RESUMEN
This systematic review and meta-analysis estimated the global, regional prevalence, and risk factors of osteoporosis. Prevalence varied greatly according to countries (from 4.1% in Netherlands to 52.0% in Turkey) and continents (from 8.0% in Oceania to 26.9% in Africa). Osteoporosis is a common metabolic bone disorder in the elderly, usually resulting in bone pain and an increased risk of fragility fracture, but few summarized studies have guided global strategies for the disease. Therefore, we pooled the epidemiologic data to estimate the global, regional prevalence, and potential risk factors of osteoporosis. We conducted a comprehensive literature search through PubMed, EMBASE, Web of Science, and Scopus, to identify population-based studies that reported the prevalence of osteoporosis based on the World Health Organization (WHO) criteria. Meta-regression and subgroup analyses were used to explore the sources of heterogeneity. The study was registered in the PROSPERO database (CRD42021285555). Of the 57,933 citations evaluated, 108 individual studies containing 343,704 subjects were included. The global prevalence of osteoporosis and osteopenia was 19.7% (95%CI, 18.0%-21.4%) and 40.4% (95%CI, 36.9%-43.8%). Prevalence varied greatly according to countries (from 4.1% in Netherlands to 52.0% in Turkey) and continents (from Oceania 8.0% to 26.9% in Africa). The prevalence was higher in developing countries (22.1%, 95%CI, 20.1%-24.1%) than in developed countries (14.5%, 95%CI, 11.5%-17.7%). Our study indicates a considerable prevalence of osteoporosis among the general population based on WHO criteria, and the prevalence varies substantially between countries and regions. Future studies with robust evidence are required to explore risk factors to provide effective preventive strategies for the disease.
Asunto(s)
Enfermedades Óseas Metabólicas , Osteoporosis , Anciano , Enfermedades Óseas Metabólicas/etiología , Salud Global , Humanos , Osteoporosis/diagnóstico , Osteoporosis/epidemiología , Osteoporosis/etiología , Prevalencia , Factores de Riesgo , Organización Mundial de la SaludRESUMEN
OBJECTIVE: The study was aimed to investigate the expression of doublecortin-like kinase-1 (DCLK1) in breast cancer (BCa) tissues and cells and further study its association with clinicopathology and prognosis of BCa patients. PATIENTS AND METHODS: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to examine the expression of DCLK1 in 44 BCa tumor tissues, as well as adjacent normal tissues. Also, the interplay between DCLK1 level and clinical data or the prognosis of BCa patients was analyzed. QRT-PCR was further used to verify the level of DCLK1 in BCa cell lines. In addition, the DCLK1 knockdown model was constructed using lentivirus in BCa cell lines. Next, cell counting kit-8 (CCK-8) and cell clone formation and tranwell assays were used to analyze the effect of DCLK1 on the biological function of BCa cells. Finally, it was explored whether DCLK1 can act through the Wnt/ß-Catenin signaling pathway. RESULTS: In this research, qRT-PCR results revealed that the level of DCLK1 in BCa tumor tissues was remarkably higher than in adjacent tissues. Compared to patients with a low-level of DCLK1, the pathology grading in patients with high-level was higher and the overall survival rate was lower. Similarly, proliferation, as well as the invasion and migration ability of cells in DCLK1 knockdown group was remarkably down-regulated when compared to negative control group. Moreover, the Western Blot results revealed that silencing DCLK1 remarkably decreased the expression of key proteins in Wnt/ß-Catenin pathways such as ß-Catenin, c-myc, and cyclinD1, thereby promoting the malignant progression of BCa. In addition, the Wnt/ß-Catenin pathway inhibitor was found to be able to reverse the impact of DCLK1 overexpression on BCa cell proliferative and metastatic capacity. CONCLUSIONS: DCLK1 expression was found remarkably increased in BCa tissues and closely associated with the pathological stage, as well as poor prognosis of BCa patients. Furthermore, DCLK1 may promote the malignant progression of BCa by inhibiting the Wnt/ß-Catenin pathway.