RESUMEN
Ewing sarcoma (ES) is one of the most aggressive types of pediatric tumors. The lack of tools for the identification of ES has largely hindered clinical diagnosis and the improvement of treatment. To address this challenge, we synthesized a near-infrared (NIR) fluorescent probe (CS2-N-E9R) that targets the ES-specific fusion protein EWS-FLI1 (E/F). This probe exhibited specific and high binding affinity to E/F. Further studies in animal models showed that CS2-N-E9R can be used to identify the boundaries of ES and lymph node metastases under a complex biological environment. These results demonstrate that CS2-N-E9R is a promising probe for early diagnosis and surgical guidance of ES through molecularly targeted NIR imaging.
Asunto(s)
Sarcoma de Ewing , Animales , Línea Celular Tumoral , Regulación Neoplásica de la Expresión Génica , Humanos , Metástasis Linfática , Proteínas de Fusión Oncogénica/genética , Proteínas de Fusión Oncogénica/metabolismo , Proteína Proto-Oncogénica c-fli-1 , Proteína EWS de Unión a ARN/genética , Sarcoma de Ewing/diagnóstico por imagen , Sarcoma de Ewing/tratamiento farmacológicoRESUMEN
A pH-sensitive near-infrared fluorescent probe with alkaline pKa, AlkaP-1, was developed by incorporating a benzoyl hydrazine group into a cyanine dye. The significant fluorescence changes in the alkaline regions enable the probe to monitor the alkalization process from acute wounds to chronic wounds in diabetic mice.
Asunto(s)
Colorantes Fluorescentes/química , Heridas y Lesiones/diagnóstico , Animales , Carbocianinas/química , Línea Celular Tumoral , Enfermedad Crónica , Diabetes Mellitus Experimental/metabolismo , Colorantes Fluorescentes/síntesis química , Humanos , Hidrazinas/química , Concentración de Iones de Hidrógeno , Rayos Infrarrojos , Ratones Transgénicos , Imagen Óptica , Heridas y Lesiones/metabolismoRESUMEN
Carbon monoxide (CO) is recognized as a biologically essential gaseous neurotransmitter that modulates many physiological processes in living subjects. Currently reported fluorescent probes for CO imaging in cells basically utilize palladium related chemistry which requires complicated synthetic work. Herein we provide a new strategy to construct a fluorescent nanoprobe, NanoCO-1, based on the Forster resonance energy transfer (FRET) mechanism by entrapping the existing dirhodium complex as the energy acceptor and the CO recognition part, and a commonly used nitrobenzoxadiazole (NBD) dye as energy donor into a micelle formed by self-assembly. The exchange of ligands in the dirhodium complex by CO in the nanoprobe disrupts the FRET and leads to the turn-on of fluorescence. The merits of NanoCO-1 including good biocompatibility, selectivity, photostability, and low cytotoxity, render this nanoprobe ability to track CO in living cells, zebrafish embryo, and larvae. Our straightforward approach can be extended to establish the CO fluorescent probes based on adsorption of CO on a variety of metal derivatives.