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Aging (Albany NY) ; 12(12): 12324-12341, 2020 06 17.
Artículo en Inglés | MEDLINE | ID: mdl-32554857

RESUMEN

Ovarian aging affects female reproductive potential and is characterized by alterations in proteins, mRNAs and non-coding RNAs inside the ovarian follicle. Ovarian somatic cells and the oocyte communicate with each other secreting different molecules into the follicular fluid, by extracellular vesicles. The cargo of follicular fluid vesicles may influence female reproductive ability; accordingly, analysis of extracellular vesicle content could provide information about the quality of the female germ cell.In order to identify the most significant deregulated microRNAs in reproductive aging, we quantified the small extracellular vesicles in human follicular fluid from older and younger women and analyzed the expression of microRNAs enclosed inside the vesicles. We found twice as many small extracellular vesicles in the follicular fluid from older women and several differentially expressed microRNAs. Correlating microRNA expression profiles with vesicle number, we selected 46 deregulated microRNAs associated with aging. Bioinformatic analyses allowed us to identify six miRNAs involved in TP53 signaling pathways. Specifically, miR-16-5p, miR214-3p and miR-449a were downregulated and miR-125b, miR-155-5p and miR-372 were upregulated, influencing vesicle release, oocyte maturation and stress response. We believe that this approach allowed us to identify a battery of microRNAs strictly related to female reproductive aging.


Asunto(s)
Envejecimiento/genética , Vesículas Extracelulares/metabolismo , Líquido Folicular/citología , MicroARNs/metabolismo , Reproducción/genética , Adulto , Biología Computacional , Vesículas Extracelulares/ultraestructura , Femenino , Líquido Folicular/metabolismo , Perfilación de la Expresión Génica , Humanos , Infertilidad Masculina/terapia , Masculino , Microscopía Electrónica de Rastreo , Folículo Ovárico/metabolismo , Inyecciones de Esperma Intracitoplasmáticas , Tetraspanina 28/genética , Tetraspanina 28/metabolismo , Regulación hacia Arriba
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