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PURPOSE: Our study aimed to develop a relatively accurate gastric cancer (GC) screening score system for urban residents and to validate the screening efficacy. METHODS: The present study included a derivation cohort (n = 3406) and a validation cohort (n = 868) of urban residents. Applying the full-stack engineering intelligent system platform of Hualian Health Big Data of Shandong University, the clinical physical examination data of subjects were collected. Univariate and multivariate analyses were used to identify risk factors for GC, and subsequently, an optimal prediction rule was established to create three distinct scoring systems. RESULTS: In the GC-risk scoring system I, age, plateletocrit (PCT), carcinoembryonic antigen (CEA), glucose, albumin, creatinine were independent risk factors of GC, with scores ranging from 0 to 28 and optimal cut-off was 15.5. The second scoring system consisted of age, PCT, RDW-CV, CEA, glucose, albumin, and creatinine, with scores ranging from 0 to 31. The optimal cut-off point was determined to be 15.5. The scoring system III comprise of age, sex, PCT, RDW CV, CEA, glucose, with scores ranging from 0 to 21 and optimal cut-off was 10.5. All three scoring systems demonstrated excellent discrimination for GC, achieving an AUC of 0.884, 0.89, and 0.876, respectively. In external validation, the AUC values were 0.654, 0.658, and 0.714. Notably, the GC-risk scoring system III exhibited the highest screening efficiency. CONCLUSIONS: Urban residents benefited from the effective and verified GC-risk scoring systems, which demonstrated excellent performance in identifying individuals with an elevated risk of GC.
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INTRODUCTION AND OBJECTIVES: The relationship between anemia and the outcome of patients with cirrhosis is not completely clear. Therefore, we performed this large-scale epidemiological study to investigate the prevalence and severity of anemia in patients with cirrhosis and acute decompensation or liver injury and how anemia impacts short-term and long-term outcomes. PATIENTS AND METHODS: Patients with cirrhosis and acute decompensation (AD) or acute liver injury (ALI) were enrolled in the Chinese AcuTe on CHronic LIver FailurE (CATCH-LIFE) studies, which consisted of two large, multicenter, prospective, observational cohorts between January 2015 and December 2016 and July 2018 and January 2019. We conducted data analysis on the prevalence of anemia and determined the relationship between anemia and prognosis. RESULTS: Among 1979 patients, 1389 (70.2%) had anemia, among whom 599 (41.3%) had mild anemia, 595 (15.8%) had moderate anemia and 195 (2.4%) had severe anemia. A linear association between hemoglobin level and 90-day or 1-year LT-free mortality was shown, and a 10 g/L decrease in hemoglobin level was associated with a 6.8% extra risk of 90-day death and a 5.7% extra risk of 1-year death. Severe anemia was an independent risk factor for 90-day [HR=1.649 (1.100, 2.473), p=0.016] and 1-year LT-free mortality [HR=1.610 (1.159, 2.238), p=0.005]. Multinomial logistic regression analysis further identified that severe anemia was significantly associated with post-28-day mortality but not within-28-day mortality. CONCLUSIONS: Anemia is common in patients with cirrhosis admitted for acute events. Severe anemia was associated with poor 90-day and 1-year prognoses in these patients.
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OBJECTIVE: This study aimed to perform a meta-analysis to investigate the diagnostic safety and accuracy of Ultrasound-Guided Core Needle Biopsy (US-CNB) Axillary Lymph Nodes (ALNs) region in patients with Breast Cancer (BC). METHODS: The authors searched the electronic databases PubMed, Scopus, Embase, and Web of Science for clinical trials about US-CNB for the detection of ALNs in breast cancer patients. The authors extracted and pooled raw data from the included studies and performed statistical analyses using Meta-DiSc 1.4 and Review Manager 5.3 software. A random effects model was used to calculate the data. At the same time, data from the Ultrasound-guided Fine-Needle Aspiration (US-FNA) were introduced for comparison with the US-CNB. In addition, the subgroup was performed to explore the causes of heterogeneity. (PROSPERO ID: CRD42022369491). RESULTS: In total, 18 articles with 2521 patients were assessed as meeting the study criteria. The overall sensitivity was 0.90 (95% CI [Confidence Interval], 0.87â0.91; p = 0.00), the overall specificity was 0.99 (95% CI 0.98â1.00; p = 0.62), the overall area under the curve (AUC) was 0.98. Next, in the comparison of US-CNB and US-FNA, US-CNB is better than US-FNA in the diagnosis of ALNs metastases. The sensitivity was 0.88 (95% CI 0.84â0.91; p = 0.12) vs. 0.73 (95% CI 0.69â0.76; p = 0.91), the specificity was 1.00 (95% CI 0.99â1.00; p = 1.00) vs. 0.99 (95% CI 0.67â0.74; p = 0.92), and the AUC was 0.99 vs. 0.98. Subgroup analysis showed that heterogeneity may be related to preoperative Neoadjuvant Chemotherapy (NAC) treatment, region, size of tumor diameter, and the number of punctures. CONCLUSION: US-CNB has a satisfactory diagnostic performance with good specificity and sensitivity in the preoperative diagnosis of ALNs in BC patients.
