RESUMEN

The immune system employs soluble effectors to shape luminal spaces. Antibodies are soluble molecules that effect immunological responses, including neutralization, opsonization, antibody-dependent cytotoxicity and complement activation. These molecules are comprised of immunoglobulin (Ig) domains. The N-terminal Ig domains recognize antigen, and the C-terminal domains facilitate their elimination through phagocytosis (opsonization). A less-recognized function mediated by the C-terminal Ig domains of the IgG class of antibodies (Fc region) involves the formation of multiple low-affinity bonds with the mucus matrix. This association anchors the antibody molecule to the matrix to entrap potential pathogens. Even though invertebrates are not known to have antibodies, protochordates have a class of secreted molecules containing Ig domains that can bind bacteria and potentially serve a similar purpose. The VCBPs (V region-containing chitin-binding proteins) possess a C-terminal chitin-binding domain that helps tether them to chitin-rich mucus gels, mimicking the IgG-mediated Fc trapping of microbes in mucus. The broad functional similarity of these structurally divergent, Ig-containing, secreted effectors makes a case for a unique form of convergent evolution within chordates. This opinion essay highlights emerging evidence that divergent secreted immune effectors with Ig-like domains evolved to manage immune recognition at mucosal surfaces in strikingly similar ways. This article is part of the theme issue 'Sculpting the microbiome: how host factors determine and respond to microbial colonization'.

Asunto(s)

Quitina , Mucinas , Transporte Biológico , Inmunoglobulina G

RESUMEN

Immune inhibitory receptor genes that encode a variable (V) region, a unique V-like C2 (V/C2) domain, a transmembrane region, and a cytoplasmic tail containing immunoreceptor tyrosine-based inhibition motifs (ITIMs) have been described previously in two lineages of bony fish. In the present study, eleven related genes encoding distinct structural forms have been identified in Ictalurus punctatus (channel catfish), a well characterized immunological model system that represents a third independent bony fish lineage. Each of the different genes encodes an N-terminal V region but differs in the number of extracellular Ig domains, number and location of joining (J) region-like motifs, presence of transmembrane regions, presence of charged residues in transmembrane regions, presence of cytoplasmic tails, and/or distribution of ITIM(s) within the cytoplasmic tails. Variation in the numbers of genomic copies of the different gene types, their patterns of expression, and relative levels of expression in mixed leukocyte cultures (MLC) is reported. V region-containing immune-type genes constitute a far more complex family than recognized originally and include individual members that might function in inhibitory or, potentially activatory manners.

Asunto(s)

Variación Genética , Región Variable de Inmunoglobulina/genética , Receptores Inmunológicos/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , ADN Complementario , Evolución Molecular , Expresión Génica , Ictaluridae , Región Variable de Inmunoglobulina/clasificación , Prueba de Cultivo Mixto de Linfocitos , Datos de Secuencia Molecular , Receptores Inmunológicos/clasificación

RESUMEN

Novel immune-type receptor (NITR) genes, which initially were identified in the Southern pufferfish (Spheroides nephelus), encode products which consist of an extracellular variable (V) and V-like C2 (V/C2) domain, a transmembrane region, and a cytoplasmic tail, which typically possesses an immunoreceptor tyrosine-based inhibition motif (ITIM). Multiple NITR genes have been identified in close, contiguous chromosomal linkage. The V regions of NITRs resemble prototypic forms defined for immunoglobulin (Ig) and T-cell antigen receptor (TCR), are present in multiple families and exhibit regionalized variation in sequence, which also occurs in Ig and TCR. Comparisons of exons encoding transmembrane and cytoplasmic regions of multiple NITRs suggest that exon shuffling has factored in the diversification of the NITR gene complex. Zebrafish (Danio rerio) NITRs exhibit many of these characteristics. NITRs that have been identified in additional species of bony fish demonstrate additional variation in the number of extracellular domains as well as in the presence of intramembranous charged residues, cytoplasmic tails and ITIMs. The presence in NITRs of V regions that are related closely to those found in Ig and TCR, as well as regulatory motifs and other structural features that are characteristic of immune inhibitory receptors encoded at the leukocyte receptor cluster, suggests that the NITRs are representative of an integral stage in the evolution of innate and adaptive immune function.

