreted during the first 24 h after both administrations, although traces of radioactivity were still measured at 7 days post-dose. About 20% of the drug is excreted in the urine unchanged. Nine metabolites were separated, four of them being characterized.
Asunto(s)
Cardiotónicos/farmacocinética , Piperazinas/farmacocinética , Administración Oral , Animales , Cardiotónicos/administración & dosificación , Cardiotónicos/sangre , Heces/química , Femenino , Inyecciones Intravenosas , Absorción Intestinal , Masculino , Piperazinas/administración & dosificación , Piperazinas/sangre , Ratas , Ratas Sprague-DawleyRESUMEN
Carbon monoxide CO, generated from chloral (10(-2) M) in a dilution of hemochrome (pH 13), turns the solution of hemochrome into a solution of carboxyhemoglobin. The spectrum of this solution is identical to the one obtained after bubbling with CO. The reaction is fast and makes it possible to determine the percentage of carboxyhemoglobinemia in whole blood.