RESUMEN
The name of the species Gordonia nitida is validly published but its type strain DSM 44499(T) shares high similarity based on 16S rRNA gene sequences with Gordonia alkanivorans DSM 44369(T) and Gordonia westfalica DSM 44215(T). These three species obviously build up a distinct cluster within the genus Gordonia. In the present paper, data from the literature concerning the three Gordonia species were reviewed and the genetic similarity of G. nitida DSM 44499(T) and G. alkanivorans DSM 44369(T) was further investigated by DNA-DNA-hybridization experiments, revealing approximately 80 % DNA-DNA relatedness. Even though the two type strains could be differentiated by automated ribotyping, it is proposed that, according to the rules of priority, G. nitida is a later synonym of G. alkanivorans.
Asunto(s)
ADN Bacteriano/genética , Bacteria Gordonia/clasificación , Bacteria Gordonia/genética , ADN Bacteriano/análisis , Desoxirribonucleasas de Localización Especificada Tipo II , Hibridación de Ácido Nucleico , Filogenia , Ribotipificación , Especificidad de la EspecieRESUMEN
A cis-1,4-polyisoprene-degrading bacterium (strain Kb2T) was isolated from foul water taken from the inside of a deteriorated automobile tyre found on a farmer's field in Westfalia, Germany. The strain was aerobic, Gram-positive, exhibited orange smooth and rough colonies on complex nutrient agar, produced elementary branching hyphae that fragmented into rod/coccus-like elements and showed chemotaxonomic markers which were consistent with its classification within the genus Gordonia, i.e. the presence of mesodiaminopimelic acid, arabinose and galactose in whole-cell hydrolysates (cell-wall chemotype IV), N-glycolylmuramic acid in the peptidoglycan wall, a fatty-acid pattern composed of unbranched saturated and monounsaturated fatty acids plus tuberculostearic acid, mycolic acids comprising 56-60 carbon atoms and MK-9(H2) as the only menaquinone. The 16S rDNA sequence of strain Kb2T was found to be most similar to the 16S rDNA sequences of the type strains of Gordonia alkanivorans (DSM 44369T) and Gordonia nitida (KCTC 0605BPT). However, DNA-DNA relatedness data showed that strain Kb2T ( =DSM 44215T NRRL B-24152T) could be distinguished from these two species and represented a new species within the genus Gordonia, for which the name Gordonia westfalica is proposed.