RESUMEN
Surface Ia-positive cells were found to vary from 0 to 100% in initial blood specimens from 37 adults with acute myelogenous leukemia (AML). When myeloblasts from 19 patients were tested against panels of lymphocytes from 5 to 19 normal donors, mean stimulation indices ranged from 1 to 60. Some leukemic myeloblasts strongly stimulated most allogenic responder lymphocytes whereas others produced almost no stimulation. The addition of antibody against human Ia to 28 mixed leukocyte reaction (MLR) combinations resulted in significant inhibition (p less than 0.001) of 3H-thymidine incorporation. Testing of myeloblastic Ia may have clinical relevance because patients with greater than 50% Ia-positive myeloblasts had a significantly longer survival than patients with fewer Ia-positive myeloblasts (p less than 0.04).
Asunto(s)
Antígenos de Superficie/análisis , Antígenos de Histocompatibilidad Clase II/análisis , Leucemia Mieloide Aguda/inmunología , Leucocitos/inmunología , Prueba de Cultivo Mixto de Linfocitos , Adulto , Pruebas Inmunológicas de Citotoxicidad , Antígenos de Histocompatibilidad Clase II/inmunología , Humanos , Leucemia Mieloide Aguda/diagnóstico , PronósticoRESUMEN
HLA-A, -B, -C and -D antigens were determined in 54 male Myasthenia Gravis patients. A significant increase of the B8 antigen frequency was found among patients with an onset of the disease before the age of 35. No significant increase was found among the D antigens. In a combined male and female material with an early age at onset, thymic hyperplasia was correlated to the presence of HLA-B8.
Asunto(s)
Antígenos HLA , Miastenia Gravis/inmunología , Adulto , Envejecimiento , Femenino , Humanos , Masculino , FenotipoRESUMEN
A rabbit antiserum has been raised against highly purified, detergent-solubilized HLA-DR antigens. Externally or internally labelled surface glycoproteins from B-lymphocytes, epidermis, macrophages, and B-lymphoma cell lines reacted with the antiserum which precipitated polypeptide chains with the molecular weights 28,000 and 34,000. Such polypeptide chains were not observed when the antiserum was reacted with T-lymphocytes, T-cell lymphoma lines, kidney, brain, thymus and spermatozoa. Fab-fragments of the antiserum completely abolished the cytotoxic action of HLA-DR allantisera but had no effect on the cytotoxicity mediated by HLA-A,B and C loci antisera. Moreover, the rabbit antiserum reacted with the same molecules as the HLA-DR alloantisera. Fab-fragments of the rabbit antiserum completely abolished the MLR response and from the kinetics of the inhibition it is concluded that the Fab-fragments may interfere primarily with the initial recognition phase of the MLR.
Asunto(s)
Antígenos HLA , Sueros Inmunes , Animales , Línea Celular , Membrana Celular/inmunología , Epítopos , Antígenos HLA/aislamiento & purificación , Humanos , Fragmentos Fab de Inmunoglobulinas , Inmunoglobulina G , Técnicas Inmunológicas , Prueba de Cultivo Mixto de Linfocitos , Linfocitos/inmunología , Macrófagos/inmunología , Conejos , RadioinmunoensayoAsunto(s)
Fibrosis Quística/inmunología , Antígenos HLA , Adolescente , Adulto , Enfermedad Celíaca/inmunología , Niño , Preescolar , Femenino , Frecuencia de los Genes , Antígenos HLA/análisis , Humanos , Lactante , MasculinoRESUMEN
Sixty-two patients with Crohn's disease and 51 with ulcerative colitis were HL-A typed. Phenotype frequencies were calculated and compared with those of a control group of 335 individuals. A higher frequency of HL-A17 was found among the Crohn's disease patient but this was not significant. However, HL-A17 has been included in two studies on Crohn's disease and the combined relative risk is 3.24 which significantly deviates from 1 unity. No statistically significant differences in the HL-A antigen distribution were found among the patients with ulcerative colitis.
Asunto(s)
Colitis Ulcerosa/inmunología , Enfermedad de Crohn/inmunología , Antígenos HLA , Antígenos de Histocompatibilidad , Adulto , Colitis Ulcerosa/genética , Enfermedad de Crohn/genética , Femenino , Frecuencia de los Genes , Antígenos HLA/análisis , Antígenos de Histocompatibilidad/análisis , Humanos , Masculino , FenotipoRESUMEN
Intact antibodies, and F(ab')2, and Fab' fragments thereof directed against beta2-microglobulin (beta2m) and the HLA alloantigenic polypeptide chain impede the mixed leukocyte culture reaction (MLR). The Fab' fragments are effective only when bound to the responder cell population. The kinetics of their inhibiting action suggest that they interfere with the recognition phase of the MLR. Thus, it seems possible that beta2m may be part of or closely associated with those structures on the responding cells which recognize the stimulatory alloantigens. Antibodies against beta2m or the other HLA antigen polypeptide chain are mitogenic for peripheral blood lymphocytes. The antibodies against beta2m caused greater stimulation than those against the HLA alloantigenic polypeptide chain. Only bivalent antibodies against beta2m were mitogenic and promoted blast transformation of 1 to 2% of the lymphocytes. Although only B lymphocytes were triggered, the mitogenic response of cultures containing various proportions of B and T cells suggests that cooperation of the two cell types is necessary to obtain optimal mitogenic stimulation.
