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Canine dirofilariasis, caused by Dirofilaria immitis, is prevalent worldwide. However, the frequency of canine infection in the state of Alagoas, Brazil is scarcely unknown. This study aimed to evaluate the frequency of D. immitis infection in dogs from the coastal municipalities of Alagoas and analyze the risk factors associated with the infection. A cross-sectional study was performed using 426 dogs of different breeds and sex distributed across 15 coastal municipalities in the state of Alagoas. Blood samples were collected from each dog and analyzed for circulating microfilariae and free D. immitis antigens. To investigate the risk factors associated with D. immitis infection, we collected information on dog environments using an epidemiological questionnaire. The results revealed that 12.7% of dogs tested positive for D. immitis. Dogs with travel history were 3.52 times more likely to be infected. Thus, infected dogs in the coastal region of Alagoas should be regularly monitored and the public health system should plan strategies to control this zoonotic disease.

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In vitro embryos production from prepubertal heifers can help contribute to breeding programs; however, strategies are necessary to increase their embryo production. The aim of this study was to investigate the effects of two nutritional plans on oocyte recovery, embryo production and growth performance of prepubertal Nelore heifers. Thirty-four Nelore heifers with age of 6.5 months were divided into two feeding treatments (NP1 and NP2). The NP1 diets served as the control and NP2 diets were formulated to contain an average of 1.22-fold more energy than NP1. After 3 months of supplementation, the animals underwent follicular aspiration (ovum pick-up, OPU) every 21 d for 3 months and embryos were produced in vitro. Wither height, chest depth, body weight and subcutaneous fat of animals were measured. The number of retrieved and viable oocytes per OPU were 1.49-fold and 1.42-fold greater in NP2 heifers (p = 0.018 and p = 0.049, respectively) than those in NP1 heifers. Heifers administered NP2 produced 29.7% blastocysts, a percentage higher than NP1 animals that produced 24.40% embryos (p < 0.05). Consequently, females in the NP2 treatment showed improved body development. These results indicate a positive effect of a higher energy diet on assisted reproduction and body development in prepubertal heifers.

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Gliricidia (Gliricidia sepium) is a tree legume that has great potential for use in agriculture because of its multiple-use characteristics. However, there is little information in the literature about the effect of agrisilvicultural systems on nitrogen (N) cycling. This study evaluated the effect of densities of gliricidia on N cycling under an agrisilvicultural system. The treatments were composed of different densities of gliricidia: 667, 1000 and 1333 plants ha-1, with a fixed spacing of 5 m between the alleys. The efficiency of N use was investigated by using the 15N isotope tracer. In each plot, a transect perpendicular to the tree rows was established in two positions: (i) in the corn (Zea mays) row adjacent to the trees, and (ii) in the corn row in the center of the alley. The N fertilizer recovery efficiency ranged from 39% in the density of 667 plants ha-1 to 89% with 1000 plants ha-1. The effect of gliricidia on the N uptake by corn was higher in the central position of the alley with 1000 plants ha-1. The agrisilvicultural system with 1000 plants ha-1 was highly efficient in the recovery of mineral N, representing an excellent option for integrated production systems in tropical regions.

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Coffee farmers have faced problems due to drought periods, with irrigation being necessary. In this sense, this study aimed to evaluate the responses to different levels and durations of water deficit in arabica coffee genotypes in the Cerrado region. The experiment consisted of three Coffea arabica genotypes and five water regimes: full irrigation (FI 100 and FI 50-full irrigation with 100% and 50% replacement of evapotranspiration, respectively), water deficit (WD 100 and WD 50-water deficit from June to September, with 100% and 50% replacement of evapotranspiration, respectively) and rainfed (without irrigation). The variables evaluated were gas exchange, relative water content (RWC) and productivity. The results showed that during stress, plants under the FI water regime showed higher gas exchange and RWC, differently from what occurred in the WD and rainfed treatments; however, after irrigation, coffee plants under WDs regained their photosynthetic potential. Rainfed and WD 50 plants had more than 50% reduction in RWC compared to FIs. The Iapar 59 cultivar was the most productive genotype and the E237 the lowest. Most importantly, under rainfed conditions, the plants showed lower physiological and productive potential, indicating the importance of irrigation in Coffea arabica in the Brazilian Cerrado.

