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1. Based on the hypothesis that 25-hydroxycholecalciferol (25-OH-D3) inclusion would optimise dietary mineral digestibility and ameliorate growth performance and bone mineralisation in available phosphorus (AvP) deficient-fed broilers, a trial was conducted to evaluate its effect on diets with different levels of AvP.2. Broilers aged 1-21 d were randomly assigned one of the eight treatments, consisting of four dietary levels of AvP (0.45%, 0.42%, 0.39%, and 0.36%) and with or without supplementation with 25-OH-D3 at 69 µg/kg of feed. All diets contained 100 µg/kg of vitamin D3 (cholecalciferol).3. The addition of 25-OH-D3 resulted in higher feed intake and body weight gain, and lower FCR (P < 0.05) compared to non-supplemented diets, whereas AvP levels had a quadratic effect only on feed intake. There were no interactions between treatment factors.4. Increasing AvP levels linearly reduced the ileal digestibility of Ca and P (P < 0.01) and supplementing 25-OH-D3 increased both Ca and P ileal digestibility (P < 0.05), without any interactions observed for ileal digestibility.5. There was an interaction, whereby 25-OH-D3 inclusion increased serum metabolites in broilers fed 0.36% to 0.42% AvP compared to the non-supplemented diets (P < 0.001), whereas, at 0.45% AvP, diets with or without 25-OH-D3 had similar results.6. The P content in bone linearly increased in line with AvP levels (P < 0.05) and supplementation of 25-OH-D3 increased ash bone content (P < 0.001).7. Broilers can benefit from 25-OH-D3 supplementation combined with cholecalciferol with regard to Ca and P utilisation and vitamin D status, allowing for a reduction of dietary AvP levels down to 0.36% without impairing growth performance or bone status.

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Objetivou-se avaliar o coeficiente de digestibilidade aparente (CDA) dos nutrientes, a palatabilidade das dietas e as características fecais de cães alimentados com uma dieta controle e uma dieta contendo 20% de gérmen desengordurado (GD), com e sem adição de complexo enzimático (amilase, xilanase, betaglucanase e mananase). Para o experimento de digestibidade e das características fecais, foram utilizados 12 cães adultos, distribuídos em delineamento em blocos ao acaso, em esquema fatorial 2 x 2 (dieta x enzima). O segundo experimento avaliou a palatabilidade, por meio da primeira escolha e da razão de ingestão (RI) da dieta DC vs. 20% de GD, utilizando-se 16 cães. O teste de palatabilidade contou com três dias consecutivos, totalizando 48 repetições. A dieta com inclusão de 20% de GD teve os menores valores de CDA da MS, da EB e da EM (P<0,05). A inclusão do complexo enzimático melhorou o CDA da MS, da EB e da EM (P<0,05). Não foram observadas diferenças nas características fecais (P>0,05). Em relação à palatabilidade, os cães preferiram a dieta 20% de GD, tanto na primeira escolha como na RI (P<0,05). A inclusão de enzimas às dietas melhora a digestibilidade dos nutrientes e da EM, sendo um aditivo com potencial uso na alimentação de cães.(AU)

The objective was to evaluate the apparent digestibility coefficient (ADC) of nutrients, diet palatability and fecal characteristics of dogs fed diets containing degreased germ (DG), and a control diet (DC) - both with and without the addition of enzyme complex (amylase, xylanase, betaglucanase and mananase). For the digestibility and fecal characteristics experiment 12 adult dogs were used, distributed in a randomized block design, in a 2 x 2 factorial scheme (diet x enzyme). The second experiment evaluated palatability using the first choice and ingestion ratio (IR) of DC diet vs. 20%gD, using 16 dogs. The palatability test had three consecutive days, totaling 48 repetitions. The diet with inclusion of 20% DG had the lowest ADC values of DM, GE and ME (P <0.05). Inclusion of the enzyme complex improved ADC of DM, GE and ME (P <0.05). No differences in fecal characteristics were observed (P >0.05). Regarding palatability, dogs preferred the 20% DG diet in both first choice and IR (P <0.05). Inclusion of enzymes in diets improves nutrient digestibility and ME, being an additive with potential use in dog food.(AU)

