RESUMEN
PURPOSE: To evaluate the therapeutic efficacy and toxicity of hyperthermic intraperitoneal chemotherapy (HIPEC) plus high-frequency diathermic therapy (HFDT) followed by intravenous chemotherapy vs intravenous chemotherapy alone for adjuvant treatment of postoperative gastrointestinal neoplasms. METHODS: Fifty-two gastrointestinal carcinoma patients who were radically operated were enrolled and divided into the treatment group and the control group. In the treatment group, 25 patients were treated with combination of HIPEC+HFDT and subsequent intravenous chemotherapy, while in the control group 27 patients received intravenous chemotherapy alone. Post-therapeutic complications and adverse reactions, time to progression (TTP) and overall survival (OS) were compared between these two groups. RESULTS: Difference in toxic reactions between the two groups was not statistically significant (p>0.05). Postoperative progression- free survival (PFS) rate at 12 and 40 months after radical surgery was 72.0 and 54.0% respectively in the treatment group, and 65.8 and 11.5% respectively in the control group (p=0.108). TTP was statistically significantly longer in the treatment group than in the control group (median TTP 40.1 vs 18.5 months, p=0.027). Postoperative OS at 12 and 20 months after radical surgery was 88.0 and 78.0% respectively in the treatment group and 92.6 and 72.7% in the control group, without significant difference. CONCLUSION: After radical surgery, combination of HIPEC+HFDT and subsequent intravenous chemotherapy brings about superior PFS compared with intravenous adjuvant chemotherapy alone, while having no more complications and adverse reactions.
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/administración & dosificación , Carcinoma/terapia , Diatermia , Procedimientos Quirúrgicos del Sistema Digestivo , Neoplasias Gastrointestinales/terapia , Adulto , Anciano , Anciano de 80 o más Años , Protocolos de Quimioterapia Combinada Antineoplásica/efectos adversos , Carcinoma/mortalidad , Carcinoma/patología , Quimioterapia Adyuvante , Diatermia/efectos adversos , Diatermia/mortalidad , Procedimientos Quirúrgicos del Sistema Digestivo/efectos adversos , Procedimientos Quirúrgicos del Sistema Digestivo/mortalidad , Progresión de la Enfermedad , Supervivencia sin Enfermedad , Femenino , Neoplasias Gastrointestinales/mortalidad , Neoplasias Gastrointestinales/patología , Humanos , Infusiones Intravenosas , Estimación de Kaplan-Meier , Masculino , Persona de Mediana Edad , Perfusión , Estudios Retrospectivos , Factores de Tiempo , Resultado del TratamientoRESUMEN
Diabetic nephropathy (DN) is the major life-threatening complication of diabetes. Abnormal permeability of glomerular basement membrane plays an important role in DN pathogenesis. This study was performed to assess the effect of arctiin, the lignan constituent from Arctium lappa L., on metabolic profile and aggravation of renal lesions in a rat model of streptozotocin (STZ)-induced DN. STZ-induced diabetic rats were treated with arctiin at the dosage of 60 or 40 mg/kg/day via intraperitoneal injection for 8 weeks. Blood glucose and 24-h urinary albumin content were measured, and kidney histopathological changes were monitored. RT-PCR and immunohistochemistry were used to detect the mRNA and protein levels of nephrin, podocin and heparanase (HPSE) in the kidney cortex of rats, respectively. Treatment with arctiin significantly decreased the levels of 24-h urinary albumin, prevented the sclerosis of glomeruli and effectively restored the glomerular filtration barrier damage by up-regulating the expression of nephrin and podocin and down-regulating HPSE level. Our studies suggest that arctiin might be beneficial for DN. The effects of arctiin on attenuating albuminuria and glomerulosclerosis are possibly mediated by regulating the expression of nephrin and podocin and HPSE in STZ-induced diabetic rats.
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Diabetes Mellitus Experimental/tratamiento farmacológico , Nefropatías Diabéticas/tratamiento farmacológico , Furanos/farmacología , Barrera de Filtración Glomerular/efectos de los fármacos , Glucósidos/farmacología , Albuminuria/metabolismo , Animales , Glucemia/metabolismo , Diabetes Mellitus Experimental/fisiopatología , Nefropatías Diabéticas/fisiopatología , Regulación de la Expresión Génica/efectos de los fármacos , Glucuronidasa/metabolismo , Péptidos y Proteínas de Señalización Intracelular/metabolismo , Glomérulos Renales/efectos de los fármacos , Glomérulos Renales/patología , Masculino , Proteínas de la Membrana/metabolismo , Metaboloma , ARN Mensajero/metabolismo , Ratas , Ratas Sprague-Dawley , EstreptozocinaRESUMEN
The optimal conditions of total flavonoids extraction from Mentha spicata were identified by orthogonal test. The results showed that the ethanol concentration and the ultrasonic time had significant effect on the extraction of total flavonoids and the optimum conditions of extraction were A3B3C1D1, which were as follows: the ethanol concentration was 50%, the ultrasonic time was 40 min, the solid-liquid ratio was 1 g: 50 mL and the temperature of extraction was 60 degrees C, On the basis of the extracting method above, the content of total flavonoids in Mentha spicata was 0.0951 mg/mg. The linear equation was A = 11.51143C - 0.00029, r = 0.9999. This method is simple, quick and accurate with good prosperity.
Asunto(s)
Flavonoides/aislamiento & purificación , Mentha/química , Plantas Medicinales/química , Tecnología Farmacéutica/métodos , Ultrasonido , Etanol/química , Tecnología Farmacéutica/instrumentación , Temperatura , Factores de TiempoRESUMEN
A new method using microwave digestion technique was developed for the determination of lead in Astragalus by CTAB enhancing-continual flow hydride generation-inductively coupled plasma atomic emission spectrometry (HG-ICP-AES). The experimental conditions of microwave digestion and hydride generation were optimized. This method shows a linear range of 0.23-800 microg x L(-1) and the correlation coefficient is 0.999 9. It is satisfactory to apply the microwave digestion procedure to the determination of Pb under the optimized conditions. The detection limit of the method is 0.23 microg x L(-1) and the RSD is 1.02%. The recovery obtained is 98.8%-100.1%. The results show that this method is rapid and simple with low environmental contamination and complete digestion of samples.