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Sarcopenia is a significant health concern primarily affecting old adult individuals, characterized by age-related muscle loss, and decreased strength, power, and endurance. It has profound negative effects on overall health and quality of life, including reduced independence, mobility, and daily activity performance, osteoporosis, increased fall and fracture risks, metabolic issues, and chronic diseases like diabetes and cardiovascular conditions. Preventive strategies typically involve a combination of proper nutrition and regular physical activity. Among strength training exercises, high-intensity interval training (HIIT) stands out as the most effective approach for improving muscle function in older adults with sarcopenia. The current review identifies and summarizes the studies that have examined the effects of HIIT on muscle strength in older adults as an element of the prevention and treatment of sarcopenia. A systematic search using several computerized databases, namely, MEDLINE/PubMed, Scopus, SPORTDiscus, and Web of Science, was performed on 12 January 2023, according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. A total of 224 studies were initially retrieved. A total of five studies met the selection criteria. HIIT training shows improvements in body composition and functional and cardiorespiratory capacity, has benefits on muscle strength, increases muscle quality and architecture, and is associated with muscle hypertrophy in healthy older adults. Nonetheless, given the shortcomings affecting primary research in terms of the limited number of studies and the high risk of bias, further research is warranted.

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Due to the high concentration of proteins, nucleic acids, and other macromolecules, the bacterial cytoplasm is typically described as a crowded environment. However, the extent to which each of these macromolecules individually affects the mobility of macromolecular complexes, and how this depends on growth conditions, is presently unclear. In this study, we sought to quantify the crowding experienced by an exogenous 40 nm fluorescent particle in the cytoplasm of E. coli under different growth conditions. By performing single-particle tracking measurements in cells selectively depleted of DNA and/or mRNA, we determined the contribution to crowding of mRNA, DNA, and remaining cellular components, i.e., mostly proteins and ribosomes. To estimate this contribution to crowding, we quantified the difference of the particle's diffusion coefficient in conditions with and without those macromolecules. We found that the contributions of the three classes of components were of comparable magnitude, being largest in the case of proteins and ribosomes. We further found that the contributions of mRNA and DNA to crowding were significantly larger than expected based on their volumetric fractions alone. Finally, we found that the crowding contributions change only slightly with the growth conditions. These results reveal how various cellular components partake in crowding of the cytoplasm and the consequences this has for the mobility of large macromolecular complexes.

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Soccer is a predominantly tactical sport and, therefore, tactical training has become the most widely used strategy to improve players' performance. The objective of the present study was to assess the workload of soccer-specific drills in professional players over a two-season period in an established context. GPS technology was used to record the data. One hundred and thirty-two (n = 132) soccer-specific drills were studied and grouped by categories. The individual demands of each task were related to the individual competitive profile of each player. The level of physical demand was significantly different in relation to the specific soccer drills analysed. Total distance covered, high-speed running, and the total number of high accelerations and decelerations were significantly higher in competition than in drills used for training sessions (p < .001). The Large-Sided Games (LSG), Big-Position Games (BPG) and Position Games (PG) showed higher maximum running speed values than the rest of the exercises (p < .01). The sum of high accelerations and decelerations values was greater in the Small-Sided Games (SSG) than in BPG (p < .001), Small-Position Games (SPG) (p < .001) and Physical-Technical Circuits (PTC) (p < .001). Significant differences were observed in the exercises analysed according to the player's position. The current findings provide a detailed description of conditional demands placed on soccer players in different soccer-specific drills during training sessions, in a professional soccer context and according to their playing position, which may be helpful in the development of individualized training programs in other contexts.

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Based on recent findings indicating that metabolism might be governed by a limit on the rate at which cells can dissipate Gibbs energy, in this Perspective, we propose a new mechanism of how metabolic activity could globally regulate biomolecular processes in a cell. Specifically, we postulate that Gibbs energy released in metabolic reactions is used to perform work, allowing enzymes to self-propel or to break free from supramolecular structures. This catalysis-induced enzyme movement will result in increased intracellular motion, which in turn can compromise biomolecular functions. Once the increased intracellular motion has a detrimental effect on regulatory mechanisms, this will establish a feedback mechanism on metabolic activity, and result in the observed thermodynamic limit. While this proposed explanation for the identified upper rate limit on cellular Gibbs energy dissipation rate awaits experimental validation, it offers an intriguing perspective of how metabolic activity can globally affect biomolecular functions and will hopefully spark new research.

