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1.
Biomarkers ; 20(8): 565-71, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26671823

RESUMEN

Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a secreted glycoprotein that belongs to a group of transporters of small lipophilic molecules in circulation. LCN2 has been recently characterized as an adipose-derived cytokine. This adipokine is believed to bind small substances, such as steroids and lipopolysaccharides, and has been reported to have roles in the induction of apoptosis in hematopoietic cells, transport of fatty acids and iron, modulation of inflammation, and metabolic homeostasis. Recently, LCN2 has emerged as a useful biomarker and rheumatic diseases. This review provides an overview of LCN2 in inflammation, immunity, and metabolism.


Asunto(s)
Proteínas de Fase Aguda/metabolismo , Mediadores de Inflamación/metabolismo , Inflamación/metabolismo , Lipocalinas/metabolismo , Enfermedades Metabólicas/metabolismo , Proteínas Proto-Oncogénicas/metabolismo , Animales , Biomarcadores/metabolismo , Humanos , Inflamación/diagnóstico , Lipocalina 2 , Enfermedades Metabólicas/diagnóstico , Valor Predictivo de las Pruebas , Pronóstico
2.
Sci Rep ; 5: 16674, 2015 Nov 12.
Artículo en Inglés | MEDLINE | ID: mdl-26560022

RESUMEN

Recent studies confer to IL-36α pro-inflammatory properties. However, little is known about the expression and function of IL-36α in cartilage. This study sought to analyze the expression of IL-36α in healthy and OA cartilage. Next, we determined the effects of recombinant IL-36α on catabolism and inflammation in chondrocytes. For completeness, part of the signaling pathway elicited by IL-36α was also explored. IL-36α expression was evaluated by immunohistochemistry and RT-qPCR. Expression of MMP-13, NOS2 and COX-2 was also determined in OA articular chondrocytes treated with recombinant IL-36α. IκB-α and P-p38 was explored by western blot. We observed a low constitutive expression of IL-36α in healthy human chondrocytes. However, OA chondrocytes likely expressed more IL-36α than healthy chondrocytes. In addition, immune cells infiltrated into the joint and PBMCs express higher levels of IL-36α in comparison to chondrocytes. OA chondrocytes, treated with IL-36α, showed significant increase in the expression of MMP-13, NOS2 and COX-2. Finally, IL-36α stimulated cells showed NFκB and p38 MAPK activated pathways. IL-36α acts as a pro-inflammatory cytokine at cartilage level, by increasing the expression of markers of inflammation and cartilage catabolism. Like other members of IL-1 family, IL-36α acts through the activation of NFκB and p38 MAPK pathway.


Asunto(s)
Condrocitos/metabolismo , Inflamación/genética , Inflamación/metabolismo , Interleucina-1/genética , Interleucina-1/metabolismo , Biomarcadores , Estudios de Casos y Controles , Células Cultivadas , Ciclooxigenasa 2/metabolismo , Expresión Génica , Humanos , Interleucina-1beta/metabolismo , Metaloproteinasa 13 de la Matriz/metabolismo , Modelos Biológicos , FN-kappa B/metabolismo , Óxido Nítrico Sintasa de Tipo II/metabolismo , Osteoartritis/genética , Osteoartritis/metabolismo , Transducción de Señal , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo
3.
PLoS One ; 10(8): e0135979, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26305372

RESUMEN

OBJECTIVES: Osteoarthritis (OA) is a chronic joint disease, characterized by a progressive loss of articular cartilage. During OA, proinflammatory cytokines, such as interleukin IL-1, induce the expression of matrix metalloproteinases (MMPs) in chondrocytes, contributing thus to the extracellular matrix (ECM) degradation. Members of Serpine family, including plasminogen activator inhibitors have been reported to participate in ECM regulation. The aim of this study was to assess the expression of serpin peptidase inhibitor clade E member 2 (SERPINE2), under basal conditions and in response to increasing doses of IL-1α, in human cultured chondrocytes. We also examined the effects of SERPINE2 on IL-1α-induced MMP-13 expression. For completeness, the signaling pathway involved in this process was also explored. METHODS: SERPINE2 mRNA and protein expression were evaluated by RT-qPCR and western blot analysis in human T/C-28a2 cell line and human primary chondrocytes. These cells were treated with human recombinant SERPINE2, alone or in combination with IL-1α. ERK 1/2, NFκB and AP-1 activation were assessed by western blot analysis. RESULTS: Human cultured chondrocytes express SERPINE2 in basal condition. This expression increased in response to IL-1α stimulation. In addition, recombinant SERPINE2 induced a clear inhibition of MMP-13 expression in IL-1α-stimulated chondrocytes. This inhibitory effect is likely regulated through a pathway involving ERK 1/2, NF-κB and AP-1. CONCLUSIONS: Taken together, these data demonstrate that SERPINE2 might prevent cartilage catabolism by inhibiting the expression of MMP-13, one of the most relevant collagenases, involved in cartilage breakdown in OA.


Asunto(s)
Interleucina-1alfa/biosíntesis , Metaloproteinasa 13 de la Matriz/biosíntesis , Osteoartritis/genética , Serpina E2/biosíntesis , Factor de Transcripción AP-1/biosíntesis , Cartílago Articular/crecimiento & desarrollo , Cartílago Articular/metabolismo , Condrocitos/metabolismo , Matriz Extracelular/genética , Regulación del Desarrollo de la Expresión Génica , Humanos , Interleucina-1alfa/genética , Sistema de Señalización de MAP Quinasas/genética , FN-kappa B/genética , Osteoartritis/fisiopatología , Cultivo Primario de Células , Serpina E2/genética , Factor de Transcripción AP-1/genética
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