RESUMEN

OBJECTIVES: This study aims to assess the outcome of total hip arthroplasty (THA) using artificial intelligence (AI)-assisted three-dimensional (3D) preoperative planning technology in terms of predicting prosthesis size, acetabular cup positioning, and lowerlimb length restoration. PATIENTS AND METHODS: Between January 2020 and July 2022, a total of 161 patients (101 males, 60 females; mean age: 57.6±10.5 years; range, 31 to 80 years) who underwent primary unilateral THA were retrospectively analyzed. The patients were divided into two groups as those who were treated with AI-assisted 3D preoperative planning technology (the observation group, n=95) and patients who were treated with traditional two-dimensional (2D) X-ray template planning technology (the control group, n=66). RESULTS: The accuracy of the planning was based on the consistency of the preoperative planning and intraoperative models. The difference between the observation group and the control group was statistically significant in terms of the accuracy of the preoperative planning of acetabular prostheses (54% vs. 38%, p=0.048) and femoral prostheses (64% vs. 44%, p=0.011), with both values significantly higher in the observation group. The mean inclination angle, anteversion angle, and limb length discrepancy (LLD) in the observation group were 36.85°±4.82°, 12.10°±5.33°, and 2.18±2.70 mm, respectively, while those in the control group were 35.06°±6.07°, 10.95°±5.09°, and 4.42±3.85 mm, respectively. There was a statistically significant difference between the two groups in terms of inclination angle and LLD (p<0.05 for both), but there was no significant difference in terms of anteversion angle (p>0.05). In the observation group, 86.3% (82/95) of acetabular cups were implanted within the Lewinnek safe zone (72.7% [48/66] in the control group), while 83.2% (79/95) were within the Callanan safe zone (69.7% [46/66] in the control group), with both values higher in the observation group (p<0.05). CONCLUSION: Overall, AI-assisted 3D preoperative planning is superior to traditional 2D X-ray template planning for predicting prosthesis size, and it also has the advantage in terms of acetabular cup positioning and lower-limb length restoration.

Asunto(s)

Artroplastia de Reemplazo de Cadera , Prótesis de Cadera , Femenino , Masculino , Humanos , Persona de Mediana Edad , Anciano , Inteligencia Artificial , Estudios Retrospectivos , Acetábulo/diagnóstico por imagen , Acetábulo/cirugía

RESUMEN

OBJECTIVES: This study aims to assess the short-term outcome of total hip arthroplasty for treating developmental dysplasia of the hip (DDH) using artificial intelligence (AI)-assisted three-dimensional (3D) preoperative planning technology. PATIENTS AND METHODS: Between January 2020 and July 2022, a total of 61 patients with DDH (31 males, 30 females, mean age: 59.2±10.4 years; range, 35 to 78 years) were retrospectively analyzed. The patients were divided into two groups as those in the observation group of AI-assisted 3D preoperative planning technology (n=34) and the control group of traditional two-dimensional X-ray template planning technology (n=27). Perioperative data of the patients were recorded and analyzed. RESULTS: All patients were followed for more than one year, and no hip dislocation, aseptic loosening, periprosthetic fracture, periprosthetic infection or revision occurred. The accuracy of the planning was based on the agreement between the preoperative planning model and the intraoperative model. The accuracy of preoperative planning for the acetabular prosthesis and femoral prosthesis in the observation group was significantly higher than in the control group. No statistically significant difference was found in the postoperative abduction (p=0.416) and anteversion (p=0.225) between the groups. In the observation group, 91.2% of the acetabular cups were implanted within the Lewinnek safe zone (66.7% in the control group) and 88.2% were within the Callanan safe zone (63% in the control group). There was a statistically significant difference between the two groups in terms of the postoperative lower-limb length discrepancy (p=0.004), which was significantly improved in both groups compared to preoperative values (p<0.01 for all). The postoperative Harris hip score in both groups was significantly improved compared to preoperative scores (p<0.01); however, there was no statistically significant difference between the two groups (p=0.098). CONCLUSION: Our study results suggest that AI-assisted 3D preoperative planning is evidently more successful than traditional 2D X-ray template planning for predicting prosthesis size. This method seems to be advantageous in acetabular cup positioning, as well as in lower-limb length restoration.