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ABSTRACT: Jiménez-Reyes, P, Garcia-Ramos, A, Párraga-Montilla, JA, Morcillo-Losa, JA, Cuadrado-Peñafiel, V, Castaño-Zambudio, A, Samozino, P, and Morin, J-B. Seasonal changes in the sprint acceleration force-velocity profile of elite male soccer players. J Strength Cond Res 36(1): 70-74, 2022-This study aimed to describe the seasonal changes in the sprint force-velocity (Fv) profile of professional soccer players. The sprint Fv profile of 21 male soccer players competing in the first division of the Spanish soccer league was evaluated 6 times: preseason 1 (September 2015), in-season 1 (November 2015), in-season 2 (January 2016), in-season 3 (March 2016), in-season 4 (May 2016), and preseason 2 (August 2016). No specific sprint capabilities stimuli other than those induced by soccer training were applied. The following variables were calculated from the velocity-time data recorded with a radar device during an unloaded sprint: maximal force (F0), maximal velocity (v0), Fv slope, maximal power (Pmax), decrease in the ratio of horizontal-to-resultant force (DRF), and maximal ratio of horizontal-to-resultant force (RFpeak). F0 (effect size [ES] range = 0.83-0.93), Pmax (ES range = 0.97-1.05), and RFpeak (ES range = 0.56-1.13) were higher at the in-seasons 2 and 3 compared with both preseasons (p ≤ 0.006). No significant differences were observed for v0, Fv slope, and DRF (p ≥ 0.287). These results suggest that relevant Fv profile variables may be compromised (F0 more compromised than v0) toward the end of the competitive season when specific sprint stimuli are not systematically applied.

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Ebola virus disease outbreaks have repeatedly occurred on the African continent over the last decades, with more serious outbreaks in recent years. Being highly transmissible and associated to high fatality rates, it constitutes a serious threat to public health. Vaccination, however, may allow for efficient control of its propagation. The most promising Ebola vaccine candidate to date, rVSV-ZEBOV, relies on a recombinant vesicular stomatitis virus construct, in which the native viral glycoprotein is replaced by the glycoprotein of Ebola virus (Zaire). However, its cell-based manufacturing process is still lengthy and cumbersome, thus urging the implementation of a new and more efficient bioprocess. To address these issues, serum-free production of rVSV-ZEBOV in Vero cells has been studied with the aim to test an alternative upstream process. Until viable options of suspension cell culture are available, Vero cell cultures still rely on adherent bioprocesses and have thus been developed in this work. Particularly, a bioprocess developed with standard microcarrier bioreactor technology was successfully transferred to the novel single-use scale-X™ hydro fixed-bed.

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PURPOSE: To compare the sprint mechanical force-velocity (F-V) profile between soccer and futsal players. A secondary aim was, within each sport, to study the differences in sprint mechanical F-V profile between sexes and players of different levels. METHODS: A total of 102 soccer players (63 men) and 77 futsal players (49 men) who were competing from the elite to amateur levels in the Spanish league participated in this investigation. The testing procedure consisted of 3 unloaded maximal 40-m sprints. The velocity-time data recorded by a radar device were used to calculate the variables of the sprint acceleration F-V profile (maximal theoretical force [F0], maximal theoretical velocity [V0], maximal power [Pmax], decrease in the ratio of horizontal to resultant force [DRF], and maximal ratio of horizontal to resultant force [RFpeak]). RESULTS: Futsal players showed a higher F0 than soccer players (effect size [ES] range: 0.11-0.74), while V0 (ES range: -0.48 to -1.15) and DRF (ES range: -0.75 to -1.45) was higher for soccer players. No significant differences were observed between soccer and futsal players for Pmax (ES range: -0.43 to 0.19) and RFpeak (ES range: -0.49 to 0.30). Men and high-level players presented an overall enhanced F-V profile compared with women and their lower-level counterparts, respectively. CONCLUSIONS: The higher F0 and lower V0 of futsal players could be caused by the game's specific demands (larger number of accelerations but over shorter distances than in soccer). These results show that the sprint mechanical F-V profile is able to distinguish between soccer and futsal players.

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