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1.
Bioresour Technol ; 382: 129176, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-37187334

RESUMEN

To improve the CO2 dissolution and carbon fixation in the process of microalgae capturing CO2 from flue gas, a nanofiber membrane containing iron oxide nanoparticles (NPsFe2O3) for CO2 adsorption was prepared, and coupled with microalgae utilization to achieve carbon removal. The performance test results showed that the largest specific surface area and pore size were 8.148 m2 g-1 and 27.505 Å, respectively, when the nanofiber membrane had 4% NPsFe2O3. Through CO2 adsorption experiments, it was found that the nanofiber membrane could prolong the CO2 residence time and increase CO2 dissolution. Then, the nanofiber membrane was used as a CO2 adsorbent and semifixed culture carrier in the Chlorella vulgaris culture process. The results showed that compared with the group without nanofiber membrane (0 layer), the biomass productivity, CO2 fixation efficiency and carbon fixation efficiency of Chlorella vulgaris with 2 layers of membranes increased by 1.4 times.


Asunto(s)
Chlorella vulgaris , Microalgas , Nanofibras , Dióxido de Carbono , Ciclo del Carbono , Biomasa , Nanopartículas Magnéticas de Óxido de Hierro
3.
PLoS One ; 18(2): e0278568, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36848343

RESUMEN

Green biomass is a renewable and biodegradable material that has the potential use to trap urea to develop a high-efficiency urea fertilizer for crops' better performance. Current work examined the morphology, chemical composition, biodegradability, urea release, soil health, and plant growth effects of the SRF films subjected to changes in the thickness of 0.27, 0.54, and 1.03 mm. The morphology was examined by Scanning Electron Microscopy, chemical composition was analyzed by Infrared Spectroscopy, and biodegradability was assessed through evolved CO2 and CH4 quantified through Gas Chromatography. The chloroform fumigation technique was used for microbial growth assessment in the soil. The soil pH and redox potential were also measured using a specific probe. CHNS analyzer was used to calculate the total carbon and total nitrogen of the soil. A plant growth experiment was conducted on the Wheat plant (Triticum sativum). The thinner the films, the more they supported the growth and penetration of the soil's microorganisms mainly the species of fungus possibly due to the presence of lignin in films. The fingerprint regions of the infrared spectrum of SRF films showed all films in soil changed in their chemical composition due to biodegradation but the increase in the thickness possibly provides resistance to the films' losses. The higher thickness of the film delayed the rate and time for biodegradation and the release of methane gas in the soil. The 1.03 mm film (47% in 56 days) and 0.54 mm film (35% in 91 days) showed the slowest biodegradability as compared to the 0.27 mm film with the highest losses (60% in 35 days). The slow urea release is more affected by the increase in thickness. The Korsymer Pappas model with release exponent value of < 0.5 explained the release from the SRF films followed the quasi-fickian diffusion and also reduced the diffusion coefficient for urea. An increase in the pH and decrease in the redox potential of the soil is correlated with higher total organic content and total nitrogen in the soil in response to amending SRF films with variable thickness. Growth of the wheat plant showed the highest average plant length, leaf area index and grain per plant in response to the increase in the film's thickness. This work developed an important knowledge to enhance the efficiency of film encapsulated urea that can better slow the urea release if the thickness is optimized.


Asunto(s)
Fertilizantes , Películas Cinematográficas , Biodegradación Ambiental , Biomasa , Ligando de CD40
4.
Bioresour Technol ; 370: 128503, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36535615

RESUMEN

This study presented a novel methodology to predict microalgae chlorophyll content from colour models using linear regression and artificial neural network. The analysis was performed using SPSS software. Type of extractant solvents and image indexes were used as the input data for the artificial neural network calculation. The findings revealed that the regression model was highly significant, with high R2 of 0.58 and RSME of 3.16, making it a useful tool for predicting the chlorophyll concentration. Simultaneously, artificial neural network model with R2 of 0.66 and low RMSE of 2.36 proved to be more accurate than regression model. The model which fitted to the experimental data indicated that acetone was a suitable extraction solvent. In comparison to the cyan-magenta-yellow-black model in image analysis, the red-greenblue model offered a better correlation. In short, the estimation of chlorophyll concentration using prediction models are rapid, more efficient, and less expensive.


Asunto(s)
Clorofila , Microalgas , Redes Neurales de la Computación , Modelos Lineales
5.
J Biotechnol ; 360: 11-22, 2022 Dec 10.
Artículo en Inglés | MEDLINE | ID: mdl-36272573

RESUMEN

As the world's population grows, it is necessary to rethink how countries throughout the world produce food in order to replace the conventional and unsustainable agricultural techniques. Microalgae cultivation using a nutrient-rich solution from hydroponic systems not only presents a novel approach to solving problems pertaining to the impact of the discharges on the natural environment but also provides a plethora of other biotechnological applications particularly in the productions of high value-added products and plants growth stimulants, which can be potentially assimilated into the circular bioeconomy (CBE) in the hydroponic sector. In this review, the potential and practicability of microalgae to be merged into hydroponics CBE are reviewed. Overall, the integration of microalgal biorefineries in hydroponics systems can be realized after considering their Technology Readiness Level and System Readiness Level beforehand. Several suggestions on strains and hydroponics system improvement using existing biotechnological tools, Artificial Intelligence (AI) and nanobiotechnology in support of the CBE will be covered.


