RESUMEN
We investigated the genes kpsMTII, iucD, sfaDE, afaBC, papA and papC, (proposed to be involved in extra-intestinal pathogenic Escherichia coli-ExPEC), phylogroup classification and the in vitro swimming and swarming motility in 50 E. coli isolated from bovine mastitis with different clinical severity scores (mild, moderate and severe). The aforementioned genes were detected in 12 (n = 12/50; 24·0%) isolates. kpsMTII and iucD were the most frequent genes identified in six (n = 6/50; 12·0%) and four (n = 4/50; 8·0%) of the isolates, respectively. In only one (n = 1/50; 2·0%) isolate, more than one gene was simultaneously identified: iucD and kpsMTll were detected whereas sfaDE and afaBC were not detected. Mild, moderate and severe clinical signs were observed in 40·0% (n = 20/50), 28·0% (n = 14/50) and 32·0% (n = 16/50) of the cases. Commensal phylogroups B1 (n = 19/50; 38·0%) and A (n = 19/50; 38·0%) were prevalent; whereas pathogenic phylogroups B2 and D were observed in only 10·0% (n = 5/50). Swarming and swimming motilities were observed in 90·0% (n = 45/50) and 68·0% (n = 34/50) of the isolates, respectively; and there was a significant association (P = 0·0036) between swarming motility and severe clinical cases (score 3). To the best of our knowledge, this is the first study where clinical severity of bovine mastitis cases and the genes proposed to classify ExPEC were assessed in relation to swarming and swimming motility. SIGNIFICANCE AND IMPACT OF THE STUDY: Escherichia coli is classified as extra-intestinal (ExPEC) when strains contain at least two of the genes kpsMTII, iucD, sfaDE, afaBC and papA and/or papC. We investigated in vitro motility and the presence of these genes in 50 E. coli isolated from bovine mastitis with different clinical scores (mild, moderate and severe). Clinical severity was not associated with the genes studied. Swarming motility was associated with severe cases (score 3) of clinical mastitis. Results of this study contribute to a better understanding of the factors that determine the severity of clinical mastitis.
Asunto(s)
Infecciones por Escherichia coli/veterinaria , Escherichia coli/aislamiento & purificación , Escherichia coli/patogenicidad , Locomoción/genética , Mastitis Bovina/microbiología , Animales , Bovinos , Enfermedades de los Bovinos/microbiología , Escherichia coli/genética , Proteínas de Escherichia coli/genética , Femenino , Filogenia , Factores de Virulencia/genéticaRESUMEN
Brucellosis remains as a major infectious disease of domestic animals and is considered a re-emerging zoonosis in several countries. B. abortus infections in bulls are related to reproductive tract infections, although infected animals show transient serological titers or nonreactor status. Thus, diagnosis of bovine brucellosis based exclusively on serological tests probably underestimates B. abortus infections in bulls. In this scenario, three hundred thirty-five serum samples from reproductively mature bovine bulls were subjected simultaneously to standard serodiagnosis using the rose Bengal test (RBT), 2-mercaptoethanol (2-ME), complement fixation (CFT), and fluorescence polarization assay (FPA). Furthermore, conventional semen plasma agglutination (SPA) and modified 2-ME, FC and, FPA were carried out in all bulls replaing serum by seminal plasma. Semen from all bulls was also analyzed for sperm viability, microbiological culture in Farrell media, and polymerase chain reaction (PCR). Only eight (2.38%) semen samples were considered improper for reproduction services (necrospermia and azoospermia), although none of these animals was positive in any of the diagnosis methods used. Five bulls (1.49%) were simultaneously positive in conventional RBT, 2-ME, SPA, modified 2-ME, microbiological culture in Farrell media, and in PCR for B. abortus strain 19. Two (1.67%) bulls were positive in PCR for B. abortus field strains and negative in all other tests, although semen was considered viable to reproduction service. The identification of B. abortus B19 strain in serum and semen of bulls occurred probably due to improper vaccination of males or infection by B19 strain shedding by vaccinated females that could to contaminated environment of farms. In addition, detection of B. abortus field strains only using PCR in bulls without sperm viability abnormalities indicate the need for including molecular methods to improve diagnosis of the disease in bovine bulls.
Asunto(s)
Brucella abortus , Brucelosis Bovina/sangre , Brucelosis Bovina/microbiología , Semen/microbiología , Animales , Bovinos , MasculinoRESUMEN
This report describes a fatal case of a pet dog with major enteric signs owned by a family that has experienced cases of pulmonary tuberculosis (TB) in the household. Clinical and epidemiological aspects, imaging data, microbiological, haematological and histopathological examinations were assessed to diagnosis of disease. gyrB-RFLP, spoligotyping and MIRU-VNTR allowed molecular detection of M. tuberculosis strain from S family. The resazurin microtiter assay indicated that all isolates were resistant to isoniazid, ethambutol, ciprofloxacin, ofloxacin, streptomycin and amikacin. The public health concerns related to canine tuberculosis and risk of the dissemination by pets of M. tuberculosis pre-multidrug-resistant (PMD) to isoniazid, ethambutol and other first-line drugs used in human therapy of TB are discussed. We believe this to be the first report of PMD M. tuberculosis infection in a dog presenting mainly enteric manifestation, confirmed as S lineage by molecular methods, owned by a family in which TB has spread in the household for generations.