Asunto(s)

Peces/genética , Peces/inmunología , Receptores Inmunológicos/genética , Animales , Evolución Biológica , Variación Genética , Genoma , Modelos Biológicos , Estructura Molecular , Reacción en Cadena de la Polimerasa , Receptores Inmunológicos/química

RESUMEN

We describe the generation of a P1 artificial chromosome genomic library from the Southern pufferfish, Spheroides nephelus. The arrayed library consists of approximately 30,000 clones and has an average insert size of 125-150 kb. The coverage is estimated to encompass seven to eight genome equivalents. The library has been used for isolating numerous genomic clones and for establishing contigs of several multigene families. Analysis of several of the clones from this library suggests a preponderance of CA repeat tracts relative to their abundance in humans. The library and high-density filters have been made available to the scientific public through genomics distribution companies.

Asunto(s)

Bacteriófago P1/genética , Peces/genética , Biblioteca Genómica , Animales , Cromosomas Artificiales/genética , Clonación Molecular , ADN/genética , Dermatoglifia del ADN , Repeticiones de Dinucleótido/genética , Genoma

RESUMEN

An extensive, highly diversified multigene family of novel immune-type receptor (nitr) genes has been defined in Danio rerio (zebrafish). The genes are predicted to encode type I transmembrane glycoproteins consisting of extracellular variable (V) and V-like C2 (V/C2) domains, a transmembrane region and a cytoplasmic tail. All of the genes examined encode immunoreceptor tyrosine-based inhibition motifs in the cytoplasmic tail. Radiation hybrid panel mapping and analysis of a deletion mutant line (b240) indicate that a minimum of approximately 40 nitr genes are contiguous in the genome and span approximately 0.6 Mb near the top of zebrafish linkage group 7. One flanking region of the nitr gene complex shares conserved synteny with a region of mouse chromosome 7, which shares conserved synteny with human 19q13.3-q13.4 that encodes the leukocyte receptor cluster. Antibody-induced crosslinking of Nitrs that have been introduced into a human natural killer cell line inhibits the phosphorylation of mitogen-activated protein kinase that is triggered by natural killer-sensitive tumor target cells. Nitrs likely represent intermediates in the evolution of the leukocyte receptor cluster.

Asunto(s)

Familia de Multigenes , Receptores Inmunológicos/genética , Pez Cebra/genética , Secuencia de Aminoácidos , Animales , Evolución Biológica , Secuencia Conservada , Genes de Inmunoglobulinas , Ligamiento Genético , Células Asesinas Naturales/inmunología , Datos de Secuencia Molecular , Pez Cebra/inmunología

RESUMEN

Cartilaginous fish express canonical B and T cell recognition genes, but their lymphoid organs and lymphocyte development have been poorly defined. Here, the expression of Ig, TCR, recombination-activating gene (Rag)-1 and terminal deoxynucleosidase (TdT) genes has been used to identify roles of various lymphoid tissues throughout development in the cartilaginous fish, Raja eglanteria (clearnose skate). In embryogenesis, Ig and TCR genes are sharply up-regulated at 8 weeks of development. At this stage TCR and TdT expression is limited to the thymus; later, TCR gene expression appears in peripheral sites in hatchlings and adults, suggesting that the thymus is a source of T cells as in mammals. B cell gene expression indicates more complex roles for the spleen and two special organs of cartilaginous fish-the Leydig and epigonal (gonad-associated) organs. In the adult, the Leydig organ is the site of the highest IgM and IgX expression. However, the spleen is the first site of IgM expression, while IgX is expressed first in gonad, liver, Leydig and even thymus. Distinctive spatiotemporal patterns of Ig light chain gene expression also are seen. A subset of Ig genes is pre-rearranged in the germline of the cartilaginous fish, making expression possible without rearrangement. To assess whether this allows differential developmental regulation, IgM and IgX heavy chain cDNA sequences from specific tissues and developmental stages have been compared with known germline-joined genomic sequences. Both non-productively rearranged genes and germline-joined genes are transcribed in the embryo and hatchling, but not in the adult.

