RESUMEN
Fungal ß-glucans have received a lot of interest due to their proinflammatory activity towards cells of the innate immune system. Although commonly described as (1â3)-ß-glucans with varying degree of (1â6)-branching, the fungal ß-glucans constitute a diverse polysaccharide class. In this study, the alkali-soluble ß-glucans from the edible mushroom Pleurotus eryngii were extracted and characterized by GC, GC-MS and 2D NMR analyses. The extracts contain several structurally different polysaccharides, including a (1â3)-ß-d-glucan with single glucose units attached at O-6, and a (1â6)-ß-d-glucan, possibly branched at O-3. The immunomodulatory activities of the P. eryngii extracts were assessed by investigating their ability to bind to the receptor dectin-1, and their ability to induce production of the proinflammatory cytokines TNF-α, IL-6 and IL-1ß in LPS-differentiated THP-1 cells. Although the samples were able to bind to the dectin-1a receptor, they did not induce production of significant levels of cytokines in the THP-1 cells. Positive controls of yeast-derived (1â3)-ß-d-glucans with branches at O-6 induced cytokine production in the cells. Thus, it appears that the P. eryngii ß-glucans are unable to induce production of proinflammatory cytokines in LPS-differentiated THP-1 cells, despite being able to activate the human dectin-1a receptor.