RESUMEN
Vascular endothelial dysfunction and inflammatory response are early events during initiation and progression of atherosclerosis. In vitro studies have described that CIT markedly upregulates expressions of ICAM-1 and VCAM-1 of endothelial cells, which result from NF-κB activation induced by CIT. In order to determine whether it plays a role in atherogenesis in vivo, we conducted the study to investigate the effects of CIT on atherosclerotic plaque development and inflammatory response in apolipoprotein E deficient (apoE-/-) mice. Five-week-old apoE-/- mice were fed high-fat diets and treated with CIT for 15 weeks, followed by assay of atherosclerotic lesions. Nitric oxide (NO), vascular endothelial growth factor (VEGF) and endothelin-1 (ET-1) were detected in serum. Levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), VEGF, and ET-1 in plaque areas of artery walls were examined. NF-κB p65 expression and NF-κB activation in aorta also were assessed. CIT treatment significantly augmented atherosclerotic plaques and increased expressions of ICAM-1, VCAM-1, VEGF and ET-1 in aorta. Mechanistic studies showed that activation of NF-κB was significantly elevated by CIT treatment, indicating the effect of CIT on atherosclerosis may be regulated by activation of NF-κB.
Asunto(s)
Aterosclerosis/complicaciones , Aurovertinas/toxicidad , Endotelio Vascular/fisiopatología , Hipercolesterolemia/complicaciones , Inflamación/complicaciones , Regulación hacia Arriba/efectos de los fármacos , Animales , Aorta/efectos de los fármacos , Aorta/patología , Apolipoproteínas E/deficiencia , Aterosclerosis/sangre , Peso Corporal/efectos de los fármacos , Dieta Alta en Grasa , Endotelina-1/metabolismo , Endotelio Vascular/efectos de los fármacos , Endotelio Vascular/patología , Hipercolesterolemia/sangre , Molécula 1 de Adhesión Intercelular/metabolismo , Lípidos/sangre , Masculino , Ratones Endogámicos C57BL , FN-kappa B/metabolismo , Óxido Nítrico/metabolismo , Molécula 1 de Adhesión Celular Vascular/metabolismo , Factor A de Crecimiento Endotelial Vascular/metabolismoRESUMEN
Environmental magnetic measurements were carried out on the samples of indoor dustfall collected from Qiyun building at different heights in Lanzhou University. The results indicate a high concentration of magnetic minerals in indoor dustfall, dominated by pseudo-single domain (PSD) and multiple domain (MD) ferrimagnetic minerals which are mainly from air pollution. The concentration and granulometry of magnetic minerals decrease with the increase of the height within 24 m near surface. We call it near surface blowing model dominated by low pollution sources. Within 10 m near the top of the building, the concentration and granulometry of magnetic mineral show the synchronous change with the increase of height and that is called building damping model dominated by high pollution sources. The concentration of magnetic minerals can reflect particulate matter pollution in Lanzhou. The results show the pollution is relatively slight from 20-30 m and can provide a scientific basis for atmospheric dust management and improvement at the same time.