RESUMEN
Nine cleft lip and palate patients aged 17-32 years were included in the study, all having significant maxilla alveolar bone defects. Oral rehabilitation was possible by means of bone augmentation with iliac crest graft, soft tissue plasty and subsequent dental implantation (3 to 6 months after first-step procedures).
Asunto(s)
Proceso Alveolar/anomalías , Proceso Alveolar/cirugía , Labio Leporino/cirugía , Fisura del Paladar/cirugía , Arco Dental/anomalías , Arco Dental/cirugía , Implantación Dental , Ilion/trasplante , Adolescente , Adulto , Femenino , Humanos , Masculino , Adulto JovenRESUMEN
In experiment on 16 grown-up chinchilla rabbits the dynamic of reparative regeneration was evaluated by digital microfocal rontgenography in the terms of 1, 2 and 4 months. Bone defect of the 8capital CHE, Cyrillic8 mm size in the region of mandible angle was caused by surgical laser Smart 2940 D+ on the right side and by physiodespenser Surgec XT on the left side. Surgical laser use let to reduce intact mother bone traumatisation and to improve remote results of bone tissue regeneration. After bone defect creation bone tissue regeneration was put into effect by all 3 callus types - endosteal, periosteal and intermediary.
Asunto(s)
Regeneración Ósea , Fracturas Mandibulares , Tomografía Computarizada por Rayos X/métodos , Animales , Callo Óseo , Chinchilla , Estudios de Seguimiento , Rayos Láser , Fracturas Mandibulares/diagnóstico por imagen , Fracturas Mandibulares/etiología , Factores de TiempoRESUMEN
In experiment on rabbits by histomorphometric method it was shown that after the use during 4 months of titanium plate with the culture of marrow mesenchymal stromal cells there happened full infill of mandible defect by lamellar bone tissue. In control group (titanium plate with inactivated culture of cells) the defect was replaced by regenerate, the most part of it was rough fibrous connective tissue. In experimental group in the central parts of the defect regenerate were characterized by bigger weight of lamellar bone matrix if compared with the peripheral parts of the defect that testified to higher level of its differentiation. Use of stem cells on titanium carrier led in 4 months experiment to full infill of jaw defect by lamellar bone tissue.
Asunto(s)
Regeneración Ósea , Mandíbula/cirugía , Trasplante de Células Madre Mesenquimatosas , Células Madre Mesenquimatosas/citología , Animales , Matriz Ósea , Células Cultivadas , Interpretación Estadística de Datos , Mandíbula/citología , Conejos , Factores de Tiempo , TitanioRESUMEN
Aim of the study was substantiation in experiments on rabbit ramus mandibulae of the method of mesenchymal stem cells (MSC) on the carrier from titanium and gold alloy for reparative osteogenesis enforcement. There was shown stimulating action of the MSC implantation in the bone wound upon reparative osteogenesis at the terms from 1 to 4 months. It was concluded that implants from titanium and gold alloy may be used as carrier for MSC for bone tissue reparative regeneration stimulation.
Asunto(s)
Aleaciones Dentales/uso terapéutico , Oro/uso terapéutico , Mandíbula/cirugía , Traumatismos Mandibulares/cirugía , Trasplante de Células Madre Mesenquimatosas , Titanio/uso terapéutico , Animales , Ingeniería Biomédica , Regeneración Ósea , Mandíbula/patología , Mandíbula/fisiología , Traumatismos Mandibulares/patología , Traumatismos Mandibulares/fisiopatología , RatasRESUMEN
Digital microfocal x-ray study was experimentally studied in animals to examine the time course of changes in their bone regeneration. Sixteen Chinchila rabbits whose bone defect in the angle of the mandibular ramus had been closed with the osteoplastic material Gapcol with the applied allogeneic, autologous stem cells isolated from rabbit adipose tissue and human plasma enriched with thrombocytic growth factors were examined. The capabilities of digital microfocal x-ray study versus x-ray computed tomography were compared in the evaluation of reparative regeneration of bone tissue. The results of radiation studies were verified with the data of scanning electron microscopy.
Asunto(s)
Regeneración Ósea , Huesos/diagnóstico por imagen , Huesos/fisiología , Intensificación de Imagen Radiográfica/métodos , Animales , Humanos , Conejos , Tomografía Computarizada por Rayos XAsunto(s)
Regeneración Ósea , Materiales Biocompatibles Revestidos , Mesodermo/citología , Animales , Células de la Médula Ósea , Materiales Biocompatibles Revestidos/química , Humanos , Implantes Experimentales , Maxilares/lesiones , Ensayo de Materiales , Nylons/química , Procedimientos Quirúrgicos Ortognáticos , Conejos , Células del Estroma , Ingeniería de Tejidos/métodosRESUMEN
Three groups of biostable composite materials were studied. The initial binder polymers (polymethylmethacrylate, polyamide-12, superhigh-molecular-weight polyethylene) and hydroxyapatite-containing composites on the basis of these polymers were tested. Biostable polymers, including those containing hydroxyapatite, were nontoxic for fibroblasts and mesenchymal stem cells: the adhesion parameters for these cells were maximum for polyamide-12 and superhigh-molecular-weight polyethylene and did not depend on the presence of hydroxyapatite. Cell adhesion to "pure" polymethylmethacrylate was significantly lower than to other composites, but increased after integration of hydroxyapatite. The efficiency of proliferation of fibroblast and mesenchymal stem cell on the surface of polyamide-12 and superhigh-molecular-weight polyethylene was maximum and did not depend on the presence of hydroxyapatite. The efficiency of cell proliferation on the surface of "pure" polymethylmethacrylate was low, but increased significantly if it was combined with hydroxyapatite, particularly in areas of mineral particles accumulation. It seems that the presence of high amounts of hydroxyapatite in polymethylmethacrylate samples promotes cell adhesion and proliferation.