RESUMEN
OBJECTIVE: To investigate the lipid peroxidation (PEROX) processes in primary open-angle glaucoma (POAG) patients, and whether this mechanism may be related to disease progression. MATERIAL AND METHODS: A prospective, observational, cross-sectional, non-experimental, and analytical study was conducted on a case and a comparison group, consisting of 175 surgical patients divided into: 1) POAG group (GG; n=88) and 2) comparison group of patients with cataracts (CG; n=87). Demographic data, patient characteristics, lifestyle data, as well as ophthalmological examination were registered in an Excel spreadsheet. Biochemical data were obtained by processing the aqueous humor collected at the beginning of surgery. Determination of malondialdehyde/thiobarbituric acid reactive substances (MDA/TBARS) and total antioxidant activity (AAO) was assayed using enzymatic-colorimetric methods in the aqueous humor samples. Statistical analysis was performed using SPSS 15.0 software. RESULTS: Aqueous humor MDA/TBARS levels were significantly higher (P<.001) and the AAO significantly lower (P<.001) in the GG than in the GC. The MDA/TBARS directly correlated with intraocular pressure (IOP) values and the cup-to-disc ratio (CDR). Decreased AAO activity correlated inversely with IOP and CDR. Differences between groups were noticeably higher in the GG as regards obesity, alcohol consumption, anxiety, depression, and sedentary lifestyle. In the multivariate analysis, the variables that showed a better predictive ability were: MDA/TBARS, PIO, AAO, CDR, and depression. CONCLUSIONS: The POAG patients have a PEROX background that is reflected in the aqueous humor by variations in MDA/TBARS and AAO. Moreover, both the MDA/TBARS and AAO correlated with IOP values and the CDR. We propose that determination of MDA/TBARS and AAO in the aqueous humor of POAG patients can be used as biomarkers for monitoring the disease, as well the changes in lifestyle and other related risk factors.
Asunto(s)
Antioxidantes/análisis , Humor Acuoso/química , Glaucoma de Ángulo Abierto/metabolismo , Peroxidación de Lípido , Sustancias Reactivas al Ácido Tiobarbitúrico/análisis , Adulto , Anciano , Anciano de 80 o más Años , Catarata/metabolismo , Colorimetría , Estudios Transversales , Femenino , Humanos , Masculino , Malondialdehído/análisis , Persona de Mediana Edad , Estudios ProspectivosRESUMEN
CASE REPORT: A 50-year-old man presented with a giant and invasive mass at the nasal limbus in his right eye. A surgical excision without safety margins was promptly performed, followed by interferon alpha-2b (IFNα2b) eye drops. After monitoring the patient for two years and after performing a number of biopsies, he remains free from any signs of disease. DISCUSSION: For cases of giant and invasive squamous cell carcinoma, to reverse the order of treatment, by first performing an early tumour excision, followed by the application of topical IFNα2b, may prove useful to achieve total remission of the tumour.