Asunto(s)
Microalgas , Aguas Residuales , Inteligencia Artificial , Biotecnología
6.
Bioresour Technol ; 365: 128169, 2022 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-36283661

RESUMEN

Overexploitation of natural resources to meet human needs has considerably impacted CO2 emissions, contributing to global warming and severe climatic change. This review furnishes an understanding of the sources, brutality, and effects of CO2 emissions and compelling requirements for metamorphosis from a linear to a circular bioeconomy. A detailed emphasis on microalgae, its types, properties, and cultivation are explained with significance in attaining a zero-carbon circular bioeconomy. Microalgal treatment of a variety of wastewaters with the conversion of generated biomass into value-added products such as bio-energy and pharmaceuticals, along with agricultural products is elaborated. Challenges encountered in large-scale implementation of microalgal technologies for low-carbon circular bioeconomy are discussed along with solutions and future perceptions. Emphasis on the suitability of microalgae in wastewater treatment and its conversion into alternate low-carbon footprint bio-energies and value-added products enforcing a zero-carbon circular bioeconomy is the major focus of this review.


Asunto(s)
Microalgas , Humanos , Aguas Residuales , Carbono , Biocombustibles , Dióxido de Carbono , Biomasa
7.
Bioresour Technol ; 358: 127381, 2022 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-35644452

RESUMEN

Bread is Europe's most wasted food, and the second most wasted food after potatoes in UK. Bread waste (BW) is a clean source of high-quality fermentable sugars. In this study, the potential of Enterobacter ludwigii to accumulate 2,3-butanediol (BDO) from BW was evaluated. Initially, the optimal inoculum size and yeast extract concentration were determined, followed by extraction of sugars from BW using acid and enzymatic hydrolysis. A glucose yield of 330-530 g/kg BW was obtained, and the sugars released were utilised for BDO production by E. ludwigii. The fed-batch cultivation using pure glucose and glucose rich hydrolysates from acid and enzymatic hydrolysis resulted in BDO titres of 144.5, 135.4, and 138.8 g/L, after 96 h, with yield of 0.47, 0.42 and 0.48 g/g yield, respectively. The innovation of the work is valorisation of BW to BDO with a circular biorefining approach and thus, reducing BW disposal and associated environmental burden.


Asunto(s)
Eliminación de Residuos , Pan , Butileno Glicoles , Fermentación , Glucosa
8.
Bioresour Technol ; 357: 127329, 2022 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-35589045

RESUMEN

Microalgae have been a promising alternative source of high-value compounds to replace the non-sustainable fossil fuels resource. The recent research development of algae-based bioproducts has remarkable impact various industries section for its renewability, efficiency, and environmentally friendly crops over those synthetic-made product. However, by utilizing microalgae biomass toward their full potential is still limited due to lack of research funding, social acceptability and challenges in policy implementation. This present review highlights the various microalgae biotechnology with consideration of economical aspect for the global potential of algae market, comparison between the microalgae market in Malaysia and international countries. In addition, the cultivation technologies and feasibility of microalgae biomass production globally, followed by insightful challenges and future development of microalgae industry are mentioned. The current study will contribute to the understanding of upstream and downstream of microalgae processing along with technical economical understandings for the successful commercialisation of microalgae products.


Asunto(s)
Microalgas , Biocombustibles , Biomasa , Biotecnología , Productos Agrícolas
9.
Bioresour Technol ; 355: 127303, 2022 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-35562022

RESUMEN

In recent years, biomass has been reported to obtain a wide range of value-added products. Biochar can be obtained by heating biomass, which aids in carbon sinks, soil amendments, resource recovery, and water retention. Microwave technology stands out among various biomass heating technologies not only for its effectiveness in biomass pyrolysis for the production of biochar and biofuel but also for its speed, volumetrics, selectivity, and efficiency. The features of microwave-assisted biomass pyrolysis and biochar are briefly reviewed in this paper. An informative comparison has been drawn between microwave-assisted pyrolysis and conventional pyrolysis. It focuses mainly on technological and economic scenario of biochar production and environmental impacts of using biochar. This source of knowledge would aid in the exploration of new possibilities and scope for employing microwave-assisted pyrolysis technology to produce biochar.