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The objective of this study was to evaluate the effect of storage time on albumen quality, incubation yield, and hatch window in Pekin ducks (Anas boschas). A total of 1302 eggs were randomly distributed to seven treatments according to the storage time with durations ranging from 1 to 14 days. Each treatment consisted of 186 eggs with a two-day storage interval between treatments. The pH and Haugh unit (HU) of the albumen, egg weight loss during incubation (WL), hatchability (HTCH), incubation duration (ID), hatch window, asymmetry (ASS), percentile kurtosis (PK), and embryonic mortality were analyzed. A linear effect was observed forthe WL, ID, and PK and a quadratic effect forpH, HU, and HTCH over time. No significant effect of storage time was observed on ASS. Post-pipping embryonic mortality was linearly affected by storage time. Prolonging the storage period above 10 days reduces albumen quality and hatchability, delays the hatch of Pekins, and results in a higher percentage of post-pipping mortality.

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The objective of this study was to evaluate the effect of storage time on albumen quality, incubation yield, and hatch window in Pekin ducks (Anas boschas). A total of 1302 eggs were randomly distributed to seven treatments according to the storage time with durations ranging from 1 to 14 days. Each treatment consisted of 186 eggs with a two-day storage interval between treatments. The pH and Haugh unit (HU) of the albumen, egg weight loss during incubation (WL), hatchability (HTCH), incubation duration (ID), hatch window, asymmetry (ASS), percentile kurtosis (PK), and embryonic mortality were analyzed. A linear effect was observed forthe WL, ID, and PK and a quadratic effect forpH, HU, and HTCH over time. No significant effect of storage time was observed on ASS. Post-pipping embryonic mortality was linearly affected by storage time. Prolonging the storage period above 10 days reduces albumen quality and hatchability, delays the hatch of Pekins, and results in a higher percentage of post-pipping mortality.(AU)

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Many requirements are necessary to meet the European Union rules to export poultry, including the amount of physiological water and water-protein ratio (WPR) in carcasses. Therefore, the aim of this study was to identify if strain, nutrition, and age affect the amount of collagen and fat and the WPR in cuts and verify whether the latter meets the international export standards. A total of 3,240 male chicks were housed in a completely randomized design in a 3 × 3 × 5 factorial arrangement, which included 3 nutritional densities (regular, medium, and high), 3 strains (021 Embrapa and 2 commercial strains identified as A and B), and 5 ages. Twelve broilers from each treatment (totaling 540 birds) were slaughtered at 28, 35, 42, 49, and 56 D of age to determine collagen and fat levels and WPR (through the calculation of moisture and protein percentage) in broiler breasts and legs using the near-infrared spectroscopy method. The use of feeds with different nutritional densities presented in this study has no effect on the WPR in the breast and legs of broilers slaughtered between 28 and 56 D of age. However, nutritional density influences liveweight and percentage of fat in the breast and legs. Collagen percentage in the legs decreases with increasing nutritional density. The 021 Embrapa strain cuts present a lower WPR than those of other commercial strains. However, the values found for all strains studied are within the limits of the Europe Union and Brazilian legislations. The liveweight, breast weight, leg weight, and leg fat increases linearly with age. Quite the opposite, water protein ratio, breast fat level, and breast collagen level decrease linearly with age. Leg WPR and leg collagen level are not affected by age. Despite the differences found for strains, nutritional densities and age readers should be aware that these factors may interact with each other depending on the response variable studied.

