RESUMEN
The level of circulating cortisol and peripheral blood parameters were determined in carp age 2 years (K(2)) 24, 72, and 216 h after a single intraperitoneal injection of a high dose (200 mg x kg(-1) body wt) of hydrocortisone. The most striking effect of cortisol was manifest as a significant change in the percentage composition of leukocytes, whose number per unit volume of blood remained relatively constant. A profound lymphopenia and eosinopenia were compensated for in the general balance by an increased number of circulating promyelocytes and myelocytes as well as metamyelocytes and mature polymorphonuclear neutrophilic granulocytes. The results and their possible reasons are discussed on the background of literature data.
Asunto(s)
Carpas/sangre , Hidrocortisona/administración & dosificación , Hidrocortisona/sangre , Leucocitos/efectos de los fármacos , Animales , Eosinófilos , Granulocitos , Células Madre Hematopoyéticas , Hidrocortisona/farmacología , Inyecciones Intraperitoneales , Recuento de Leucocitos , Linfocitos , NeutrófilosRESUMEN
The activities of Fru-1,6-P2ase and G-6-Pase in liver and kidney of frogs acclimated at 5 and 25 degrees C and Fru-2,6-P2 level in liver were investigated. The aim of this study was to examine the effect of thermal acclimation on regulatory enzymes of gluconeogenesis and on concentration of gluconeogenesis regulator. Fru-1,6-P2ase activity in liver of frogs acclimated at 5 degrees C was 6.16 +/- 0.77 and 4.46 +/- 0.46 U/g wt in those acclimated at 25 degrees C; the respective values for G-6-Pase were 0.46 +/- 0.04 and 0.25 +/- 0.02 U/g wt. Fru-1,6-P2ase activity in kidney was 3.2 +/- 0.48 U/g wt at 5 degrees C and 2.64 +/- 0.23 U/g wt at 25 degrees C; the respective values for G-6-Pase were 0.2 +/- 0.05 and 0.17 +/- 0.05 U/g wt. K(m) of frog liver Fru-1,6-P2ase determined after acclimation at 5 degrees C and to 25 degrees C was 1.36 and 1.41 microM, respectively. Frog liver Fru-1,6-P2ase was allosterically inhibited by AMP. I0.5 determined after acclimation at 5 degrees C was 10.55 microM and after acclimation at 25 degrees C was 10.88 microM. Liver Fru-2,6-P2 concentration after acclimation at 5 degrees C was 0.44 +/- 0.13 nmol/g wt in comparison with 0.58 +/- 0.19 nmol/g wt after acclimation at 25 degrees C. In conclusion, cold exposure increased hepatic gluconeogenic capacity of Rana esculenta.
Asunto(s)
Aclimatación/fisiología , Fructosa-Bifosfatasa/metabolismo , Fructosadifosfatos/metabolismo , Glucosa-6-Fosfatasa/metabolismo , Rana esculenta/metabolismo , Adenosina Monofosfato/farmacología , Regulación Alostérica , Animales , Glucemia/metabolismo , Inhibidores Enzimáticos/farmacología , Fructosa-Bifosfatasa/antagonistas & inhibidores , Gluconeogénesis , Riñón/metabolismo , Cinética , Hígado/metabolismo , TemperaturaRESUMEN
The activity of aspartate aminotransferase and alanine aminotransferase and the content of soluble proteins were determined in mice irradiated with single dose of 100 R or injected with turpentine and in mice subjected to both these stress factors. The aim of this study was determination of changes in the activity of both these enzymes in the liver, kidney and spleen within 48 hours). It was found that the action of both these stress factors caused significant changes in the activity of AspAT and A1AT in the first phase of the response of the organism to stress and caused statistically significant changes of this activity on the second day of the experiment.