RESUMEN
OBJECTIVE: To study the differential diagnostic value of histological and immunohistochemical signs in skin biopsy specimens in graft-versus-host disease (GVHD). MATERIAL AND METHODS: Hematoxylin- and eosin-stained skin biopsy specimens from 50 patients with GVHD and 10 patients with toxicoderma were examined; immunohistochemical reactions using antibodies against CD3, CD20, CD4, CD8, FOXP3, CD56, CD1a, and CD68 were performed. The significance of differences between the groups was estimated using the chi2 and Mann-Whitney tests. RESULTS: In GVHD, there was a significantly high count of necrotic epidermocytes with satellite cells in the epidermis, more commonly apoptosis of epitheliocytes in the skin appendages and vacuolar dystrophy of epidermocytes. On the contrary, the count of eosinophilic granulocytes, CD1a+, CD3+, FOXP3+, and CD8+ cells and the proportion of FOXP3+ cells proved to be significantly higher in the toxicoderma group. CONCLUSION: The results of the investigation were used to form groups of signs that were important in the differential diagnosis of cutaneous GVHD and toxic and allergic reactions.
Asunto(s)
Enfermedad Injerto contra Huésped/diagnóstico , Enfermedad Injerto contra Huésped/genética , Enfermedades de la Piel/diagnóstico , Piel/patología , Adulto , Anciano , Antígenos CD/biosíntesis , Biopsia , Femenino , Enfermedad Injerto contra Huésped/patología , Humanos , Masculino , Persona de Mediana Edad , Enfermedades de la Piel/genética , Enfermedades de la Piel/patologíaRESUMEN
We developed a new approach to evaluation of the intensity of osteogenic differentiation of mesenchymal stromal stem cells based on measurement of optical density of mesenchymal stromal cell cultures in a concentration range 625-10,000 cells per well after their culturing in osteogenic induction medium and staining of calcium deposits by the method of von Kossa. The proposed method allows comparative semiquantitative evaluation of osteogenic properties of mesenchymal stem cells depending on tissue sources (bone marrow, adipose tissue, placenta), in vitro cell density, number of passages, duration of culturing, and concentration of serum growth factors in the microenvironment. The developed approach makes it possible to compare functional activity of mesenchymal stromal cells in various pathologies. The proposed method can be used in traumatology and orthopedics for improving the efficiency of transplantation of mesenchymal stromal cells for stimulation of reparative osteogenesis.