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Neoplasias de la Mama , Humanos , Femenino , Metástasis Linfática/patología , Neoplasias de la Mama/patología , Biopsia con Aguja Gruesa , Axila/patología , Sensibilidad y Especificidad , Ganglios Linfáticos/diagnóstico por imagen , Ganglios Linfáticos/patología , Ganglios Linfáticos/cirugía , Ultrasonografía Intervencional , Estudios RetrospectivosRESUMEN
ABSTRACT Introduction: Kungfu is a traditional Chinese sports activity, and many optional courses are offered in many universities. However, during kung fu practice, sports injuries can be caused. Objective: Investigate the most common types of sports injuries caused by kung fu practice and present intervention countermeasures. Methods: A total of 612 college students practicing kung fu at 5 universities were investigated, and a 12-week follow-up survey was conducted to collect data on the types, causes, and injury grades of their sports injuries. According to the survey results, reasonable prevention and intervention measures were formulated to ensure the safety of kung fu practice. Results: The sports injuries caused by kung fu practice mainly occurred at the waist, knees, and ankles, and the types of sports injuries were mainly by ligament strain, which was mainly caused by improper preparation, poor physical functioning, and mental state, incorrect technical movements, etc. Conclusion: Kung fu practice can improve the physical health of college students, but it can also lead to sports injuries, especially to the pelvic girdle, knees, and ankles. Strict preventive measures should be taken to prevent sports injuries from affecting the physical health of college students. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: O kung fu é uma atividade esportiva tradicional chinesa, sendo que muitos cursos opcionais são oferecidos em várias universidades. Porém, durante a prática de kung fu, lesões esportivas podem ser causadas. Objetivo: Investigar os tipos mais comuns de lesões esportivas causadas pela prática de kung fu e apresentar as contramedidas de intervenção. Métodos: Um total de 612 estudantes universitários praticantes de kung fu em 5 universidades foram investigados, uma pesquisa de acompanhamento de 12 semanas foi realizada para coletar dados sobre os tipos, causas e graus de lesão de suas lesões esportivas. De acordo com os resultados da pesquisa, foram formuladas medidas razoáveis de prevenção e intervenção para garantir a segurança da prática de kung fu. Resultados: As lesões esportivas causadas pela prática de kung fu ocorreram principalmente na cintura, joelhos e tornozelos, e os tipos de lesões esportivas foram principalmente por tensão ligamentar, que foi causada principalmente por preparação inadequada, mau funcionamento físico e estado mental, movimentos técnicos incorretos, etc. Conclusão: A prática de kung fu pode melhorar a saúde física dos estudantes universitários, mas também pode levar a lesões esportivas, principalmente na cintura pélvica, joelhos e tornozelos. Medidas preventivas rigorosas devem ser tomadas para evitar que lesões esportivas afetem a saúde física dos estudantes universitários. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: El kung fu es una actividad deportiva tradicional china, y en muchas universidades se ofrecen cursos opcionales. Sin embargo, durante la práctica del kung fu pueden producirse lesiones deportivas. Objetivo: Investigar los tipos más comunes de lesiones deportivas causadas por la práctica del kung fu y presentar las contramedidas de intervención. Métodos: Se investigó a un total de 612 estudiantes universitarios que practicaban kung fu en 5 universidades, y se realizó una encuesta de seguimiento de 12 semanas para recoger datos sobre los tipos, las causas y los grados de lesión de sus lesiones deportivas. Según los resultados de la investigación, se formularon medidas razonables de prevención e intervención para garantizar la seguridad de la práctica del kung fu. Resultados: Las lesiones deportivas causadas por la práctica del kung fu se produjeron principalmente en la cintura, las rodillas y los tobillos, y los tipos de lesiones deportivas fueron sobre todo por distensión de los ligamentos, lo que fue causado principalmente por una preparación inadecuada, un mal funcionamiento físico y un estado mental, movimientos técnicos incorrectos, etc. Conclusión: La práctica del kung fu puede mejorar la salud física de los estudiantes universitarios, pero también puede provocar lesiones deportivas, principalmente en la cintura pélvica, las rodillas y los tobillos. Deben adoptarse estrictas medidas preventivas para evitar que las lesiones deportivas afecten a la salud física de los estudiantes universitarios. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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ABSTRACT Introduction: Ballroom dancing has become popular as an exercise method that encompasses many benefits in the social aspect. However, some middle-aged and elderly dancers have suffered from injuries and other problems from dancing. The comprehensive understanding of gymnastic training to solve this problem has not yet been raised, without which no adequate means of prevention can be outlined. Objective: Study the effect of balanced aerobic training on the physical fitness of middle-aged and elderly ballroom dance practitioners. Methods: A study was conducted with 50 volunteers practicing ballroom dancing, middle-aged and elderly, divided into experimental and control groups. According to the community square dance protocol, the control group performed the dance for one hour daily. The experimental group also used the same period and technique, introducing balanced aerobic exercises. Fitness and performance data were collected before and after the intervention, analyzed, and presented statistically. Results: In the experimental group, the growth rate of the grip strength index was 28.70%, the lumbar strength index was 32.27%, the time rate in the orthostatic position was 6.28%, and the sitting forward flexion index was 6.69%. Conclusion: Compared to traditional community sports, balanced aerobics inserted into ballroom dance training showed an improvement effect on the physical and sports fitness of middle-aged and elderly women, and may also prevent the adverse effects of aging on activities of daily living, and its implementation is valid. