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Objective:To construct a recombinant plasmid pET30a-leucine-rich repeat (LRR) containing 15 (LRRC15) of Taenia solium, prokaryotically express and purify the LRRC15 recombinant protein, and prepare a rabbit polyclonal antibody. Methods:The LRRC15 protein encoding gene of Taenia solium was obtained by whole gene synthesis; it was cloned into pET30a vector, and the recombinant plasmid pET30a-LRRC15 was constructed and identified by double-enzyme PCR; the recombinant plasmid was transformed into competent cells of Escherichia coli BL21 (DE3), and the recombinant protein LRRC15 was induced to express by isopropyl-beta-D-thiogalactopyranoside (IPTG), the expression product was analyzed and identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); the LRRC15 recombinant protein was purified by Ni-IDA affinity columns, the purified recombinant protein was analyzed and identified by SDS-PAGE, and the specificity of the purified recombinant protein was identified by Western blot (WB); the New Zealand rabbits were immunized with purified LRRC15 recombinant protein to prepare polyclonal antibodies against LRRC15, and the potency of the purified polyclonal antibody was determined by indirect enzyme-linked immunosorbent assay (ELISA). Results:After PCR identification, a band with a length of 1 506 bp was amplified, which was consistent with the LRRC15 gene; after SDS-PAGE and WB identification, the LRRC15 target protein with a relative molecular mass ( Mr) of about 55.36 × 10 3 was obtained; after immunizing New Zealand rabbits with purified LRRC15 recombinant protein, a polyclonal antibody against LRRC15 was obtained, and its potency was 1∶1 587 200. Conclusion:The recombinant plasmid pET30a-LRRC15 is successfully constructed, the LRRC15 recombinant protein of Taenia solium is prepared, and a high purity and high potency rabbit anti polyclonal antibody against LRRC15 recombinant protein is obtained.
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Taeniasis is a kind of zoonotic parasitosis which seriously endangers human health. Drug therapy and vaccine control is one of the hotspots in current research. At present, proteomics has become an important tool for developing vaccines and understanding the pathogenic mechanism. The differences in protein patterns between different developmental stages of parasites may reflect the specific strategies and adaptive mechanisms used in the constantly changing environment and life cycle. Therefore, this article reviews the proteomics research progress of various tapeworms, aiming at providing a reference for prevention and control of Taeniasis.
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Objective To investigate risk factors and characteristics of the pathogens for hospital-acquired pneumonia(HAP) in elderly psychiatric patients Methods Data were collected from 259 psychiatric patients aged over 60 hospitalized between January 2012 and December 2014,and risk factors for HAP and characteristics of the pathogens were analyzed.Results The incidence of HAP in the elderly psychiatric patients was 40.4％.Main risk factors for HAP included long-term bed rest,dysphagia,specific types of mental illness,underlying comorbidities,length of hospitalization,psychiatric medications,and age.A total of 137 pathogenic microbialstrains were isolated,of which,Gram-negative species accounted for 55.47％,with 32.12 ％ as Gram-positive species and 12.41 ％ as fungal species.Conclusions The main risk factors for HAP in elderly psychiatric patients are long-term bed rest,dysphagia,specific types of mental illness,underlying comorbidities,length of hospitalization,psychiatric medications,and age.The most common pathogens are Gram-negative microorganisms,followed by Gram-positive microorganisms and fungi.It is very important to take proactive measures to prevent the occurrence of HAP and improve prognosis.
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Objective: To observe the effectiveness of combinative use of gentamycin, metronidazole and rifampin in the treatment of ulcerative colonitis and explore the etiology of the disease. Methods: 30 and 26 patients were allocated to treatment group and control group, respectively. Maintained clysis with a combination of gentamycin, metronidazole and rifampin in warm normal saline were administered to those in the treatment group twice a day for two weeks, while SASP and prednisone were administered to those in the control group for two weeks. Results: The alleviation rate of the treatment group is higher than that of the control group. Conclusion: Combinative use of gentamycin, metronidazole and rifampin for maintained clysis is a effective treatment for ulcerative colonitis.