Asunto(s)

Rajidae/genética , Animales , Linfocitos B , ADN Nucleotidilexotransferasa/genética , Expresión Génica , Gónadas/inmunología , Proteínas de Homeodominio/genética , Cadenas Ligeras de Inmunoglobulina/genética , Inmunoglobulina M/genética , Inmunoglobulinas/genética , Reacción en Cadena de la Polimerasa , Receptores de Antígenos de Linfocitos T alfa-beta/genética , Receptores de Antígenos de Linfocitos T gamma-delta/genética , Rajidae/crecimiento & desarrollo , Rajidae/inmunología , Bazo/inmunología , Timo/inmunología , Transposasas/genética

RESUMEN

T lymphocytes and B lymphocytes are present in jawed vertebrates, including cartilaginous fishes, but not in jawless vertebrates or invertebrates. The origins of these lineages may be understood in terms of evolutionary changes in the structure and regulation of transcription factors that control lymphocyte development, such as PU.1. The identification and characterization of three members of the PU.1 family of transcription factors in a cartilaginous fish, Raja eglanteria, are described here. Two of these genes are orthologs of mammalian PU.1 and Spi-C, respectively, whereas the third gene, Spi-D, is a different family member. In addition, a PU.1-like gene has been identified in a jawless vertebrate, Petromyzon marinus (sea lamprey). Both DNA-binding and transactivation domains are highly conserved between mammalian and skate PU.1, in marked contrast to lamprey Spi, in which similarity is evident only in the DNA-binding domain. Phylogenetic analysis of sequence data suggests that the appearance of Spi-C may predate the divergence of the jawed and jawless vertebrates and that Spi-D arose before the divergence of the cartilaginous fish from the lineage leading to the mammals. The tissue-specific expression patterns of skate PU.1 and Spi-C suggest that these genes share regulatory as well as structural properties with their mammalian orthologs.

Asunto(s)

Evolución Biológica , Proteínas de Unión al ADN/genética , Hematopoyesis , Familia de Multigenes , Isoformas de Proteínas/genética , Proteínas Proto-Oncogénicas/genética , Rajidae/genética , Transactivadores/genética , Factores de Transcripción/genética , Proteínas de Xenopus , Secuencia de Aminoácidos , Animales , Sitios de Unión , Pollos/genética , ADN/metabolismo , ADN Complementario/genética , Evolución Molecular , Peces/clasificación , Peces/genética , Peces/inmunología , Genes , Hematopoyesis/genética , Humanos , Invertebrados/genética , Invertebrados/inmunología , Lampreas/genética , Lampreas/inmunología , Subgrupos Linfocitarios/inmunología , Ratones , Datos de Secuencia Molecular , Especificidad de Órganos , Filogenia , Isoformas de Proteínas/metabolismo , Proteínas Proto-Oncogénicas/clasificación , Proteínas Proto-Oncogénicas/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Rajidae/inmunología , Especificidad de la Especie , Bazo/química , Transactivadores/clasificación , Transactivadores/metabolismo , Vertebrados/clasificación , Vertebrados/genética , Vertebrados/inmunología

RESUMEN

The zebrafish (Danio rerio) has become a significant model for understanding the developmental regulation of gene expression and holds considerable potential for characterizing the development of the immune system. Using a number of different approaches, including heterologous hybridization and short-primer PCR, cDNAs for three different classes of light-chain genes were identified and characterized. The zebrafish light chains are similar to trout type 1, trout type 2, and catfish type F, respectively. T-cell antigen receptor alpha (TCRalpha) was also identified and characterized. A high proportion of unusual transcripts including sterile transcripts, germline VJC transcripts, aberrant splice forms, and V-V transcripts were encountered in the immunoglobulin and TCR cDNAs examined. The light-chain and TCRalpha loci each consist of multiple families of V gene segments, apparent even from the small numbers of cDNAs of each isotype sequenced. The gene sequences reported provide an essential set of markers of both B- and T-cell lineages that will facilitate investigations of immune system development.