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Feed form is well recognized to improve broiler performance, specially by increasing feed intake (FI). However, when different diet energy levels are used, the results differ in the literature. Therefore, this experiment was conducted to evaluate the influence of feed form and dietary metabolizable energy (ME) levels on broiler performance, carcass yield and on the digestibility of DM, CP, starch and gross energy. In total, 1152 male Cobb 500 broilers were evaluated between 35 and 47 days. The birds were distributed according to a completely randomized design in a 2 × 4 factorial arrangement, consisting of two feed forms (mash or pellet) and four ME levels (12.73, 13.06, 13.40 or 13.73 MJ/kg), totaling eight treatments with eight replicates of 18 birds. Broilers fed the lowest ME level presented the lowest weight gain (WG) and worst feed per unit gain (P < 0.01). Metabolizable energy intake increased (P < 0.01) with progressive increments of ME, which, however, did not affect caloric conversion (CC, P > 0.05). Pelleted diets promoted higher FI, WG, ME intake (P < 0.01) and better feed per unit gain and CC (P < 0.05) compared with mash. In mash diets, increasing dietary ME levels promoted a linear increase in WG (P < 0.01) and reduced feed per unit gain (P ≤ 0.05), but did not affect FI (P > 0.05). In pelleted diets, on the other hand, increasing ME levels linearly reduced FI (P < 0.05) and feed per unit gain (P < 0.01). Broilers fed pelleted diets presented higher abdominal fat deposition than those fed mash (P < 0.05). Increasing ME levels reduced the coefficients of ileal apparent digestibility of DM (P < 0.01) and total starch (P < 0.05) but did not affect the digestibility of other evaluated nutrients. The digestibility of all nutrients was lower when pelleted diets were fed compared with mash. Increasing inert material inclusion in the diets at the expense of soybean oil to reduce dietary ME levels promoted higher pellet durability index values (P < 0.05) and the percentage of fines (P < 0.01). Overall, the results suggest that pelleted diets promote better broiler performance because they increase FI, since the digestibility of dietary fractions is reduced. Chickens consuming low-energy pelleted diets may increase FI to compensate for energy deficit. In contrast, broilers fed mash diets may have reached their maximum intake capacity and did not regulate FI by changing feed energy density. When feeding pelleted diets, dietary energy reduction should be considered to reduce feed costs and to improve the carcass quality of broilers.

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The dietary inclusion of feed additives to improve the carcass characteristics of the final product is of great importance for the pork production chain. The aim of our study was to evaluate the effects of the association of ractopamine (RAC) and conjugated linoleic acid (CLA) on the performance traits of finishing pigs during the last 26 days prior to slaughter. In total, 810 commercial hybrid barrows were used. Animals were distributed among treatments according to a randomised block design in a 3 × 3 factorial arrangement, with three RAC levels (0, 5 or 10 ppm) and three CLA levels (0, 0.3 or 0.6%). Pigs fed the diet with 5 ppm RAC had higher average daily feed intake (ADFI) (2.83 kg; P < 0.05) when compared with those fed 10 ppm RAC and the control diet (2.75 and 2.74 kg, respectively). Lower ADFI values (P < 0.01) were observed with the diets containing CLA compared with the control diet with no CLA (2.73 and 2.75 v. 2.85 kg/day, respectively). The average daily weight gain of pigs fed 5 and 10 ppm RAC was +148 and +173 g/dayhigher (P < 0.001), respectively, than those fed the control diet. Dietary RAC levels influenced (P < 0.001) feed conversion ratio (FCR), which was reduced as RAC levels increased, with the pigs fed 10, 5 and 0 ppm RAC presenting FCR values of 2.57, 2.71 and 3.05, respectively. FCR also improved (P < 0.05) with the inclusion of 0.6% CLA relative to the control diet (2.70 v. 2.84, respectively). There was a significant interaction between CLA × RAC levels (P < 0.01) for final BW, loin eye area (LEA) (P < 0.05) and backfat thickness (BT) (P < 0.05). The treatments containing 10 ppm RAC + 0.6% or 0.3% CLA increased LEA and reduced BT. In conclusion, the level of 10 ppm inclusion of RAC increased the overall performance parameters of pigs and therefore improved production efficiency. The combined use of RAC and CLA promoted a lower feed conversion ratio as well as better quantitative carcass traits, as demonstrated by the higher LEA and lower BT. The dietary inclusion of CLA at 0.3% improved feed efficiency, however, without affecting LEA or BT yields.

