RESUMEN
PURPOSE: The importance of keeping the ileocecal valve in the intestinal ressections has been reported by several authors. When preserved, the ileocecal valve was related to a longer survival and prevention of the short bowel syndrome, due to its ability to block the colonic content reflux into the ileum and to avoid the rapid empting of the ileal content into the cecum. It was assessed a tecnique of ileocecal valve reconstitution, based on vesicoureteral anti-reflux tecniques. METHODS: Fourteen beagles were operated. Seven underwent ileocecal valve reconstitution following the tecnique proposed and in the other seven a simple end-to-end anastomosis was performed. To assess the new valve, it was done the clinical follow up, the microbiologic analysis and the manometric study. RESULTS: Clinically, during 45 days of follow up, there was no difference between the dogs with and without ileocecal reconstitution. In the aerobic bacteria analysis, the predominant bacterium was Escherichia coli. Quantitatively, the cultures grew in an irregular way, so that it was not able to compare the bacterial growth between the groups with or without ileocecal valve. The new valve had a colo-ileal reflux pressure similar to that of the physiological valve (P > 0.05). However, when compared to the non valve group, the reflux pressures of the physiological valve and new valve were significantly higher, with P < 0.05 and P < 0.001, respectively. CONCLUSION: In this study, the reconstituted ileocecal valve served as a barrier to the colo-ileal reflux just as the physiological valve does.
Asunto(s)
Válvula Ileocecal/cirugía , Animales , Procedimientos Quirúrgicos del Sistema Digestivo/métodos , Perros , MasculinoRESUMEN
OBJETIVO: Avaliar uma técnica de reconstituição da válvula ileocecal com base em técnicas anti-refluxo vesicoureteral. MÉTODOS: Quatorze beagles foram operados. Em sete foi reconstituída a válvula ileocecal com a técnica proposta e nos outros sete realizou-se apenas a anastomose ileocólica término-terminal. Para avaliar a neoválvula realizou-se o acompanhamento clínico dos cães, a análise microbiológica e o estudo manométrico. RESULTADOS: Clinicamente, durante os 45 dias de pós-operatório, não houve diferença entre os cães com e sem reconstituição da válvula ileocecal. Na análise de bactérias aeróbias, a bactéria predominante foi a Escherichia coli. Quantitativamente, as culturas cresceram de modo irregular, não permitindo a comparação do crescimento bacteriano entre os grupos com e sem válvula ileocecal. A neoválvula apresentou uma pressão de refluxo ileocólica semelhante a da válvula ileocecal fisiológica (P>0,05). Em relação ao grupo sem válvula, as pressões de refluxo da válvula fisiológica e da neoválvula foram significantemente maiores, sendo P<0,05 e P<0,001, respectivamente. CONCLUSAO: A neoválvula ileocecal serviu de obstáculo ao refluxo colo-ileal de maneira semelhante à válvula ileocecal fisiológica.
