RESUMEN
Ammonia loss is the most severe during the high-temperature stage (>50°C) of aerobic composting. Regulating ammonia volatilization during this period via thermophilic microbes can significantly improve the nitrogen content of compost and reduce air pollution due to ammonia loss. In this study, an ammonia-assimilating bacterial strain named LL-8 was screened out as having the strongest ammonia nitrogen conversion rate (32.7%) at high temperatures (50°C); it is able to significantly reduce 42.9% ammonia volatile loss in chicken manure composting when applied at a high-temperature stage. Phylogenetic analysis revealed that LL-8 was highly similar (>98%) with Priestia aryabhattai B8W22T and identified as Priestia aryabhatta. Genomic analyses indicated that the complete genome of LL-8 comprised 5,060,316 base pairs with a GC content of 32.7% and encoded 5,346 genes. Genes, such as gudB, rocG, glnA, gltA, and gltB, that enable bacteria to assimilate ammonium nitrogen were annotated in the LL-8 genome based on the comparison to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The results implied that the application of thermophilic ammonia-assimilating strain P. aryabhatta LL-8 would be a promising solution to reduce ammonia loss and mitigate air pollution of aerobic composting.IMPORTANCEAerobic composting is one of the essential ways to recycle organic waste, but its ammonia volatilization is severe and results in significant nitrogen loss, especially during the high-temperature period, which is also harmful to the environment. The application of thermophilic bacteria that can use ammonia as a nitrogen source at high temperatures is helpful to reduce the ammonia volatilization loss of composting. In this study, we screened and identified a bacteria strain called LL-8 with high temperature (50°C) resistance and strong ammonia-assimilating ability. It also revealed significant effects on decreasing ammonia volatile loss in composting. The whole-genome analysis revealed that LL-8 could utilize ammonium nitrogen by assimilation to decrease ammonia volatilization. Our work provides a theoretical basis for the application of this functional bacteria in aerobic composting to control nitrogen loss from ammonia volatilization.
RESUMEN
Flue-cured tobacco is one of the important sources of national economy in China. However, Phthalic acid esters (PAEs) are ubiquitous contaminants in the cultivation and growth management of flue-cured tobacco, and attracting more and more attention. Here, six priority PAEs were detected in tobacco and soils and their residue characteristics, pollution sources were analyzed, and their exposure risks to the health of farmers were assessed. The concentration of six total PAEs ranged from 0.78 to 4.79 mg/kg in tobacco with the average of 1.75 mg/kg, and 0.84-25.68 mg/kg in soils with the average of 5.40 mg/kg. Di-(2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DBP) had the highest detection frequency (DF = 100%) both in soil and tobacco samples. DEHP was the most abundant of the total PAEs in soil and tobacco samples, with the mean contribution values of 71.0% and 58.8%, respectively. Principal component analysis (PCA) indicates that the major sources of PAEs in the tobacco-soil system were plastic films, fertilizers and pesticides. Health risk assessment suggests that the non-cancer hazard indexes (NCHI) of dimethyl phthalate (DMP), diethyl phthalate (DEP), DBP and di-n-octyl phthalate (DnOP) in all samples for farmers were at acceptable levels (NCHI < 1), and the average carcinogenic hazard indexes (CHI) of butyl benzyl phthalate (BBP) and DEHP for farmers were 3.79 × 10-13 and 8.54 × 10-11 in soils, respectively, 8.23 × 10-13 and 1.95 × 10-11 in tobacco, respectively, which were considered to be very low level (CHI < 10-6). This study provides data on PAEs in tobacco and soils and their health risks which may provide valuable information to aid the management of tobacco cultivation and risk avoidance.
Asunto(s)
Dietilhexil Ftalato , Ácidos Ftálicos , Contaminantes del Suelo , China , Dibutil Ftalato/análisis , Dietilhexil Ftalato/análisis , Ésteres/análisis , Ácidos Ftálicos/análisis , Medición de Riesgo , Suelo/química , Contaminantes del Suelo/análisis , NicotianaRESUMEN
Plant-plant interactions is one of the key field in ecology, which is important for the efficient nutrient utilization, productivity improvement, and plant community assembly. Arbuscular mycorrhizal fungi are important plant mutualistic microorganisms that connect plant roots to form common mycelial networks (CMNs), which play major roles in transferring nutrients and water and regu-lating plant community dynamics. Recent studies demonstrated that these CMNs could act as conduits for transmitting disease and aphid-induced signals among plants, and activating chemical defence in uninfested neighboring plants. In this review, we introduced recent research advances on the contribution of CMNs on plant interaction, the main factors that influences the functions of CMNs, and the role of CMNs transfer and redistribute nutrients and water among plant. In addition, the mechanism underlying underground chemical signal communication, seedling establishment and plants community assembly were summarized. Finally, we proposed challenges facing CMNs in plant-plant interactions and the practical problems. It would provide reference for further understanding the ecological functions of CMNs in plant-plant interactions.