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The objective of the study was to evaluate the deposition of selenium in the breast muscle of broilers fed different selenium sources during different periods. Two experiments were carried out with broilers fed 45% sodium selenite (SS) or 2% hydroxy-selenomethionine (HSM) to determine the selenium deposition rate in the muscle tissue. In Experiment 1, 48 broilers were distributed in a completely randomized design with six treatments with eight replicates each, consisting of diets containing 0 Se (0), 200 or 400 µgSS/kg (200 SS, 400 SS), or 100, 200, or 300 µgHSM/kg (100 HSM, 200 HSM, 300 HSM) for 10 days. The highest Se breast content was obtained with 300 HSM diet, followed by 200 SS diet compared to the other treatments. Experiment 2 had a completely randomized design following a plot scheme subdivided in time, and consisted of a basal diet supplemented with 300 µg SS/kg (300 SS), basal diet with 200 µg HSM/kg (200 HSM) or basal diet supplemented with 100 µgSS/kg and 100 µgHSM/kg (100 SS+100 HSM). Dietary Se sources did not influence Se breast content measured on days 3 and 6. However, on days 9 and 12, higher Se breast contents were measured in broilers fed 200 HSM and 100 SS+100 HSM diets compared with those fed 300 SS diet. In conclusion, the supplementation of broiler diets with HSM at 2% for a short period is more efficient to increase selenium deposition in the breast muscle than sodium selenite.

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The objective of the study was to evaluate the deposition of selenium in the breast muscle of broilers fed different selenium sources during different periods. Two experiments were carried out with broilers fed 45% sodium selenite (SS) or 2% hydroxy-selenomethionine (HSM) to determine the selenium deposition rate in the muscle tissue. In Experiment 1, 48 broilers were distributed in a completely randomized design with six treatments with eight replicates each, consisting of diets containing 0 Se (0), 200 or 400 µgSS/kg (200 SS, 400 SS), or 100, 200, or 300 µgHSM/kg (100 HSM, 200 HSM, 300 HSM) for 10 days. The highest Se breast content was obtained with 300 HSM diet, followed by 200 SS diet compared to the other treatments. Experiment 2 had a completely randomized design following a plot scheme subdivided in time, and consisted of a basal diet supplemented with 300 µg SS/kg (300 SS), basal diet with 200 µg HSM/kg (200 HSM) or basal diet supplemented with 100 µgSS/kg and 100 µgHSM/kg (100 SS+100 HSM). Dietary Se sources did not influence Se breast content measured on days 3 and 6. However, on days 9 and 12, higher Se breast contents were measured in broilers fed 200 HSM and 100 SS+100 HSM diets compared with those fed 300 SS diet. In conclusion, the supplementation of broiler diets with HSM at 2% for a short period is more efficient to increase selenium deposition in the breast muscle than sodium selenite.(AU)

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This study evaluated the effect of birth weight and weight gain during the suckling phase on the piglet daily weight gain during the nursery phase (21-61 days of age). Piglets (n= 534) derived from 55 first-parity sows were weighed at birth, weaning, and end of the nursery phase. The data were analyzed according to a completely randomized experimental design, with three classes of birth weight (BW) and two classes of weight gain during the suckling phase (SDWG). High BW and low SDWG piglets were 0.2kg lighter at weaning than low BW and high SDWG animals (P< 0.05). However, at the end of the nursery phase, this situation was inverted, with high BW and low SDWG piglets 2.20kg heavier (P< 0.05). Low BW and high SDWG piglets were heavier at weaning and at the end of the nursery phase than low BW and low SDWG piglets (P< 0.05). At the same time, intermediate and high BW and low and high SDWG piglets presented body weight differences at weaning (P< 0.05), but not at the end of the nursery phase (P< 0.05). Piglets with 1.25 to 2.30kg BW and low weight gain during suckling presented a partial compensatory growth during the nursery phase.(AU)