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: A dança de salão popularizou-se como um método de exercício físico que abrange muitos benefícios no aspecto social. Contudo, alguns dançarinos de meia idade e idosos têm sofrido com lesões e outros problemas decorrentes da dança. A compreensão abrangente do treino com ginástica para a solução desse problema ainda não foi levantada, sem a qual não se pode traçar meios adequados de prevenção. Objetivo: Estudar o efeito do treinamento aeróbico balanceado sobre a aptidão física de praticantes de dança de salão de meia-idade e idosos. Métodos: Foi elaborado um estudo com 50 voluntários praticantes de dança de salão, de meia idade e idosos, divididos em grupos experimental e controle. O grupo de controle realizou a dança durante uma hora diária, de acordo com o protocolo de dança da praça comunitária. O grupo experimental também utilizou o mesmo período e técnica, introduzindo exercícios aeróbicos balanceados. Os dados de aptidão física e desempenho foram coletados antes e após a intervenção, analisados e apresentados estatisticamente. Resultados: No grupo experimental, a taxa de crescimento do índice de força de preensão foi de 28,70%, o índice de força lombar foi de 32,27%, a taxa de tempo em posição ortostática foi de 6,28%, e o índice de flexão sentado para frente foi de 6,69%. Conclusão: Em comparação com os esportes comunitários tradicionais, a aeróbica equilibrada inserida no treino de dança de salão apresentou um efeito de melhoria na aptidão física e esportiva das mulheres de meia-idade e idosas, podendo prevenir também os efeitos adversos do envelhecimento sobre as atividades de vida diária, sendo válida a sua implementação. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: Los bailes de salón se han popularizado como método de ejercicio físico que encierra muchos beneficios en el aspecto social. Sin embargo, algunos bailarines de mediana y avanzada edad han sufrido lesiones y otros problemas derivados del baile. Aún no se ha planteado un conocimiento exhaustivo del entrenamiento gimnástico para resolver este problema, sin el cual no se pueden esbozar medios adecuados de prevención. Objetivo: Estudiar el efecto del entrenamiento aeróbico equilibrado en la forma física de los practicantes de bailes de salón de mediana y avanzada edad. Métodos: Se desarrolló un estudio con 50 voluntarios practicantes de bailes de salón, de mediana y avanzada edad, divididos en grupos experimental y control. El grupo de control realizó el baile durante una hora diaria, según el protocolo de baile de salón de la comunidad. El grupo experimental utilizó el mismo período y la misma técnica, introduciendo ejercicios aeróbicos equilibrados. Se recogieron datos sobre la forma física y el rendimiento antes y después de la intervención, y se analizaron y presentaron estadísticamente. Resultados: En el grupo experimental, el índice de crecimiento del índice de fuerza de prensión fue del 28,70%, el índice de fuerza lumbar fue del 32,27%, el índice de tiempo en posición ortostática fue del 6,28% y el índice de flexión hacia delante sentado fue del 6,69%. Conclusión: En comparación con los deportes comunitarios tradicionales, los ejercicios aeróbicos equilibrados insertados en el entrenamiento de bailes de salón mostraron un efecto de mejora en la forma física y deportiva de las mujeres de mediana edad y ancianas, y también pueden prevenir los efectos adversos del envejecimiento en las actividades de la vida diaria, y su aplicación es válida. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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Abstract Objective: This study aimed to perform a meta-analysis to investigate the diagnostic safety and accuracy of Ultrasound-Guided Core Needle Biopsy (US-CNB) Axillary Lymph Nodes (ALNs) region in patients with Breast Cancer (BC). Methods: The authors searched the electronic databases PubMed, Scopus, Embase, and Web of Science for clinical trials about US-CNB for the detection of ALNs in breast cancer patients. The authors extracted and pooled raw data from the included studies and performed statistical analyses using Meta-DiSc 1.4 and Review Manager 5.3 software. A random effects model was used to calculate the data. At the same time, data from the Ultrasound-guided Fine-Needle Aspiration (US-FNA) were introduced for comparison with the US-CNB. In addition, the subgroup was performed to explore the causes of heterogeneity. (PROSPERO ID: CRD42022369491). Results: In total, 18 articles with 2521 patients were assessed as meeting the study criteria. The overall sensitivity was 0.90 (95% CI [Confidence Interval], 0.87‒0.91; p = 0.00), the overall specificity was 0.99 (95% CI 0.98‒ 1.00; p = 0.62), the overall area under the curve (AUC) was 0.98. Next, in the comparison of US-CNB and US-FNA, US-CNB is better than US-FNA in the diagnosis of ALNs metastases. The sensitivity was 0.88 (95% CI 0.84‒ 0.91; p = 0.12) vs. 0.73 (95% CI 0.69‒0.76; p = 0.91), the specificity was 1.00 (95% CI 0.99‒1.00; p = 1.00) vs. 0.99 (95% CI 0.67‒0.74; p = 0.92), and the AUC was 0.99 vs. 0.98. Subgroup analysis showed that heterogeneity may be related to preoperative Neoadjuvant Chemotherapy (NAC) treatment, region, size of tumor diameter, and the number of punctures. Conclusion: US-CNB has a satisfactory diagnostic performance with good specificity and sensitivity in the preoperative diagnosis of ALNs in BC patients.
