RESUMEN
In an attempt to clarify the nature of analgesic effect of alpha-tocopherol, the influence of alpha-tocopherol on the levels of endogenic opioids was studied in the experiments in vitro. Main changes of the level of adenohypophyseal (tissue) beta-endorphins and in the incubation media were evaluated as well as the involvement of the endogenous opioid system in the analgesic effect of alpha-tocopherol.
Asunto(s)
Adenohipófisis/análisis , Vitamina E/farmacología , betaendorfina/análisis , Animales , Dismenorrea/tratamiento farmacológico , Femenino , Técnicas In Vitro , Ratas , Ratas Endogámicas , Vitamina E/uso terapéuticoRESUMEN
Stress is shown to induce at first the generalized inhibition of lipid peroxidation (LPO), and then the activation of LPO. In brain and blood serum of rats subjected to continuous footshock as well as to restraint stress LPO products decreased and superoxide scavenging activity increased during the initial period of stress, after 1 hour of footshock LPO indices nearly reached normal values, and after 2 hours of footshock the accumulation of LPO products and decrease of superoxide scavenging activity were seen. LPO inhibition was accompanied by accumulation of easy oxidizable brain phospholipids and by depletion of brain cholesterol, during LPO activation brain cholesterol content and cholesterol-phospholipid ratio increased. The content of LPO products--fluorescent Schiff bases in blood plasma of women suffering from algomenorrhea at first decreased (O-12 h) and then dramatically increased (12-24 h) after a onset of pain at the beginning of menstruation. The data suggest that the stage of LPO inhibition precedes its activation during stress.
Asunto(s)
Peroxidación de Lípido , Estrés Fisiológico/metabolismo , Enfermedad Aguda , Animales , Encéfalo/metabolismo , Dismenorrea/sangre , Femenino , Humanos , Fase Luteínica , Dolor/metabolismo , Ratas , Restricción Física , Factores de TiempoRESUMEN
Beta-endorphin-like immunoreactivity was studied in 7 patients with algomenorrhea during pain attack and 15 minutes after alpha-tocopherol administration with a therapeutic aim (till the analgetic effect was reached). There was an increase in beta-endorphin-like immunoreactivity after alpha-tocopherol administration. Naloxone administration to 9 patients with algomenorrhea of various etiology resumed the pain. The effect of alpha-tocopherol application for pain relief depended on the pathogenesis of algomenorrhea. At the same time naloxone administration failed to resume the pain in patients, in whom alpha-tocopherol had a strong analgetic effect. It is assumed that the endogenous opioid system participates in alpha-tocopherol effect on pain relief in patients with algomenorrhea.