Objetivou-se avaliar o efeito do peso dos leitões ao nascimento e do seu ganho de peso na maternidade sobre seu desempenho na fase de creche (21-61 dias de idade). Quinhentos e trinta e quatro leitões, provenientes de 55 primíparas, foram pesados ao nascimento, no desmame e na saída da creche. Os animais foram distribuídos em um delineamento inteiramente ao acaso, em três classes de peso ao nascimento (PN) e duas classes de ganho de peso diário (GPD) na maternidade. Os animais com alto PN e baixo GPD na maternidade desmamaram 0,2kg mais leves que os animais com baixo PN e alto GPD na maternidade (P<0,05). Porém, os animais com alto PN e baixo GPD na maternidade saíram da creche 2,10kg mais pesados (P<0,05). A diferença aos 21 dias entre os dois tratamentos (alto e baixo GPD) com animais de baixo PN foi de 1,18kg e passou para 2,20kg ao final da creche (P<0,05). No caso dos animais com médio e alto PN, a diferença ao desmame era de 1,14 e 1,26kg e passou para 0,90 e 0,70kg na saída da creche (P<0,05), respectivamente. Leitões com peso ao nascimento entre 1,25 e 2,30kg, com baixo ganho de peso diário na maternidade, apresentaram efeito compensatório parcial no ganho de peso diário durante a creche.(AU)

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This study evaluated the effect of birth weight and weight gain during the suckling phase on the piglet daily weight gain during the nursery phase (21-61 days of age). Piglets (n= 534) derived from 55 first-parity sows were weighed at birth, weaning, and end of the nursery phase. The data were analyzed according to a completely randomized experimental design, with three classes of birth weight (BW) and two classes of weight gain during the suckling phase (SDWG). High BW and low SDWG piglets were 0.2kg lighter at weaning than low BW and high SDWG animals (P< 0.05). However, at the end of the nursery phase, this situation was inverted, with high BW and low SDWG piglets 2.20kg heavier (P< 0.05). Low BW and high SDWG piglets were heavier at weaning and at the end of the nursery phase than low BW and low SDWG piglets (P< 0.05). At the same time, intermediate and high BW and low and high SDWG piglets presented body weight differences at weaning (P< 0.05), but not at the end of the nursery phase (P< 0.05). Piglets with 1.25 to 2.30kg BW and low weight gain during suckling presented a partial compensatory growth during the nursery phase.(AU)

Objetivou-se avaliar o efeito do peso dos leitões ao nascimento e do seu ganho de peso na maternidade sobre seu desempenho na fase de creche (21-61 dias de idade). Quinhentos e trinta e quatro leitões, provenientes de 55 primíparas, foram pesados ao nascimento, no desmame e na saída da creche. Os animais foram distribuídos em um delineamento inteiramente ao acaso, em três classes de peso ao nascimento (PN) e duas classes de ganho de peso diário (GPD) na maternidade. Os animais com alto PN e baixo GPD na maternidade desmamaram 0,2kg mais leves que os animais com baixo PN e alto GPD na maternidade (P<0,05). Porém, os animais com alto PN e baixo GPD na maternidade saíram da creche 2,10kg mais pesados (P<0,05). A diferença aos 21 dias entre os dois tratamentos (alto e baixo GPD) com animais de baixo PN foi de 1,18kg e passou para 2,20kg ao final da creche (P<0,05). No caso dos animais com médio e alto PN, a diferença ao desmame era de 1,14 e 1,26kg e passou para 0,90 e 0,70kg na saída da creche (P<0,05), respectivamente. Leitões com peso ao nascimento entre 1,25 e 2,30kg, com baixo ganho de peso diário na maternidade, apresentaram efeito compensatório parcial no ganho de peso diário durante a creche.(AU)

