RESUMEN
Structured Illumination Microscopy enables live imaging with sub-diffraction resolution. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the combination of Adaptive Optics and Structured Illumination Microscopy enabling imaging with 150 nm lateral and 570 nm axial resolution at a depth of 80 µm through Caenorhabditis elegans. We demonstrate that Adaptive Optics improves the three-dimensional resolution, especially along the axial direction, and reduces artifacts, successfully realizing 3D-Structured Illumination Microscopy in a variety of biological samples.
Asunto(s)
Imagenología Tridimensional/métodos , Microscopía Intravital/métodos , Iluminación/instrumentación , Animales , Artefactos , Ascomicetos , Caenorhabditis elegans , Línea Celular , Imagenología Tridimensional/instrumentación , Microscopía Intravital/instrumentación , Ratones , Microscopía Confocal/instrumentación , Microscopía Confocal/métodos , Microscopía Fluorescente/instrumentación , Microscopía Fluorescente/métodos , Oryza/microbiología , Reproducibilidad de los ResultadosRESUMEN
Biomimetic photonics extract the good design of nature and mimic it with photonics. The weakly electric fish genus, Eigenmannia, has a unique neural algorithm - jamming avoidance response, to facilitate their survival in the deep dark ocean, by automatically adjusts the local transmitter carrier frequency to move away from the jamming frequency when it is within the jamming spectral range. Examining our own wireless microwave systems, the situation of inadvertent jamming is very similar as that in Eigenmannia. In this article, a biomimetic photonic approach inspired by the jamming avoidance response in a weakly electric fish genus, Eigenmannia, is naturally adopted to experimentally tackle signal jamming in wireless systems. Mimicking the system with photonics enables the proposed scheme to work for frequencies from hundreds of MHz to tens of GHz.