RESUMEN
We propose the modified model of the structure of coat protein (CP) subunits in filamentous virions of potato virus X (PVX). The model is similar to the one proposed by us in 2001 for the CP of another helical plant virus (potato virus A) belonging to other (potyvirus) group. In this model the PVX CP molecule consist of two main domains--a bundle of four alpha-helices located close to the virion long axis and a so-called RNP-fold (or abCd-fold) located near the virion surface. Basing on this model we suggest possible mechanism of described by J.G. Atabekov and colleagues structural transition ("remodeling") of the PVX virions resulting from their interaction with virus-specific TGB-1 protein.
Asunto(s)
Proteínas de la Cápside/química , Modelos Químicos , Estructura Terciaria de Proteína , Subunidades de Proteína/químicaRESUMEN
It has been shown by biochemical analysis and differential scanning calorimetry that the connective tissue formed around a transplant as a result of sclero-reinforcing interference (capsula) is similar to intact sclera. The main component of newly formed capsules is collagen I whose fibers have a perfect structure and the amount of cross-links sufficient to provide normal thermomechanical properties. A fraction of collagen having thermally labile "immature" cross-links in capsules formed around the transplant impregraned with Panaxal has been detected by differential scanning calorimetry. It was suggested that fibroblasts in tissues of these capsules have a high synthetic activity.
Asunto(s)
Tejido Conectivo/química , Éteres/química , Prótesis e Implantes , Esclerótica/química , Esclerótica/cirugía , Animales , Rastreo Diferencial de Calorimetría , Colágeno Tipo I/análisis , Fibroblastos/química , Calor , Conejos , TemperaturaRESUMEN
Mutant ts21-66 of the tobacco mosaic virus (TMV) differs from the wild-type TMV-U1 by two mutations (Ile-21-->Thr and Asp-66-->Gly) in the coat protein (CP) gene and in symptoms produced in infected N' plants. The CP structure in TMV-U1 and ts21-66 virions was probed by tritium planigraphy. Compared with the wild-type CP, labeling of the N-terminal region of mutant CP was half as high and suggested its greater shielding. A role of this CP region in virus interactions with the N' resistance system is discussed.
Asunto(s)
Virus del Mosaico del Tabaco/química , Proteínas del Envoltorio Viral/química , Secuencia de Aminoácidos , Cromatografía Líquida de Alta Presión , Modelos Moleculares , Datos de Secuencia Molecular , Mutación , Conformación Proteica , Resonancia por Plasmón de Superficie , Virus del Mosaico del Tabaco/genéticaRESUMEN
The emergency operations were performed in 6,506 patients for acute hemorrhage in gastric and duodenal ulcer. A method for the complex corrigative intensive therapy is presented. The use of combined parenteral and enteral nutrition facilitates a care of the patients, favours the prophylaxis of complications. Because of incomplete rehabilitation by the moment of patient's discharge from the hospital, it is necessary to continue the corrigative therapy directed at liquidation of the globular volume deficiency, correction of disproteinemia, normalization of the indices of central hemodynamics and metabolic processes in the organs and tissues.