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ABSTRACT Introduction: Exercise is an important part of osteoporosis treatment. A moderate amount of exercise can improve bone metabolism, improve bone biomechanics and increase bone density. Objective: To study the effects of different sports on adult bone mineral density. Methods: We selected volunteers to perform different exercises and used quantitative ultrasound and bioelectrical impedance methods to test the volunteers. Results: Exercise can increase the content of lean body mass in body composition and reduce body fat percentage. The calcaneal bone mineral density of physical education students is positively correlated with low body weight. Conclusion: Exercise can promote the bone mineral density of young adults. Stronger weight-bearing exercises can better promote the increase of bone density. Level of evidence II; Therapeutic studies - investigation of treatment results.
RESUMO Introdução: Atividades físicas são uma parte importante do tratamento de osteoporose. Uma quantidade moderada de exercícios pode melhorar a biomecânica, o metabolismo, e a densidade ósseos. Objetivo: Estudar os efeitos de diferentes esportes na densidade mineral óssea de adultos. Métodos: Selecionou-se voluntários para realizarem diferentes atividades físicas e utilizou-se ultrassom quantitativo e bioimpedância elétrica como métodos para testar os voluntários. Resultados: A atividade física pode aumentar o conteúdo de massa magra na composição corporal e reduzir a porcentagem de gordura no corpo. A densidade mineral do calcâneo de estudantes de educação física mostrou uma relação positiva com peso baixo. Conclusão: A atividade física melhorou a densidade mineral óssea de jovens. Exercícios mais intensos de suporte de carga podem promover o aumento da densidade óssea. Nível de evidência II; Estudos terapêuticos - investigação de resultados de tratamento.
RESUMEN Introducción: Actividades físicas son una parte importante del tratamiento de osteoporosis. Una cantidad moderada de ejercicios puede mejorar la biomecánica, el metabolismo y la densidad óseos. Objetivo: Estudiar los efectos de diferentes deportes en la densidad mineral ósea de adultos. Métodos: Se seleccionaron voluntarios para realizar diferentes actividades físicas y se utilizó el ultrasonido cuantitativo y la bioimpedancia eléctrica como métodos para testear a los voluntarios. Resultados: La actividad física puede aumentar el contenido de masa muscular en la composición corporal y reducir el porcentaje de grasa en el cuerpo. La densidad mineral del calcáneo de estudiantes de educación física mostró una relación positiva con peso bajo. Conclusión: La actividad física mejoró la densidad mineral ósea de jóvenes. Ejercicios más intensos de soporte de peso pueden favorecer el aumento de la densidad ósea. Nivel de evidencia II; Estudios terapéuticos - investigación de resultados de tratamiento.
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To reduce the negative impact of nanopesticide carriers of on the environment, a greener nanodelivery system is necessary. Nanogels are nontoxic and degradable carriers, however, the potential of nanogels for delivering pesticides has not been proven. In this study, poly(vinyl alcohol)-valine, an ecofriendly polymer, was synthesized and used to fabricate emamectin benzoate nanogel suspension (EB NS). The nanoformulation showed favorable stability at low temperature, high temperature or one year storage, and in water with different hardnesses. The retention of the EB NS solution on leaves was higher than that of an EB emulsifiable concentrate (EC) by approximately 9% at a concentration of 10 mg L-1. The half-life of EB nanogels under Ultra Violet irradiation was prolonged by 3.3-fold. Moreover, the bioactivity of the EB NS against Plutella xylostella was higher than that of the EB EC. These advantages resulted in a relatively long duration of pest control. The response of nanogels to laccase, a digestive enzyme in the digestive tract of lepidopteran pests, enables pesticide release on demand. Nanogels have the advantages of being ecofriendly carriers, exhibiting higher utilization, and prolonged pest control periods, and they have a brilliant future in pesticide delivery.