Asunto(s)
Enfermedades de los Perros/microbiología , Farmacorresistencia Bacteriana Múltiple , Enteritis/veterinaria , Mycobacterium tuberculosis/efectos de los fármacos , Tuberculosis Pulmonar/microbiología , Animales , Antituberculosos/farmacología , Enfermedades de los Perros/diagnóstico , Perros , Enteritis/diagnóstico , Enteritis/microbiología , Resultado Fatal , Humanos , Isoniazida/farmacología , Masculino , Pruebas de Sensibilidad Microbiana , Mycobacterium tuberculosis/aislamiento & purificación , Mascotas , Tuberculosis Resistente a Múltiples MedicamentosRESUMEN
UNLABELLED: The virulence-plasmid profile of Rhodococcus equi strains isolated from Suidae and humans is similar. Recent evidence suggests that the consumption of pork products contaminated with faeces might be a potential source of R. equi infections in humans, mainly to patients with rhodococcosis without history of contact with pigs or pig farms. This study investigated the virulence-associated genes (vapA and vapB) and plasmid profiles of R. equi among the 150 samples of small intestinal content obtained from slaughtered pigs. In addition, all samples were subjected to microbiological culture in conventional sheep blood agar and CAZ-NB, TCP and TVP selective media. A total of 40 (26·7%) of the samples recovered R. equi, with two samples recovering isolates harbouring the VapB type 8 plasmid. Among the 150 pigs sampled herein, CAZ-NB was considered the best selective medium for the isolation of R. equi from faeces. Our results provide evidence that the contamination of slaughtered pig carcasses with pathogenic R. equi might occur through faeces, representing a public health concern. Furthermore, this study is the first description of R. equi strains carrying the VapB plasmid in the gut of pigs. SIGNIFICANCE AND IMPACT OF THE STUDY: Intermediately virulent (VapB) is a common plasmid-type harboured by R. equi isolated from pigs and humans with AIDS. Curiously, humans with rhodococcosis usually have no history of contact with pigs or pig farms. Virulence-plasmid profile of 40 R. equi isolated among 150 small intestine content samples from pigs revelled two carrying isolates with the VapB type-8 plasmids. Moreover, comparison of three selective culture media shows that CAZ-NB was the best. Our results provide evidence that contamination of slaughtered pig carcasses with pathogenic R. equi might occur through faeces, representing a public health concern. Furthermore, R. equi carrying VapB type-8 plasmids types are described for the first time in the gut of the pig.
Asunto(s)
Infecciones por Actinomycetales/microbiología , Proteínas Bacterianas/genética , Medios de Cultivo , Proteínas de Unión al ADN/genética , Microbiología de Alimentos , Glicoproteínas de Membrana/genética , Carne Roja/microbiología , Rhodococcus equi/aislamiento & purificación , Mataderos , Animales , Brasil , Heces/microbiología , Humanos , Intestino Delgado/microbiología , Plásmidos/genética , Plásmidos/aislamiento & purificación , Rhodococcus equi/genética , Porcinos , Enfermedades de los Porcinos/microbiología , Virulencia/genética , Factores de Virulencia/genéticaRESUMEN
BACKGROUND: Formerly, Arcanobacterium pyogenes was recently renamed Trueperella pyogenes. This opportunistic bacterium is related to miscellaneous pyogenic infections in animals. Most studies involving T. pyogenes are case reports, whereas few surveys have focused the major aspects of T. pyogenes infections involving a case series study design. OBJECTIVE: The aim of this study was to retrospectively evaluate selected epidemiological and clinical aspects, as well as the in vitro antimicrobial susceptibility pattern of 144 cases of T. pyogenes infections among domestic animals from 2002 to 2012. ANIMALS AND METHODS: T. pyogenes was isolated from different clinical specimens from cattle, goats, sheep, pigs, horses, dogs, and buffaloes. Correlations were assessed by the Chi-square or Fisher's exact tests. RESULTS: Mastitis (45.1%), abscesses (18.0%), pneumonia (11.1%), and lymphadenitis (9.0%) were the most common clinical manifestations. In addition, the organism was also isolated from other miscellaneous clinical specimens from cases of septicemia, encephalitis, pyometra, prostatitis, orchitis, seminal vesiculitis, pericarditis, and omphalitis. No statistical association was observed between T. pyogenes infections and age, gender, or season across the study. The most effective drugs against the pathogen were florfenicol (99.1%), cefoperazone (96.0%), cephalexin (95.0%), and ceftiofur (94.8%). High resistance rates were observed against trimethoprim-sulfamethoxazole (49.3%), followed by norfloxacin (10.9%) and tetracycline (9.2%). CONCLUSIONS: This study highlights the diversity of clinical manifestations and the opportunistic behavior of T. pyogenes infections in domestic animals, with predominance of mastitis, abscesses, pneumonia, and lymphadenitis. It also reinforces the importance of knowing the susceptibility profile before initiating therapy, to improve antimicrobial therapy approaches.