Asunto(s)

Genes Codificadores de la Cadena alfa de los Receptores de Linfocito T , Regiones Constantes de Inmunoglobulina/genética , Región de Unión de la Inmunoglobulina/genética , Cadenas Ligeras de Inmunoglobulina/genética , Región Variable de Inmunoglobulina/genética , Pez Cebra/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , ADN Complementario , Regiones Constantes de Inmunoglobulina/clasificación , Isotipos de Inmunoglobulinas/clasificación , Isotipos de Inmunoglobulinas/genética , Región de Unión de la Inmunoglobulina/clasificación , Cadenas Ligeras de Inmunoglobulina/clasificación , Región Variable de Inmunoglobulina/clasificación , Datos de Secuencia Molecular , Filogenia , Homología de Secuencia de Aminoácido , Pez Cebra/inmunología

RESUMEN

The extraembryonic ectoderm development (exed) mutant phenotype was described in mice homozygous for the c(6H) deletion, a radiation-induced deletion in the tyrosinase region of mouse Chromosome 7. These mutants fail to gastrulate and die around embryonic day 8.0. Several genes including, for example, embryonic ectoderm development (eed), are deleted in the c(6H) mutants; however, the portion of the chromosome responsible for the more severe exed phenotype is localized to a 20-kb region called the "exed-critical region." To understand the genetics behind the exed phenotype, we analyzed this region in two ways. First, to determine whether the 20-kb exed-critical region alone causes the mutant phenotype, we removed it from a wild-type chromosome. The resulting mice homozygous for this deletion were viable and fertile, indicating that the 20-kb exed-critical region by itself is not sufficient to cause the phenotype when deleted. We then sequenced the 20-kb exed-critical region and no expressed exons were found. Several short matches to GenBank Expressed Sequence Tag (EST) databases were identified; however, none of these ESTs mapped to the region. Taken together, these results indicate that the exed phenotype may either be a position effect on a distal gene caused by the c(6H) breakpoint or the result of composite effects of nullizygosity of multiple genes in the deletion homozygotes.

Asunto(s)

Ectodermo/fisiología , Ratones Mutantes/embriología , Ratones Mutantes/genética , Animales , Línea Celular , Embrión de Mamíferos/citología , Femenino , Regulación del Desarrollo de la Expresión Génica/genética , Regulación del Desarrollo de la Expresión Génica/fisiología , Masculino , Ratones , Ratones Endogámicos C57BL , Fenotipo , Células Madre/fisiología

RESUMEN

Members of the Ikaros multigene family of zinc finger proteins are expressed in a tissue-specific manner and most are critical determinants in the development of both the B and T lymphocytes as well as NK and dendritic APC lineages. A PCR amplification strategy that is based on regions of shared sequence identity in Ikaros multigene family members found in mammals and several other vertebrates has led to the recovery of cDNAs that represent the orthologues of Ikaros, Aiolos, Helios, and Eos in Raja eglanteria (clearnose skate), a cartilaginous fish that is representative of an early divergence event in the phylogenetic diversification of the vertebrates. The tissue-specific patterns of expression for at least two of the four Ikaros family members in skate resemble the patterns observed in mammals, i.e., in hematopoietic tissues. Prominent expression of Ikaros in skate also is found in the lymphoid Leydig organ and epigonal tissues, which are unique to cartilaginous fish. An Ikaros-related gene has been identified in Petromyzon marinus (sea lamprey), a jawless vertebrate species, in which neither Ig nor TCRs have been identified. In addition to establishing a high degree of evolutionary conservation of the Ikaros multigene family from cartilaginous fish through mammals, these studies define a possible link between factors that regulate the differentiation of immune-type cells in the jawed vertebrates and related factors of unknown function in jawless vertebrates.