Asunto(s)
Microondas , Pirólisis , Biomasa , Carbón Orgánico
10.
Bioresour Technol ; 344(Pt B): 126264, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-34737053

RESUMEN

The complex structure of lignocellulosic biomass forms the recalcitrance to prevent the embedded holo-cellulosic sugars from undergoing the biodegradation. Therefore, a pretreatment is often required for an efficient enzymatic lignocellulosic hydrolysis. Recently, glycerol organosolv (GO) pretreatment is revealed potent in selective deconstruction of various lignocellulosic biomass and effective improvement of enzymatic hydrolysis. Evidently, the GO pretreatment is capable to modify the structure of dissolved components by glycerolysis, i.e., by trans-glycosylation onto glyceryl glycosides and by hydroxylation grafting onto glyceryl lignin. Such modifications tend to protect these main components against excessive degradation, which can be mainly responsible for the obviously less fermentation inhibitors arising in the GO pretreatment. This pretreatment can provide opportunities for valorization of emerging lignocellulosic biorefinery with production of value-added biochemicals. Recent advances in GO pretreatment of lignocellulosic biomass followed by enzymatic hydrolysis are reviewed, and perspectives are made for addressing remaining challenges.


Asunto(s)
Glicerol , Azúcares , Biomasa , Hidrólisis , Lignina
11.
Bioresour Technol ; 328: 124859, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33621759

RESUMEN

The potential of new trimetallic (Ce, Cu, La) loaded montmorillonite clay catalyst for synthesizing biodiesel using novel non-edible Celastrus paniculatus Willd seed oil via two-step transesterification reaction has been reported along with catalyst characterization. Transesterification reaction was optimized and maximum biodiesel yield of 89.42% achieved under optimal operating reaction states like; 1:12 oil to methanol ratio, 3.5% of catalyst amount, 120 °C of reaction temperature for 3 h. The predicted and experimental biodiesel yields under these reaction conditions were 89.42 and 89.40%, which showing less than 0.05% variation. Additionally, optimum biodiesel yield can be predicted by drawing 3D surface plots and 2D contour plots using MINITAB 17 software. For the characterization of the obtained biodiesel, analysis including the GC/MS, FT-IR, 1H NMR and 13C NMR were applied. The fuel properties of obtained biodiesel agrees well with the different European Union (EU-14214), China (GB/T 20828), and American (ASTM-951, 6751) standards.


Asunto(s)
Bentonita , Biocombustibles , Biocombustibles/análisis , Catálisis , China , Esterificación , Aceites de Plantas/análisis , Espectroscopía Infrarroja por Transformada de Fourier
12.
Ultrason Sonochem ; 72: 105417, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33352467

RESUMEN

Recently, chitin and chitosan are widely investigated for food preservation and active packaging applications. Chemical, as well as biological methods, are usually adopted for the production of these biopolymers. In this study, modification to a chemical method of chitin synthesis from shrimp shells has been proposed through the application of high-frequency ultrasound. The impact of sonication time on the deproteinization step of chitin and chitosan preparation was examined. The chemical identities of chitin and chitosan were verified using infrared spectroscopy. The influence of ultrasound on the deacetylation degree, molecular weight and particle size of the biopolymer products was analysed. The microscopic characteristics, crystallinity and the colour characteristics of the as-obtained biopolymers were investigated. Application of ultrasound for the production of biopolymers reduced the protein content as well as the particle size of chitin. Chitosan of high deacetylation degree and medium molecular weight was produced through ultrasound assistance. Finally, the as-derived chitosan was applied for beef preservation. High values of luminosity, chromatid and chrome were noted for the beef samples preserved using chitosan films, which were obtained by employing biopolymer subjected to sonication for 15, 25 and 40 min. Notably; these characteristics were maintained even after ten days of packaging. The molecular weight of these samples are 73.61 KDa, 86.82 KDa and 55.66 KDa, while the deacetylation degree are 80.60%, 92.86% and 94.03%, respectively; in the same order, the particle size of chitosan are 35.70 µm, 25.51 µm and 20.10 µm.


Asunto(s)
Quitina/química , Quitosano/química , Proteínas/aislamiento & purificación , Sonicación , Acetilación , Animales , Peso Molecular , Proteínas/análisis , Proteínas/química
13.
Bioresour Technol ; 305: 123072, 2020 Feb 22.
Artículo en Inglés | MEDLINE | ID: mdl-32163881

RESUMEN

This work aimed to study an newly isolated microalgal strain, Chlamydomonas sp. QWY37, that can achieve a maximum carbohydrate production of 944 mg/L·d, along with high pollutant removal efficiencies (chemical oxygen demand: 81%, total nitrogen: 96%, total phosphate: nearly 100%) by optimizing culture conditions and using an appropriate operation strategy. Through a cell-displayed technology that utilizes combined engineered system, a maximum microalgal bioethanol yield of 61 g/L was achieved. This is the first report demonstrating the highest microalgal carbohydrate productivity using swine wastewater without any pretreatments associated with direct high-density bioethanol production from the subsequent microalgal biomass. This work may represent a breakthrough in achieving feasible microalgal bioethanol conversion from real swine wastewater.

14.
Appl Microbiol Biotechnol ; 98(17): 7283-97, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24965557

RESUMEN

This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future.


Asunto(s)
Ananas/enzimología , Biotecnología/métodos , Bromelaínas/aislamiento & purificación , Bromelaínas/metabolismo
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