Asunto(s)
Infecciones por Actinomycetales/veterinaria , Búfalos/microbiología , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/microbiología , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/microbiología , Enfermedades de las Cabras/epidemiología , Enfermedades de las Cabras/microbiología , Enfermedades de las Ovejas/epidemiología , Enfermedades de las Ovejas/microbiología , Enfermedades de los Porcinos/epidemiología , Enfermedades de los Porcinos/microbiología , Infecciones por Actinomycetales/epidemiología , Animales , Animales Domésticos , Arcanobacterium/aislamiento & purificación , Brasil/epidemiología , Bovinos , Perros , Femenino , Cabras , Masculino , Mastitis Bovina/epidemiología , Mastitis Bovina/microbiología , Neumonía/epidemiología , Neumonía/microbiología , Neumonía/veterinaria , Estudios Retrospectivos , Estaciones del Año , Ovinos , PorcinosRESUMEN
The aim of this study was to evaluate somatic cell count (SCC), prevalence and etiology of mastitis in a dairy buffalo herd from Analândia, São Paulo State, Brazil, in the dry and rainy seasons. Additionally, antimicrobial susceptibility profile of microorganisms isolated from milk samples was also evaluated. 1,042 milk samples from female Murrah buffaloes in a dairy farm located in Analândia, São Paulo State, Brazil, collected between May 2011 and November 2012 were analyzed. After the mammary gland physical examination, strip cup test and California Mastitis Test (CMT) were performed. Afterwards, 50mL of milk samples from each mammary quarter were collected aseptically for SCC in automatic equipment and microbiological examination. The antimicrobial sensitivity profile to ampicillin, cefoperazone, ceftiofur, enrofloxacin, gentamicin, neomycin, oxacillin, penicillin, and sulfamethoxazole/trimethoprim was evaluated by disk diffusion method. The monthly average temperature and pluviometric index were obtained from "Centro Integrado de Informações Agrometeorológicas" (CIIAGRO) of "Instituto Agronômico de Campinas" (IAC). Milk samples with positive results in the microbiological test showed average SCC of 137,720 cells/mL in the dry period and 190,309 cells/mL in the rainy period. Although a higher number of isolated microorganisms was observed in buffalo milk samples during the rainy period (69/600) compared to the dry period (50/442), the season had no significant effect on the frequency of isolation of microorganisms. The main genera of microorganisms isolated were coagulase-negative Staphylococcus (38.4%), Streptococcus agalactiae (28.8%), and Bacillus spp. (7.56%) during the dry season and Corynebacterium sp. (23.5%), Streptococcus spp. (32.3%), and Streptococcus agalactiae (9.24%) during the rainy period. Multidrug resistance was observed in 30.1% of the isolated microorganisms.(AU)
O objetivo do presente estudo foi avaliar a contagem de células somáticas, a prevalência e a etiologia da mastite bubalina nas estações seca e chuvosa em um rebanho de bubalinos do município de Analândia, estado de São Paulo, Brasil. Adicionalmente, verificou-se o perfil de sensibilidade antimicrobiana dos micro-organismos isolados nas amostras de leite das búfalas. Foram avaliadas 1.042 amostras de leite de búfalas da raça Murrah pertencentes a uma propriedade rural localizada no município de Analândia-SP, obtidas no período de maio de 2011 e novembro de 2012. Após o exame físico da glândula mamária, foram realizados o teste da caneca de fundo escuro e o California Mastits Test (CMT); em seguida, foram colhidas, de forma asséptica, amostras de 50mL de leite de cada quarto mamário, para a contagem de células somáticas (CCS) em aparelho automático e exame microbiológico. Também, foi avaliado o perfil de sensibilidade antimicrobiana a ampicilina, cefoperazona, ceftiofur, enrofloxacina, gentamicina, neomicina, oxacilina, penicilina e sulfametoxazol/trimetoprim, pelo método de difusão em disco. A temperatura média e o índice pluviométrico mensais foram obtidos no Centro Integrado de Informações Agrometeorológicas (CIIAGRO) do Instituto Agronômico de Campinas (IAC). Notou-se que as amostras de leite com resultado positivo no exame microbiológico apresentaram CCS média de 137.720 células/mL, no período seco, e 190.309 células/mL, no período chuvoso. Embora tenha se constatado maior índice de isolamentos de micro-organismos nas amostras de leite obtidas no período chuvoso (69/600) do que no período seco (50/442), a frequência de isolamentos não foi influenciada significativamente pela estação do ano. Os principais gêneros de micro-organismos isolados durante o período seco foram Staphylococcus coagulase negativa (38,4%), Streptococcus agalactiae (28,8%) e Bacillus spp. (7,56%), e no período chuvoso Corynebacterium sp. (23,5%), Streptococcus spp. (32,3%) e Streptococcus agalactiae (9,24%). Verificou-se resistência a três ou mais antimicrobianos em 30,1% dos micro-organismos isolados.(AU)