Asunto(s)
beta-Globulinas/inmunología , Antígenos HLA , Antígenos de Histocompatibilidad , Inmunidad Celular , Microglobulina beta-2/inmunología , Reacciones Antígeno-Anticuerpo , Linfocitos B/inmunología , Humanos , Fragmentos Fab de Inmunoglobulinas , Inmunoglobulina G/metabolismo , Activación de Linfocitos , Prueba de Cultivo Mixto de Linfocitos , Mitógenos , Linfocitos T/inmunología , Factores de TiempoRESUMEN
HLA antigens are coded for by three closely linked loci. The gene products of the first and second locus are known to be very similar in overall structure. Antigens coded for by the third locus were examined with regard to their chemical and immunological relationship to the first and second loci gene products. It is demonstrated that the third locus antigens are composed of two types of polypeptide chains, the smaller of which is identical to beta2-microglobulin. The alloantigenic polypeptide chain has an apparent molecular weight of 48 000 when solubilized by detergen treatment and about 35 000 when released from the cell membrane by proteolysis. These data together with size and shape analyses by gel chromatography and sedimentation velocity determinations show that HLA antigens from the three subloci are indistinguishable with the techniques employed. Suggestive evidence was obtained that the third locus antigens are more sensitive to proteolysis than the first and second locus antigens. The similarity between the three gene products was also evident on cyanogen bromide fragmentation of the polypeptide chains. Furthermore, rabbit antibodies against antigens coded for by the first locus cross-reacted extensively with antigens from the second and third locus. This study therefore lends strong credence to the view that the three HLA antigen subloci have arisen by gene duplications of a common ancestral gene.
Asunto(s)
Genes , Antígenos HLA , Antígenos de Histocompatibilidad , Reacciones Cruzadas , Bromuro de Cianógeno , Detergentes , Antígenos HLA/análisis , Antígenos de Histocompatibilidad/análisis , Humanos , Peso Molecular , Papaína , Biosíntesis de Proteínas , Solubilidad , Microglobulina beta-2/análisisAsunto(s)
Antígenos de Histocompatibilidad/análisis , Isoantígenos/análisis , Animales , Complejo Antígeno-Anticuerpo , Membrana Celular/análisis , Cromatografía en Gel , Electroforesis en Gel de Poliacrilamida , Antígenos de Histocompatibilidad/metabolismo , Humanos , Radioisótopos de Yodo , Isoantígenos/metabolismo , Ratones , Péptidos/análisisAsunto(s)
beta-Globulinas , Membrana Celular/inmunología , Epítopos , Globulinas , Antígenos de Histocompatibilidad/análisis , Linfocitos/inmunología , Secuencia de Aminoácidos , Animales , Formación de Anticuerpos , Especificidad de Anticuerpos , Reacciones Antígeno-Anticuerpo , Sitios de Unión de Anticuerpos , Pruebas Inmunológicas de Citotoxicidad , Fluoresceínas , Cabras/inmunología , Prueba de Histocompatibilidad , Humanos , Fragmentos Fab de Inmunoglobulinas , Isoantígenos , Conejos/inmunología , TiocianatosAsunto(s)
beta-Globulinas/análisis , Membrana Celular/inmunología , Antígenos de Histocompatibilidad , Prueba de Histocompatibilidad , Activación de Linfocitos , Animales , Anticuerpos , Sitios de Unión de Anticuerpos , Pruebas Inmunológicas de Citotoxicidad , Depresión Química , Relación Dosis-Respuesta a Droga , Femenino , Humanos , Sueros Inmunes , Fragmentos Fab de Inmunoglobulinas/administración & dosificación , Fragmentos Fab de Inmunoglobulinas/farmacología , Fragmentos de Inmunoglobulinas , Radioisótopos de Yodo , Lectinas/farmacología , Prueba de Cultivo Mixto de Linfocitos , Linfocitos/inmunología , Métodos , Fenotipo , Conejos/inmunología , TritioRESUMEN
HL-A antigens comprising 11 different antigenic specificities were isolated after papain solubilization of spleen-cell membrane constituents. During the entire purification procedure, beta(2)-microglobulin appeared together with the HL-A antigens. The highly purified antigens were composed of two polypeptide chains. The large subunit carried the antigenic specificity whereas the small polypeptide chain was very similar, if not identical, to beta(2)-microglobulin. The two HL-A antigen polypeptide chains were held together by noncovalent interactions only, and beta(2)-microglobulin, isolated from urine, could replace the small subunit in forming a complex with the large polypeptide chain. The topographical relationship in the cell membrane between beta(2)-microglobulin and the large HL-A antigen polypeptide chain is unknown. The two polypeptide chains may be fortuitously bound as a result of the solubilization procedure.