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Insecticidas , Alcohol Polivinílico , Ivermectina/análogos & derivados , Lignina/análogos & derivados , Nanogeles , Polietilenglicoles , Polietileneimina , ValinaRESUMEN
This study aimed to explore the relationship between plasma interleukin 6 (IL-6) levels, adverse cardiovascular events, and the severity of acute coronary syndrome (ACS). A literature review was performed of studies regarding IL-6 and ACS extracted from databases including EMBASE, Cqvip, MEDLINE, Web of Knowledge, PubMed, Cochrane Library, China National Knowledge Infrastructure, and Wanfang data. The Newcastle-Ottawa scale (NOS) was used to evaluate the quality of the literature. The literature was screened, its quality was evaluated, and relevant data were extracted for performing meta-analysis using RevMan software (version 5.3). A total of 524 studies were included in the initial survey. After several rounds of screening and analysis, six studies met the inclusion criteria and underwent meta-analysis using a fixed-effect model. Patients were divided into non-severe and severe groups based on the concentration of high-sensitivity C-reactive protein. Meta-analysis of the relationship between IL-6 and the severity of ACS showed that the plasma IL-6 level of patients in the severe group was significantly higher than that of patients in the non-severe group (p<0.00001). Additionally, patients with experience of major adverse cardiovascular events had significantly higher plasma IL-6 levels than did patients without experience of such events (p<0.00001). In summary, patients with ACS and high IL-6 levels tended to be in a critical condition, with a higher risk of adverse cardiovascular events and worse prognosis. Thus, IL-6 levels could indicate whether patients with ACS may have adverse cardiovascular events and determine the severity of ACS.
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Síndrome Coronario Agudo , Interleucina-6 , Proteína C-Reactiva , China , Humanos , PronósticoRESUMEN
Sevoflurane (SEVO) is widely applied as an anesthetic, which exerts antitumor capacity in various cancers, including hepatocellular carcinoma (HCC). Previous studies indicated that long non-coding RNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) was upregulated, while microRNA-29a-3p (miR-29a-3p) was downregulated in HCC. Thus, we aimed to explore the roles of KCNQ1OT1 and miR-29a-3p in HCC cells exposed to SEVO. Cell proliferation, apoptosis, migration, and invasion were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, and transwell assays, respectively. The levels of genes were determined by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Furthermore, the interaction between miR-29a-3p and KCNQ1OT1 or chromebox protein homolog 3 (CBX3) was predicted by Starbase or Targetscan, and then confirmed by dual-luciferase reporter assay. We found that the levels of KCNQ1OT1 and CBX3 were decreased, while miR-29a-3p was increased in SEVO-treated HCC cells. KCNQ1OT1 overexpression weakened the inhibitory effects of SEVO on HCC cell proliferation, apoptosis, migration, and invasion. Interestingly, KCNQ1OT1 bound to miR-29a-3p, and miR-29a-3p targeted CBX3. KCNQ1OT1 upregulated CBX3 level by repressing miR-29a-3p expression. Furthermore, KCNQ1OT1 exerted tumor promotion in HCC cells via suppressing miR-29a-3p to regulate CBX3 expression. Collectively, our findings demonstrated that KCNQ1OT1 regulated the antitumor effects of SEVO on HCC cells through modulating the miR-29a-3p/CBX3 axis, providing a theoretical basis for the treatment of HCC.
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Carcinoma Hepatocelular , Neoplasias Hepáticas , MicroARNs , Canales de Potasio con Entrada de Voltaje , ARN Largo no Codificante , Carcinoma Hepatocelular/tratamiento farmacológico , Carcinoma Hepatocelular/genética , Proteínas Cromosómicas no Histona , Humanos , Neoplasias Hepáticas/tratamiento farmacológico , Neoplasias Hepáticas/genética , MicroARNs/genética , ARN Largo no Codificante/genética , Sevoflurano/farmacologíaRESUMEN
PURPOSE: Glutamine plays an important role in tumor metabolism and progression. This research aimed to find out how Gln exert their effects on laryngeal squamous cell carcinoma (LSCC). METHODS: Cell proliferation was measured by CCK8 and EdU assay, mitochondrial bioenergetic activity was measured by mitochondrial stress tests. Gene expression profiling was revealed by RNA sequencing and validated by RT-qPCR. In LSCC patients, protein expression in tumor and adjacent tissues was examined and scored by IHC staining. RNAi was performed by stably expressed shRNA in TU177 cells. In vivo tumor growth analysis was performed using a nude mouse tumorigenicity model. RESULTS: Gln deprivation suppressed TU177 cell proliferation, which was restored by αKG supplementation. By transcriptomic analysis, we identified CECR2, which encodes a histone acetyl-lysine reader, as the downstream target gene for Gln and αKG. In LSCC patients, the expression of CECR2 in tumors was lower than adjacent tissues. Furthermore, deficiency of CECR2 promoted tumor cell growth both in vitro and in vivo, suggesting it has tumor suppressor effects. Besides, cell proliferation inhibited by Gln withdrawal could be restored by CECR2 depletion, and the proliferation boosted by αKG supplementation could be magnified either, suggested that CECR2 feedback suppressed Gln and αKG's effect on tumor growth. Transcriptomic profiling revealed CECR2 regulated the expression of a series of genes involved in tumor progression. CONCLUSION: We confirmed the Gln-αKG-CECR2 axis contributes to tumor growth in LSCC. This finding provided a potential therapeutic opportunity for the use of associated metabolites as a potential treatment for LSCC.