Asunto(s)
Animales , Búfalos , Mastitis Bovina/inmunología , Glándulas Mamarias Animales , Enfermedad de la Leche/microbiología , Enfermedad de la Leche/veterinaria , Antiinfecciosos/análisisRESUMEN
The aim of this study was to evaluate somatic cell count (SCC), prevalence and etiology of mastitis in a dairy buffalo herd from Analândia, São Paulo State, Brazil, in the dry and rainy seasons. Additionally, antimicrobial susceptibility profile of microorganisms isolated from milk samples was also evaluated. 1,042 milk samples from female Murrah buffaloes in a dairy farm located in Analândia, São Paulo State, Brazil, collected between May 2011 and November 2012 were analyzed. After the mammary gland physical examination, strip cup test and California Mastitis Test (CMT) were performed. Afterwards, 50mL of milk samples from each mammary quarter were collected aseptically for SCC in automatic equipment and microbiological examination. The antimicrobial sensitivity profile to ampicillin, cefoperazone, ceftiofur, enrofloxacin, gentamicin, neomycin, oxacillin, penicillin, and sulfamethoxazole/trimethoprim was evaluated by disk diffusion method. The monthly average temperature and pluviometric index were obtained from "Centro Integrado de Informações Agrometeorológicas" (CIIAGRO) of "Instituto Agronômico de Campinas" (IAC). Milk samples with positive results in the microbiological test showed average SCC of 137,720 cells/mL in the dry period and 190,309 cells/mL in the rainy period. Although a higher number of isolated microorganisms was observed in buffalo milk samples during the rainy period (69/600) compared to the dry period (50/442), the season had no significant effect on the frequency of isolation of microorganisms. The main genera of microorganisms isolated were coagulase-negative Staphylococcus (38.4%), Streptococcus agalactiae (28.8%), and Bacillus spp. (7.56%) during the dry season and Corynebacterium sp. (23.5%), Streptococcus spp. (32.3%), and Streptococcus agalactiae (9.24%) during the rainy period. Multidrug resistance was observed in 30.1% of the isolated microorganisms...
O objetivo do presente estudo foi avaliar a contagem de células somáticas, a prevalência e a etiologia da mastite bubalina nas estações seca e chuvosa em um rebanho de bubalinos do município de Analândia, estado de São Paulo, Brasil. Adicionalmente, verificou-se o perfil de sensibilidade antimicrobiana dos micro-organismos isolados nas amostras de leite das búfalas. Foram avaliadas 1.042 amostras de leite de búfalas da raça Murrah pertencentes a uma propriedade rural localizada no município de Analândia-SP, obtidas no período de maio de 2011 e novembro de 2012. Após o exame físico da glândula mamária, foram realizados o teste da caneca de fundo escuro e o California Mastits Test (CMT); em seguida, foram colhidas, de forma asséptica, amostras de 50mL de leite de cada quarto mamário, para a contagem de células somáticas (CCS) em aparelho automático e exame microbiológico. Também, foi avaliado o perfil de sensibilidade antimicrobiana a ampicilina, cefoperazona, ceftiofur, enrofloxacina, gentamicina, neomicina, oxacilina, penicilina e sulfametoxazol/trimetoprim, pelo método de difusão em disco. A temperatura média e o índice pluviométrico mensais foram obtidos no Centro Integrado de Informações Agrometeorológicas (CIIAGRO) do Instituto Agronômico de Campinas (IAC). Notou-se que as amostras de leite com resultado positivo no exame microbiológico apresentaram CCS média de 137.720 células/mL, no período seco, e 190.309 células/mL, no período chuvoso. Embora tenha se constatado maior índice de isolamentos de micro-organismos nas amostras de leite obtidas no período chuvoso (69/600) do que no período seco (50/442), a frequência de isolamentos não foi influenciada significativamente pela estação do ano...
