RESUMEN
Various means for changing the aggregative stability of emulsions in the system of benzylpenicillin fermentation broth filtrate and butyl acetate were tested. It was shown that the use of desemulgators was more efficient than the use of some means for removing the desemulgating admixtures. Moreover, addition of surface active substances after the preliminary thermocoagulation resulted in maximum decreasing of the emulsion aggregative stability.
Asunto(s)
Penicilina G/aislamiento & purificación , Acetatos/farmacología , Medios de Cultivo , Estabilidad de Medicamentos , Emulsiones , Concentración de Iones de Hidrógeno , Técnicas In Vitro , Penicilina G/administración & dosificación , Soluciones , Solventes/farmacología , Temperatura , Ultrafiltración/métodosRESUMEN
Inactivation of benzylpenicillin in real media i.e. fermentation broths and their filtrates was studied in comparison with the published data on inactivation of commercial benzylpenicillin in aqueous solutions as dependent on the medium pH and temperature. The lowest constant of benzylpenicillin inactivation was shown to be in the fermentation broths.
Asunto(s)
Penicilina G/farmacocinética , Cromatografía Líquida de Alta Presión/métodos , Medios de Cultivo , Estabilidad de Medicamentos , Filtración/métodos , Concentración de Iones de Hidrógeno , Técnicas In Vitro , Penicilina G/administración & dosificación , Soluciones , Temperatura , Agua/farmacologíaRESUMEN
A possibility of complete separation of fine disperse admixtures from antibiotic fermentation broth filtrates by filtration with the use of special additives was shown. The purity of the filtrates and filtration rate depended on the procedure of addition, amount and average size of the particles of the filtration material.
Asunto(s)
Antibacterianos/aislamiento & purificación , Contaminación de Medicamentos , Filtración/instrumentación , Matemática , Penicilinas/aislamiento & purificación , Proyectos de Investigación , SolucionesRESUMEN
Main filtration characteristics of the fermentation broths are necessary for estimation of the filtration equipment, rational choice of the filtering apparatus, determination of the optimal conditions for their exploitation. In this connection studies were carried out with a purpose of determining the specific resistance and the content of the solid phase in the fermentation broths of the main antibiotics. It was shown that the above characteristics were closely connected with the biosynthetic conditions and medium composition. It was noted that the specific resistance of the precipitates of the fermentation broths of various antibiotic-producing organisms significantly differed, sometimes by several orders. Preliminary treatment of the fermentation broths before filtration provided a marked decrease in the resistance of the precipitate.