Asunto(s)

Proteínas de Unión al ADN , Lampreas/genética , Familia de Multigenes/inmunología , Rajidae/genética , Factores de Transcripción/genética , Secuencia de Aminoácidos , Animales , Pollos , Amplificación de Genes , Expresión Génica/inmunología , Humanos , Factor de Transcripción Ikaros , Lampreas/inmunología , Ratones , Datos de Secuencia Molecular , Especificidad de Órganos/genética , Especificidad de Órganos/inmunología , Filogenia , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Rajidae/inmunología , Trucha , Pez Cebra , Proteínas de Pez Cebra , Dedos de Zinc/genética , Dedos de Zinc/inmunología

RESUMEN

Immunoglobulin gene diversity has been characterized to varying degrees in modern representatives of all of the major radiations of cartilaginous fish. A pattern of overall chromosomal relationships of the various types of joined and unjoined Ig gene clusters is suggested in which the essential features are: (a) both Ig heavy and light-chain gene clusters occur on multiple chromosomes, (b) various classes of Ig are interspersed, (c) not all individual gene loci appear to be closely linked (Fig. 2). The cluster-type Ig gene system appears to be a series of (potentially) individually regulated loci analogous in part to the olfactory receptor gene system (BUCK and AXEL 1991) and markedly distinct from Ig loci in other vertebrate groups and TCR genes. Such a system would be ideal for the creation of variation in both form and function in a large number of clusters while preserving or partially preserving specificity in a number of other gene clusters. The full range of joined genes and the relative number of joined genes (as relates to unjoined genes), have yet to be determined. Nevertheless, a number of conclusions can be drawn: (a) four distinct forms of heavy-chain joining have been identified (VDD-J, VD-DJ, V-D-DJ, and VDJ; Fig. 1); (b) light-chain genes, which possess only two recombining elements, can be found in either unjoined (V-J) or joined (VJ) forms (Fig. 1); (c) physical linkage between individual joined and unjoined genes has not been established, although such investigations have not been pursued in a significantly rigorous manner as to rule out this possibility; (d) joined light-chain genes are expressed and can be somatically mutated. Can germline joining be viewed as an ancestral character? The answer to this needs to be considered in the context of an overall system in which the level of structural and functional redundancy is extremely high. Joining is an adaptation that is unique to multicluster gene families. The phenomenon overcomes the possibility of not generating a specific form of a receptor, a major shortcoming of conventional rearranging Ig and TCR gene systems. The limitation of encoding specific receptors is compensated through large numbers of additional gene clusters that retain the capacity to rearrange and generate new specificities. Commitment of a V region to diverse, fixed specificity also is a property of the NITR genes, which although not related closely to Ig in a structural sense, may reflect an analogous phenomena. The possibility that immune-type diversity is achieved in the absence of somatic rearrangement and that remnants of such systems could be operative in immune recognition in contemporary vertebrates is of extraordinary significance in terms of our overall understanding of the relationships between adaptive and innate immune recognition.