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Enzyme activities (EAs) and the FERTBIO sample concept have been increasingly adopted as a novel approach to estimate the soil quality in Brazil. However, the performance of this strategy in sandy soils of the Cerrado biome remains unclear. During 2 years, in a Cerrado's sandy soil, the short-term effects of ten different cropping systems (conventional tillage or no-tillage associated with monoculture, rotations, and/or successions) on the activities of ß-glucosidase, acid phosphatase, and arylsulfatase were studied. Issues related to annual variability and the feasibility of using the FERTBIO sample concept for soil enzymes activities were also evaluated. Soil samples were collected at three different depths (0-10 cm, 10-20 cm, and 20-40 cm) in March 2017 and February 2018. Five years since the beginning of the experiment, the presence of cover crops and no-till promoted improvements in EAs evidencing the importance of regenerative management practices for the sustainability of agroecosystems in sandy soils. Regardless of the cropping systems and depths evaluated, soil organic carbon and EAs showed low temporal variation during the 2 years of monitoring. Our results also showed that it is possible to use the FERTBIO sample concept for the Quartzipsament soils of Western Bahia, Brazil.

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The first detection of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) in Brazil pointed to a gloomy future for South American agriculture in the 2012/2013 harvest, and insecticide sales increased exponentially during 2013 and 2014. However, after this outbreak, the population of this insect returned to manageable levels. This study documents data on H. armigera moth collection using light traps during the outbreak in two locations: (a) western region of the state of Bahia, including population levels of H. armigera and main noctuid pests during the big outbreak (February and March 2013); (b) Federal District, including monthly data for five growing seasons (July 2012-June 2017). During the outbreak, an average of 527.3 moths were collected per trap (cotton crop, March) in the western region of Bahia each night, while the maximum average in the Federal District was 75.4 moths (January). The number of moths decreased in the following growing seasons, and in October 2016, the maximum number was 2.8 moths per trap. Aspects related to the variations of H. armigera and Helicoverpa zea (Boddie) populations, including the study of phenology and relationship with climatic factors and Oceanic Niño Index, were analyzed and discussed. All aspects are approached to contribute to the management of lepidopteran pests, especially H. armigera, in the productive systems of the Brazilian Cerrado.

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ABSTRACT: Sweet cassava breeding programs are focused on the development of bio fortified cultivars that combine significant amounts of carotenoids in their reserve roots with desirable agronomic. The objective of this research was to evaluate agronomic and biochemical traits in sweet cassava clones with roots that have pink pulp. The nine genotypes were evaluated in two seasons in a randomized block design with three replications. Among the evaluated clones, the following stood out: i) for the height of the first branch (390/08, 345/08 and the control IAC 576-70); ii) for plant height (390/08, 345/08 e 378/08); iii) for shoot weight without original steam cutting (390/08, 406/08, 378/08 e 341/08); iv) for the percentage of starch in roots (378/08, 413/08, 390/08 and the control IAC 576-70); and v) for the root yield (the control IAC 576-70 and 341/08, 390/08, 406/08 e 387/08). In the 2011/2012 season, all clones cooked within 30 minutes, indicating that they all have good culinary qualities. Regarding the total carotenoid content in the roots, the clones that stood out were 406/08 and 341/08. All clones evaluated had HCN content below 100 mg kg-1. Clones 341/08 and 406/08 have agronomic and biochemical potential for direct cultivation by producers in the Cerrado region of Central Brazil and / or for use as stock in sweet cassava breeding programs.

