RESUMEN

The guanosine tri-phosphatase Ran stimulates assembly of microtubule spindles. However, it is not known what aspects of the microtubule cytoskeleton are subject to regulation by Ran in mitosis. Here we show that Ran-GTP stimulates microtubule assembly by increasing the rescue frequency of microtubules three- to eightfold. In addition to changing microtubule dynamics, Ran-GTP also alters the balance of motor activities, partly as a result of an increase in the amount of motile Eg5, a plus-end-directed microtubule motor that is essential for spindle formation. Thus, Ran regulates multiple processes that are involved in spindle assembly.

Asunto(s)

Microtúbulos/metabolismo , Proteínas Motoras Moleculares/metabolismo , Huso Acromático/fisiología , Proteínas de Xenopus , Proteína de Unión al GTP ran/metabolismo , Animales , Cromatina/metabolismo , Dimetilsulfóxido/farmacología , Cinesinas/metabolismo , Oocitos/química , Oocitos/metabolismo , Paclitaxel/farmacología , Proteínas Proto-Oncogénicas c-mos/farmacología , Huso Acromático/efectos de los fármacos , Xenopus laevis

Asunto(s)

Microtúbulos/fisiología , Tubulina (Proteína)/fisiología , Animales , Humanos , Microtúbulos/ultraestructura