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Small eggs have lesser amounts of nutrients to be used by the embryo, and the yolk glycerol is the main substrate for glycogen production, which is the main energy source in the last days of incubation. Thus, the present study aimed to assess the effect of a glycerol injection in light weight eggs at 2 different days of incubation. To this end, 336 light eggs (55.6 to 58.6 g) from 32-wk-old broiler breeders were incubated. The eggs were divided into 3 treatment groups: 1 group inoculated with saline solution on the 17th d of embryonic development (E17) (control group), the second group injected with a 6 mg glycerol/mL solution at E17, and the third group injected with 6 mg glycerol/mL on the 18th d of incubation (E18). Incubation parameters, liver and muscle glycogen, and broilers performance at 7 d of age were evaluated. Glycerol administration in ovo did not influence hatchability, period of embryonic death or early hatching. Chicks exposed to glycerol in ovo feeding (IOF) used more yolk than birds inoculated with saline solution. Glycerol inoculation at E18 enhanced liver glycogen deposition (P = 0.001) and also improved broilers performance at 7 d, although this improvement in performance and glycogen reserves was not observed when eggs were inoculated at 17 d of incubation. Birds receiving glycerol IOF at E18 showed higher feed intake and body weight gain when compared to the control group and the group inoculated at E17. It was found that glycerol inoculation in light eggs at the 18th d of incubation contributed to raise liver glycerol levels and also to improve broilers performance at 7 d.

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Objetivou-se avaliar a forma de inclusão da zeólita e de níveis de consumo sobre a digestibilidade da dieta e as características fecais de cães. O experimento 1 avaliou o efeito do nível de consumo (normal e 50% a mais do que as necessidades) e da inclusão de zeólita (0 e 15g/kg) sobre a digestibilidade e as características fecais de 12 cães. No experimento 2, foi avaliada a inclusão de 5, 15 e 25g/kg de zeólita adicionada na massa ou por cobertura, em seis cães, sobre a digestibilidade da dieta e as características fecais. O experimento 3 avaliou o odor fecal de nove cães consumindo dietas contendo 0 e 15g/kg de zeólita incluída na massa ou por cobertura. A quantidade consumida e a inclusão de zeólita não afetaram a digestibilidade das dietas. O escore e a matéria seca fecal aumentaram com a inclusão de zeólita. A inclusão de zeólita na massa propiciou aumento na digestibilidade das dietas, independentemente do nível de inclusão. A inclusão de 15g/kg de zeólita, adicionada na massa e por cobertura reduziu o odor fecal dos cães. A inclusão de zeólita na massa aumenta a digestibilidade da dieta, a consistência fecal e reduz o odor fecal de cães.(AU)

This study aimed to evaluate the form of inclusion of zeolite and consumption levels on diet digestibility and fecal characteristics of dogs. The first experiment examined the effect of consumption level (normal and 50% higher than the requirements) and zeolite level (0 and 15g/kg) on diet digestibility and fecal characteristics of 12 dogs. In experiment 2 the inclusion of 5, 15 and 25g/kg of added zeolite in dough or coating in six dogs on diet digestibility and fecal characteristics was evaluated. The third experiment assessed the fecal odor of nine dogs fed diets containing 0 and 15g/kg of zeolite included in the dough or coating. The amount consumed, as well as the inclusion of zeolite, did not affect diet digestibility. Fecal score and dry matter increased with the inclusion of zeolite. The inclusion of zeolite in the dough provided an increase in diet digestibility, regardless of the level of inclusion. The inclusion of 15g/kg zeolite added to the dough and by coating reduced fecal odor of dogs. The inclusion of zeolite in dough increases diet digestibility, fecal consistency, and reduces the fecal odor of dogs.(AU)

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Objetivou-se avaliar a forma de inclusão da zeólita e de níveis de consumo sobre a digestibilidade da dieta e as características fecais de cães. O experimento 1 avaliou o efeito do nível de consumo (normal e 50% a mais do que as necessidades) e da inclusão de zeólita (0 e 15g/kg) sobre a digestibilidade e as características fecais de 12 cães. No experimento 2, foi avaliada a inclusão de 5, 15 e 25g/kg de zeólita adicionada na massa ou por cobertura, em seis cães, sobre a digestibilidade da dieta e as características fecais. O experimento 3 avaliou o odor fecal de nove cães consumindo dietas contendo 0 e 15g/kg de zeólita incluída na massa ou por cobertura. A quantidade consumida e a inclusão de zeólita não afetaram a digestibilidade das dietas. O escore e a matéria seca fecal aumentaram com a inclusão de zeólita. A inclusão de zeólita na massa propiciou aumento na digestibilidade das dietas, independentemente do nível de inclusão. A inclusão de 15g/kg de zeólita, adicionada na massa e por cobertura reduziu o odor fecal dos cães. A inclusão de zeólita na massa aumenta a digestibilidade da dieta, a consistência fecal e reduz o odor fecal de cães.(AU)

