RESUMEN
Chemical oxygen demand (COD) is a synthetical indicator which represents the degree of organic pollution in water. The near-infrared (NIR) transmission and the UV absorbance method based on photoelectric detection technology and spectroscopy analysis have some advantages such as high precision, speed, non-contact, no secondary pollution etc compared to conventional wet chemical method. The NIR transmission spectra and UV absorbance spectra of standard solution configured with phthalate hydrogen potassium were collected respectively by MPA FTIR spectrometer (Bruker Optics Inc.) made in Germany and AvaSpec-2048-2 UV spectrometer (Avantes Inc.) made in Netherlands. After different pretreatment to the spectra, COD quantitative analysis model was established using partial least squares regression (PLS) and linear regression. The statistical analysis of COD quantitative model was implemented, and the result showed that UV absorbance method had a higher relevance but lower forecast accuracy and precision than NIR transmission method.
RESUMEN
The properties of room temperature phosphorescence (RTP) of the labeled goat anti-human antibody(with fluorescein isothiocynate GAHAb-FITC) and labeled rabit anti-goat antibody(RAGAb-FITC) with FITC in different dilution ratios on many kinds of solid substrates were studied in detail. It is found that on the polyamide membrane, only using Pb(Ac)2 as a heavy atom perturber, both the labeled antibodies and their antigen-antibody conjugated compound with the human immunoglobulin G remained excellent RTP properties of isothiocyanate fluorescein, lambda ex(max)/lambda em(max) = 525/650 nm, the relation of RTP intensity to the concentration was linear in a certain range. The conditions for immunological reaction and RTP emission were optimized. The result means that it is possible to develop a new immunoassay named as solid substrate room temperature phosphorescence immunoassay (SS-RTP-IA), which combines both the specificity of immunological reaction and high sensitivity of luminescence analysis.