Asunto(s)

Diversidad de Anticuerpos , Animales , Evolución Biológica , Peces/genética , Peces/inmunología , Reordenamiento Génico , Genes de Inmunoglobulinas

RESUMEN

In addition to being an excellent model system for studying vertebrate development, the zebrafish has become a great tool for gene discovery by mutational analysis. The recent availability of the zebrafish EST database and radiation hybrid mapping panels has dramatically expanded the framework for genomic research in this species. Developing comparative maps of the zebrafish and human genomes is of particular importance for zebrafish mutagenesis studies in which human orthologs are sought for zebrafish genes. However, only partial cDNA sequences are determined routinely for mapped ESTs, leaving the identity of the EST in question. It previously had been reported that zebrafish linkage group 7 shares conserved synteny with human chromosome 11q13. In an effort to further define this relationship, five full-length zebrafish cDNAs, fth1, slc3a2, prkri, cd81, and pc, as well as one putative human gene, DBX were identified and their map positions ascertained. These six genes, along with men1, fgf3 and cycd1 define two regions of conserved synteny between linkage group 7 and 11q13.

Asunto(s)

Cromosomas Humanos Par 11/genética , Genes/genética , Proteínas de la Membrana , Pez Cebra/genética , Secuencia de Aminoácidos , Animales , Antígenos CD/genética , Proteínas Portadoras/genética , Mapeo Cromosómico , Secuencia Conservada , Etiquetas de Secuencia Expresada , Ferritinas/genética , Proteína-1 Reguladora de Fusión , Proteínas del Choque Térmico HSP40 , Proteínas de Homeodominio/genética , Humanos , Datos de Secuencia Molecular , Proteínas Serina-Treonina Quinasas/antagonistas & inhibidores , Piruvato Carboxilasa/genética , Mapeo de Híbrido por Radiación , Proteínas Represoras/genética , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Tetraspanina 28

RESUMEN

The immunoglobulin superfamily (IgSF) is an extensively diversified multigene family whose members share a common structural feature, the Ig fold. Members of the Ig/T-cell antigen receptor (TCR) subset of the IgSF mediate antigen-specific recognition in adaptive immune responses. Antigen-binding receptors belonging to this subset are present in all species of jawed vertebrates. To explore whether there are additional structurally related but otherwise distinct members of this subset, we have developed a technique termed the short-primer polymerase chain reaction (PCR) that targets structurally conserved short motifs in the Ig fold. Large-scale sequencing efforts and recent advances in information biotechnology, including "electronic PCR," provide additional computational means to implement similarly directed searches within databases. The use of these approaches has led to the discoveries of Ig/TCR homologues in a variety of phylogenetically diverse organisms, a diversified family of novel immune-type receptor genes, as well as a novel human IgSF member. The potential of random sequencing efforts and virtual screening of databases is described in the context of two novel genes in bony fish. The various methodologies that are discussed and the examples shown provide means for further investigating, and/or elucidating novel, IgSF receptors as well as components of pathways that are involved in immune responses in both traditional and nontraditional model systems.

Asunto(s)

Inmunoglobulinas/genética , Receptores de Antígenos de Linfocitos T/genética , Receptores Inmunológicos/genética , Secuencia de Aminoácidos , Animales , Humanos , Datos de Secuencia Molecular , Familia de Multigenes/genética , Alineación de Secuencia , Homología de Secuencia de Aminoácido

RESUMEN

Antigen recognition in the adaptive immune response by Ig and T-cell antigen receptors (TCRs) is effected through patterned differences in the peptide sequence in the V regions. V-region specificity forms through genetically programmed rearrangement of individual, diversified segmental elements in single somatic cells. Other Ig superfamily members, including natural killer receptors that mediate cell-surface recognition, do not undergo segmental reorganization, and contain type-2 C (C2) domains, which are structurally distinct from the C1 domains found in Ig and TCR. Immunoreceptor tyrosine-based inhibitory motifs that transduce negative regulatory signals through the cell membrane are found in certain natural killer and other cell surface inhibitory receptors, but not in Ig and TCR. In this study, we employ a genomic approach by using the pufferfish (Spheroides nephelus) to characterize a nonrearranging novel immune-type receptor gene family. Twenty-six different nonrearranging genes, which each encode highly diversified V as well as a V-like C2 extracellular domain, a transmembrane region, and in most instances, an immunoreceptor tyrosine-based inhibitory motif-containing cytoplasmic tail, are identified in an approximately 113 kb P1 artificial chromosome insert. The presence in novel immune-type receptor genes of V regions that are related closely to those found in Ig and TCR as well as regulatory motifs that are characteristic of inhibitory receptors implies a heretofore unrecognized link between known receptors that mediate adaptive and innate immune functions.

