RESUMEN
Many characteristics of the South American teleost fish Cichlasoma dimerus (body size, easy breeding, undemanding maintenance) make it amenable to laboratory studies. In the last years, many of the fundamental aspects of its reproductive and developmental biology have been addressed in our laboratory. Rather recently, the immunohistochemical localization of pituitary hormones involved in reproduction and in background color adaptation has been described in both adult and developing individuals, and the role of FSH in ovarian differentiation has been established. These findings have been correlated with mapping of some of their brain-derived controlling hormones. The latter include brain-derived gonadotropins which were shown to be active in vitro in the control of pituitary hormone secretions. The emerging picture shows C. dimerus as an interesting species in which many of their basic features have already been investigated and which conform a solid platform for comparative studies correlating neurohormones, pituitary hormones and behavior, from the molecular to the organismic level.
Asunto(s)
Cíclidos/embriología , Cíclidos/fisiología , Gonadotropinas/metabolismo , Ovario/embriología , Hormonas Hipofisarias/metabolismo , Testículo/embriología , Animales , Encéfalo/metabolismo , Femenino , Masculino , Ovario/citología , Hipófisis/anatomía & histología , Hipófisis/metabolismo , Reproducción , Testículo/citologíaRESUMEN
Many characteristics of the South American teleost fish Cichlasoma dimerus (body size, easy breeding, undemanding maintenance) make it amenable to laboratory studies. In the last years, many of the fundamental aspects of its reproductive and developmental biology have been addressed in our laboratory. Rather recently, the immunohistochemical localization of pituitary hormones involved in reproduction and in background color adaptation has been described in both adult and developing individuals, and the role of FSH in ovarian differentiation has been established. These findings have been correlated with mapping of some of their brain-derived controlling hormones. The latter include brain-derived gonadotropins which were shown to be active in vitro in the control of pituitary hormone secretions. The emerging picture shows C. dimerus as an interesting species in which many of their basic features have already been investigated and which conform a solid platform for comparative studies correlating neurohormones, pituitary hormones and behavior, from the molecular to the organismic level.(AU)
Asunto(s)
Masculino , Animales , Femenino , Encéfalo/metabolismo , Cíclidos/embriología , Cíclidos/fisiología , Gonadotropinas/metabolismo , Ovario/embriología , Hipófisis/metabolismo , Testículo/citología , Testículo/embriología , Hormonas Hipofisarias/metabolismo , ReproducciónRESUMEN
Many characteristics of the South American teleost fish Cichlasoma dimerus (body size, easy breeding, undemanding maintenance) make it amenable to laboratory studies. In the last years, many of the fundamental aspects of its reproductive and developmental biology have been addressed in our laboratory. Rather recently, the immunohistochemical localization of pituitary hormones involved in reproduction and in background color adaptation has been described in both adult and developing individuals, and the role of FSH in ovarian differentiation has been established. These findings have been correlated with mapping of some of their brain-derived controlling hormones. The latter include brain-derived gonadotropins which were shown to be active in vitro in the control of pituitary hormone secretions. The emerging picture shows C. dimerus as an interesting species in which many of their basic features have already been investigated and which conform a solid platform for comparative studies correlating neurohormones, pituitary hormones and behavior, from the molecular to the organismic level.
Asunto(s)
Masculino , Animales , Femenino , Cíclidos/embriología , Cíclidos/fisiología , Encéfalo/metabolismo , Gonadotropinas/metabolismo , Hipófisis/metabolismo , Hormonas Hipofisarias/metabolismo , Ovario/embriología , Testículo/citología , Testículo/embriología , ReproducciónRESUMEN
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play key roles in vertebrate gametogenesis and steroidogenesis. They are mainly synthesized in the pituitary gland. While investigating the ontogeny of FSH and LH cells in the cichlid fish Cichlasoma dimerus by immunohistochemistry (IHC), we unexpectedly found immunoreactive neurons in the preoptic area, sending their projections through different brain areas and neurohypophysis. Our previous work using Western blot and IHC techniques applied to the adult brain confirmed these findings. To further demonstrate the extrapituitary expression of these hormones, we performed RT-PCR detecting sequences coding for beta-FSH and beta-LH subunits in the C. dimerus pituitary and brain (preoptic-hypothalamic area). The expression of these transcripts in both organs was consistent with their peptide expression showing a high sequence homology when compared with other phylogenetically related fish. An individual pituitary in vitro culture system was utilized to study the possible modulatory effect of brain-derived gonadotropins on pituitary hormone secretion. Pituitary explants were cultured with different concentrations of LH or FSH, and the culture media were analyzed by Western blot. Exogenous LH produced a dose-dependent increase in pituitary beta-LH, beta-FSH and somatolactin (SL) releases. No effect was observed on growth hormone (GH). The effect on prolactin (PRL) was not consistent among treatments. Exogenous FSH produced an inhibition in beta-LH release, dose-dependent increases in beta-FSH and SL releases, and no effect on PRL and GH releases. These findings support the concept of regulation of pituitary trophic hormones by brain-derived gonadotropins.