Asunto(s)
Animales , Búfalos , Glándulas Mamarias Animales , Mastitis Bovina/inmunología , Antiinfecciosos/análisis , Enfermedad de la Leche/microbiología , Enfermedad de la Leche/veterinariaRESUMEN
The identification of pathogens of viral (Rotavirus, Coronavirus), parasitic (Toxocara spp.) and bacterial (Escherichia coli, Salmonella spp., Rhodococcus equi) origin shed in feces, and the virulence profile of R. equi and E. coli isolates were investigated in 200 samples of sand obtained from 40 parks, located in central region of state of Sao Paulo, Brazil, using different diagnostic methods. From 200 samples analyzed, 23 (11.5%) strains of R. equi were isolated. None of the R. equi isolates showed a virulent (vapA gene) or intermediately virulent (vapB gene) profiles. Sixty-three (31.5%) strains of E. coli were identified. The following genes encoding virulence factors were identified in E. coli: eae, bfp, saa, iucD, papGI, sfa and hly. Phylogenetic classification showed that 63 E. coli isolates belonged to groups B1 (52.4%), A (25.4%) and B2 (22.2%). No E. coli serotype O157:H7 was identified. Eggs of Toxocara sp. were found in three parks and genetic material of bovine Coronavirus was identified in one sample of one park. No Salmonella spp. and Rotavirus isolates were identified in the samples of sand. The presence of R. equi, Toxocara sp, bovine Coronavirus and virulent E. coli isolates in the environment of parks indicates that the sanitary conditions of the sand should be improved in order to reduce the risks of fecal transmission of pathogens of zoonotic potential to humans in these places.
Asunto(s)
Coronavirus Bovino/aislamiento & purificación , Escherichia coli/aislamiento & purificación , Rhodococcus equi/aislamiento & purificación , Microbiología del Suelo , Toxocara/aislamiento & purificación , Animales , Brasil , Escherichia coli/clasificación , Escherichia coli/genética , Humanos , Rhodococcus equi/genética , Factores de Virulencia/genéticaRESUMEN
The identification of pathogens of viral (Rotavirus, Coronavirus), parasitic (Toxocara spp.) and bacterial (Escherichia coli, Salmonella spp., Rhodococcus equi) origin shed in feces, and the virulence profile of R. equi and E. coli isolates were investigated in 200 samples of sand obtained from 40 parks, located in central region of state of Sao Paulo, Brazil, using different diagnostic methods. From 200 samples analyzed, 23 (11.5%) strains of R. equi were isolated. None of the R. equi isolates showed a virulent (vapA gene) or intermediately virulent (vapB gene) profiles. Sixty-three (31.5%) strains of E. coli were identified. The following genes encoding virulence factors were identified in E. coli: eae, bfp, saa, iucD, papGI, sfa and hly. Phylogenetic classification showed that 63 E. coli isolates belonged to groups B1 (52.4%), A (25.4%) and B2 (22.2%). No E. coli serotype O157:H7 was identified. Eggs of Toxocara sp. were found in three parks and genetic material of bovine Coronavirus was identified in one sample of one park. No Salmonella spp. and Rotavirus isolates were identified in the samples of sand. The presence of R. equi, Toxocara sp, bovine Coronavirus and virulent E. coli isolates in the environment of parks indicates that the sanitary conditions of the sand should be improved in order to reduce the risks of fecal transmission of pathogens of zoonotic potential to humans in these places.
Asunto(s)
Animales , Humanos , Coronavirus Bovino/aislamiento & purificación , Escherichia coli/aislamiento & purificación , Rhodococcus equi/aislamiento & purificación , Microbiología del Suelo , Toxocara/aislamiento & purificación , Brasil , Escherichia coli/clasificación , Escherichia coli/genética , Rhodococcus equi/genética , Factores de Virulencia/genéticaRESUMEN
The identification of pathogens of viral (Rotavirus, Coronavirus), parasitic (Toxocara spp.) and bacterial (Escherichia coli, Salmonella spp., Rhodococcus equi) origin shed in feces, and the virulence profile of R. equi and E. coli isolates were investigated in 200 samples of sand obtained from 40 parks, located in central region of state of Sao Paulo, Brazil, using different diagnostic methods. From 200 samples analyzed, 23 (11.5%) strains of R. equi were isolated. None of the R. equi isolates showed a virulent (vapA gene) or intermediately virulent (vapB gene) profiles. Sixty-three (31.5%) strains of E. coli were identified. The following genes encoding virulence factors were identified in E. coli: eae, bfp, saa, iucD, papGI, sfa and hly. Phylogenetic classification showed that 63 E. coli isolates belonged to groups B1 (52.4%), A (25.4%) and B2 (22.2%). No E. coli serotype O157:H7 was identified. Eggs of Toxocara sp. were found in three parks and genetic material of bovine Coronavirus was identified in one sample of one park. No Salmonella spp. and Rotavirus isolates were identified in the samples of sand. The presence of R. equi, Toxocara sp, bovine Coronavirus and virulent E. coli isolates in the environment of parks indicates that the sanitary conditions of the sand should be improved in order to reduce the risks of fecal transmission of pathogens of zoonotic potential to humans in these places.(AU)
Asunto(s)
Rhodococcus , Humanos , Toxocara , Escherichia coli , Factores de VirulenciaRESUMEN
Two strains of Nocardia spp. were isolated from bovine milk of two individual bulk tank. Molecular identification classified the strains as Nocardia farcinica and Nocardia cyriacigeorgica. The thermorresistance to boiling of the isolates was carried out and was observed bacterial growth after boiling. Our findings indicate the potential risk of pathogen transmission to humans through contaminated milk with Nocardia spp.