RESUMO: Os programas de melhoramento genético de mandioca de mesa estão focados no desenvolvimento de variedades biofortificadas que aliem aos caracteres agronômicos a presença de carotenoides nas raízes de reserva. Neste trabalho, objetivou-se avaliar características agronômicas e bioquímicas em clones de mandioca de mesa com polpa rosada. Os nove genótipos foram avaliados por duas safras em delineamento experimental de blocos casualizados com três repetições. Dentre os clones avaliados se destacaram: i) para altura da primeira ramificação (390/08, 345/08 e a testemunha IAC 576-70); ii) para altura da planta (390/08, 345/08 e 378/08); iii) para peso da parte aérea sem a cepa (390/08, 406/08, 378/08 e 341/08); iv) para porcentagem de amido nas raízes (378/08, 413/08, 390/08 e a testemunha IAC 576-70); v) para produtividade de raízes (IAC 576-70 e 341/08, 390/08, 406/08 e 387/08). Na safra 2011/2012, todos os clones cozinharam em até 30 minutos, indicando que todos apresentam boas qualidades culinárias. Com relação ao teor de carotenoides totais nas raízes, os clones que se destacaram foram 406/08 e 341/08. Todos os clones avaliados apresentaram teores de HCN nas raízes de reserva de mandioca, inferiores a 100 mg kg-1. Os clones 341/08 e 406/08 apresentam potencial agronômico e bioquímico para cultivo direto pelos produtores na região do Cerrado do Brasil Central e/ou para a utilização como genitores em programas de melhoramento genético de mandioca de mesa.

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ABSTRACT The owlet moths (Lepidoptera: Noctuidae) Anicla infecta (Ochsenheimer 1816), Elaphria agrotina (Guenée 1852) and Spodoptera frugiperda (J.E. Smith 1797) occur in the entire American continent. These polyphagous moths have a preference for grasses, and have different biological habits. In this study, the populations of these three species were evaluated monthly with light traps in the Brazilian Savannah, ranging a span of four crop seasons (from July, 2013 to June, 2017). The population data were analyzed and correlated with the meteorological variables: maximum temperature, minimum temperature, relative humidity and precipitation. A total of 4719 individuals were collected in the following percentages: A. infecta (n = 459; 9.73%), E. agrotina (n = 1809; 38.33%) and S. frugiperda (n = 2451; (51.94%). The abundance of all species went down from the first crop season (2013/2014) to the third (2015/2016). In the fourth crop season (2016/2017), the populations of A. infecta and E. agrotina stabilized, but the abundance of S. frugiperda experienced further decrease. The numbers of individuals of three species declined when precipitation was much above (crop season 2014/2015) and below (crop season 2015/2016) than expected by the climatological normal. There were significant, but different degrees of correlation, between the meteorological factors and the ONI index (Oceanic Niño Index - indicator for monitoring El Niño-Southern Oscillation or "ENSO") with respect to monthly population variations. The results are discussed in accordance with principles of the Integrated Pest Management (IPM) in mind, given the continental distribution and agricultural importance of the three owlet moth species studied.

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The management system of soils and nitrogen application can cause impacts on the N2O emissions produced by the agricultural sector. In the establishment of practices of greenhouse gas mitigation for this sector, the objective of this study was to evaluate the effect of soil tillage, with and without N fertilization, on N2O emissions from Oxisols under rainfed maize in the Brazilian Cerrado region. The managements were of monoculture maize under conventional tillage (CT) and no-tillage (NT), with (1) and without (0) application of N fertilizer (0 and 257 kg N ha-1). From November 2014 to October 2015, gas emissions were measured. The soil and climate variables were measured and related to the N2O fluxes. In the N-fertilized treatments, N2O fluxes were higher (P < 0.01), ranging from -21 µg m-2 h-1 to 548 µg m-2 h-1 N2O under conventional tillage and from -21 µg m-2 h-1 to 380 µg m-2 h-1 N2O under no-tillage, compared with -6 to 93 µg m-2 h-1 N2O from systems without N application. There was a combined effect of mineral N and water-filled pore space for most N2O fluxes. The emission factors of N2O during maize cultivation were lower than the standard factor (1%) established by the International Panel of Climate Change. During the plant crop cycle, 30% less N2O was emitted from the N-fertilized no-tillage than from the conventional tillage. For the total cumulative N2O (crop cycle + fallow), the N2O emissions from NT1 and CT1 were not different, but 10× higher than those from the respective crops without N fertilization. To the emissions per unit of grain yield, CT1 and NT1 emitted 769 and 391 mg N2O kg-1 grain produced, respectively, and NT1 was most efficient in fertilizer-into-product conversion. Under maize cultivation, the soil acted as N2O source, regardless of the management.