This study aimed to evaluate the form of inclusion of zeolite and consumption levels on diet digestibility and fecal characteristics of dogs. The first experiment examined the effect of consumption level (normal and 50% higher than the requirements) and zeolite level (0 and 15g/kg) on diet digestibility and fecal characteristics of 12 dogs. In experiment 2 the inclusion of 5, 15 and 25g/kg of added zeolite in dough or coating in six dogs on diet digestibility and fecal characteristics was evaluated. The third experiment assessed the fecal odor of nine dogs fed diets containing 0 and 15g/kg of zeolite included in the dough or coating. The amount consumed, as well as the inclusion of zeolite, did not affect diet digestibility. Fecal score and dry matter increased with the inclusion of zeolite. The inclusion of zeolite in the dough provided an increase in diet digestibility, regardless of the level of inclusion. The inclusion of 15g/kg zeolite added to the dough and by coating reduced fecal odor of dogs. The inclusion of zeolite in dough increases diet digestibility, fecal consistency, and reduces the fecal odor of dogs.(AU)

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An experiment was carried out to evaluate the digestibility and metabolizability of corn and a corn/soy-based diet with the inclusion or not of xylanase and/or phytase in broilers. In the trial, 1,120 broiler chicks were distributed according to a completely randomized experimental design, consisting of 16 treatments, with 10 replicates of 7 birds each. Treatments were evaluated following a factorial arrangement (4 × 2 × 2), with 4 xylanase levels (zero, 50, 100, or 150 fungal ß-xylanase units/kg; FXU), 2 phytase levels (zero or 1,000 phytase units/kg; FTU), and 2 diets (corn/soy or pure corn). The same basal diets were fed from one to 14 d post hatch for all birds, after which the experimental diets were provided until d 25. All birds were euthanized on d 25 for collection of ileal contents. Samples of feed, excreta, and ileal digesta were analyzed for determination of apparent ileal digestibility. The effect of xylanase on the coefficient of apparent dry matter metabolizability and apparent metabolizable energy was increased by the presence of phytase in the complete diet but not in the diet based on pure corn resulting in a diet*phytase*xylanase interaction (P < 0.01; P < 0.001, respectivaly). Equivalent effects were observed for the apparent coefficient of ileal protein digestibility in which xylanase effects were potentiated by the presence of phytase only in the complete diet, resulting in a significant 3-way interaction. In corn there was a limitation in improving digestibility when we added increasing levels of xylanase with phytase. Otherwise in the corn/soy-based diets, the enzymes were potencialized when they were added together. The fact that the effect of xylanase was enhanced by the presence of phytase in complete diets but not in pure corn may be associated with differences in substrate (arabinoxylan and/or phytate) concentration and presentation, diet nutrient balance, or other factors. It can be concluded that the interactive effects of xylanase and phytase can be substantial but may depend on the characteristics of the diet fed.

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This study investigated the effect of broiler breeder age on the morphological development of the small intestine broiler embryos (villus height, crypt depth, microvillus height, and villus density) at 20 day of incubation. Eggs obtained from 30- and 60-wk-old broiler breeders were used. The results showed that embryos from older broiler breeders presented longer villi in the duodenum, jejunum, and ileum compared with younger broiler breeders. In addition, embryos from older broiler breeders presented deeper crypts in the jejunum and ileum, longer microvilli in jejunal enterocytes, and lower villus density (microvillus number/mm2) in the duodenum and ileum than younger breeders. These results suggest that breeder age influences the gut mucosa development of broiler embryos. Embryos from older broiler breeder showed greater development of the small intestine mucosa than those from younger broiler breeder.