Asunto(s)
Bovinos , Crecimiento Bacteriano , Sustitutos de la Leche Humana , Conservación de Alimentos , Mastitis Bovina , Nocardia/aislamiento & purificación , Streptococcus bovis/aislamiento & purificación , Bovinos , Muestras de Alimentos , Métodos , Leche , VirulenciaRESUMEN
Two strains of Nocardia spp. were isolated from bovine milk of two individual bulk tank. Molecular identification classified the strains as Nocardia farcinica and Nocardia cyriacigeorgica. The thermorresistance to boiling of the isolates was carried out and was observed bacterial growth after boiling. Our findings indicate the potential risk of pathogen transmission to humans through contaminated milk with Nocardia spp.
RESUMEN
O agente primário da actinomicose/osteomielite mandibular é o Actinomyces bovis que infecta principalmente ossos da cavidade oral de bovinos, acarretando prejuízos econômicos devido a perda de peso ocasionada pela dor à mastigação. O diagnóstico é comumente realizado por um bom exame clínico, radiográfico, bacterioscopia e cultivo microbiológico. Neste relato é apresentado o caso de um ovino com aumento localizado de mandíbula. O exame radiográfico demonstrou osteomielite. Amostras da lesão foram coletadas para realização de exame microbiológico e histopatológico. A histopatologia evidenciou lesão piogranulomatosa e na bacterioscopia foi demonstrado um agente Gram-positivo ramificado, filamentoso e catalase negativo. Os achados clínicos associados aos resultados dos exames complementares confirmaram a ocorrência incomum de infecção mandibular por A. bovis em um ovino.
JAW ACTINOMYCOSIS IN A SHEEP: CASE REPORT. The primary agent of actinomicosis/mandibular osteomyelitis is Actinomyces bovis that infects mainly bones of the oral cavity of cattle, resulting in economic losses due to weight loss caused by pain on chewing. The diagnosis is usually made by a good clinical examination, radiographic, bacterioscopy and microbiological culture. This report describes the case of a sheep that had localized increase of the jaw, where the material from the lesion was collected for microbiological and histopathological diagnosis. Osteomyelitis was demonstrated by radiography. Piogranuloma was observed at the histophatology. Bacterioscopy evidenced a Gram-positive, branched, filamentous and catalase negative agent. The main clinical findings and diagnoses confirmed the occurrence of unusual jaw infection by A. bovis in a sheep.
Asunto(s)
Actinomicosis/patología , Actinomyces , Mandíbula/anatomía & histología , Ovinos/clasificación , RadiografíaRESUMEN
O presente estudo avaliou a composição química, a contagem bacteriana total, o nível de celularidade e presença de resíduos de antimicrobianos no leite de duas propriedades em sistema orgânico de produção do interior do Estado São Paulo com base na IN 062-MAPA. O leite da Propriedade I apresentou média de CCS de 7,13 x 105 céls/mL e o da Propriedade II 3,27 x 105 céls/mL. As médias das CBTs foram de 7,80 x 105 céls/mL na Propriedade I e de 6,15 x 105 céls/mL na Propriedade II. A propriedade I apresentou como média de gordura de 3,10%, de proteína 3,26% e 4,54% de lactose, enquanto a Propriedade II apresentou 3,44% de gordura, 3,39% de proteína e 4,50% de lactose, nos 12 meses avaliados. A presença de resíduos de antimicrobianos foi detectada em seis amostras (6,97%) da Propriedade I e em 16 (9,24%) da Propriedade II. Os resultados obtidos revelam falha nos preceitos da produção orgânica.
QUALITY INDICATORS OF ORGANIC BOVINE MILK IN TWO CERTIFIED DAIRY HERDS IN SÃO PAULO STATE, BRAZIL. This study evaluated the chemical composition, total bacterial count, the level of cellularity and presence of antimicrobial residues in milk from two small farms in organic production system in the state of São Paulo, Brazil, based in NI 062-MAPA. The milk of the Property I had the mean of CCS 7.13 x 105 cells/mL and the Property II had 3.27 x 105 cells/mL. The averages of CBT were, respectively, 7.80 x 105 cells/mL in the Property I and 6.15 x 105cells/mL in Property II. The Property I presented as an average of 3.10% of fat, 3.26% of protein and 4.54% of lactose, while the Property II presented 3.44% of fat, 3.39% of protein and 4.50% of lactose in 12 months assessed. The presence of residues of antimicrobial drugs was detected in six samples (6.97%) of the Property I and 16 (9.24%) of Property II. The results obtained reveal failure in the percepts of organic production.