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The aim of this work was to investigate the methylation and hydroxymethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and to verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 hr in embryo culture. Expression of HDAC1, HDAC3 and KAT2A genes was measured in embryos by qRT-PCR. Methylation results showed difference between animals, with MSC from BOV2 demonstrating lower methylation rate than BOV1. Meanwhile, MSC-AF were less hydroxymethylated for both animals. MSC-AF from BOV2 produced 44.92 ± 8.88% of blastocysts when embryos were exposed to TSA and similar to embryo rate of MSC-AT also treated with TSA (37.96 ± 15.80%). However, when methylation was lower in FIB compared to MSC, as found in BOV1, the use of TSA was not sufficient to increase embryo production. MSC-AF embryos expressed less HDAC3 when treated with TSA, and expression of KAT2A was higher in embryos produced with all MSC and treated with TSA than embryos produced with FIB. The use of MSC less methylated and more hydroxymethylated in combination with embryo incubation with TSA can induce lower expression of HDAC3 and higher expression of KAT2A in the embryos and consequently improve bovine embryo production.

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In the Brazilian Cerrado, despite the increasing adoption of no-till systems, there are still extended areas under conventional soil management systems that reduce soil carbon (C) and nitrogen (N) stocks and increase the emissions of greenhouse gases, such as nitrous oxide (N2O). Conservation agroecosystems, such as no-till, have been proposed as a strategy to mitigate agriculture-induced climatic changes through reductions in N2O emissions. However, the relationship between organic matter and N2O emissions from soils under different agroecosystems is not yet clear. This study hypothesized that agroecosystems under no-till promote an accumulation of labile and stable SOM fractions along with a reduction of N2O emissions. This study evaluated the effects of crop-rotation agroecosystems: i) on C and N pools and labile and stable SOM fractions; ii) on cumulative N2O emissions; and iii) on the relationships between SOM fractions and N2O emissions. The agricultural systems consisted of: (I) soybean followed by sorghum under no-tillage (NT1); (II) maize followed by pigeon pea under no-tillage (NT2); (III) soybean under conventional tillage followed by fallow soil (CT); (IV) and native Cerrado (CER). After CT for 18years, following the replacement of CER, the soil C stock in the 0-20cm layer was reduced by 0.64tha-1year-1. The no-till systems were more efficient in accumulating labile and stable C fractions with values close to those observed under CER, and were directly related to lower soil N2O emissions. The cumulative pattern of N2O emissions was inverse to that of the following SOM fractions: microbial biomass carbon, permanganate-oxidizable carbon, particulate organic carbon, inert carbon, and humic substances. Based on principal component analysis, the CT was generally separated from the other land use systems. This separation was strongly influenced by the low C contents in the different SOM fractions and higher N2O emissions promoted by the CT.

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Mining to obtain ferronickel by the pyrometallurgical process involves a calcination stage that generates a highly metallic solid dust. This study evaluated the bioavailability of metals from calcination dust after being ingested by Oreochromis niloticus. Chemical analysis was performed on the whole body and histological analysis was performed on the gills and gastrointestinal tract of fish to evaluate potential bioaccumulation. After 96 h of exposure to the dust, gill tissue contained a small amount of particulate matter, while the gastrointestinal tract was filled with the material, generating high values of metals in the fish whole body chemical analysis. However, after 15 and 30 days in clean water, both the gills and gastrointestinal tract were nearly devoid of particles and metal concentrations in the fish whole body had largely returned to baseline levels.