Asunto(s)
Animales , Masculino , Perros , Válvula Ileocecal/cirugía , Procedimientos Quirúrgicos del Sistema Digestivo/métodosRESUMEN
OBJETIVO: Avaliar uma técnica de reconstituição da válvula ileocecal com base em técnicas anti-refluxo vesicoureteral. MÉTODOS: Quatorze beagles foram operados. Em sete foi reconstituída a válvula ileocecal com a técnica proposta e nos outros sete realizou-se apenas a anastomose ileocólica término-terminal. Para avaliar a neoválvula realizou-se o acompanhamento clínico dos cães, a análise microbiológica e o estudo manométrico. RESULTADOS: Clinicamente, durante os 45 dias de pós-operatório, não houve diferença entre os cães com e sem reconstituição da válvula ileocecal. Na análise de bactérias aeróbias, a bactéria predominante foi a Escherichia coli. Quantitativamente, as culturas cresceram de modo irregular, não permitindo a comparação do crescimento bacteriano entre os grupos com e sem válvula ileocecal. A neoválvula apresentou uma pressão de refluxo ileocólica semelhante a da válvula ileocecal fisiológica (P>0,05). Em relação ao grupo sem válvula, as pressões de refluxo da válvula fisiológica e da neoválvula foram significantemente maiores, sendo P<0,05 e P<0,001, respectivamente. CONCLUSAO: A neoválvula ileocecal serviu de obstáculo ao refluxo colo-ileal de maneira semelhante à válvula ileocecal fisiológica. (AU)
Asunto(s)
Animales , Masculino , Perros , Válvula Ileocecal/cirugía , Procedimientos Quirúrgicos del Sistema Digestivo/métodosRESUMEN
We report the first case of digestive tract pathology (megaesophagus) determined by Trypanosoma cruzi infection in Santa Catarina State, southern Brazil. A 63-year-old female had presumptive clinical diagnosis of Chagas' disease, which was confirmed by imaging (endoscopy and esophagogram) and immunological methods. Further molecular diagnosis was carried out with esophagus and blood samples collected during corrective surgery. Polymerase chain reaction tested positive for Trypanosoma cruzi in both esophagus and buffy coat samples.
Asunto(s)
Enfermedad de Chagas/diagnóstico , Acalasia del Esófago/parasitología , Trypanosoma cruzi , Animales , ADN Protozoario/análisis , Acalasia del Esófago/diagnóstico , Femenino , Humanos , Persona de Mediana Edad , Reacción en Cadena de la PolimerasaRESUMEN
We report the first case of digestive tract pathology (megaesophagus) determined by Trypanosoma cruzi infection in Santa Catarina State, southern Brazil. A 63-year- old female had presumptive clinical diagnosis of Chagas' disease, which was confirmed by imaging (endoscopy and esophagogram) and immunological methods. Further molecular diagnosis was carried out with esophagus and blood samples collected during corrective surgery. Polymerase chain reaction tested positive for Trypanosoma cruzi in both esophagus and buffy coat samples
Asunto(s)
Humanos , Femenino , Animales , Persona de Mediana Edad , Enfermedad de Chagas , Acalasia del Esófago , Trypanosoma cruzi , ADN Protozoario , Acalasia del Esófago , Reacción en Cadena de la PolimerasaRESUMEN
The objectives of this study were to analyze the population structure and genetic variability of two communities, Costa da Lagoa (CLG) and São João do Rio Vermelho (SJRV), located on Santa Catarina Island in southern Brazil. The two populations descend from Azores Archipelago immigrants (Portuguese), with a minor contribution of sub-Saharan Africans and Amerindians. To estimate the relative contribution of the different ethnic groups to the current gene pool of the two communities, values of admixture were obtained using the weighted least-squares method based on allelic frequencies of the loci ABO, RHD-RHCE, GPA-GPB (MNSs), HBB, HP, TF, CP, AK, and ACP1. The origins of the studied populations can be quantified as follows: for CLG, sub-Saharan Africans (A) = 17.3%, Iberian Europeans (P) = 75.0%, and Southern Amerindians (I) = 7.7%; for SJRV, A = 48.8%, P = 44.5%, and I = 6.7%. Because haplotype frequencies of the GPA-GPB loci in SJRV were unusual, possibly as a consequence of random genetic drift, the values of admixture were recalculated after exclusion of GPA-GPB, as follows: A = 28.0%; P = 53.3%, and I = 18.7%. The total diversity (HT) was estimated as 42.29%, of which 99.6% can be attributed to the intrapopulational variability (HS). The interpopulational genetic variation (or standard distance, DST) corresponds to 0.19%, while the gene differentiation coefficient is 0.28%, indicative of low genetic difference. These results led to the conclusion that random genetic drift may have had an important effect on the Costa da Lagoa community, while presently gene flow might be the predominant evolutionary factor potentially capable of changing allele frequencies in SJRV.