Asunto(s)
Proteínas/química , Calidad de los Alimentos , Sustitutos de la Leche Humana , Bovinos/clasificación , Leche/clasificaciónRESUMEN
O presente estudo avaliou a composição química, a contagem bacteriana total, o nível de celularidade e presença de resíduos de antimicrobianos no leite de duas propriedades em sistema orgânico de produção do interior do Estado São Paulo com base na IN 062-MAPA. O leite da Propriedade I apresentou média de CCS de 7,13 x 105 céls/mL e o da Propriedade II 3,27 x 105 céls/mL. As médias das CBTs foram de 7,80 x 105 céls/mL na Propriedade I e de 6,15 x 105 céls/mL na Propriedade II. A propriedade I apresentou como média de gordura de 3,10%, de proteína 3,26% e 4,54% de lactose, enquanto a Propriedade II apresentou 3,44% de gordura, 3,39% de proteína e 4,50% de lactose, nos 12 meses avaliados. A presença de resíduos de antimicrobianos foi detectada em seis amostras (6,97%) da Propriedade I e em 16 (9,24%) da Propriedade II. Os resultados obtidos revelam falha nos preceitos da produção orgânica.(AU)
QUALITY INDICATORS OF ORGANIC BOVINE MILK IN TWO CERTIFIED DAIRY HERDS IN SÃO PAULO STATE, BRAZIL. This study evaluated the chemical composition, total bacterial count, the level of cellularity and presence of antimicrobial residues in milk from two small farms in organic production system in the state of São Paulo, Brazil, based in NI 062-MAPA. The milk of the Property I had the mean of CCS 7.13 x 105 cells/mL and the Property II had 3.27 x 105 cells/mL. The averages of CBT were, respectively, 7.80 x 105 cells/mL in the Property I and 6.15 x 105cells/mL in Property II. The Property I presented as an average of 3.10% of fat, 3.26% of protein and 4.54% of lactose, while the Property II presented 3.44% of fat, 3.39% of protein and 4.50% of lactose in 12 months assessed. The presence of residues of antimicrobial drugs was detected in six samples (6.97%) of the Property I and 16 (9.24%) of Property II. The results obtained reveal failure in the percepts of organic production.(AU)
Asunto(s)
Sustitutos de la Leche Humana , Proteínas/química , Calidad de los Alimentos , /patología , Bovinos/clasificación , Leche/clasificaciónRESUMEN
O agente primário da actinomicose/osteomielite mandibular é o Actinomyces bovis que infecta principalmente ossos da cavidade oral de bovinos, acarretando prejuízos econômicos devido a perda de peso ocasionada pela dor à mastigação. O diagnóstico é comumente realizado por um bom exame clínico, radiográfico, bacterioscopia e cultivo microbiológico. Neste relato é apresentado o caso de um ovino com aumento localizado de mandíbula. O exame radiográfico demonstrou osteomielite. Amostras da lesão foram coletadas para realização de exame microbiológico e histopatológico. A histopatologia evidenciou lesão piogranulomatosa e na bacterioscopia foi demonstrado um agente Gram-positivo ramificado, filamentoso e catalase negativo. Os achados clínicos associados aos resultados dos exames complementares confirmaram a ocorrência incomum de infecção mandibular por A. bovis em um ovino. (AU)
JAW ACTINOMYCOSIS IN A SHEEP: CASE REPORT. The primary agent of actinomicosis/mandibular osteomyelitis is Actinomyces bovis that infects mainly bones of the oral cavity of cattle, resulting in economic losses due to weight loss caused by pain on chewing. The diagnosis is usually made by a good clinical examination, radiographic, bacterioscopy and microbiological culture. This report describes the case of a sheep that had localized increase of the jaw, where the material from the lesion was collected for microbiological and histopathological diagnosis. Osteomyelitis was demonstrated by radiography. Piogranuloma was observed at the histophatology. Bacterioscopy evidenced a Gram-positive, branched, filamentous and catalase negative agent. The main clinical findings and diagnoses confirmed the occurrence of unusual jaw infection by A. bovis in a sheep. (AU)
Asunto(s)
Actinomicosis/patología , Actinomyces , Mandíbula/anatomía & histología , Ovinos/clasificación , RadiografíaRESUMEN
Two strains of Nocardia spp. were isolated from bovine milk of two individual bulk tank. Molecular identification classified the strains as Nocardia farcinica and Nocardia cyriacigeorgica. The thermorresistance to boiling of the isolates was carried out and was observed bacterial growth after boiling. Our findings indicate the potential risk of pathogen transmission to humans through contaminated milk with Nocardia spp.