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This study investigated the effect of broiler breeder age on the morphological development of the small intestine broiler embryos (villus height, crypt depth, microvillus height, and villus density) at 20 day of incubation. Eggs obtained from 30- and 60-wk-old broiler breeders were used. The results showed that embryos from older broiler breeders presented longer villi in the duodenum, jejunum, and ileum compared with younger broiler breeders. In addition, embryos from older broiler breeders presented deeper crypts in the jejunum and ileum, longer microvilli in jejunal enterocytes, and lower villus density (microvillus number/mm2) in the duodenum and ileum than younger breeders. These results suggest that breeder age influences the gut mucosa development of broiler embryos. Embryos from older broiler breeder showed greater development of the small intestine mucosa than those from younger broiler breeder.(AU)

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An experiment was conducted to test the hypothesis that a coarse limestone diet improves productivity, reproductive performance and the calcium utilization of molted broiler breeders. In total, 640 broiler breeder females, 73-week-old and sixty-four 27-week-old cockerels, Cobb 500, were evaluated during 10 weeks, according to a randomized block design composed of 4 treatments with 8 replicates each. Treatments consisted of diets with the inclusion of 100% fine limestone-fine PS (0.2 mm GMD-geometric mean diameter); PS1: 30% fine limestone+70% limestone with 1.0 mm GMD; PS2: 30% fine limestone+70% limestone with 2.0 mm GMD; and PS3: 30% fine limestone+70% limestone with 3.0 mm GMD. Calcium retention in the gizzard of the breeders, bone characteristics, and breeder performance, egg characteristics, eggshell quality, incubation performance, chick quality and yield, chick pre-starter live performance, and chick bone characteristics were determined. There was no significant difference (P>0.05) in the rate of lay, percentage of non-settable eggs, egg weight, egg shape index, egg specific gravity, eggshell weight, thickness, and percentage hatchability and egg weight loss of broiler breeders fed with diets with different limestone particle sizes. The chick quality and yield, chick pre-starter live performance, and chick bone characteristics were not affected (P>0.05) by any of the limestone particle sizes. It was concluded that live and reproductive performance parameters of broiler breeders post molting is not affected by limestone particle size in the feed.

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A total of 50 mixed parity sows of a high-prolificacy genetic line were used to evaluate the impact of feed restriction during lactation on their production and reproductive performance and their performance in the subsequent lactation. From day 7 of lactation, sows were distributed according to a completely randomized experimental design into two treatments. In treatment 1, sows were fed 8.0 kg feed/day (control) and in treatment 2, sows were fed 4.0 kg/day. The same suckling pressure was maintained until weaning on day 28 of lactation. Average minimum and maximum temperatures measured during the experimental period were 32.1°C and 16.5°C, respectively. Control sows presented significantly higher feed intake (P0.10) in weaning-to-estrus interval and averaged 4.3 days. No effect of the treatment (P>0.10) was observed on any of the studied performance traits in the subsequent lactation, except for litter size at birth that tended (15.2 v. 14.1; P<0.10) to be lower for the restricted sows. In conclusion, the present study demonstrated that feed restriction during lactation leads to intense catabolism of the body tissues of sows, negatively affecting their milk production, and the litter weight gain and possibly number of piglets born in the next litter. On the other hand, restricted-fed sows are more efficient, producing more milk per amount of feed intake.

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The aim of this study was to evaluate the possible effects of glutamine and broiler breeder age on the development of intestinal mucosa in broiler chicks during first week of age. For this purpose, 32 one-day-old broiler chicks were distributed according to a completely randomized experimental design in a 2 x 2 factorial arrangement. Treatments consisted of two broiler breeder ages (30 and 60 weeks) and two dietary glutamine supplementation levels (0% and 1%). The morphological development of intestinal mucosa, particularly villus height and crypt depth, was evaluated. The results showed that the supplementation with 1% of glutamine influenced the development of villus height in the duodenum (p=0.009), jejunum (p = 0.006), and ileum (p = 0.001), as well as crypt depth in the jejunum (p = 0.037) of 7-day-old broilers. These results suggest that the presence of glutamine influenced the development of intestinal mucosa during the first week of age, when these tissues are highly influenced by dietary components, especially by trophic agents. The results show that broiler breeder age (30 or 60 weeks) did not influence the evaluated parameters.

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