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Genes Supresores de Tumor , Glutamina/metabolismo , Neoplasias Laríngeas/genética , Carcinoma de Células Escamosas de Cabeza y Cuello/genética , Factores de Transcripción/genética , Anciano , Anciano de 80 o más Años , Animales , Recuento de Células , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Proliferación Celular/genética , Progresión de la Enfermedad , Regulación hacia Abajo , Femenino , Perfilación de la Expresión Génica , Regulación Neoplásica de la Expresión Génica , Glutamina/farmacología , Humanos , Ácidos Cetoglutáricos/metabolismo , Ácidos Cetoglutáricos/farmacología , Neoplasias Laríngeas/metabolismo , Neoplasias Laríngeas/patología , Masculino , Ratones , Ratones Desnudos , Persona de Mediana Edad , Mitocondrias/metabolismo , Proteínas de Neoplasias/metabolismo , Consumo de Oxígeno , Carcinoma de Células Escamosas de Cabeza y Cuello/metabolismo , Carcinoma de Células Escamosas de Cabeza y Cuello/patología , Factores de Transcripción/deficiencia , Factores de Transcripción/metabolismoRESUMEN
This study aimed to explore the relationship between plasma interleukin 6 (IL-6) levels, adverse cardiovascular events, and the severity of acute coronary syndrome (ACS). A literature review was performed of studies regarding IL-6 and ACS extracted from databases including EMBASE, Cqvip, MEDLINE, Web of Knowledge, PubMed, Cochrane Library, China National Knowledge Infrastructure, and Wanfang data. The Newcastle-Ottawa scale (NOS) was used to evaluate the quality of the literature. The literature was screened, its quality was evaluated, and relevant data were extracted for performing meta-analysis using RevMan software (version 5.3). A total of 524 studies were included in the initial survey. After several rounds of screening and analysis, six studies met the inclusion criteria and underwent meta-analysis using a fixed-effect model. Patients were divided into non-severe and severe groups based on the concentration of high-sensitivity C-reactive protein. Meta-analysis of the relationship between IL-6 and the severity of ACS showed that the plasma IL-6 level of patients in the severe group was significantly higher than that of patients in the non-severe group (p<0.00001). Additionally, patients with experience of major adverse cardiovascular events had significantly higher plasma IL-6 levels than did patients without experience of such events (p<0.00001). In summary, patients with ACS and high IL-6 levels tended to be in a critical condition, with a higher risk of adverse cardiovascular events and worse prognosis. Thus, IL-6 levels could indicate whether patients with ACS may have adverse cardiovascular events and determine the severity of ACS.
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Humanos , Interleucina-6 , Síndrome Coronario Agudo , Pronóstico , Proteína C-Reactiva , ChinaRESUMEN
Abstract To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank: XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous with EPSPS from other plants. qRT-PCR analysis showed that the FvEPSPS was expressed extensively in all tissues including leaves, roots and stems, with higher expression in leaves. Furthermore, transgenic Arabidopsis Thaliana exhibited 10 mM glyphosate to resistance. Therefore, this research offers a new glyphosate-resistant gene for development of transgenic crops.
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Plantas Modificadas Genéticamente , Arabidopsis , Fragaria , Herbicidas/efectos adversosRESUMEN
Sevoflurane (SEVO) is widely applied as an anesthetic, which exerts antitumor capacity in various cancers, including hepatocellular carcinoma (HCC). Previous studies indicated that long non-coding RNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) was upregulated, while microRNA-29a-3p (miR-29a-3p) was downregulated in HCC. Thus, we aimed to explore the roles of KCNQ1OT1 and miR-29a-3p in HCC cells exposed to SEVO. Cell proliferation, apoptosis, migration, and invasion were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, and transwell assays, respectively. The levels of genes were determined by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Furthermore, the interaction between miR-29a-3p and KCNQ1OT1 or chromebox protein homolog 3 (CBX3) was predicted by Starbase or Targetscan, and then confirmed by dual-luciferase reporter assay. We found that the levels of KCNQ1OT1 and CBX3 were decreased, while miR-29a-3p was increased in SEVO-treated HCC cells. KCNQ1OT1 overexpression weakened the inhibitory effects of SEVO on HCC cell proliferation, apoptosis, migration, and invasion. Interestingly, KCNQ1OT1 bound to miR-29a-3p, and miR-29a-3p targeted CBX3. KCNQ1OT1 upregulated CBX3 level by repressing miR-29a-3p expression. Furthermore, KCNQ1OT1 exerted tumor promotion in HCC cells via suppressing miR-29a-3p to regulate CBX3 expression. Collectively, our findings demonstrated that KCNQ1OT1 regulated the antitumor effects of SEVO on HCC cells through modulating the miR-29a-3p/CBX3 axis, providing a theoretical basis for the treatment of HCC.
