RESUMEN
OBJECTIVE: To detect breast cancer specific gene 1 (BCSG1) expression in different breast tissue, analysis its correlation with clinical parameters and evaluate the prognosis of breast cancer. METHODS: The expression of BCSG1 was detected by reverse transcription-polymerase chain reaction (RT-PCR) in surgical specimens from 84 cases of breast disease patients selected randomly at XinHua Hospital affiliated with Shanghai Second Medical University from September 1999 to December 2002. Of 84 cases, 72 case were breast cancer. Statistic analysis BCSG1 gene expression correlation with clinical parameters of breast cancer. 72 breast cancers were followed up (4 - 43 months) to set up independent prognosis factor by survival analysis. RESULTS: BCSG1 was undetectable in all benign breast lesions, while was detectable in 36.1% of all breast cancer samples (26/72), in which 79.2% of stage III/IV cases were positive (19/24). The expression of BCSG1 was tightly correlated with the stage (P = 0.000) and the size of tumor (P = 0.007). Both ER (P = 0.027) and BCSG1 (P = 0.001) were the independent prognosis factor of breast cancer. CONCLUSION: BCSG1 is one of independent tumor marker of breast cancer, the expression of BCSG1 is closely correlated to the stage of breast cancer and the tumor size. Maybe, BCSG1 is a new prognosis factor of breast cancer.
Asunto(s)
Neoplasias de la Mama/genética , Proteínas de Neoplasias/genética , gamma-Sinucleína/genética , Adulto , Anciano , Anciano de 80 o más Años , Neoplasias de la Mama/diagnóstico , Neoplasias de la Mama/patología , Femenino , Expresión Génica , Humanos , Persona de Mediana Edad , Estadificación de Neoplasias , Pronóstico , ARN Mensajero/genética , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Expression of myf-5, a key component of myogenic regulatory genes, expands into the ventral marginal zone during Xenopus gastrulation after the dorsal activation takes place. Little is known about how this dynamic expression pattern occurs. Here, we provide evidences to suggest that Activin/Nodal signals participate in the regulation of ventral expression of Xmyf-5 in gastrula embryos. Two Smad binding elements (SBEs) within the Xenopus myf-5 promoter can specifically interact with Smad4 protein. Furthermore, we demonstrate that the two SBEs are both indispensable for conferring responsiveness to Activin/Nodal signals and to ventral expression of myf-5 in Xenopus gastrula embryos.
Asunto(s)
Activinas/fisiología , Proteínas de Unión al ADN , Gástrula/metabolismo , Regulación del Desarrollo de la Expresión Génica/fisiología , Proteínas Musculares/genética , Transactivadores , Factor de Crecimiento Transformador beta/fisiología , Xenopus/embriología , Animales , Secuencia de Bases , Cartilla de ADN , Femenino , Factor 5 Regulador Miogénico , Proteína Nodal , Regiones Promotoras Genéticas , Proteínas de XenopusRESUMEN
Though the Wnt/beta-catenin signaling pathway is known to play key roles during Xenopus axis specification, whether it signals exclusively through Lef/Tcf transcription factors in this process remains unclear. To investigate this issue, we generated transgenic frog embryos expressing green fluorescent protein (GFP) driven by a Lef/Tcf-dependent and Wnt/beta-catenin-responsive promoter. This promoter is highly sensitive and even detects maternal beta-catenin activity prior to the large-scale transcription of zygotic genes. Unexpectedly, GFP expression was observed only in some, but not all, known Wnt/beta-catenin-positive territories in Xenopus early development. Furthermore, ubiquitous expression of dominant Lef-1 protein variants from transgenes revealed that zygotic Lef/Tcf activity is required for the ventroposterior development of Xenopus embryos. In summary, our results suggest that endogenous Wnt/beta-catenin activity does not result in obligatory Lef/Tcf-dependent gene activation, and that the ventroposteriorizing activity of zygotic Wnt-8 signaling is mediated by Lef/Tcf proteins.
Asunto(s)
Tipificación del Cuerpo/genética , Proteínas del Citoesqueleto/genética , Proteínas de Unión al ADN/genética , Regulación del Desarrollo de la Expresión Génica , Proteínas HMGB/genética , Proteínas del Grupo de Alta Movilidad/genética , Proteínas Proto-Oncogénicas/genética , Transactivadores/genética , Factores de Transcripción/genética , Xenopus/embriología , Xenopus/genética , Proteínas de Pez Cebra , Animales , Animales Modificados Genéticamente , Cadherinas/genética , Embrión no Mamífero/fisiología , Genes Reporteros , Genes fos , Proteínas Fluorescentes Verdes , Leucina Zippers , Luciferasas/genética , Proteínas Luminiscentes/genética , Factor de Unión 1 al Potenciador Linfoide , Factor 1 Relacionado con NF-E2 , Proteínas Tirosina Quinasas/genética , Transducción de Señal , Factores de Transcripción TCF , Proteína 1 Similar al Factor de Transcripción 7 , Proteína 2 Similar al Factor de Transcripción 7 , Activación Transcripcional , Proteínas Wnt , Proteínas de Xenopus/genética , Cigoto/fisiología , beta CateninaRESUMEN
Myf-5, a member of the muscle regulatory factor family of transcription factors, plays an important role in the determination, development, and differentiation of the skeletal muscle. Factors that regulate the expression of myf-5 itself are not well understood. We show here that a T-box binding site in the Xenopus myf-5 promoter mediated the activation of myf-5 expression through specific interaction with nuclear proteins of gastrula embryos. The T-box binding site could be bound by and respond to T-box proteins. T-box genes could induce Xmyf-5. The results suggest that T-box proteins are involved in the specification of myogenic mesoderm and muscle development.