Asunto(s)
Hormona Folículo Estimulante de Subunidad beta/análisis , Hormona Folículo Estimulante de Subunidad beta/genética , Hormona Luteinizante de Subunidad beta/análisis , Hormona Luteinizante de Subunidad beta/genética , Hipófisis/metabolismo , Área Preóptica/química , ARN Mensajero/análisis , Secuencia de Aminoácidos , Animales , Cíclidos , Relación Dosis-Respuesta a Droga , Femenino , Proteínas de Peces/metabolismo , Hormona Folículo Estimulante/farmacología , Hormona Folículo Estimulante de Subunidad beta/metabolismo , Expresión Génica , Glicoproteínas/metabolismo , Hormona del Crecimiento/metabolismo , Inmunohistoquímica , Hormona Luteinizante/farmacología , Hormona Luteinizante de Subunidad beta/metabolismo , Masculino , Datos de Secuencia Molecular , Técnicas de Cultivo de Órganos , Hormonas Hipofisarias/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de AminoácidoRESUMEN
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) expressing cells were detected in pituitary, brain and ovary of the Perciform cichlid fish Cichlasoma dimerus. This detection was carried out by immunohistochemistry (IHC) and Western blot techniques using antisera of the Cyprinodontiform Fundulus heteroclitus raised against the conservative region of the teleost betaFSH and the betaLH subunits. The estimated molecular weights were 24 kDa for LH and 19 and 15 kDa for FSH. In the adult pituitary, both cell types were distributed along mid and ventral zones of the proximal pars distalis (PPD, mid-immunoreactive cells), and along the ventral and dorsal external border of the pars intermedia (PI, high-immunoreactive cells). Double IHC showed that FSH and LH are mainly expressed in different pituitary cells. FSH cells were detected in the pituitary around day 21 after hatching (ah) (prior to sex differentiation), while LH cells were detected by day 60 ah (during the sexual differentiation period). A correlation between gonadal sex differentiation and FSH was demonstrated in a 15 days organ culture system. FSH and LH neurons were localized in the nucleus lateralis tuberis and their fibers project through the ventral hypothalamus, preoptic area and neurohypophysis. FSH neurons differentiated on day 21 ah, while LH neurons appeared on day 15 ah. In the ovary, the immunoreactivity for both FSH and LH was restricted to the cytoplasm of previtellogenic and early vitellogenic oocytes.
Asunto(s)
Cíclidos/embriología , Hormona Folículo Estimulante/metabolismo , Hormona Luteinizante/metabolismo , Ovario/embriología , Hipófisis/embriología , Diferenciación Sexual , Factores de Edad , Animales , Encéfalo/citología , Encéfalo/embriología , Encéfalo/metabolismo , Femenino , Hormona Folículo Estimulante/farmacología , Hormona Liberadora de Gonadotropina/metabolismo , Inmunohistoquímica , Masculino , Fibras Nerviosas/metabolismo , Técnicas de Cultivo de Órganos , Ovario/citología , Ovario/metabolismo , Hipófisis/citología , Hipófisis/metabolismo , Testículo/citología , Testículo/embriología , Testículo/metabolismo , Vitelogénesis/efectos de los fármacos , Vitelogénesis/fisiologíaRESUMEN
Using immunocytochemistry we have described the distribution and ontogeny of three distinct gonadotropin-releasing hormone (GnRH) neural systems, emphasizing the analysis during the period of sex differentiation in the South American cichlid fish Cichlasoma dimerus. In the forebrain a group of neurones immunoreactive to salmon GnRH that formed clusters in the nucleus olfacto retinalis (NOR), was located at the junction of the olfactory bulb and the telencephalon. These neurones differentiated 3 days after fertilization from the olfactory placodes. GnRH immunoreactive neurones along the olfactory nerves through the rostrobasal olfactory bulb were observed on day 4 and at the NOR on day 10. A group of neurones immunoreactive to chicken GnRH II was seen in the dorsal midbrain tegmentum. They originate from the ventricular ependyma between days 5 and 6. These neurones remained close to blood vessels throughout development. Between days 22 and 30 a group of neurones immunoreactive to seabream GnRH was detected in the anterior basal preoptic area. GnRH innervation of the pituitary was detected after the differentiation of preoptic neurones and in coincidence with gonadal differentiation. We hypothesize that the GnRH neural systems have three distinct embryonic origins. Furthermore, we show that the NOR and the midbrain GnRH neurones might have functions other than gonadal development, whereas the preoptic GnRH neurones in C. dimerus might be associated with gonadal sex differentiation.