Asunto(s)

Artroplastia de Reemplazo de Cadera , Displasia del Desarrollo de la Cadera , Prótesis de Cadera , Femenino , Masculino , Humanos , Persona de Mediana Edad , Anciano , Inteligencia Artificial , Estudios Retrospectivos

RESUMEN

Glucocorticoid-induced osteoporosis is characterized by osteoblastic cell and microarchitecture dysfunction, as well as a loss of bone mass. Cell senescence contributes to the pathological process of osteoporosis and sodium hydrosulfide (NaHS) regulates the potent protective effects through delaying cell senescence. The aim of the present study was to investigate whether senescence could contribute to dexamethasone (Dex)-induced osteoblast impairment and to examine the effect of NaHS on Dex-induced cell senescence and damage. It was found that the levels of the senescence-associated markers, p53 and p21, were markedly increased in osteoblasts exposed to Dex. A p53 inhibitor reversed Dex-induced osteoblast injury, a process that was mitigated by NaHS administration through alleviating osteoblastic cell senescence. MicroRNA (miR)-22 blocked the impact of NaHS on Dex-induced osteoblast damage and senescence through targeting the regulation of Sirtuin 1 (sirt1) expression, as shown by the decreased cell viability and alkaline phosphatase activity, as well as an increased expression of p53 and p21. It was revealed that the sirt1 gene was the target of miR-22 in osteoblastic MC3T3-E1 cells through combining the results of dual luciferase reporter assays and reverse transcription-quantitative PCR, as well as western blot analyses. Silencing of sirt1 abolished the protective effect of NaHS against Dex-associated osteoblast senescence and injury. Taken together, the present study showed that NaHS prevents Dex-induced cell senescence and damage through targeting the miR-22/sirt1 pathway in osteoblastic MC3T3-E1 cells.

RESUMEN

The present study aimed to explore the therapeutic effects of cyclosporin A (CsA) on spinal cord injury (SCI) in rats with hyperglycemia and to identify a novel potential method to treat SCI in the presence of hyperglycemia. Female Sprague­Dawley (SD) rats were randomly allocated into four groups: Sham, SCI, SCI+hyperglycemia and SCI+hyperglycemia+CsA groups. Streptozotocin­induced hyperglycemic SD rats and a weight­drop contusion SCI model were established. The Basso, Beattie, Bresnahan scale and inclined plane test were used to evaluate the neurological function of the rats. Flow cytometric assay was performed to detect the apoptotic rates of cells in the spinal cord. ELISA and western blot analysis were performed to determine the levels of interleukin (IL)­10, tumor necrosis factor (TNF)­α, cyclophilin­D (Cyp­D) and apoptosis­inducing factor (AIF). The results demonstrated that CsA significantly improved the neurological function of the SCI rats with hyperglycemia. CsA markedly reduced the number of apoptotic cells exaggerated by hyperglycemia in the spinal cord of the SCI rats. CsA significantly decreased the expression levels of IL­10, TNF­α, Cyp­D and AIF in the spinal cord of the SCI rats. Overall, the present study revealed a significant role of CsA in the treatment of SCI in the presence of hyperglycemia by inhibiting the apoptosis of spinal cord cells.

Asunto(s)

Ciclosporina/farmacología , Hiperglucemia/tratamiento farmacológico , Fármacos Neuroprotectores/farmacología , Recuperación de la Función/efectos de los fármacos , Traumatismos de la Médula Espinal/tratamiento farmacológico , Médula Espinal/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Factor Inductor de la Apoptosis/genética , Factor Inductor de la Apoptosis/metabolismo , Peptidil-Prolil Isomerasa F , Ciclofilinas/genética , Ciclofilinas/metabolismo , Modelos Animales de Enfermedad , Femenino , Regulación de la Expresión Génica , Hiperglucemia/inducido químicamente , Hiperglucemia/complicaciones , Hiperglucemia/genética , Interleucina-10/genética , Interleucina-10/metabolismo , Actividad Motora/efectos de los fármacos , Actividad Motora/fisiología , Regeneración Nerviosa/efectos de los fármacos , Regeneración Nerviosa/fisiología , Neuroglía/efectos de los fármacos , Neuroglía/metabolismo , Neuroglía/patología , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Neuronas/patología , Ratas , Ratas Sprague-Dawley , Recuperación de la Función/fisiología , Médula Espinal/metabolismo , Médula Espinal/patología , Traumatismos de la Médula Espinal/complicaciones , Traumatismos de la Médula Espinal/genética , Traumatismos de la Médula Espinal/patología , Estreptozocina , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/metabolismo

