RESUMEN

The in vitro production of platelets could provide a life-saving intervention for patients that would otherwise require donor-derived platelets. Producing large numbers of platelets in vitro from their progenitor cells, megakaryocytes, remains remarkably difficult and inefficient. Here, a human megakaryoblast leukemia cell line (MEG-01) was used to assess the maturation of megakaryocytes and to develop a new methodology for producing high numbers of platelet-like particles from mature MEG-01 cells in vitro.

Asunto(s)

Plaquetas/patología , Micropartículas Derivadas de Células/patología , Ensayos Analíticos de Alto Rendimiento , Leucemia Megacarioblástica Aguda/patología , Megacariocitos/patología , Trombopoyesis , Plaquetas/metabolismo , Técnicas de Cultivo de Célula , Línea Celular Tumoral , Micropartículas Derivadas de Células/metabolismo , Técnica del Anticuerpo Fluorescente , Humanos , Leucemia Megacarioblástica Aguda/metabolismo , Megacariocitos/metabolismo , Microscopía Fluorescente