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Humanos , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/tratamiento farmacológico , Canales de Potasio con Entrada de Voltaje , MicroARNs/genética , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/tratamiento farmacológico , Proteínas Cromosómicas no Histona , ARN Largo no Codificante/genética , Sevoflurano/farmacologíaRESUMEN
BACKGROUND: Obsessive-compulsive disorder (OCD) has a lifetime prevalence of 2-3% and is a leading cause of global disability. Brain circuit abnormalities in individuals with OCD have been identified, but important knowledge gaps remain. The goal of the new global initiative described in this paper is to identify robust and reproducible brain signatures of measurable behaviors and clinical symptoms that are common in individuals with OCD. A global approach was chosen to accelerate discovery, to increase rigor and transparency, and to ensure generalizability of results. METHODS: We will study 250 medication-free adults with OCD, 100 unaffected adult siblings of individuals with OCD, and 250 healthy control subjects at five expert research sites across five countries (Brazil, India, Netherlands, South Africa, and the U.S.). All participants will receive clinical evaluation, neurocognitive assessment, and magnetic resonance imaging (MRI). The imaging will examine multiple brain circuits hypothesized to underlie OCD behaviors, focusing on morphometry (T1-weighted MRI), structural connectivity (Diffusion Tensor Imaging), and functional connectivity (resting-state fMRI). In addition to analyzing each imaging modality separately, we will also use multi-modal fusion with machine learning statistical methods in an attempt to derive imaging signatures that distinguish individuals with OCD from unaffected siblings and healthy controls (Aim #1). Then we will examine how these imaging signatures link to behavioral performance on neurocognitive tasks that probe these same circuits as well as to clinical profiles (Aim #2). Finally, we will explore how specific environmental features (childhood trauma, socioeconomic status, and religiosity) moderate these brain-behavior associations. DISCUSSION: Using harmonized methods for data collection and analysis, we will conduct the largest neurocognitive and multimodal-imaging study in medication-free subjects with OCD to date. By recruiting a large, ethno-culturally diverse sample, we will test whether there are robust biosignatures of core OCD features that transcend countries and cultures. If so, future studies can use these brain signatures to reveal trans-diagnostic disease dimensions, chart when these signatures arise during development, and identify treatments that target these circuit abnormalities directly. The long-term goal of this research is to change not only how we conceptualize OCD but also how we diagnose and treat it.
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Mapeo Encefálico , Encéfalo/diagnóstico por imagen , Imagen de Difusión Tensora , Internacionalidad , Imagen por Resonancia Magnética , Estudios Multicéntricos como Asunto/métodos , Trastorno Obsesivo Compulsivo/diagnóstico por imagen , Adolescente , Adulto , Encéfalo/patología , Encéfalo/fisiopatología , Brasil , Estudios de Casos y Controles , Femenino , Humanos , India , Masculino , Persona de Mediana Edad , Países Bajos , Trastorno Obsesivo Compulsivo/patología , Trastorno Obsesivo Compulsivo/fisiopatología , Proyectos de Investigación , Hermanos/psicología , Sudáfrica , Estados Unidos , Adulto JovenRESUMEN
Not much is known about the roles of long non-coding RNAs (lncRNAs) for chronic kidney disease (CKD). In this study, we included CKD patient cohorts and normal controls as a discovery cohort to identify putative lncRNA biomarkers associated with CKD. We first compared the lncRNA expression profiles of CKD patients with normal controls, and identified differentially expressed lncRNAs and mRNAs. Co-expression network based on the enriched differentially expressed mRNAs and lncRNAs was constructed using WGCNA to identify important modules related to CKD. A lncRNA-miRNA-mRNA pathway network based on the hub lncRNAs and mRNAs, related miRNAs, and overlapping pathways was further constructed to reveal putative biomarkers. A total of 821 significantly differentially expressed mRNAs and lncRNAs were screened between CKD and control samples, which were enriched in nine modules using weighted correlation network analysis (WGCNA), especially brown and yellow modules. Co-expression network based on the enriched differentially expressed mRNAs and lncRNAs in brown and yellow modules uncovered 7 hub lncRNAs and 53 hub mRNAs. A lncRNA-miRNA-mRNA pathway network further revealed that lncRNAs of HCP5 and NOP14-AS1 and genes of CCND2, COL3A1, COL4A1, and RAC2 were significantly correlated with CKD. The lncRNAs of NOP14-AS1 and HCP5 were potential prognostic biomarkers for predicting the risk of CKD.
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Marcadores Genéticos/genética , ARN Largo no Codificante/genética , ARN Mensajero/genética , Insuficiencia Renal Crónica/genética , Estudios de Casos y Controles , Perfilación de la Expresión Génica , Humanos , Tamizaje Masivo , Pronóstico , Insuficiencia Renal Crónica/diagnósticoRESUMEN
Not much is known about the roles of long non-coding RNAs (lncRNAs) for chronic kidney disease (CKD). In this study, we included CKD patient cohorts and normal controls as a discovery cohort to identify putative lncRNA biomarkers associated with CKD. We first compared the lncRNA expression profiles of CKD patients with normal controls, and identified differentially expressed lncRNAs and mRNAs. Co-expression network based on the enriched differentially expressed mRNAs and lncRNAs was constructed using WGCNA to identify important modules related to CKD. A lncRNA-miRNA-mRNA pathway network based on the hub lncRNAs and mRNAs, related miRNAs, and overlapping pathways was further constructed to reveal putative biomarkers. A total of 821 significantly differentially expressed mRNAs and lncRNAs were screened between CKD and control samples, which were enriched in nine modules using weighted correlation network analysis (WGCNA), especially brown and yellow modules. Co-expression network based on the enriched differentially expressed mRNAs and lncRNAs in brown and yellow modules uncovered 7 hub lncRNAs and 53 hub mRNAs. A lncRNA-miRNA-mRNA pathway network further revealed that lncRNAs of HCP5 and NOP14-AS1 and genes of CCND2, COL3A1, COL4A1, and RAC2 were significantly correlated with CKD. The lncRNAs of NOP14-AS1 and HCP5 were potential prognostic biomarkers for predicting the risk of CKD.