Asunto(s)

Peces/genética , Genes de Inmunoglobulinas , Genes Codificadores de los Receptores de Linfocitos T , Región Variable de Inmunoglobulina/genética , Familia de Multigenes , Secuencia de Aminoácidos , Animales , ADN Complementario/genética , Evolución Molecular , Peces/inmunología , Tejido Linfoide/citología , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa , Receptores Inmunológicos/genética , Homología de Secuencia de Aminoácido , Células Madre

Asunto(s)

Antígenos de Diferenciación de Linfocitos B/genética , Antígenos de Histocompatibilidad Clase II/genética , Pez Cebra/genética , Secuencia de Aminoácidos , Animales , Antígenos de Diferenciación de Linfocitos B/clasificación , Secuencia de Bases , ADN Complementario/genética , Evolución Molecular , Antígenos de Histocompatibilidad Clase II/clasificación , Datos de Secuencia Molecular , Homología de Secuencia de Aminoácido , Pez Cebra/inmunología

RESUMEN

This review addresses issues related to the evolution of the complex multigene families of antigen binding receptors that function in adaptive immunity. Advances in molecular genetic technology now permit the study of immunoglobulin (Ig) and T cell receptor (TCR) genes in many species that are not commonly studied yet represent critical branch points in vertebrate phylogeny. Both Ig and TCR genes have been defined in most of the major lineages of jawed vertebrates, including the cartilaginous fishes, which represent the most phylogenetically divergent jawed vertebrate group relative to the mammals. Ig genes in cartilaginous fish are encoded by multiple individual loci that each contain rearranging segmental elements and constant regions. In some loci, segmental elements are joined in the germline, i.e. they do not undergo genetic rearrangement. Other major differences in Ig gene organization and the mechanisms of somatic diversification have occurred throughout vertebrate evolution. However, relating these changes to adaptive immune function in lower vertebrates is challenging. TCR genes exhibit greater sequence diversity in individual segmental elements than is found in Ig genes but have undergone fewer changes in gene organization, isotype diversity, and mechanisms of diversification. As of yet, homologous forms of antigen binding receptors have not been identified in jawless vertebrates; however, acquisition of large amounts of structural data for the antigen binding receptors that are found in a variety of jawed vertebrates has defined shared characteristics that provide unique insight into the distant origins of the rearranging gene systems and their relationships to both adaptive and innate recognition processes.

Asunto(s)

Evolución Biológica , Genes de Inmunoglobulinas , Receptores de Antígenos de Linfocitos T/genética , Anfibios/genética , Anfibios/inmunología , Animales , Aves/genética , Aves/inmunología , Peces/genética , Peces/inmunología , Reordenamiento Génico de Linfocito B , Reordenamiento Génico de Linfocito T , Humanos , Memoria Inmunológica , Familia de Multigenes , Mutación , Filogenia , Reptiles/genética , Reptiles/inmunología

Asunto(s)

Proteínas Serina-Treonina Quinasas/genética , Proteínas Proto-Oncogénicas/genética , Pez Cebra/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , ADN Complementario , Humanos , Datos de Secuencia Molecular , Proteínas Proto-Oncogénicas c-pim-1 , Homología de Secuencia de Aminoácido