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BACKGROUND: This work presents the results of an epidemiological survey about the professional experience of Speech-Language Pathologists and Audiologists of Rio de Janeiro (Brazil) with children and adolescents who are victims of domestic violence. AIM: To understand the occurrence of child abuse and neglect of children and adolescents treated by speech-language pathologists, characterizing the victims according to: most affected age group, gender, form of violence, aggressor, most frequent speech-language complaint, how the abuse was identified and follow-up. METHOD: 500 self-administered mail surveys were sent to a random sample of professional living in Rio de Janeiro. The survey forms were identified only by numbers to assure anonymity. RESULTS: 224 completed surveys were mailed back. 54 respondents indicated exposure to at least one incident of abuse. The majority of victims were children, the main abuser was the mother, and physical violence was the most frequent form of abuse. The main speech disorder was late language development. In most cases, the victim himself told the therapist about the abuse--through verbal expression or other means of expression such as drawings, story telling, dramatizing or playing. As the majority of the victims abandoned speech-language therapy, it was not possible to follow-up the cases. CONCLUSION: Due to the importance if this issue and the limited Brazilian literature about Speech-Language and Hearing Sciences and child abuse, it is paramount to invest in the training of speech-language pathologists. It is the duty of speech-language pathologists to expose this complex problem and to give voice to children who are victims of violence, understanding that behind a speech-language complaint there might be a cry for help.

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TEMA: este trabalho apresenta os resultados de um inquérito epidemiológico realizado com fonoaudiólogos da cidade do Rio de Janeiro (Brasil) acerca de sua experiência profissional com crianças e adolescentes vítimas de violência familiar. OBJETIVO: conhecer a ocorrência dos maus-tratos na clientela atendida por esses profissionais, caracterizando as vítimas quanto a faixa etária mais atingida, sexo, tipo de violência sofrida, agressor e tipo de queixa fonoaudiológica mais freqüente, além de conhecer como a violência foi identificada e qual foi a evolução dos casos. MÉTODO: foram enviados 500 questionários auto-administráveis por correio para uma amostra aleatória de fonoaudiólogos do Rio de Janeiro. Os questionários retornados foram identificados somente por números para garantir o anonimato. RESULTADOS: dos 224 fonoaudiólogos que responderam ao questionário, 54 atenderam pelo menos um caso de mau-trato, sendo a maioria composta por crianças. O principal agressor foi a mãe e o tipo de violência mais identificada foi a física. O atraso de linguagem foi a queixa fonoaudiológica mais freqüente nas vítimas de violência e a principal forma de identificação foi o relato da própria vítima ao profissional - relato verbal ou ainda, por meio de desenhos, estórias contadas, dramatizações e brincadeiras. Quanto a evolução, a maioria abandonou o tratamento fonoaudiológico impossibilitando o acompanhamento dos casos. CONCLUSAO: dada a importância do tema para a área e a escassez de trabalhos existentes no Brasil sobre Fonoaudiologia e maus-tratos infantis, é imprescindível investir no trabalho de formação e informação deste profissional. É tarefa ainda do fonoaudiólogo, dar visibilidade a um problema tão complexo e principalmente, "dar voz" às crianças vítimas de violência, compreendendo que por trás de uma queixa fonoaudiológica pode haver um pedido de socorro.

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This study analyzes suicide among young Brazilians (15-24 years old) in nine metropolitan areas. Mortality data for 1979-1998 were obtained from the Mortality Information System of the Ministry of Health. External causes are the main causes of death among youth, and suicide is the sixth most frequent of these causes. The distribution is heterogeneous, varying according to the social stratum, specific age group, sex, and means used to commit suicide. All cities analyzed showed increased suicide rates from 1979 to 1998 (from 3.5 to 5.0 per 100,000 inhabitants 15-24 years old). Salvador and Rio de Janeiro had the lowest suicide rates, while Porto Alegre and Curitiba had the highest. The principal means used by youth to commit suicide were hanging, strangling, and suffocation (Porto Alegre), followed by firearms and explosives (Belo Horizonte).

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This study analyzes suicide among young Brazilians (15-24 years old) in nine metropolitan areas. Mortality data for 1979-1998 were obtained from the Mortality Information System of the Ministry of Health. External causes are the main causes of death among youth, and suicide is the sixth most frequent of these causes. The distribution is heterogeneous, varying according to the social stratum, specific age group, sex, and means used to commit suicide. All cities analyzed showed increased suicide rates from 1979 to 1998 (from 3.5 to 5.0 per 100,000 inhabitants 15-24 years old). Salvador and Rio de Janeiro had the lowest suicide rates, while Porto Alegre and Curitiba had the highest. The principal means used by youth to commit suicide were hanging, strangling, and suffocation (Porto Alegre), followed by firearms and explosives (Belo Horizonte).

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