RESUMEN

Creative arts therapies are proven to promote an interconnection between body and mind, but there are major obstacles for providing therapeutic services in prisons due to inmates' inherent mistrust for verbal disclosure and rigid self-defenses, especially among inmates with schizophrenia. Thus, we developed a structured and quantitative art brut therapy program called go beyond the schizophrenia to actually measure the benefits of art therapy on prison inmates in mainland China. Upon completion of the program, the intervention group reported a decrease in anxiety, depression, anger, and negative psychiatric symptoms and showed better compliance with rules, socialization with peers, compliance with medications, and regular sleeping patterns after 16 weekly sessions of go beyond the schizophrenia. This article concludes that the art brut therapy was effective for the inmates with schizophrenia in mainland China and provides encouraging data on how to enhance mental health for inmates with schizophrenia. KEY PRACTITIONER MESSAGE: Art brut therapy can reduce emotional distress and negative psychiatric symptoms among Chinese inmates. Arts brut therapy can enhance Chinese inmates' compliance with rules, socialization with peers, compliance with medicines, and regular sleeping patterns. Arts brut therapy in conjunction with medication is highly recommended for recovery of Chinese inmates with schizophrenia, especially for patients with negative symptoms.

Asunto(s)

Arteterapia/métodos , Prisioneros/psicología , Esquizofrenia/terapia , Psicología del Esquizofrénico , Adulto , China , Femenino , Humanos , Estudios Longitudinales , Masculino , Persona de Mediana Edad , Prisioneros/estadística & datos numéricos , Resultado del Tratamiento

RESUMEN

OBJECTIVE: To elucidate the effect of transforming growth factor-beta 1 (TGF-ß1) on peritoneal fibrosis and the regulation of gastric cancer adhering to mesothelial cells. METHODS: The peritoneal mesothelial cell line of HMrSV5 was used to determine the role of TGF-ß1 in the regulation of gastric cancer cell adhering to mesothelial cells. And the mRNA and protein expressions of collagen III and fibronectin were detected by adhesion assay, Western blot, immunofluorescent staining and reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: (1) The treatment of 5 ng/ml TGF-ß1 could induce the expressions of collagen III and fibronectin in mesothelial cells at 24, 48 and 72 h (P < 0.01). (2) As compared with the controls, the percentages of adhered HGC-27 and HSC-39 gastric cancer cells significantly increased under the treatment of TGF-ß1 for 24 and 72 h. The increased adhesion percentages of HGC-27 were 65% ± 5% and 124% ± 11% (P < 0.05) while those of HSC-39 85% ± 9% and 146% ± 17% respectively (P < 0.05). (3) Arginyl-glycyl-aspartic acid (RGD) (knockdown of minimal sites for cell-binding domain of extracellular matrix) decreased the number of cancer cells adhering to mesothelial cells under the stimulation of TGF-ß1. And the decreased adhesion percentage of HGC-27 was 65% ± 8% (P < 0.05). CONCLUSIONS: TGF-ß1 significantly stimulates the expressions of collagen III and fibronectin in mesothelial cells. And it is associated with the increased adhesion of gastric cancer cell and offers a favorable environment for the dissemination of gastric cancer.

Asunto(s)

Células Epiteliales/efectos de los fármacos , Fibrosis Peritoneal/patología , Neoplasias Gástricas/patología , Factor de Crecimiento Transformador beta1/farmacología , Adhesión Celular/efectos de los fármacos , Colágeno Tipo III/metabolismo , Células Epiteliales/citología , Fibronectinas/metabolismo , Humanos , Fibrosis Peritoneal/metabolismo , Peritoneo/citología , Neoplasias Gástricas/metabolismo , Células Tumorales Cultivadas