RESUMEN

Differential screening has been used to identify cDNAs encoding a long form of IgX in Raja eglanteria (clearnose skate). Comparisons of the IgX long form with the previously described short-form IgX cDNAs and the genomic IgX locus indicate that the V and two 5' C regions of the short and long forms of IgX are >90% identical at the nucleotide level. Differences between the V sequences of the long- and short-form IgX genes are concentrated in complementarity determining regions, suggesting that these forms are derived through alternative splicing of the same genomic loci or transcription of highly related loci. The extreme conservation of nucleotide sequence, including third position codons, among different cDNAs as well as the near identity of nucleotide sequence in the intervening sequences of germline IgX, IgX short-form sterile transcripts and IgX long-form sterile transcripts indicate that the multiple IgX loci are recently diverged from one another and/or are under intense gene correction. Phylogenetic analyses of the known cartilaginous fish immunoglobulin loci demonstrate that the long form of IgX is orthologous to IgW/IgNARC (NARC) and is most consistent with: 1) the divergence of the IgX/IgW/NARC and IgM-like loci from a common ancestral locus prior to the divergence of the cartilaginous/bony fish lineages and 2) the divergence of the NAR locus from the IgX/IgW/NARC gene(s) after the cartilaginous/bony fish split but prior to the shark/skate split, approximately 220 million years ago.

Asunto(s)

Genes de Inmunoglobulinas , Cadenas Pesadas de Inmunoglobulina/genética , Tiburones/genética , Rajidae/genética , Secuencia de Aminoácidos , Animales , Diversidad de Anticuerpos , Secuencia de Bases , ADN Complementario/genética , Evolución Molecular , Peces/clasificación , Peces/genética , Isotipos de Inmunoglobulinas , Mamíferos/genética , Datos de Secuencia Molecular , Filogenia , Alineación de Secuencia , Homología de Secuencia de Ácido Nucleico , Rajidae/inmunología , Especificidad de la Especie , Transcripción Genética

Asunto(s)

Riñón/enzimología , Proteínas Tirosina Quinasas/genética , Pez Cebra/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , ADN Complementario/aislamiento & purificación , Expresión Génica , Humanos , Ratones , Datos de Secuencia Molecular , Proteínas Tirosina Quinasas/biosíntesis , Proteínas Tirosina Quinasas/aislamiento & purificación , Alineación de Secuencia , Homología de Secuencia de Aminoácido

RESUMEN

Immunoglobulin heavy chain (IgH) genes in representative chondrichthyan fishes (sharks and skates) consist of independently functioning clusters, containing separate variable (VH), diversity (DH), and joining (JH) region elements and constant (CH) region exons. IgH loci have been characterized in Hydrolagus colliei (spotted ratfish), a modern representative of a major independent chondrichthyan lineage. Three distinct families of IgH gene clusters were identified. The most numerous genes consist of unjoined VH-D1-D2-JH segments that correspond to the most abundant Hydrolagus spleen (cDNA) transcripts which apparently arise from a diversified gene family. In the second cluster type, VH, DH, and JH segments are germline-joined, whereas the CH exons exhibit typical organization. This gene type is found in only a few copies per haploid genome and both transmembrane and secretory transcripts have been identified. A third cluster type has been identified that consists of unjoined VH elements but lacks a typical CH1 exon, which is substituted with a second CH2-like exon. Transcripts from this third cluster type also appear to derive from a diversified gene family. Genomic D regions of the two unjoined clone types exhibit structural differences that are consistent with incorporation of recombination machinery-mediated events. Genomic library screening indicates that 90% of VH+ clones are truncated, nearly identical pseudogenes (lacking JH and CH). These studies demonstrate an early phylogenetic origin for the cluster type of gene organization and document extensive organizational diversification within an apparent single class of IgH genes.

Asunto(s)

Cazón/genética , Cadenas Pesadas de Inmunoglobulina/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Southern Blotting , ADN Complementario , Cazón/inmunología , Electroforesis en Gel de Campo Pulsado , Biblioteca de Genes , Ligamiento Genético , Variación Genética , Fragmentos de Inmunoglobulinas/genética , Región de Unión de la Inmunoglobulina/genética , Región Variable de Inmunoglobulina/genética , Datos de Secuencia Molecular , Familia de Multigenes , Seudogenes , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Transcripción Genética