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Humanos , ARN Mensajero/genética , Marcadores Genéticos/genética , Insuficiencia Renal Crónica/genética , ARN Largo no Codificante/genética , Pronóstico , Estudios de Casos y Controles , Tamizaje Masivo , Perfilación de la Expresión Génica , Insuficiencia Renal Crónica/diagnósticoRESUMEN
Maize was domesticated from lowland teosinte (Zea mays ssp. parviglumis), but the contribution of highland teosinte (Zea mays ssp. mexicana, hereafter mexicana) to modern maize is not clear. Here, two genomes for Mo17 (a modern maize inbred) and mexicana are assembled using a meta-assembly strategy after sequencing of 10 lines derived from a maize-teosinte cross. Comparative analyses reveal a high level of diversity between Mo17, B73, and mexicana, including three Mb-size structural rearrangements. The maize spontaneous mutation rate is estimated to be 2.17 × 10-8 ~3.87 × 10-8 per site per generation with a nonrandom distribution across the genome. A higher deleterious mutation rate is observed in the pericentromeric regions, and might be caused by differences in recombination frequency. Over 10% of the maize genome shows evidence of introgression from the mexicana genome, suggesting that mexicana contributed to maize adaptation and improvement. Our data offer a rich resource for constructing the pan-genome of Zea mays and genetic improvement of modern maize varieties.
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Evolución Molecular , Genoma de Planta/genética , Zea mays/genética , HaplotiposRESUMEN
ABSTRACTIn this study, ten trace elements in Ziziphora clinopodioidesLam., Lamiaceae, from different regions, periods and parts in Xinjiang were determined by atomic absorption spectrometry following microwave-assisted acid digestion. The decreasing sequence of elements levels was K > Ca > Mg > Fe > Cu > Zn > Na > Mn > Cd > Pb. Chemometric approaches, such as correlation analysis, principal component analysis, and hierarchical cluster analysis were applied to classify Z. clinopodioides according to its elements contents. Principal component analysis revealed 83.51% of the variance with the first four principal component variables. Hierarchical cluster analysis indicated five groups from the eighteen regions, and the result of classification can correspond to the geographical distribution for the most regions. Variation in the elements exhibited a decreasing trend, but of different types in the studied periods. Elemental contents distributed in leaves were higher than those in flowers and stems. Therefore, chemometric approaches could be used to analyze data to accurately classify Z. clinopodioides according to origins. This study provided some elemental information on chemotaxonomy, diversity, changing pattern, distribution, and metabolism of Z. clinopodioides at spatial and temporal levels, and could be used as a reference of planting and quality standards.
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The mitogenic effects of periodic mechanical stress on chondrocytes have been studied extensively but the mechanisms whereby chondrocytes sense and respond to periodic mechanical stress remain a matter of debate. We explored the signal transduction pathways of chondrocyte proliferation and matrix synthesis under periodic mechanical stress. In particular, we sought to identify the role of the MEK1/2-ERK1/2 signaling pathway in chondrocyte proliferation and matrix synthesis following cyclic physiologic mechanical compression. Under periodic mechanical stress, both rat chondrocyte proliferation and matrix synthesis were significantly increased (P < 0.05) and were associated with increases in the phosphorylation of Src, PLCγ1, MEK1/2, and ERK1/2 (P < 0.05). Pretreatment with the MEK1/2-ERK1/2 selective inhibitor, PD98059, and shRNA targeted to ERK1/2 reduced periodic mechanical stress-induced chondrocyte proliferation and matrix synthesis (P < 0.05), while the phosphorylation levels of Src-Tyr418 and PLCγ1-Tyr783 were not inhibited. Proliferation, matrix synthesis and phosphorylation of MEK1/2-Ser217/221 and ERK1/2-Thr202/Tyr204 were inhibited after pretreatment with the PLCγ1 inhibitor U73122 in chondrocytes in response to periodic mechanical stress (P < 0.05), while the phosphorylation site of Src-Tyr418 was not affected. Inhibition of Src activity with PP2 and shRNA targeted to Src abrogated chondrocyte proliferation and matrix synthesis (P < 0.05) and attenuated PLCγ1, MEK1/2 and ERK1/2 activation in chondrocytes subjected to periodic mechanical stress (P < 0.05). These findings suggest that periodic mechanical stress promotes chondrocyte proliferation and matrix synthesis in part through the Src-PLCγ1-MEK1/2-ERK1/2 signaling pathway, which links these three important signaling molecules into a mitogenic cascade.