RESUMEN
This study evaluated the chemical composition, total bacterial count, the level of cellularity and presence of antimicrobial residues in milk from two small farms in organic production system in the state of São Paulo, Brazil, based in NI 062-MAPA. The milk of the Property I had the mean of CCS 7.13 x 10(5) cells/mL and the Property II had 3.27 x 10(5) cells/mL. The averages of CBT were, respectively, 7.80 x 10(5) cells/mL in the Property I and 6.15 x 10(5) cells/mL in Property II. The Property I presented as an average of 3.10% of fat, 3.26% of protein and 4.54% of lactose, while the Property II presented 3.44% of fat, 3.39% of protein and 4.50% of lactose in 12 months assessed. The presence of residues of antimicrobial drugs was detected in six samples (6.97%) of the Property I and 16 (9.24%) of Property II. The results obtained reveal failure in the percepts of organic production.
O presente estudo avaliou a composição química, a contagem bacteriana total, o nível de celularidade e presença de resíduos de antimicrobianos no leite de duas propriedades em sistema orgânico de produção do interior do Estado São Paulo com base na IN 062-MAPA. O leite da Propriedade I apresentou média de CCS de 7,13 x 10(5) céls/mL e o da Propriedade II 3,27 x 10(5) céls/mL. As médias das CBTs foram de 7,80 x 10(5) céls/mL na Propriedade I e de 6,15 x 10(5) céls/mL na Propriedade II. A propriedade I apresentou como média de gordura de 3,10%, de proteína 3,26% e 4,54% de lactose, enquanto a Propriedade II apresentou 3,44% de gordura, 3,39% de proteína e 4,50% de lactose, nos 12 meses avaliados. A presença de resíduos de antimicrobianos foi detectada em seis amostras (6,97%) da Propriedade I e em 16 (9,24%) da Propriedade II. Os resultados obtidos revelam falha nos preceitos da produção orgânica.
RESUMEN
The primary agent of actinomicosis/mandibular osteomyelitis is Actinomyces bovis that infects mainly bones of the oral cavity of cattle, resulting in economic losses due to weight loss caused by pain on chewing. The diagnosis is usually made by a good clinical examination, radiographic, bacterioscopy and microbiological culture. This report describes the case of a sheep that had localized increase of the jaw, where the material from the lesion was collected for microbiological and histopathological diagnosis. Osteomyelitis was demonstrated by radiography. Piogranuloma was observed at the histophatology. Bacterioscopy evidenced a Gram-positive, branched, filamentous and catalase negative agent. The main clinical findings and diagnoses confirmed the occurrence of unusual jaw infection by A. bovis in a sheep.
O agente primário da actinomicose/osteomielite mandibular é o Actinomyces bovis que infecta principalmente ossos da cavidade oral de bovinos, acarretando prejuízos econômicos devido a perda de peso ocasionada pela dor à mastigação. O diagnóstico é comumente realizado por um bom exame clínico, radiográfico, bacterioscopia e cultivo microbiológico. Neste relato é apresentado o caso de um ovino com aumento localizado de mandíbula. O exame radiográfico demonstrou osteomielite. Amostras da lesão foram coletadas para realização de exame microbiológico e histopatológico. A histopatologia evidenciou lesão piogranulomatosa e na bacterioscopia foi demonstrado um agente Gram-positivo ramificado, filamentoso e catalase negativo. Os achados clínicos associados aos resultados dos exames complementares confirmaram a ocorrência incomum de infecção mandibular por A. bovis em um ovino.
RESUMEN
Routine diagnosis methods used in bovine mastitis were studied in 55 mares in lactation. The findings of strip cup test, California Mastitis Test-CMT, electronic somatic cell count-CCS, microbiological culture, and in vitro antimicrobial susceptibility profile of isolates were discussed. Streptococcus spp., Staphylococcus spp, and enterobacteria were the most common microorganisms isolated in health and CMT-positive mammary glands. Staphylococcus aureus and Arcanobacterium pyogenes were identified in two mares presenting clinical mastitis. Mean somatic cell count of eight mares without presence of microorganisms in milk was 247.57x10³/mL and 1.621,86x10³/mL in 47 mares with positive microbiological culture. Moderate concordance (63.8 percent) between positive reactions in CMT (1 to 3+) and microbiological culture was observed. Amicacin (78.9 percent), ceftiofur (74.7 percent), sulpha-trimetoprim (69,0 percent) and norfloxacin (69.0 percent), were the most effective drugs, while resistance of isolates was mainly observed against penicillin (64.8 percent), gentamycin (35.2 percent), azithromycin (35.2 percent), enrofloxacin (28.2 percent), and florfenicol (28.2 percent).