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Genet Mol Res ; 14(3): 7811-20, 2015 Jul 14.
Artículo en Inglés | MEDLINE | ID: mdl-26214462

RESUMEN

Heat shock protein 90 (Hsp90) is a protein produced by plants in response to adverse environmental stresses. In this study, we identified and analyzed Hsp90 gene family members using a bioinformatic method based on genomic data from tomato (Solanum lycopersicum L.). The results illustrated that tomato contains at least 7 Hsp90 genes distributed on 6 chromosomes; protein lengths ranged from 267-794 amino acids. Intron numbers ranged from 2-19 in the genes. The phylogenetic tree revealed that Hsp90 genes in tomato (Solanum lycopersicum L.), rice (Oryza sativa L.), and Arabidopsis (Arabidopsis thaliana L.) could be divided into 5 groups, which included 3 pairs of orthologous genes and 4 pairs of paralogous genes. Expression analysis of RNA-sequence data showed that the Hsp90-1 gene was specifically expressed in mature fruits, while Hsp90-5 and Hsp90-6 showed opposite expression patterns in various tissues of cultivated and wild tomatoes. The expression levels of the Hsp90-1, Hsp90-2, and Hsp90- 3 genes in various tissues of cultivated tomatoes were high, while both the expression levels of genes Hsp90-3 and Hsp90-4 were low. Additionally, quantitative real-time polymerase chain reaction showed that these genes were involved in the responses to yellow leaf curl virus in tomato plant leaves. Our results provide a foundation for identifying the function of the Hsp90 gene in tomato.


Asunto(s)
Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Proteínas HSP90 de Choque Térmico/genética , Familia de Multigenes , Proteínas de Plantas/genética , Solanum lycopersicum/genética , Arabidopsis/genética , Cromosomas de las Plantas/genética , Regulación del Desarrollo de la Expresión Génica , Proteínas HSP90 de Choque Térmico/metabolismo , Calor , Solanum lycopersicum/crecimiento & desarrollo , Oryza/genética , Filogenia , Proteínas de Plantas/metabolismo , Estrés Fisiológico/genética
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