RESUMEN
Three types of reaction of human blood leucocytes to herpes simplex virus 1 (HSV-1) were detected. The first reaction type, i.e. production of IFN-alpha, IFN-gamma, IL-1beta, IL-6 and TNFalpha but not of IL-2 or IL-4, denotes the primary body reaction to an infectious agent. The second reaction type is related with infection of activated HSV-1 leucocytes and is accompanied by an inhibited production of IFN-gamma, IL-6 and IL-8, which is targeted at suppressing the antivirus cell mechanisms. The third reaction type is associated with production of IFN-alpha, IFN-gamma, IL-beta, IL-6 and TNFalpha by blood leucocytes affected by HSV-1-infected leucocytes.
Asunto(s)
Citocinas/biosíntesis , Herpesvirus Humano 1/fisiología , Leucocitos/inmunología , Animales , Células Cultivadas , Chlorocebus aethiops , Técnicas de Cocultivo , Citocinas/análisis , Humanos , Interferones/análisis , Interferones/biosíntesis , Interleucinas/análisis , Interleucinas/biosíntesis , Leucocitos/virología , Activación de Linfocitos , Factores de Tiempo , Células VeroRESUMEN
The interferon status test characterizes the interferon (IFN) system function and the functional activity of IFN-producing cytokine cells. In contrast to the routine method, we used fetal calf (FCS) and autologous sera for evaluating the patients' leukocyte capacity to produce IFN-alpha and IFN-gamma. Blood samples from 30 women with genital infections caused by herpes simplex virus, cytomegalovirus, Chlamydia, and Ureaplasma were tested. Autologous sera of 40% patients inhibited and of 6.6% patients stimulated the production of IFN-gamma in comparison with FCS. All the data are statistically reliable. 20.3% autologous sera contained acid-labile IFN-alpha. These results can be used for more effective immunocorrective therapy.
Asunto(s)
Enfermedades de los Genitales Femeninos/inmunología , Interferón-alfa/inmunología , Interferón gamma/inmunología , Animales , Bovinos , Femenino , Enfermedades de los Genitales Femeninos/microbiología , Humanos , Leucocitos/inmunologíaRESUMEN
Cytokine production by donor blood leukocytes was investigated in vitro. LPS stimulation led to the production of IL-1 beta, IL-6, and TNF-alpha, if their spontaneous production was below the threshold sensitivity of the test method, and to a statistically negligible increase of production if spontaneous production was pronounced. The concentrations of cytokines produced depended on the nature of inductors and their doses. The inductor modulating effect can be used for stimulation and inhibition of cytokine production.